Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages

Glutathione (GSH) concentrations of oocytes are considered as an important marker of the cytoplasmic maturation. The present study was designed to compare GSH concentrations of in vivo and in vitro matured canine oocytes. In vivo matured oocytes were collected 72 hr after ovulation by flushing fallopian tubes after laparotomy. Ovaries were collected from bitches with different reproductive stages, and collected oocytes were divided into 2 groups according to the size viz. < 120 microm and > 120 microm in diameter and cultured for 72 hr in Tissue Culture Medium-199 supplemented with 10% FBS, 2.2 mg/ml sodium bicarbonate, 2.0 microg/ml estrogen, 0.5 microg/ml FSH, 0.03 IU/ml hCG, and 1% penicillin-streptomycin solution in the presence or absence of 50 microM beta-mercaptoethanol. GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. GSH concentrations of immature canine oocytes were 2.9 and 3.8, 3.5 and 6.8, and 3.1 and 6.5 pM/oocyte for < 120 microm and > 120 microm in diameter oocyte groups at anestrous, follicular and luteal stage, respectively (P<0.05). In vivo matured oocytes had significantly higher GSH concentrations compared with in vitro matured oocytes. The GSH content was 19.2 pM/oocyte for in vivo matured oocytes, while 4.1 to 8.1 and 5.7 to 13.2 pM/oocyte for in vitro matured oocytes cultured in the absence or presence of beta-mercaptoethanol, respectively (P<0.05). Presence of beta-mercaptoethanol increased GSH synthesis in canine oocytes cultured in vitro, and oocytes collected from follicular and luteal stage was superior to anestrus oocytes.     

EXPERIMENTAL INVESTIGATION ON VIBRATION AND NOISE BY C6132 LATHE

This paper presents the principle of high-pass-absolute-value demodulation tcchnigue which is applied with spectrum analysis to investigate the vibration and noise produced by the C61 32 Lathe. The main noise sourse is found out and some reliable data are provided to decrease the noise and vibration and to improve the dynamic performence of the c6132 Lathe.     

单株幼龄苹果树修剪仿真研究

修剪是果树生产中重要的技术手段,要想研究更加合理的修剪技术体系,就必须保证能够对修剪反应有准确、直观和及时的判断,而仿真模拟技术提供了这种可能。以幼龄苹果树为研究对象,采用不同修剪处理对比的方法,在田间试验的基础上,总结修剪反应规律,构建苹果树结构和修剪模型。在此基础上基于Qt框架和OpenGL图形库,开发了单株苹果树修剪仿真软件平台。结合优化算法,通过仿真平台模拟了给定生产目标的修剪方案优化,优化结果对虚拟学习和实际生产提供了可视化指导。     

Effect of soil matric potential on tomato yield and water use under drip irrigation condition

Field experiment was carried out to investigate the effect of soil matric potential (SMP) on tomato yield, evapotranspiration (ET), water use efficiency (WUE) and irrigation water use efficiency (IWUE) under drip irrigation condition in North China Plain. The experiment included five treatments, which controlled SMP at 0.2 m depth immediately under drip emitter higher than −10 (S1), −20 (S2), −30 (S3), −40 (S4) and −50 kPa (S5), respectively, after tomato plant establishment. The results showed that different SMP affected irrigation amount and tomato ET. Irrigation amount decreased from 185 mm (S1) to 83.6 mm (S5) in 2004, and from 165 mm (S1) to 109 mm (S5) in 2005, respectively. The ET decreased from 270 mm (S1) to 202 mm (S5) in both years. However, it was found that SMP did not affect the tomato yield significantly, for the range of SMP investigated. Both WUE and IWUE increased as SMP decreased. The maximum WUE (253 and 217 kg/ha mm) and IWUE (620 and 406 kg/ha mm) were for S5 in 2 years, whereas the minimum WUE (178 and 155 kg/ha mm) and IWUE 261 and 259 kg/ha mm) were for S1 in 2004 and 2005. Based on the above results, therefore, it is recommended that if the tomatoes are well irrigated (SMP is higher than −20 kPa) during establishment, controlling SMP higher than −50 kPa at 0.2 m depth immediately under drip emitter can be used as an indicator for drip irrigation scheduling during following period of tomato growth in North China Plain.     

数据融合技术在温室温度检测中的应用

根据温室环境中空间大,温度场分布受到各种参数影响大等特点,提出了采用数据融合方法来处理温室中采集到的传感器数据,采用分布图法剔除疏失误差,采用基于均值的分批估计的融合方法对采集的数据进行处理,提高了温度采集的精度,同时有效地消除了由于传感器失效引起的误差。     

Effect of water deficit in different growth stages on stem sap flux of greenhouse grown pear-jujube tree

The effects of water deficit in different fruit growth stages on the variation of stem sap flux of 6-year old greenhouse-grown pear-jujube trees were investigated. Treatments included sufficient water supply during the whole fruit-growing period (T₁), mild water deficit during the flowering–fruit setting stage (T₂), moderate water deficit during the fruit rapid growth stage (T₃) and severe water deficit during the fruit maturing stage (T₄). Results showed that significant compensation effect on stem sap flux after re-watering was observed in T₂, but not in T₃ and T₄ stages. At the end of rapid growth stage, the diurnal variation of stomatal conductance generally had a similar trend as that of stem sap flux, but with a distinct midday depression from 12:00 to 14:00 p.m. In addition, a linear relationship between the relative available soil water content (RAWC) and the ratio of daily stem sap flux to that of sufficient water treatment was observed (R² = 0.4489).     

Drip irrigation scheduling for tomatoes in unheated greenhouses

During the last two decades, energy-saving solar greenhouses without heating systems, about 150-800 m² (width: 5-10 m, length: 30-80 m) in size, have been widely used in the North of China for vegetable production during cold seasons. The greenhouse is also suitable for use in other countries located in the temperate or warm-temperate zones, especially in the developing countries, because it is inexpensive with a simple structure, and does not need any additional energy for heating in winter. Drip irrigation has been recognized as a sensible method of supplying water inside the greenhouses, and a simple method for drip irrigation scheduling at low cost is urgently required. Using the water-balance method, the authors studied the water consumption of tomato (Lycopersicon esculentum Mill.) and the relationship between evapotranspiration and water surface evaporation measured with 20 cm pan. Research results show that there is no significant water flux at 0.75 m depth when soil water potential is kept higher than -20 kPa at 15 cm depth, and around -20 kPa at 60 cm depth. The accumulative value of evapotranspiration is approximately equal to the accumulative value of water surface evaporation measured using 20 cm pan. Further analysis found that 20 cm pan can be used to determine the water requirement of tomato drip irrigation scheduling in this type of greenhouse.     

冬小麦叶片水势、气孔阻力、蒸腾速率与环境因素的关系

本文依据田间试验资料,分析了冬小麦叶片水势、气孔阻力和蒸腾速率在不同土壤基质势条件下的日变化规律,探讨了其与土壤基质势和天气条件(光照强度、净辐射、饱和差、气温)之间的关系,建立了叶片水势、气孔阻力、蒸腾速率与环境因素间的定量关系式。