基于线粒体控制区部分序列的南海大斑石鲈遗传多样性分析

为了研究南海大斑石鲈(Pomadasys maculatus)不同地理群体的遗传多样性情况,作者测定了东兴、乌石、潭门、闸坡4个群体共计61尾大斑石鲈控制区的993 bp序列。检测出变异位点35个,单倍型47种,平均单倍型多样性指数为0.9884,核苷酸多样性指数为0.0076,总体表现出高单倍型多样性和低核苷酸多样性的特点,其中潭门群体核苷酸多样性相对较高(0.00879)。中性检验结果显示Fu’s Fs值均为显著负值–5.34(P=0.03),核苷酸不配对分布没有显著偏离群体扩张模型呈现出单峰(SSD值和Rg指数较小),表明大斑石鲈在历史上经历过种群扩张,推测扩张时间约4.74万~1.18万年间。群体间(0.0065~0.0089)与群体内遗传距离(0.0066~0.0089)处于同一水平,总遗传分化指数Fst为–0.0057(P0.05),群体间基因流Nm=33.76。不同组群划分方式的分子方差分析均表明群体内遗传差异显著大于群体间,遗传变异主要来源于群体内部。南海海域大斑石鲈群体遗传多样性匮乏,群体间不存在显著的遗传分化,可划归一个管理保护单元,潭门群体建议优先给予保护。     

Lagged response of phytoplanktonic photosynthetic competence to variation of nitrogen concentration in Jiaozhou Bay, China

Fluorescence-based maximal photochemical efficiency, Fv/Fm, is widely used as an indicator to photosynthetic competence in marine systems. It has been considered a useful parameter diagnosing the nutrient stress on phytoplankton photosynthesis, but many studies argue its usefulness. In the present study, we try to find a temporal relationship between Fv/Fm and nitrogen concentration, and provide a possible explanation on the controversy. We continuously measured Fv/Fm and nitrogen concentration once every 10 days from September 2003 to March 2004 at two stations in Jiaozhou Bay, northern China. It was found that Fv/ffm did not significantly correlate to synchronous nitrogen concentration, but the variation （i.e. the change between two adjacent cruises） of nitrogen concentration of the previous cruise and the variation ofFv/Fm of the current cruise were strongly correlated. This result indicates that a time lag exists between the variation of nutrient status and the subsequent Fv/Fm response. Length of the time lag seems just matched the interval of our measurements （10 days）. In the field, direct dependence of Fv/Fm on nitrogen concentration may not be found because of the lagged response of Fv/Fm to nitrogen concentration variations or physiological acclimation. Our results provide a possible way to explain the previously reported conflicting results on the relationship between Fv/Fm and nutrient status. To give a more-accurate estimate about the length of the time lag, an investigation that includes more frequent measurements is needed.     

Response of cyanobacterial carbon concentrating system to light intensity: a simulated analysis

Cyanobacteria possess a delicate system known as the carbon concentrating mechanism (CCM), which can efficiently elevate the intracellular inorganic carbon (Ci) concentration via active transportation. The system requires energy supplied by photosystems; therefore, the activity of the Ci transporter is closely related to light intensity. However, the relationship between CCM and light intensity has rarely been evaluated. Here, we present an improved quantitative model of CCM in which light is incorporated, and developed a CCM model that modified after Fridlyand et a1. in 1996. Some equations used in this model were inducted to describe the relationship between transport capacity and light intensity, by which the response of the CCM to light change is simulated. Our results indicate that the efficiency of the carbon concentrating system is sensitive to light intensity. When the external Ci concentration was low, CO2 uptake dominated the total Ci uptake with increasing light intensity, while under high external Ci concentrations HCOˉ3 uptake primarily contributed to the total Ci uptake. Variations in the ratio of energy allocated between the transport systems could markedly affect the operation of CCM. Indeed, our simulations suggest that various combinations of Ci fluxes can provide a possible approach to detect the way by which the cell distributes energy produced by the photosystems to the two active Ci transport processes. The proportion of the energy consumed on CCM to the total energy expenditure for the fixation of one CO2 molecule was determined at 18%–40%.     

热带水库敞水区轮虫种类组成与数量结构—基于海南7座典型水库的分析

海南省是我国典型的热带区域,目前对该地区浮游动物的研究和报道极少.为了解该地区轮虫的群落组成,于2006年12月和2007年5月对分布于海南省7座典型的水库敞水区进行了采样调查.共采集到轮虫32种,多为营浮游性生活的广温性和嗜温性种类.在不同时期,水库具体优势种类及其相对优势度有一定差别,主要优势种个体较小且有被甲.在丰水期,剪形臂尾轮虫和镰状臂尾轮虫为主要优势种,在枯水期则以柬隐三肢轮虫为主要优势种.海南省7座水库的平均丰度和生物量分别为66.8ind./L和21.6μg/L,均远低于亚热带和温带地区.总体上,海南省水库敞水区轮虫呈现出种类少、个体小、丰度低的特点.尽管两次采样期间的轮虫优势种的相对丰度存在一定变化,但轮虫的种类组成结构相对一致,反映出该地区水库轮虫的群落结构具有较高稳定性.     

四种浮游植物生物量计算方法的比较分析

浮游植物是水生生态系统中重要的初级生产者,其生物量是反映其现存量的关键指标.本文利用具有3个处理组的围隔实验中的浮游植物数据,对文献中常见的计算浮游植物种群生物量和群落生物量的4种方法:标准法、细分法、粗分法和资料法进行比较,并分析采用这4种不同方法得到的浮游植物生物量与叶绿素a浓度的相关性.结果表明:粗分法是计算浮游植物生物量的高效方法,能够保证准确度和节省时间;提高浮游植物生物量计算准确度不是影响浮游植物生物量与叶绿素a浓度相关性显著程度的关键.通过比较剔除稀有种(生物量不超过群落生物量5%的种类)前后浮游植物生物量的差异,发现忽略稀有种会导致种类均匀度较高的浮游植物群落生物量严重偏低,建议浮游植物生物量的计算不能一概忽略稀有种.     

广东流溪河水库盔型溞(Daphnia galeata)休眠种群与现生种群的单倍型多样性和遗传分化

休眠卵库作为淡水枝角类生物与遗传信息的储藏库,从沉积物休眠卵库中萌发的枝角类对现生种群的数量与种群遗传结构有着直接的影响.分别采集流溪河水库盔型溞的现生种群和沉积物表层(0~10 cm)的休眠卵,扩增现生种群与休眠卵的线粒体细胞色素C氧化酶亚基I基因,构建了单倍型网络.休眠种群相比现生种群有着较高的单倍型多样性和核酸多样性,初期现生种群分别为0.562、0.00104,末期现生种群分别为0.726、0.00331,休眠种群分别为0.815、0.00761.流溪河盔型溞现生种群与休眠种群存在双向基因流,现生种群到休眠种群的有效迁移率为490.9,休眠种群到现生种群的有效迁移率为527.5.通过构建贝叶斯系统树验证了休眠种群和现有种群中并不存在隐种或者亚种的分化,休眠种群与现生种群之间没有出现较大的遗传分化,现生种群遗传多样性来自于休眠种群,水库的休眠种群更能反映种群真实的遗传多样性.休眠种群与现生种群之间的基因流与休眠卵库大小无关,与休眠卵的萌发有关.     

两种提取枝角类休眠卵基因组DNA方法的比较

休眠卵是枝角类在环境胁迫条件下通过有性生殖形成的,休眠卵的孵化对种群补充与恢复有着重要作用.沉积于底泥中的休眠卵保存了不同阶段的种群遗传信息,有效地提取枝角类休眠卵基因组DNA是进一步研究水体枝角类遗传多样性的关键环节.采用目前国际上使用的氯仿异戊醇提取法和玻璃乳提取法分别对流溪河水库底泥中表层和底层的盔型溞(Daphnia galeata)休眠卵进行基因组DNA提取的比较分析.结果显示,氯仿异戊醇提取法成功率为27.5%,平均浓度为14.25±1.84 ng/μl;玻璃乳提取法成功率为65.0%,平均浓度为28.37±2.56 ng/μl.无论是提取成功率还是提取浓度玻璃乳提取法都显著高于氯仿异戊醇提取法,且玻璃乳提取法所用试剂少,不涉及有毒试剂,操作步骤简单,整个提取过程所用时间短,玻璃乳提取法提取枝角类休眠卵基因组DNA是一种快捷实用的方法.     

广东省水库枝角类组成特征的初步研究

于2000年丰水期和枯水期调查了广东省19座典型大中型水库枝角类,随后对其中3座代表性水库进行了4年的枝角类种类定性调查.在鉴定出的枝角类16属24种中有13个广温种、7个嗜暖种、3个嗜寒种和1个特有种;每个水库的平均种类数5-6种.从个体大小来看,水库枝角类以小个体种类为主,有14种的体长小、于1 mm,它们分布广且数量多,其中优势种主要为长额象鼻溞、秀体溞、微型裸腹溞、颈沟基合溞和角突网纹溞等.透明溞和透明薄皮溞是主要的大个体种类,出现在4座贫、中营养水库的水样中.水库枝角类的丰度较低,在丰水期,平均丰度小于10 ind./L和10-20 ind./L的水库分别为14座和5座,在枯水期,为12座和5座,而高州水库和河溪水库中的平均丰度达67ind./L.主要优势种在不同营养类型水库中分布差别不大.枝角类的丰度受水力滞留时间影响,直流型水库的枝角类种群丰度的分布明显受到水流速度的影响,湖泊区枝角类个体及其食物均随水库放水输出而减少.由于采样点主要位于大坝附近,枝角类以敞水区的种类为主.从种类组成和数量来看,广东省水库枝角类具有明显的热带特点,枝角类的生物量小,以个体较小的种类优势种,大型种类的个体数量极低且身体透明.     

海南热带水库敞水区浮游甲壳动物群落结构特征

于枯水期(2006年12月)和丰水期(2007年5月)对海南省7座水库敞水区进行采样调查,共检出浮游甲壳类动物15种.多为广温性和嗜热性种类,其中桡足类7种,枝角类8种.温中剑水蚤(Mesocyclops thermocyclopoides)、脆弱象鼻溞(Bosmina fatali)和颈沟基合溞(Bosminopsi...     

广东省高州水库水华过程中蓝藻群落的动态特征

2009年1-4月,高州水库发生了规模较大的粘质鱼腥藻(Anabaena mucosa)水华,最高藻细胞密度达3.4×107cells/L,并在2010年的同一时期再次出现,这也是粘质鱼腥藻首次在广东省水库水体形成水华.为了解该水库蓝藻水华发生的特点,本文于2009年8月到2010年7月对高州水库进行了逐月采样,分析了...