野生稻抗病虫基因的挖掘和利用

本文综合国内外文献,简要介绍了野生稻抗病虫基因发掘、定位、克隆及育种应用研究的进展,讨论了野生稻优异抗病虫基因在水稻育种中的应用前景。     

苗木切根技术研究进展

精准培育的理念已经渗入到森林培育的各个环节。切根作为干扰根系最为重要的技术,也受到越来越多地重视。切根可以划分为芽苗切根、苗期切根和起苗后切根等3种类型,把平截、扭根、侧方修根、盒式修根等称为苗期切根,是狭义切根范畴。结合苗木形态指标、生理指标和造林效果,综述每一类型的切根对苗木质量和造林效果的影响。以辐射松为代表的主根发达树种,通过芽苗切根、苗期的平截和扭根,抑制根系顶端优势,从而降低苗木高生长,促进侧根发育,形成发达、紧凑的根系,调整苗木地上和地下比例,最终提高苗木造林效果。以花旗松为代表的慢生常绿树种,侧根较为发达,切根效果不一。苗木平截和扭根后,内源激素、矿质营养、碳水化合物、水分等均会发生变化,这种变化如何影响苗木质量的机制尚不明确。     

大豆水溶性蛋白含量测定的不确定度评定

建立大豆水溶性蛋白含量测定（NY/T 1205-2006）不确定度评定的数学模型,对检测过程中带来的各个不确定度分量进行识别和量化,提出应用该方法检测的不确定度评定结果。     

中山市资源节约型农业发展模式

本文以广东省中山市农业的发展为案例,定性分析了该地区发展节约型农业的模式,以及由此带来的经济效益、生态效益和社会效益。在对中山市农业进行科学评价的基础上,确定了该市节约型农业的主要发展模式为：节地模式的土地流转和提高耕地质量,节水模式的微灌,节肥模式的测土配方。     

滨水环境景观设计研究——以湖州太湖旅游度假区为例

湖州市是一座江南古城,有着浓厚的文化底蕴.湖州太湖旅游度假区位于湖州市境内,其区域内河道网络纵横交错,产生了众多的桥梁和滨水地带.为进一步提升湖州滨湖度假区环境品质,增加度假休闲氛围,打造国内一流休闲旅游度假区,现对滨湖休闲景现带的景观设计理念进行研究探讨.     

甜高梁秸秆酒糟复合饲料的初步研制

以甜高粱秸秆发酵乙醇后的酒糟作为原料,与其他饲料进行简单复配,制成了适口性好、价格低廉、具有一定饲用价值的粗饲料。其配制方法简单且易于推广,既补充了动物养殖的粗饲料,又提高了甜高粱秸秆的综合利用率,还可减小发酵企业对环境的污染。     

庐山自然保护区秋色叶树种观赏特性及其园林造景应用

介绍庐山保护区秋色叶树种观赏特性,从造景的角度,探讨秋色叶树种在园林中的应用,并提出开发对策。     

结合实际工作谈建筑信息模型（BIM）的意义及推广

论述了建筑信息模型（BIM）的概念,并结合实际工作说明了建筑信息模型（BIM）技术对建筑师、工程师日常工作的意义,提出了推广BIM的一些建议。     

Proapoptotic mechanism and changes of endogenous TGF-β1 in NB4 cells induced by exogenous TGF-β1

AIM: To study the effects of transforming growth factor-β1 (TGF-β1) on cell apoptosis, cell cycle, production of endogenous TGF-β1, expressions of P27Kip1, cyclin E and bcl-2 mRNA levels in NB4 cells. METHODS: Apoptotic morphological changes were observed by Wright-Giemsa staining. Cell cycle and apoptosis were detected with flow cytometry. Semiquantitative RT-PCR was used to examine the mRNA levels of endogenous TGF-β1, P27Kip1, cyclin E and bcl-2. RESULTS: TGF-β1 significantly restrained the growth and promoted the apoptosis of NB4 cells. The blockage of NB4 cells treated by TGF-β1 at concentration of 5 μg/L was in G1 phase. Endogenous TGF-β1 mRNA expression in NB4 cells was up-regulated when the concentration of exogenous TGF-β1 was <5 μg/L. Meanwhile, the expression of endogenous TGF-β1 mRNA was down-regulated when the concentration of exogenous TGF-β1 was 10 μg/L. After treated with TGF-β1 at concentration of 5 μg/L, P27Kip1 mRNA expression in NB4 cells was up-regulated, cyclin E and bcl-2 were reduced. CONCLUSION: TGF-β1 is able to induce apoptosis and cell cycle distribution abnormally in NB4 cells by (1) Up-regulation of endogenous TGF-β1, so that NB4 cells was induced into apoptosis through consequently high expression of P27Kip1. (2) TGF-β1 may lead to cell cycle arrest by inhibiting the expression of cyclin E directly, or by inhibiting the activity of cyclin E through the increased expression of P27Kip1. (3) Down-regulation of bcl-2 induces apoptosis of NB4 cells.     

Establishment of Efficient Regeneration System of Populus×euramericana cl.‘Bofeng’

Compared with the species of Leuce section,the difficulty in plant regeneration during tissue culture for the species of Aigeiros section is a key limiting factor for their application in genetic engineering of forest trees.In this study,the plant growth regulators combination,copper concentration,light intensity,and the selection pressures of kanamycin were investigated using leaves of Populus×euramericana cl.’Bofeng’ as explants,and a stable and high efficient regeneration system was established.Using this system,both the shoot regeneration rate and rooting rate were up to 100%,and the average number of differentiated shoots in each leaf explant was up to 20,and the survival rate of seedlings reached 98%.The optimal selection pressure of kanamycin in shoot inducement for leaf-explant was 40 mg·L^-1,and the optimal selection pressure of kanamycin in root inducement of adventitious bud was 20 mg·L^-1.The authors also found that differentiation rate of adventitious buds of P.×euramericana cl.’Bofeng’ could be significantly promoted by increased copper concentration,and this is the first report about the key role of copper（Cu） in explant regeneration of Poplars.This regeneration system with high frequency which was established by the authors was as good as that of species of Leuce section,and it provides a good technological platform for genetic engineering of forest tree which uses the species of Aigeiros section as model materials.