ST14/MT-SP1 influences expression of MT2-MMP and TIMP-2 to promote invasiveness of colorectal cancer cells

AIM: To evaluate how ST14/MT-SP1 overexpression alters invasiveness of colorectal cancer cells.METHODS: Human full length ST14/MT-SP1 gene was transiently transfected into colorectal cancer (RKO) cell lines.The expression products were purified by chromatography on Ni2+-affinity resin column and analyzed for gelatinase activity by gelatin zymography.Cell invasion and migration were determined by ECM invasion assay in vitro.RNA was isolated from stable ST14-transfected RKO cells and a cDNA microarray was utilized to detect the change in expression of MMPs and TIMPs.Real-time quantitative PCR was used to validate the change of expression.RESULTS: The full length ST14/MT-SP1 was transfected and expressed in RKO cells.The expressed protein showed a gelatinase activity.In addition,invasiveness of RKO was significantly enhanced by ST14/MT-SP1 overexpression (P<0.01).Blockage of ST14/MT-SP1 resulted in a decrease in the invasiveness of SW480 and SW620 (P<0.01).Furthermore,MT2-MMP (MMP-15) expression was significantly up-regulated (2.5-fold change) and TIMP2 down-regulated (0.35-fold change) by ST14/MT-SP1 overexpression in RKO.CONCLUSION: ST14/MT-SP1 overexpression results in an increase in invasiveness of colorectal cancer RKO cells.Increased invasiveness is due to an increase in the gelatinase activity of ST14/MT-SP1 and a change in up-regulated MT2-MMP and down-regulated TIMP-2 expression.Therefore,ST14/MT-SP1 overexpression enhances invasiveness of colorectal cancer cells.     

平邑甜茶后代双胚苗的倍性鉴定及核型分析

为了确定平邑甜茶双胚苗的倍性特征和核型特点,采用压片和流式细胞仪观测的方法对平邑甜茶后代中双胚苗进行染色体倍性观察和核型分析。结果表明:4株供试材料均为三倍体,2n=3x=51,染色体为小型,染色体长度比差异不大;核型组成由中部着丝点染色体(m)和亚中部着丝点染色体(sm)组成;株系8-1、8-2、12-1和12-2的核型公式分别为2n=3x=45m+6sm,2n=3x=48m+3sm,2n=3x=27m+24sm,2n=3x=33m+18sm;染色体相对长度和着丝点指数的变异范围分别为1.21%～2.68%和34.19%～42.37%,1.44%～2.63%和35.59%～44.08%,1.36%～3.02%和32.85%～42.18%,1.43%～2.87%和34.23%～44.02%;核型分别属于1B、1A、2B和1B类型,表现为株系之间的核型不完全相同。本研究为丰富无融合生殖型砧木的育种理论,获得新的苹果无融合生殖型砧木提供有益的借鉴。     

New Varieties of Populus tomentosa Carr. and Breeding Techniques

The main morphological characteristics, producing area and growth characteristics of sixteen new varieties of Chinese white poplar (Populus to-mentoso Carr. ) are reported, aiming at providing referernce for breeding and extension of new varieties.     

动画演示方法在园林多媒体教学中的运用

本文探索了一种将动画演示应用于园林专业课程中的现代教学方法。从动画演示方法的适用对象与范围、动画制作的具体方法、动画在PPT中的调用等三个方面,详细介绍了该方法的使用理论与实际操作,并提出了影响动画演示的细节问题。最后,提出了该方法存在的一些问题,为其进一步发展与完善提供了思路。     

Preliminary Research on Regional Material Flow Analysis： A Case Study of Chengyang District in Qingdao

The method ＂Material Flow Analysis （MFA）＂ is one of the effective tools to study law and quantification of material flow between economic system and ecological system. On the national level, economy-wide material flow analysis has been published for a number of countries. However, published studies on the regional or local level are still very limited and a standardized method does not exist yet. On the basis of framework of Material Flow Analysis proposed by Eurostat, the paper collected related data and analyzed material input and output for the Chengyang district in Qingdao. The results showed that DMI （Direct Material Input） and TMR （Total Material Requirement） in absolute number increased about 3.6 and 3.9 times, respectively from 1995 to 2004. Fossil fuel and mineral contributed to about 50.3%-76.3% of DMI. Imports of material increased about 5.0 times and became the most important Contributor to DMI, which showed that local economic growth was highly dependent on resources from other regions and countries. DPO （Domestic Processed Output） and TDO （Total Domestic Output） represent slow increasing trend, and DPO contributed to 22.2%-58.1% of TDO, suggesting local hidden flows were of obvious effect on TDO. The biggest component of DPO is CO2, approximately accounting for 90% of DPO. The material productivity increased 57.7% in last decade, reflecting improvement of efficiency of resources utilization in some extent. However, compared to developed countries and regions, material productivity of Chengyang district was relatively low. Therefore, in order to promote the sustainability, it is essential to develop circular economy and enhance materials productivity.     

外源木聚糖酶对异育银鲫生长、超氧化物歧化酶及溶菌酶活性的影响

在基础饲料中分别添加0 mg/kg、50 mg/kg、100 mg/kg和200 mg/kg的木聚糖酶,饲养初始体重6.71 g左右的异育银鲫(Carassius auratus gibelio)56 d后,研究了木聚糖酶对异育银鲫生长、超氧化物歧化酶(SOD)和溶菌酶活性的影响。结果表明:添加100 mg/kg木聚糖酶组异育银鲫的增重率和特定生长率最大,显著大于对照组(P<0.05),同时该组异育银鲫的饲料系数最低,显著低于对照组(P<0.05)。添加50 mg/kg和100 mg/kg木聚糖酶可显著提高异育银鲫血清、脾脏、肝胰脏、头肾SOD活性以及血清、头肾、脾脏溶菌酶活性。     

Antitumor effect of heat-killed lactobacilli adhered to cervical carcinoma cells

AIM: To observe the immune property of cervical carcinoma and antitumour effect of heat-killed lactobacilli adhered to HeLa cells. METHODS: Flow cytometry was used to analyze the expression of immune molecules on HeLa cells after adhered with heat-killed lactobacilli. MTT was used to analyze the cytotoxity of lymphocytes on cervical carcinoma cells. RESULTS: Adherence of heat-killed lactobacilli to HeLa cells did not affect the expression of MHC-I molecules (P>0.05), but enhanced the expression of CD80, CD86 significantly (P<0.05) in HeLa cells. Adherence of heat-killed lactobacilli to HeLa cells also enhanced the cytotoxity of NK cells. Compared to control, the cytotoxity of CTL to HeLa carcinoma cells was significantly enhanced by the adherence of heat-killed lactobacilli to HeLa cells (P<0.05). CONCLUSION: Heat-killed lactobacilli strengthen the antitumor effect on HeLa cells.     

杂交棉生产存在的问题与栽培技术对策

通过对湖北省杂交棉生产的调查和对有关资料的统计分析，剖析了目前杂交棉生产存在的问题；根据有关研究成果，针对性地提出了杂交棉生产应采取的的栽培技术对策。     

论长株潭城市群物流园区规划建设

合理的长株潭物流园区规划建设对长株潭城市群发展具有重要意义.通过分析长株潭物流园区规划建设现状与存在的问题,并经过数据分析,提出了长株潭城市群物流园区的合理选址、数量、规模与功能定位,从而减少城市交通与环境压力,节约园区土地使用,增强园区交通便利,提升园区规模与集聚辐射效应,为长株潭两型社会城市群一体化物流发展提供了决策参考.     

滨海地区水对社会经济影响评价指标体系的构建

在阐明滨海地区水对社会经济影响评价指标体系的研究目的、原则的基础上，根据滨海地区的特点，构建了含总目标层、领域层、准则层和指标层的四阶递阶层次结构指标体系。并以山东滨海地区龙口市为例，运用层次分析法进行指标筛选，确定了由水资源、社会、经济和生态环境4个领域、12个准则、34个指标组成的指标体系。该指标体系基本上涵盖了滨海地区水对社会经济影响的主要方面，可以比较全面客观地反映滨海地区水资源对经济社会的影响力。