Numerical simulation of atmospheric pollutant dispersion in an urban street canyon: Comparison between RANS and LES

Prediction accuracy of pollutant dispersion within an urban street canyon of width to height ratio W/H=1 is examined using two steady-state Reynolds-averaged Navier–Stokes (RANS) turbulence closure models, the standard k–ε and Reynolds Stress Model (RSM), and Large Eddy Simulation (LES) coupled with the advection–diffusion method for species transport. The numerical results, which include the statistical properties of pollutant dispersion, e.g. mean concentration distributions, time-evolution and three-dimensional spreads of the pollutant, are then compared to wind-tunnel (WT) measurements. The accuracy and computational cost of both numerical approaches are evaluated. The time-evolution of the pollutant concentration (for LES only) and the mean (time-averaged) values are presented. It is observed that amongst the two RANS models, RSM performed better than standard k–ε except at the centerline of the canyon walls. However, LES, although computationally more expensive, did better than RANS in predicting the concentration distribution because it was able to capture the unsteady and intermittent fluctuations of the flow field, and hence resolve the transient mixing process within the street canyon.     

Seasonal changes of α-tocopherol in green marine algae (Caulerpa genus)

Abstract: Marine algae are a promising source of beneficial compounds for human use. Among these, pro‐vitamin A carotenoids and vitamins B, C, and E stand out. The objective of this study was to investigate seasonal variation of α‐tocopherol levels in 5 species of green marine algae of the Caulerpa genus. This research was carried out with both fresh and dry specimens; and, in addition, differences arising as a result of the drying process were examined. Analyses were carried out by high‐performance liquid chromatography (HPLC) using an isocratic system and a reversed‐phase C‐18 column. The distribution of α‐tocopherol throughout the year in Caulerpa genus was variable. All samples of both fresh and dried algae contained α‐tocopherol, except for the dried C. racemosa from March 2006. The drying process was responsible for losses of α‐tocopherol ranging from 21% to 93%.     

Effects of pretreatment ultrasound bath and ultrasonic probe,in osmotic dehydration,in the kinetics of oven drying and the physicochemical properties of beet snacks

Osmotic dehydration and ultrasound are pretreatments used in order to reduce costs and processing time in the drying of food. We investigated the effect of the ultrasonic bath and an ultrasonic probe in osmotic solution, as a pretreatment of the drying process in an oven, for beet snacks. Different conditions of pretreatments (TP: ultrasonic probe treatment; TB: ultrasonic bath treatment) were performed and analyzed for water loss (WL) and solid gain (SG). After the snacks were ready, we evaluated the drying kinetics, aw, Brix, color, texture, and anthocyanin content. No difference was observed between treatments for SG and WL. The pretreatments TP5, TP10 and TB20 decreased by 22.2% the drying time in the oven. Regarding the snacks, there was no difference in texture. The colors of TP5, TP10, and TB10 were similar to the control, as well as the a_w value of the TP5 and TP10. The TP5 had the highest anthocyanin content. The use of ultrasound probe in osmotic solution for 5 min, as a predrying treatment, is a viable technology as it reduces pretreatment and drying time without impairing the quality of the final product.

Practical applications

The use of ultrasound as a pretreatment in the drying of fruits reduces the drying time. Drying is an important process in obtaining new products, in addition to increasing shelf‐life.     

Structural Characterization of the Interaction of Hypoxia Inducible Factor-1 with Its Hypoxia Responsive Element at the −964G > A Variation Site of the HLA-G Promoter Region

Human Antigen Leukocyte-G (HLA-G) gene encodes an immune checkpoint molecule that has restricted tissue expression in physiological conditions; however, the gene may be induced in hypoxic conditions by the interaction with the hypoxia inducible factor-1 (HIF1). Hypoxia regulatory elements (HRE) located at the HLA-G promoter region and at exon 2 are the major HIF1 target sites. Since the G allele of the −964G > A transversion induces higher HLA-G expression when compared to the A allele in hypoxic conditions, here we analyzed HIF1-HRE complex interaction at the pair-atom level considering both −964G > A polymorphism alleles. Mouse HIF2 dimer crystal (Protein Data Bank ID: 4ZPK) was used as template to perform homology modelling of human HIF1 quaternary structure using MODELLER v9.14. Two 3D DNA structures were built from 5′GCRTG’3 HRE sequence containing the −964G/A alleles using x3DNA. Protein-DNA docking was performed using the HADDOCK v2.4 server, and non-covalent bonds were computed by DNAproDB server. Molecular dynamic simulation was carried out per 200 ns, using Gromacs v.2019. HIF1 binding in the HRE containing −964G allele results in more hydrogen bonds and van der Waals contact formation than HRE with −964A allele. Protein-DNA complex trajectory analysis revealed that HIF1-HRE-964G complex is more stable. In conclusion, HIF1 binds in a more stable and specific manner at the HRE with G allele.     

Crystallization behavior of milk fat,palm oil,palm kernel oil,and cocoa butter with and without the addition of cannabidiol

The objective of this study was to evaluate the effect of cannabidiol (CBD) on the crystallization behavior and physical properties of various fats. Anhydrous milk fat (AMF), palm oil (PO), palm kernel oil (PKO), and cocoa butter (CB) were chosen for this study, for their unique crystallization behaviors. CBD was added at 1 and 2.5% wt/wt to these fats, and the crystallization behavior was evaluated at 26°C for AMF and PO and at 22°C for PKO and CB. Control samples with no CBD were prepared and evaluated as well. Results show that CBD delayed the crystallization of all fats with the least effect observed for the PO. Slight increases in crystal size were observed with the addition of CBD for all samples. CBD did not affect the melting profile of AMF or CB, but it increased the peak temperature of PO and decreased the enthalpy of PKO. Similarly, hardness was only affected by CBD in PO samples, with harder materials obtained for samples containing 2.5% CBD. The same trend was observed for elasticity. In addition, the elasticity of AMF increased with the addition of CBD but not its hardness. Overall, this study indicates that the effect of CBD on fat crystallization is highly dependent on the type of fat used. Producers of fat-based products that are willing to include CBD in their formulations must carefully control processing conditions to ensure product quality.     

Transesterification of waste cooking oil with a commercial liquid biocatalyst: Key information revised and new insights

The transesterification of the waste cooking oil (WCO) with various linear alcohols (C1 to C4) using the commercial biocatalyst Eversa® Transform in its liquid form is addressed in this investigation. The influence of the amount of the liquid formulation of lipase Eversa® Transform, nature of alcohol, oil to alcohol molar ratio and water addition in the transesterification of fresh sunflower oil and WCO was investigated. In addition, an innovative combination of that fungal based biocatalyst with a native vegetable lipase, known as Araujia sericifera (ASL), in a liquid formulation fashion was investigated. The assays carried out at 35°C for 24 h, an oil: alcohol molar ratio of 1:6.8, 2% v/w water added and 1% v/w of biocatalyst allowed to obtain up to 90% conversion and yield towards fatty acid methyl, ethyl and propyl esters. In the particular case of 1-butanol (C4) a 79% conversion and 72% yield to the esters was obtained. The biocatalyst maintains about 60% of its activity in the conversion of glycerides and yield towards the esters. The combination of process using Eversa® Transform and then the ASL lipase in the transesterification of the WCO probed efficient in the conversion of triglycerides using 1-butanol. A shift from 73 wt % of fatty acid butyl esters (FABE) towards 90 wt % was achieved.