Mercury distribution in two Sierran forest and one desert sagebrush steppe ecosystems and the effects of fire

Mercury (Hg) concentration, reservoir mass, and Hg reservoir size were determined for vegetation components, litter, and mineral soil for two Sierran forest sites and one desert sagebrush steppe site. Mercury was found to be held primarily in the mineral soil (maximum depth of 60 to 100 cm), which contained more than 90% of the total ecosystem reservoir. However, Hg in foliage, bark, and litter plays a more dominant role in Hg cycling than the mineral soil. Mercury partitioning into ecosystem components at the Sierran forest sites was similar to that observed for other US forest sites. Vegetation and litter Hg reservoirs were significantly smaller in the sagebrush steppe system because of lower biomass. Data collected from these ecosystems after wildfire and prescribed burns showed a significant decrease in the Hg pool from certain reservoirs. No loss from mineral soil was observed for the study areas but data from fire severity points suggested that Hg in the upper few millimeters of surface soil may be volatilized due to exposure to elevated temperatures. Comparison of data from burned and unburned plots suggested that the only significant source of atmospheric Hg from the prescribed burn was combustion of litter. Differences in unburned versus burned Hg reservoirs at the forest wildfire site demonstrated that drastic reduction in the litter and above ground live biomass Hg reservoirs after burning had occurred. Sagebrush and litter were absent in the burned plots after a wildfire suggesting that both reservoirs were released during the fire. Mercury emissions due to fire from the forest prescribed burn, forest wildfire, and sagebrush steppe wildfire sites were roughly estimated at 2.0 to 5.1, 2.2 to 4.9, and 0.36+/-0.13 g ha(-1), respectively, with litter and vegetation being the most important sources.     

Flow separation in a diverging conical duct: Effect of Reynolds number and divergence angle

Fluid flows in passages whose cross-sectional area increases in the streamwise direction are prone to separation. Here, the flow in a conical diffuser fed by a fully developed velocity at its inlet and mated at its downstream end to a long circular pipe is investigated by means of numerical simulation. A universal flow-regime model was used to accommodate possible laminarization of flows having moderate-turbulent and transitional Reynolds numbers at the diffuser inlet. It was found that flow separation occurred for a diffuser expansion angle of 5° for inlet Reynolds numbers less than about 2000. This finding invalidates a prior rule-of-thumb that flow separation first occurs at a divergence angle of seven degrees. Results from the 10 and 30° simulations showed separation at all investigated Reynolds numbers. The largest streamwise length of the separation zones occurred at the lower Reynolds numbers.     

INFORMATION HIGHWAY AND NUMERICAL HEAT TRANSFER

Abstract

It is proposed that researchers in the numerical heat transfer community need to realize the trend of the information highway and agree to use a protocol or a module that constitutes the core of a computer program solving heat transfer problems. This will avoid duplicate programming and accelerate the technology advancement of numerical heat transfer. The module for two-dimensional incompressible Navier-Stokes flows is presented and explained. It is further demonstrated that, using this module as the foundation, the user can straightforwardly build up an entire personal computer code by inputting the data, specifying boundary conditions, and outputting the result. Other modules for slightly more compicated problems, such as transient flows with variable viscosity in irregular geometries, are also presented. Other than zoning matches for problems with multizones, the programming task for a user becomes minimal and simple: input, prescribe the boundary conditions, and output. The availability of Navier-Stokes modules is particularly helpful for less experienced numerical researchers, newcomers, and graduate students who have just entered the area of heat transfer and fluid flows.     

Personalized Development of Antisense Oligonucleotides for Exon Skipping Restores Type XVII Collagen Expression in Junctional Epidermolysis Bullosa

Intermediate junctional epidermolysis bullosa caused by mutations in the COL17A1 gene is characterized by the frequent development of blisters and erosions on the skin and mucous membranes. The rarity of the disease and the heterogeneity of the underlying mutations renders therapy developments challenging. However, the high number of short in-frame exons facilitates the use of antisense oligonucleotides (AON) to restore collagen 17 (C17) expression by inducing exon skipping. In a personalized approach, we designed and tested three AONs in combination with a cationic liposomal carrier for their ability to induce skipping of COL17A1 exon 7 in 2D culture and in 3D skin equivalents. We show that AON-induced exon skipping excludes the targeted exon from pre-mRNA processing, which restores the reading frame, leading to the expression of a slightly truncated protein. Furthermore, the expression and correct deposition of C17 at the dermal–epidermal junction indicates its functionality. Thus, we assume AON-mediated exon skipping to be a promising tool for the treatment of junctional epidermolysis bullosa, particularly applicable in a personalized manner for rare genotypes.