应用电镜技术快速检测传代细胞中的污染因子：—传代细胞污染细菌的快速检测

应用电镜技术检测传代细胞中的细菌污染，即快速又准确。根据细菌的形态及其鞭毛、噬菌体等指标的其中一项均可以判定细菌的污染，该检测结果为传代细胞的应用提供了可靠地保证。     

提高真菌植酸酶热稳定性的方法

植酸酶(Phytase)能提高饲料中磷及其他养分的利用率,减少粪便中磷的排泄量,同时还能降低植酸(盐)的抗营养作用,在饲料中添加植酸酶对提高畜禽生产性能及减轻环境污染有着重要意义。真菌植酸酶具有pH作用范围广、活性高和易提纯等优点,是生产植酸酶的最佳来源。但是真菌植酸酶热稳定性普遍较低,不能完全满足饲料加工的要求。本文综述了在提高真菌植酸酶热稳定性研究方面的最新进展,讨论了其进一步的研究方向。     

PRRSV弱毒细胞疫苗与灭活疫苗生殖道黏膜免疫效果观察

应用组织学方法比较观察了猪繁殖与呼吸综合征病毒(PRRSV)弱毒细胞疫苗与灭活疫苗生殖道黏膜免疫的效果。试验选用5月龄长白×大约克夏二元杂交母猪18头。PRRSV弱毒细胞疫苗和灭活疫苗通过生殖道黏膜免疫接种。结果表明PRRSV弱毒细胞疫苗生殖道黏膜接种组母猪的子宫黏膜上皮内淋巴细胞数较灭活疫苗生殖道黏膜免疫接种组和对照组母猪显著减少(P<0.01);而且子宫黏膜上皮变矮变薄,变得有些不规则。灭活疫苗生殖道黏膜接种组母猪子宫黏膜上皮内淋巴细胞数与对照组母猪间差异不显著(P>0.05)。本试验结果显示PRRSV弱毒细胞疫苗经生殖道黏膜免疫接种则对生殖道黏膜上皮内淋巴细胞产生抑制作用。说明PRRSV弱毒细胞疫苗和灭活疫苗经生殖道黏膜免疫效果不理想     

提高半湿润地区杂交玉米种子发芽率的有效措施

清水县位于甘肃省东南部,介于34°32′～34°58′N、105°45′～106°30′E之间,属陇中南部半湿润区,年降水量662mm左右,降水主要集中在7～9月份,占年降水量的55%.清水县城关川区中晚成熟玉米品种的生长期是从4月下旬后期到9月下旬后期,大致148d左右.     

几种代表性牧草对水牛泌乳高峰期生产性能的影响

将36头泌乳期、产奶量相近的水牛均分3组,探讨禾本科黑麦草、豆科紫花苜蓿、蓼科鲁梅克斯K-1三种牧草对泌乳高峰期生产性能的影响,结果发现,饲喂鲁梅克斯K-1分别比紫花苜蓿和黑麦草提高15.93%、13.14%的产奶量(P<0.05),以及6.09%、6.63%的乳脂率(P<0.01);而紫花苜蓿与黑麦草间差异均不显著(P>0.05)。故种植鲁梅克斯K-1适合我国农村散养水牛的小区放牧或舍饲补饲。     

大型原油储罐的有限元强度分析

采用有限元分析方法对12.5×104m3大型外浮顶式原油储罐在水压试验时的工况进行了分析,得到了储罐各部位的应力分布.借鉴压力容器分析设计标准进行了应力评定,结果表明,储罐在最高液位下可以安全运行.     

Development of Chinese Near-isogenic Line of Rice and Their Differentiating Ability of Pathogenic Races of Blast Fungus

The rice( Oryza L. ) variety, Lijiangxintuanheigu(LTH), is a universally susceptible variety. A set of near-isogenic lines (NILs) of rice which has single resistance gene for each line was developed by use of LTH as recurrent parent and Kusabue,Tsuyuake, K1, PiNo. 4, K60 and BL1, which are from Kiyosawa‘s differential varieties(KDV), as donor parents. Differentiating ability of these NILs to Philippine isolates was compared with that of their donor parents, KDV and IRRI‘ s NILs. The results of comparative studies were as follows: (1) Differentiating ability of Chinese NILs to Philippine isolates was much stronger than that of their donor parents and that of KDV. (2) Chinese NILs had similar ability to differentiate races to IRRI‘s NILs or higher differentiating ability than them. But IRRI‘s NILs almost had no differentiating ability to isolates from japonica rice-growing regions. They can not be used to differentiate races of isolates from japonica ricegrowing regions. Whereas, Chinese NILs can be used to differentiate races of isolates from both japonica ricegrowing regions and indica rice-growing regions. It was concluded that the set of Chinese NILs can be used as international differentials.     

永丰草地的类型及其生产力评估

本文描述了甘肃省天祝县永丰草地的主要草地类型、生产能力及利用模式。研究工作显示,草地改良措施,对不同植被的生物量分布,将产生显著的效果。灌溉和施肥,将促进河谷草甸的地下生物量向地上生物量的转化过程。灌溉可使河谷草甸中禾草组分的增加超过50％,使产草量增加50％～100％。上年秋天和当年春天土壤的含水量,是全年产草量的决定因素,其对产草量的重要性超过全年平均降水量的影响。除了植被组分、产草量的变化外,河谷草地在可消化粗蛋白、可消化能、代谢能等方面也有重大变化,9月份与4月份相比,这三项指标分别高出376％、86％和86％。不同年龄组的绵羊饲料消耗显示,7岁羊的饲料消耗是10月龄羊的17倍;但是7岁羊的胴体重量仅是10月龄羊的2倍。根据永丰滩草地、饲草、家畜的这些特点,当地的畜牧业,不应当在毫无经济意义的情况下,保持太多的牲畜头数,优化畜产品生产的最好方法,就是实行季节畜牧业。季节畜牧业(其中繁殖母羊数达67％)与其他草地改良措施相结合,将使该地区单位面积上的绵羊的产肉量提高4.9倍。     

Cloning of plasma membrane H-ATPase gene in Populus euphratica Oliv.

For this paper, the plasma membrane (PM) H -ATPase gene has been cloned from Populus euphratica Oliv. through a homology based strategy. The isolated 3,210 bp cDNA contains a single 2,862 bp open reading frame (ORF) which encodes a putative H -ATPase protein of 953 amino acid residues, with a significant homology to plasma membrane H -ATPase of Primus persica, Phaseolus vulgaris, Sesbania rostrata and Daucus carota. The predicted protein has a molecular weight of 104,553 Da. The copy number analysis revealed multiple copies of the PM H -ATPase in the P. euphratica genome after digestion of their genomic DNA by the restriction enzymes EcoRI, NdeⅠ, FbaⅠand BglⅡ, and Southern blot.     

鹰咀豆品比研究

本文从世界热带半干旱作物研究中心“ＣＩＣＲＩＳＡＴ”引入鹰咀豆（ＣｉｃｅｒａｒｉｔｉｎｕｍＬ．）品种材料１１２份，在引种筛选的基础上筛选出１７个优秀品种材料，进一步作了品比试验，得到：Ａ３、Ａ２１、Ｂ５、Ｂ７、Ｂ１０、Ｃ１、Ｄ４、Ｄ５８个引入品种。它们抗性强，植株发育生长良好，分枝多，结英率高，种子在甘肃河西走廊可完全成熟，百粒重、籽实产量等均显著地优于当地品种。可在甘肃部分干旱半干旱地区示范性推广，丰富种质资源。