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排序方式: 共有156条查询结果,搜索用时 15 毫秒
101.
Dr. Masaya Kobayashi Prof. Dr. Tomohisa Kuzuyama 《Chembiochem : a European journal of chemical biology》2019,20(1):29-33
Terpenoids have diverse structures and thus represent an important class of biologically active natural products. The structural diversity of terpenoids originates from the coupling of prenyl diphosphate substrates, such as isopentenyl diphosphate and dimethylallyl diphosphate. These isoprenyl diphosphates undergo canonical and sequential “head-to-tail” coupling catalyzed by terpene synthases, followed by modifications such as cyclization, hydroxylation, and glycosylation. In recent years, several terpene synthases that catalyze irregular “non-head-to-tail” couplings to afford branched terpenoids have been identified. This minireview describes structural and mechanistic insights into these unusual coupling reactions that provide a new strategy for the structural diversification of natural products. 相似文献
102.
103.
Tomohisa Yoshioka Masakoto Kanezashi Toshinori Tsuru 《American Institute of Chemical Engineers》2013,59(6):2179-2194
The effect of the gas molecular size and its affinity to the pore surface on gas permeation properties through the ceramic membranes was studied by both the gas permeation experiments and gas permeation simulations using a nonequilibrium molecular dynamics (MD) technique. A modified gas permeation model equation based on the gas translation (GT) mechanism was presented. MD simulation revealed that the effective diffusion length in a micropore depended on the gas molecular size, and the pre‐exponential coefficient of a modified GT model equation showed good correlation with the kinetic diameter of the gas molecules. Also presented is a simple method to estimate the mean pore size of microporous membranes. The estimated pore sizes were consistent with observed kinetic diameter dependencies of gas permeance for real silica membranes. The pore size of a Deca‐Dodecasil 3R (DDR) zeolite membrane was also reasonably estimated at ~0.4 nm from the reported gas permeation data. © 2012 American Institute of Chemical Engineers AIChE J, 59: 2179–2194, 2013 相似文献
104.
Takeo Kimura Tomohisa Miyazawa Jin Nishikawa Shigeru Kado Kazu Okumura Toshihiro Miyao Shuichi Naito Kimio Kunimori Keiichi Tomishige 《Applied catalysis. B, Environmental》2006,68(3-4):160-170
Catalytic performance of Ni/CeO2/Al2O3 catalysts prepared by a co-impregnation and a sequential impregnation method in steam gasification of real biomass (cedar wood) was investigated. Especially, Ni/CeO2/Al2O3 catalysts prepared by the co-impregnation method exhibited higher performance than Ni/Al2O3 and Ni/CeO2/Al2O3 prepared by the sequential impregnation method, and the catalysts gave lower yields of coke and tar, and higher yields of gaseous products. The Ni/CeO2/Al2O3 catalysts were characterized by thermogravimetric analysis, temperature-programmed reduction with H2, transmission electron microscopy and extended X-ray absorption fine structure, and the results suggested that the interaction between Ni and CeO2 became stronger by the co-impregnation method than that by sequential method. Judging from both results of catalytic performance and catalyst characterization, it is found that the intimate interaction between Ni and CeO2 can play very important role on the steam gasification of biomass. 相似文献
105.
Abayomi P. Adebiyi Ayobamitale O. Adebiyi Dong-Hao Jin Tomohisa Ogawa & Koji Muramoto 《International Journal of Food Science & Technology》2008,43(3):476-483
The development of degradable and edible films from protein sources has drawn significant attention for the utilisation of natural resources as well as for the alleviation of the environmental burden. Rice bran protein (RBP) was applied to protein film preparation in this study. The protein solutions were casted on plastic tissue culture dishes with glycerol as a plasticiser after heat treatment. Functional properties of the films were then measured. The puncture strength (PS) of RBP films increased up to pH 8.0 and then decreased. PS of protein films depends on the degree of protein purity, quality and composition. Higher concentration of glycerol weakened the films. The pH affected the water solubility of RBP films and the films showed least solubility at pH 3.0. RBP could be utilised in the preparation of degradable protein‐based films. The RBP‐based film had functional properties comparable to those of the soy protein‐based ones. 相似文献
106.
Identification and Biosynthesis of New Acyloins from the Thermophilic Bacterium Thermosporothrix hazakensis SK20‐1T 下载免费PDF全文
Jin‐Soo Park Dr. Noritaka Kagaya Dr. Junko Hashimoto Dr. Miho Izumikawa Dr. Shuhei Yabe Dr. Kazuo Shin‐ya Prof. Makoto Nishiyama Prof. Tomohisa Kuzuyama 《Chembiochem : a European journal of chemical biology》2014,15(4):527-532
Two new acyloin compounds were isolated from the thermophilic bacterium Thermosporothrix hazakensis SK20‐1T. Genome sequencing of the bacterium and biochemical studies identified the thiamine diphosphate (TPP)‐dependent enzyme Thzk0150, which is involved in the formation of acyloin. Through extensive analysis of the Thzk0150‐catalyzed reaction products, we propose a putative reaction mechanism involving two substrates: 4‐methyl‐2‐oxovalerate as an acyl donor and phenyl pyruvate as an acyl acceptor. 相似文献
107.
Modified gas‐translation model for prediction of gas permeation through microporous organosilica membranes 下载免费PDF全文
Hiroki Nagasawa Takuya Niimi Masakoto Kanezashi Tomohisa Yoshioka Toshinori Tsuru 《American Institute of Chemical Engineers》2014,60(12):4199-4210
A modified gas‐translation (GT) model was applied for the theoretical analysis of gas permeation through microporous organosilica membranes derived from bis(triethoxysilyl)ethane (BTESE) via a sol–gel method using different water/alkoxide molar ratios. The pore sizes of BTESE‐derived membranes were quantitatively determined by normalized Knudsen‐based permeance analysis, which was based on a modified‐GT model, using experimentally obtained permeances of He, H2, N2, C3H8, and SF6. The pore sizes of BTESE‐derived membranes were successfully controlled from 0.65 to 0.46 nm by increasing the H2O/BTESE ratio from 6 to 240. Furthermore, theoretical correlations of all possible pairs of permeance ratios were calculated based on the modified‐GT model. The experimental data were in good agreement with the theoretical correlation curves, indicating that the modified‐GT model can clearly explain gas permeation mechanisms through microporous membranes, and, thus, can be used to predict the gas permeation properties for these membranes. © 2014 American Institute of Chemical Engineers AIChE J 60: 4199–4210, 2014 相似文献
108.
The amount of muddy soil generated from various kinds of construction sites is always problematic. It is very difficult to treat muddy soil because of its low strength and high water content. But, the reuse of muddy soil is necessary to reduce the total amount of industrial wastes. Surplus concrete is also in a similar situation. Coarse and fine aggregates are removed from surplus concrete as an intermediate treatment, however, concrete sludge still remains. The authors propose a reuse method that involves the muddy soil being mixed with concrete sludge as an improvement material. The possibility of the utilization of concrete sludge was investigated through laboratory experiments. As a result, it was found that the unconfined compressive strength of the improved soil mixed with concrete sludge increased as the curing proceeded. 相似文献
109.
We have previously demonstrated that blue light induces the phosphorylation of a 15-kDa protein in crude membrane fractions of Neurospora crassa mycelia. Here we report the isolation and characterization of a mutant (psp; phosphorylation of small proteins that is completely defective for phosphorylation of that protein, as assayed in both crude membrane and soluble fractions. This mutation defines a unique locus that maps to linkage group VR between al-3 and his-6. To elucidate the photobiological significance of the phosphorylation of the protein, we analyzed known photobiological phenomena and discovered that the positioning of beaks on the perithecia, defined as perithecial polarity, was light-dependent in the wild type. In the psp mutant, beaks were phototropic as in the wild type, but their position was random. In a wc-l mutant, however, beaks were positioned at random and were not phototropic. Thus light-induced perithecial polarity and phototropism of perithecial beaks are controlled differently. A psp; wc-l double mutant showed the same phenotype as that of wc-l with respect to these two photomorphogenetic characters. These results indicate that the wc-l gene is epistatic to psp in the light-signal transduction pathway that controls both phototropism and perithecial polarity. 相似文献
110.
Dr. Wataru Kitagawa Dr. Taro Ozaki Dr. Taiki Nishioka Dr. Yoshiaki Yasutake Miyako Hata Prof. Dr. Makoto Nishiyama Prof. Dr. Tomohisa Kuzuyama Prof. Dr. Tomohiro Tamura 《Chembiochem : a European journal of chemical biology》2013,14(9):1085-1093
Aurachin RE is a prenylated quinoline antibiotic that was first isolated from the genus Rhodococcus. It shows potent antibacterial activity against a variety of Gram‐positive bacteria. Here we have identified a minimal biosynthesis gene cluster for aurachin RE in Rhodococcus erythropolis JCM 6824 by using random transposon mutagenesis and heterologous production. The Rhodococcus aurachin (rau) gene cluster consists of genes encoding cytochrome P450 (rauA), prenyltransferase, polyketide synthase, and farnesyl pyrophosphate synthase, as well as others including genes involved in regulation and transport. Markerless gene disruption of rauA resulted in the complete loss of aurachin RE production and in the accumulation of a new aurachin derivative lacking the N‐hydroxy group. When the recombinant RauA was expressed in Escherichia coli, it catalyzed N‐hydroxylation of the derivative to form aurachin RE. This study establishes the biosynthetic pathway of aurachin RE and provides experimental evidence for the role of P450 RauA in catalyzing N‐hydroxylation of the quinoline ring, which is indispensable for the antibacterial activity of aurachin RE. 相似文献