Lichtenstein法无张力腹股沟疝修补术治疗体会

目的:探讨用聚丙烯平片进行腹股沟无张力疝修补术的手术要点,减少术后并发症及复发,提高患者生活质量。方法:回顾性总结用成都创盛生物医学材料有限公司生产的麦特适平片行Lichtenstein法进行腹股沟疝修补术178例。结果:本组病例均治愈。其中:出现缝线反应2例,阴囊血肿4例,经对症处理后痊愈,平均住院5～7d。结论:Lichtenstein法是腹股沟疝手术治疗的一种有效手段,值得推广。     

Primary culture of retinal Müller cells in adult rats assisted by the peritoneal exudative cells

Objective To investigate the effect of peritoneal exudative cells as feeder cells on growth from adult rats, which were identified with specifically biological marker of maerophage (CD68). The by enzyme digestion method, and identified by GFAP and vimentin immunocytochemicaUy. As the feeder cells. Results Over ninety-five percent of rat peritoneal exudative cells were macrophage, which have a flask and grew fast, with large applanate cell bodies. The third-generation cells grew slowly. After cocuhured with feeder cells, the Moiler cells showed more rapid growth rate with more cells in S and G2/M phase(S phase, t=4. 172, P<0.001; G2/M phase, t=3. 562, P<0.01)and less apoptotic rate (t =3. 804, P<0. 01). The growing cycle was cut down from 25-30 days to 18-22 days for the first-generation cells, from 10-15 days to 7-10 days for the second-generation cells. Conclusion It is an effective method to use the peritoneal exudative cells as feeder cells cocuhured with primary culture of retinal Müller cells,which can shorten the culture period of Müller cells in adult rats.     

豚鼠形觉剥夺性近视眼视网膜、脉络膜多巴胺代谢的变化

目的研究形觉剥夺对豚鼠神经视网膜、视网膜色素上皮（retinal pigment epithelium,RPE）／脉络膜复合体多巴胺（dopamine,DA）代谢的影响。方法28日龄豚鼠36只,分为3组：正常对照组、遮盖组、遮盖＋去遮盖组,每组12只。遮盖组用半透明眼罩遮盖豚鼠右眼14d,遮盖＋去遮盖纽在遮盖11d后去遮盖3d。14d后测定角膜曲率半径、眼球屈光度和眼轴长度。处死豚鼠,取眼后极部视网膜、脉络膜组织,用免疫组化染色和Western blotting法检测酪氨酸羟化酶（tyrosine hydroxylase,TH）蛋白在神经视网膜、RPE／脉络膜复合体的表达情况,用高效液相色谱检测神经视网膜、RPE／脉络膜复合体的DA、二羟基苯乙酸（3,4-dihydroxyphenylacetic acid,DOPAC）的含量。结果TH蛋白阳性表达于视网膜神经节细胞和内核层部分细胞,RPE／脉络膜复合体中表达阴性。遮盖14d后,豚鼠遮盖眼眼轴延长,近视形成,神经视网膜TH蛋白表达量和DA、DOPAC含量降低（P〈0．05）。遮盖＋去遮盖纽的遮盖眼近视程度低于遮盖组．其神经视网膜TH蛋白表达量和DA、DOPAC含量升高（P〈O．05）,但仍低于正常对照组（P〈O．05）。各纽豚鼠RPE／脉络膜复合体的DA、DOPAC含量比较,差异无统计学意义（P〉0．05）。结论形觉剥夺能调控豚鼠神经视网膜DA代谢,但不影响RPE／脉络膜复合体的DA代谢。     

体外培养人眼小梁细胞Ang Ⅱ的表达

目的 探讨血管紧张素Ⅱ(AngⅡ)在体外培养的人眼小梁细胞(TMC)中的表达情况.方法 原代和传代培养人眼TMC株,收集第3代人眼TMC用于制作细胞爬片.利用免疫组织化学染色法和Western blot法检测体外培养人眼TMC中AngⅡ蛋白的表达.结果 传代培养的人眼TMC呈典型梭形,免疫组织化学检测显示体外培养的人眼TMC细胞膜和细胞质中可见AngⅡ的阳性颗粒,阴形对照片中未见有AngⅡ蛋白表达.Western blot法在相对分子质量为64 000处可见一明显蛋白条带,为AngⅡ蛋白的阳性表达.结论 AngⅡ在体外培养的人眼TMC中呈阳性表达,提示AngⅡ可能参与青光眼眼压和房水循环的调节.     

异体巩膜植入联合“Z”形皮瓣治疗陈旧性外伤性下睑退缩

目的探讨异体巩膜植入联合“Z”形皮瓣治疗陈旧性外伤性下睑退缩的临床疗效。方法伤后5—14个月用异体巩膜植入联合“Z”形皮瓣治疗12例（12眼）陈旧性外伤性下睑退缩,术后自静脉及滴眼途径给予抗感染抗炎治疗。结果术后随访8—12个月,9眼单次手术后获得良好的外观和功能恢复,1眼经2次手术后获得满意的外观和功能,另2眼（16．67％）单次手术后效果不佳但未再手术治疗。结论异体巩膜植入联合“Z”形皮瓣是治疗陈旧性外伤性下睑退缩的一种有效方法。     

不同用药途径促红细胞生成素用于治疗大鼠急性脊髓损伤的研究

目的：观察经不同给药途径给予重组人红细胞生成素(EPO)治疗脊髓损伤后大鼠神经功能行为,caspase-3表达以及凋亡的变化,探讨其脊髓保护的作用机制。方法：30只SD大鼠随机分成假手术组、对照组、蛛网膜下腔给药组(治疗A组),尾静脉给药组(B组),腹腔给药组(C组),每组6只。观察药物治疗前后大鼠的神经功能行为变化,应用HE染色观察神经元病理变化,免疫组化染色检测caspase-3表达,TUNEL法标记凋亡细胞;比较各组间差异。结果：HE,免疫组化染色示各EPO治疗组脊髓损伤程度小,神经元细胞破坏少;c     

MRI监测不典型发病的实验性自身免疫性脑脊髓炎

目的 利用MRI监测实验性自身免疫性脑脊髓炎(EAE)发病进程,判断MRI对不典型发病的实验性自身免疫性脑脊髓炎的监测价值.方法 应用MOG35-55诱导Lewis大鼠建立EAE模型.对所有EAE大鼠进行急性期、慢性期的神经功能评分、MR常规成像及USPIO强化成像,比较MRI与临床状态的关系.处死部分EAE大鼠进行病理分析,并与MRI进行对照.结果 MOG35-55诱导的EAE模型症状复杂多样,神经功能评分与MRI对病情的判断存在差异,MRI可以发现、但神经评分不易判断的EAE病灶.结论 MRI可提高对不典型发病的EAE病灶的检出率,能更加客观、全面地评估EAE模型.     

内蒙古林区蜱咬人群人粒细胞埃里希体病DNA检测

人粒细胞埃里希体病,是1994年美国发现的嗜人类粒细胞埃里希体(human granulocgtic ehrlichia)引起的人粒细胞埃里希体病,一些硬蜱是其主要传播媒介,其临床表现主要有发热、寒战、不适、肌痛、头痛等类感冒症状和恶心、呕吐、意识模糊等,严重者出现肾功能衰竭和中枢神经系统受损。甚至死亡。2001年黑龙江省林区的全钩硬蜱中检测出人粒细胞埃里希体病原体DNA,     

Primary culture of retinal Müller cells in adult rats assisted by the peritoneal exudative cells

Objective To investigate the effect of peritoneal exudative cells as feeder cells on growth from adult rats, which were identified with specifically biological marker of maerophage (CD68). The by enzyme digestion method, and identified by GFAP and vimentin immunocytochemicaUy. As the feeder cells. Results Over ninety-five percent of rat peritoneal exudative cells were macrophage, which have a flask and grew fast, with large applanate cell bodies. The third-generation cells grew slowly. After cocuhured with feeder cells, the Moiler cells showed more rapid growth rate with more cells in S and G2/M phase(S phase, t=4. 172, P<0.001; G2/M phase, t=3. 562, P<0.01)and less apoptotic rate (t =3. 804, P<0. 01). The growing cycle was cut down from 25-30 days to 18-22 days for the first-generation cells, from 10-15 days to 7-10 days for the second-generation cells. Conclusion It is an effective method to use the peritoneal exudative cells as feeder cells cocuhured with primary culture of retinal Müller cells,which can shorten the culture period of Müller cells in adult rats.     

终末期肾病甲状腺激素变化的临床意义

本文测定60例终末期肾病的甲状腺激素水平,并比较非透析治疗、血透和腹透等不同治疗方法的影响。结果发现终未期肾病血TT_3、TT_4显著下降;TSH 在正常范围。非透析治疗组血TT_3和Cr 呈负相关,提示甲状腺激素的变化和肾衰竭的严重程度有关。血TT_3在血透组和腹透组相似,而TT_4腹透组高于血透组。本文对其临床意义作了讨论。