Simulation of 1D surface and 2D subsurface water flow and nitrate transport in alternate and conventional furrow fertigation

Increasing water and fertilizer productivity stands as a relevant challenge for sustainable agriculture. Alternate furrow irrigation and surface fertigation have long been identified as water and fertilizer conserving techniques in agricultural lands. The objective of this study was to simulate water flow and fertilizer transport in the soil surface and in the soil profile for variable and fixed alternate furrow fertigation and for conventional furrow fertigation. An experimental data set was used to calibrate and validate two simulation models: a 1D surface fertigation model and the 2D subsurface water and solute transfer model HYDRUS-2D. Both models were combined to simulate the fertigation process in furrow irrigation. The surface fertigation model could successfully simulate runoff discharge and nitrate concentration for all irrigation treatments. Six soil hydraulic and solute transport parameters were inversely estimated using the Levenberg–Marquardt optimization technique. The outcome of this process calibrated HYDRUS-2D to the observed field data. HYDRUS-2D was run in validation mode, simulating water content and nitrate concentration in the soil profiles of the wet furrows, ridges and dry furrows at the upstream, middle and downstream parts of the experimental field. This model produced adequate agreement between measured and predicted soil water content and nitrate concentration. The combined model stands as a valuable tool to better design and manage fertigation in alternate and conventional furrow irrigation.     

Effect of Bone Marrow Mesenchymal Stem Cell Sheets on Skin Capillary Parameters in a diabetic wound model: A Novel Preliminary Study

Background:Treatment with BMMSCs has anti-inflammatory, tissue regenerative, angiogenic, and immune-stimulating effects. When using as sheets or accumulate, BMMSCs causes the development of neoangiogenesis in damaged skin tissue. Diabetes, a metabolic disorder, can negatively affect many physiological functions, including the process of skin injury repair. This adverse impact may increase the risk of skin surgery. RSF is commonly used in reconstructive surgery. The terminal part of the RSF is often affected by necrosis because of impaired blood flow, which is exacerbated in diabetes. This study investigated the effect of stem cells, applied as accumulated or cell sheets, along with RSF surgery on skin capillaries in STZ-induced diabetic rats. Methods:Thirty male Wistar rats were divided into three groups (n = 10): diabetes-RSF control, diabetes-RSF local applied stem cells (loc-BMMSCs), diabetes-RSF applied stem cells as accumulated or cell sheets (ac-BMMSCs). Two weeks after the STZ injection, RSF surgery and stem cell therapy (6 × 10⁹) were carried out (day zero). Furthermore, stereological methods were used to investigate the capillary patterns among the groups. Anti-CD31/PCAM1 immunohistochemistry was also used for further confirmation of changes in capillary parameters. Results:The results demonstrated that capillaries were protected by MSC sheets in the flap tissue, and the thickness of the epidermal layer was improved, indicationg the possible beneficial effects of MSC sheets on diabetic wound treatment. Conclusion:Stem cells, as ac-BMMSCs, may decrease the levels of wound healing complications in diabetes and can be considered as a cell therapy option in such conditions.Key Words: Neoangiogenesis, Skin flap, Transplantation, Wound healing     

Mitochondrial DNA Copy Number Variations and Serum Pepsinogen Levels for Risk Assessment in Gastric Cancer

Background:Variations in mtDNA-CN of PBLs, as a potential biomarker for GC screening has currently been subject to controversy. Herein, we have assessed its efficiency in GC screening, in parallel and in combination with sPG I/II ratio, as an established indicator of gastric atrophy. Methods:The study population included GC (n = 53) and non-GC (n = 207) dyspeptic patients. The non-GC group was histologically categorized into CG (n = 104) and NM (n = 103) subgroups. The MtDNA-CN of PBLs was measured by quantitative real-time PCR. The sPG I and II levels and anti-H. pylori serum IgG were measured by ELISA. Results:The mtDNA-CN was found significantly higher in GC vs. non-GC (OR = 3.0; 95% CI = 1.4, 6.4) subjects. Conversely, GC patients had significantly lower sPG I/II ratio than the non-GC (OR = 3.2; CI = 1.4, 7.2) subjects. The combination of these two biomarkers yielded a dramatic amplification of the odds of GC risk in double-positive (high mtDNA-CN-low sPGI/II) subjects, in reference to double-negatives (low mtDNA-CN-high sPGI/II), when assessed against non-GC (OR = 27.1; CI = 5.0, 147.3), CG (OR = 13.1; CI = 2.4, 72.6), or NM (OR = 49.5; CI = 7.9, 311.6) groups. Conclusion:The combination of these two biomarkers, namely mtDNA-CN in PBLs and serum PG I/II ratio, drastically enhanced the efficiency of GC risk assessment, which calls for further validations. Key Words: Biomarkers, DNA copy number variation, Mitochondrial DNA, Stomach neoplasms     

Evaluation of Outer Membrane Vesicles Obtained from Predominant Local Isolate of Bordetella pertussis as a Vaccine Candidate

Background:Pertussis is a current contagious bacterial disease caused by Bp. Given the prevalence of pertussis, development of new vaccines is important. This study was attempted to evaluate the expression of main virulence factors (PTX, PRN, and FHA) from Bp predominant strains and also compare the expression of these factors in the OMVs obtained from predominant circulating Bp isolate. Methods:The physicochemical features of the prepared OMVs were analyzed by electron microscopy and SDS-PAGE. The presence of the mentioned virulence factors was confirmed by Western blotting. BALB/c mice (n = 21) immunized with characterized OMVs were challenged intranasally with sublethal doses of Bp, to examine their protective capacity. Results:Electron microscopic examination of the OMVs indicated vesicles within the range of 40 to 200 nm. SDS-PAGE and Western blotting demonstrated the expression of all three main protective immunogens (PTX, PRN, and FHA), prevalent in the predominant, challenge, and vaccine strains, and OMVs of the predominant IR37 strain and BP134 vaccine strain. Significant differences were observed in lung bacterial counts between the immunized mice with OMV (30 CFU/lung) compared to the negative control group ((6 10⁴ CFU/lung; p < 0.001). In mice immunized with OMVs (3 µg), the number of lungs recovered colonies after five days dropped at least five orders of magnitude compared to the control group. Conclusion:OMVs obtained from circulating isolates with the predominant profile may constitute a highly promising vaccine quality. They also can be proposed as a potential basic material for the development of new pertussis vaccine candidate. Key Words: Bordetella pertussis, Vaccines, Virulence factors     

Growth parameters of whiteleg shrimp Litopenaeus vannamei and red seaweed Gracilaria corticata in integrated culturing method under zero water exchange system

Growth parameters of whiteleg shrimp Litopenaeus vannamei and red seaweed Gracilaria corticata were measured using integrated culturing method under zero‐water exchange system in a 45‐day period. A 2 × 3 factorial design was used with two levels of shrimp stocking densities and three levels of seaweed weight densities. G. corticata was cultured on a net tied to a round polyethylene frame. Culture tanks were filled with 750‐L filtered seawater. A 40‐W compact fluorescent lamp was hung over each tank to provide adequate and sufficient light for seaweed growth. Growth parameters of shrimp and seaweed such as specific growth rate (SGR), weight gained (WG) and average daily growth (ADG) were computed based on the initial and final weight of shrimp and seaweed. The maximum and minimum SGR of L. vannamei (1.97 and 1.69%/day) were observed in treatment S₁A₃ (25 shrimp/m² and 400 g seaweed/m²) and S₂A₁ (50 shrimp/m² without seaweed) respectively. The best survival rate (94.67 ± 1.33%), WG (129.9 ± 2.9%) and feed conversion ratio (1.67 ± 0.04) were also observed in treatment S₁A₃. The SGR of G.corticata in the treatment S₁A₃ (1.97 ± 0.00%/day) was significantly higher, compared to others. Strong positive correlations were obtained between the density of G. corticata and the growth parameters of L. vannamei. The red seaweed G. corticata could boost the growth parameters, survival rate and total production of L. vannamei under zero‐water exchange system.     

Evaluation of HYDRUS-2D model to simulate the loss of nitrate in subsurface controlled drainage in a physical model scale of paddy fields

Agriculture is a major source of nitrogen usage and release to environment. Due to the effect of water movement on solute transport, investigating the effect of different management scenarios of irrigation and drainage could be useful for reducing nitrate loss and environmental pollution. This study is a scientific attempt to assess the ability of HYDRUS-2D model to simulate the effect of subsurface controlled drainage on nitrate loss of paddy fields. So, two physical models with difference in depth of subsurface controlled drainage (40 and 60 cm) were constructed. The tanks were filled with loam silty soil texture and then transplanted rice. 90 kg/ha potassium nitrate fertilizer was added in two stages of rice growth. Mid-season drainage was applied 26 days after transplantation. After 17 days, drains were closed again and applied flooded irrigation with 5-cm water stagnant layer above soil surface. During experiment, nitrate concentration of drain water was measured. HYDRUS-2D was calibrated with measured data in 60 cm drain depth and validated with 40 cm drain depth. HYDRUS-2D could simulate nitrate concentration with the coefficient of determination 0.95 and 0.89 in calibration and validation stages, respectively. The comparison between the volume of drain water and nitrate concentration from the drains in the depths of 40 and 60 cm indicated lower nitrate load in depth of 40 cm. The results obtained proved that the presence of hardpan layer in depth of 25 cm rather than the absence of it causes increase in 3 % of average nitrate concentration and reduce in 17 % of water discharge.