Different bacterial models for in vitro induction of non‐cavitated enamel caries‐like lesions: Microhardness and polarized light miscroscopy analyses

The aim of this study was to compare different bacterial models for in vitro induction of non‐cavitated enamel caries‐like lesions by microhardness and polarized light microscopy analyses. One hundred blocks of bovine enamel were randomly divided into four groups (n = 25) according to the bacterial model for caries induction: (A) Streptococcus mutans, (B) S. mutans and Lactobacillus acidophilus, (C) S. mutans and L. casei, and (D) S. mutans, L. acidophilus, and L. casei. Within each group, the blocks were randomly divided into five subgroups according to the duration of the period of caries induction (4–20 days). The enamel blocks were immersed in cariogenic solution containing the microorganisms, which was changed every 48 h. Groups C and D presented lower surface hardness values (SMH) and higher area of hardness loss (ΔS) after the cariogenic challenge than groups A and B (P < 0.05). As regards lesion depth, under polarized light microscopy, group A presented significantly lower values, and groups C and D the highest values. Group B showed a higher value than group A (P < 0.05). Groups A and B exhibited subsurface caries lesions after all treatment durations, while groups C and D presented erosion‐type lesions with surface softening. The model using S. mutans, whether or not it was associated with L. acidophilus, was less aggressive and may be used for the induction of non‐cavitated enamel caries‐like lesions. The optimal period for inducing caries‐like lesions was 8 days. Microsc. Res. Tech. 78:444–451, 2015. © 2015 Wiley Periodicals, Inc.     

External stimulus‐responsive interfaces in polymer nanocomposites

Materials that can switch from stiff to soft or from brittle to tough by slightly changing the temperature, pH, electric, or magnetic fields of their environments, can have a pronounced impact on many applications, including sensors, biomedical materials for less invasive surgeries, and packaging materials. In this work, temperature‐sensitive poly(N‐isopropyl acrylamide) grafts and low molar mass polyethylene glycol were incorporated in the interfacial region between clay nanoparticles and a bacteria‐derived polyhydroxybutyrate matrix to determine if the application of an external stimulus, such as small changes in temperature, could increase the toughness of the nanocomposites. The obtained materials were characterized by using thermal analysis, infrared spectroscopy, dynamic light scattering and dynamic mechanical tests. Mechanical properties were also evaluated at different temperatures and showed that small changes in temperature, such as from 25°C to 40°C, led to a pronounced increase in strain at break of the nanocomposites containing the modified interface. The external stimulus associated with the increase in temperature from 25°C to 40°C can reduce the interfacial interactions between clay and polymer matrix and create a highly plasticized polymer layer between the components of the nanocomposite, which favored the sliding of the interface and ultimately led to high values of elongation. POLYM. COMPOS., 37:1342–1349, 2016. © 2014 Society of Plastics Engineers     

Nutritional Value of Seven Freshwater Fish Species From the Brazilian Pantanal

This study determined the proximate composition and fatty acid profile of the lipid fraction in muscle tissue (fillet) of seven fish species from the Miranda River, Brazil. Total lipid content had the largest coefficient of variation among species (73%), while protein content had the smallest (4.5%), allowing Pimelodus argenteus (mandi-prateado) to be categorized as lean fish; Pimelodus maculatus (mandi-amarelo), Hemisorubim platyrhynchos (jurupoca), and Pinirampus pirinampu (barbado) as species with medium fat content, and Paulicea luetkeni (jaú) and Surubim lima (jurupensém) as fatty fish. In all the species investigated, palmitic acid (23.76–25.99%) was the predominant saturated fatty acid. Oleic acid (16.09–32.90%) was the most abundant monounsaturated fatty acid. Total omega-6 polyunsaturated fatty acids (5.99–15.56%) were the predominant polyunsaturated fatty acids, except in Ageneiosus brevifilis (palmito), in which total omega-3 polyunsaturated fatty acids predominated (10.30%). All the species had favorable indices of nutritional quality for total lipids, with respect to human consumption.     

Identification of temperature and moisture content fields using a combined neural network and clustering method approach

Studies on the dynamics of temperature and moisture content distributions in porous soils have provided important insight on their effect on the building hygrothermal behavior, where the interaction between both building and soil can contribute to reduce building thermal gains or looses. Hygrothermal aspects can be related to many attributes such as energy consumption, occupants' thermal comfort and health, and material deterioration. Recently, a great variety of mathematical models to predict thermal and moisture content profiles in porous media have been presented in the literature. Most of those models are based on analysis of multilayer measurements or on Fourier analysis. The development and validation of such mathematical models facilitate the understanding of heat and moisture flows at different soil depths. In this research, a radial basis function neural network (RBF-NN) approach, combined with Gath–Geva clustering method in order to predict the temperature and moisture content profiles in soils, has been presented. A set of data obtained from the computation of the coupled heat and moisture transfer in porous soils for the Curitiba city (Paraná State, Brazil) weather data file has been used by the RBF-NN modeling method. Simulation results indicate the potentialities of the RBF-NNs to learn, for the one step ahead identification, the behavior of temperature and moisture content profiles in the media at various depths.     

Effect of different surface processing protocols in three-dimensional images for rapid prototyping

The aim of the study was to evaluate the accuracy of virtual three-dimensional reconstructions of human dry mandibles, produced with different surface processing protocols. Three-dimensional images were built from computed tomography scans of 10 dry mandibles, and the surface was smoothed, refined, or both, generating 30 different images. Linear measurements from anatomical landmarks were calculated and compared with the corresponding measurements of the original dry mandible (gold standard). The results showed no differences between the models that were just refined or just smoothed (p > 0.05), when compared to the gold standard. When these two tools were applied together, there was a statistically significant difference (p < 0.01). In conclusion, the application of a single processing tool (smoothing or refinement) in the virtual models does not affect the anatomical measures. However, the simultaneous application of both tools increases the differences between the reconstructions and the original anatomical parts.     

Linear and branched polyoxide‐based copolymers: Methods to determine the CMC

Evaluation of the physical–chemical properties of aqueous solutions of nonionic surfactants based on polyoxides can be performed by different methods. Depending on the technique used, there can be a significant variation in the critical micelle concentration (CMC) found. This is related to the sensitivity of the technique regarding the unimers and micelles present in the solution as well as the structure of the surfactant evaluated. In this work, the CMC values of aqueous solutions of linear and branched poly(ethylene oxide‐polypropylene oxide) (PEO‐PPO) block copolymers were determined by tensiometry, fluorescence, and particle size analysis, using copolymers having adjacent structures (that is, hydrophilic and hydrophobic segments located adjacently in the copolymer) and alternating structures. Tensiometry was used to measure the surface tension as a function of the copolymer concentration in aqueous solution. Fluorescence was used to determine the fluorescence intensity of pyrene to plot the graphs of the I₁/I₃ and I_E/I_M relations according to the surfactant concentration. Finally, particle size analysis was used to determine the diffusion coefficient of the particles. The results showed that the fluorescence and particle size techniques provide lower (and mutually concordant) CMC values and can be considered more precise because these methods directly analyze the bulk of the solution. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009