Influence of ADRB1, ADRB2, and COMT Genetic Polymorphisms on Postoperative Outcomes of Patients Undergoing Cardiac Valve Surgery

PurposeThe aim of this study is to prospectively investigate the influence of ADRB and COMT gene polymorphisms on postoperative outcomes of patients undergoing cardiac surgery.MethodsThis prospective cohort study included 223 patients undergoing elective cardiac valve surgery using cardiopulmonary bypass. Demographic information, intraoperative data, postoperative data, and blood samples were collected. Patients were genotyped for single-nucleotide polymorphisms (SNPs) of ADRB1 rs1801253, ADRB2 rs1042713, and COMT rs4680. Major adverse cardiovascular and cerebrovascular events (MACCEs) were used as the primary outcome to evaluate the postoperative prognosis of patients. Secondary outcomes included the duration of mechanical ventilatory support, intensive care unit stay, postoperative hospital stay, and postoperative need of inotropic or vasoactive agents.FindingsThe overall incidence of MACCEs was 15.2%. Among 3 SNP loci, only different genotyped carriers of ADRB2 rs1042713 had statistically significant differences in the incidence of MACCEs (P = 0.005), especially for acute kidney injury (P = 0.023). The proportions of postoperative norepinephrine demand of patients carrying the AA genotype of ADRB2 rs1042713 (P = 0.016) and the AG genotype of COMT rs4680 (P = 0.018) were low. The duration of mechanical ventilatory support (P = 0.034) and postoperative hospital stay (P = 0.045) of patients carrying the AG genotype of COMT rs4680 was shortest. After multiple logistic regression analysis, we found that the G allele carriers of ADRB2 rs1042713 had a higher risk of MACCEs (AG vs AA genotype: odds ratio [OR] = 4.348; 95% CI, 1.529–12.359, P = 0.006; GG vs AA genotype: OR = 3.722; 95% CI, 1.060–13.071; P = 0.040), in particular with acute kidney injury (AG vs AA genotype: OR = 5.273; 95% CI, 1.093–25.451; P = 0.038; GG vs AA genotype: OR = 7.533; 95% CI, 1.275–44.522; P = 0.026). There was no SNP-SNP interaction found among the 3 SNPs with multifactor dimensionality reduction analysis.ImplicationThe ADRB2 rs1042713 polymorphism might be related to prognosis of patients undergoing cardiac surgery. Patients carrying the G allele of ADRB2 rs1042713 had a higher risk of developing MACCEs, especially acute kidney injury. chictr.org.com identifier: ChiCTR1800015105.     

Effect of Scraping Therapy on Chronic Low Back Pain: A Systematic Review and Meta-analysis of Randomized Controlled Trials

ObjectiveThe purpose of this study was to review the literature on the effect of scraping therapy on chronic low back pain (LBP) from randomized controlled trials (RCTs).MethodsThree English medical electronic databases (PubMed, Embase, and the Cochrane Library) and 2 Chinese databases (China National Knowledge Infrastructure and Wanfang) were searched. Only randomized controlled trials related to the effects of scraping therapy on chronic LBP were included in this systematic review. Study selection, data extraction, and validation were conducted independently by 2 reviewers. The methodological quality of the studies was evaluated by the Cochrane risk-of-bias tool. RevMan 5.3 software was applied to perform meta-analysis of the data.ResultsTen studies comprising 627 participants were included. Overall, the quality of evidence was moderate owing to a lack of blinding and allocation concealment in some studies and unclear risk of selective reporting. Meta-analysis of 9 RCTs indicated that scraping therapy had a statistically significant effect on pain reduction (standard mean difference = ?0.66, 95% confidence interval [CI], ?0.83 to ?0.49, P < .001). However, if only a single scrape treatment was carried out, the results did not show that scraping was superior to the control group regarding pain relief (mean difference = ?0.35, 95% CI, ?1.23 to 0.53, P = .44). Moreover, the results of 6 RCTs involving 468 participants showed significantly greater improvement in lumbar dysfunction (mean difference = ?10.05, 95% CI, ?13.52 to ?2.32, P < .001). In addition, the results of 5 RCTs involving 393 participants showed a favorably significant effect on the overall efficacy (odds ratio = 4.74, 95% CI, 2.34-9.62, P < .001). As for follow-up effects, meta-analysis of 3 RCTs involving 241 participants showed a promising effect on pain reduction and lumbar function improvement at 1 month and 3 months after the end of treatment, respectively. Only 1 study reported adverse effects, and none were serious.ConclusionScraping therapy may have a therapeutic effect for some individuals with chronic LBP. However, due to the limited amount of research and the low methodological quality of the included studies, additional large-scale, multicenter, high-quality RCTs on relieving pain intensity and improving lumbar dysfunction are still necessary.     

不稳定血红蛋白病Hb Alesha一例报告并文献复习

不稳定血红蛋白病(unstable hemoglobinopathy,UHb)是一类由于珠蛋白结构异常引起血红蛋白不稳定的疾病。90%以上的结构异常是由于编码珠蛋白基因单个核苷酸替代导致的错义突变,引起相应氨基酸的改变,使血红蛋白不稳定,红细胞易于被破坏。目前全球报道了150余种UHb[1],大部分属常染色体显性遗传,但也有不少患者无家族史。本文报道不稳定血红蛋白病Hb Alesha 1例并总结复习相关文献,以提高对该病的认识。     

1例剖宫产术后阵发性睡眠性血红蛋白尿症患者继发脾破裂的护理

总结1例剖宫产术后阵发性睡眠性血红蛋白尿症患者继发脾破裂的护理经验。包括脾破裂后快速建立静脉通路,密切监测血压、腹内压变化;减少输血时的溶血反应并做好栓塞的观察与预防;血浆置换的管理;依库丽单抗注射液的应用,避免不良反应的发生;进行保护性隔离,降低感染风险;做好患者的心理护理及健康宣教,帮助其树立战胜疾病的信心。经过精心的治疗及护理,患者于入院后16 d出院。     

A large congenital atrial septal defect in an adult with delayed therapy

Patients with a large congenital atrial septal defect (ASD) traditionally have the ASD repaired at the preschool age. Unfortunately, insufficient education of patients regarding medical science and clinical recommendations can lead to delayed therapy, resulting in complications during adulthood. We report a rare case of a large congenital ASD in a 20-year-old man. Echocardiography showed a 67-mm ostium secundum defect and moderate mitral and tricuspid regurgitation. The patient underwent transthoracic ASD repair along with mitral and tricuspid valvuloplasty. This report emphasizes the importance of educating patients about congenital malformations and potential interventions in developing countries, particularly in rural communities.     

2015－2019年中国居民心脑血管事件报告数据质量分析

  目的  分析2015 — 2019年中国居民心脑血管事件报告数据质量。  方法  选取河北、江苏、浙江、湖北和四川5个省10个国家心脑血管事件报告监测点,抽取2015 — 2019年其辖区内1家二级及以上医疗机构报告的“脑卒中急性事件报告卡”和“急性心肌梗死事件报告卡”（来源于中国疾病预防控制中心“全民健康保障信息化疾病预防控制信息系统”）若干份,利用中国疾病预防控制中心慢性非传染性疾病预防控制中心开发的“心脑血管事件质量研究信息系统”,由相关人员采集相应的临床资料,复核判定报告卡填写的完整性和准确性。  结果  查找并复核急性脑卒中事件报告卡2 184份核心变量报告完整率为100.00%,核心变量报告准确率为79.21%～95.15%,经复核有2 062份是脑卒中急性发作事件,阳性预测值为94.41%（93.07%～96.14%）。 2015 — 2019年脑卒中核心变量报告准确性呈现增高趋势（P<0.001）。 查找并复核急性心肌梗死报告卡699份,核心变量报告完整率为100.00%,核心变量报告准确率为75.10%～95.42%,经复核有668份为急性心肌梗死事件,阳性预测值为95.57%（92.86%～97.45%）。 2015 — 2019年急性心肌梗死报告卡诊断依据完整性和核心变量准确性呈现增长趋势（均P<0.001）。  结论  2015 — 2019年心脑血管事件报告卡完整性较高,报告准确性逐渐提升,仍然存在着一定比例的错报,需要进一步加强报告规范性。     

Alleles of keratin 1 in families and populations

Keratin 1 is found in the upper layers of the epidermis, on the surface of endothelial cells and in the membrane of the neuroblastoma NMB7. It is important for the structural integrity of the skin, has been found to regulate the activity of kinases, such as protein kinase C (PKC) and SRC, to participate in complement activation by the lectin pathway and to be involved in fibrinolysis, angiogenesis and the response to oxidative stress. Studies of the polymorphisms of the Keratin 1 (KRT1) gene have been driven mostly by interest in its role in skin diseases. However, much of the KRT1 variation occurs in normal populations and is not associated with dermal pathology. In the present experiments, we have investigated the polymorphism of KRT1 genes by nucleotide sequencing in normal families and normal populations of European, African, Hispanic and Asian background. The frequencies of the KRT1 alleles were strikingly different in the four ethnic groups and most of the mutations resulted in amino acid substitutions, with only 3 out of 19 being synonymous. Analysis of selective neutrality by the Ewens–Watterson and Tajima D statistics showed that KRT1 allele homozygosity was decreased in three of the populations suggesting that KRT1 genes may be under the influence of balancing selection. It is possible that the role of KRT1 as a receptor, rather than its structural function in the epidermis, is what drives the selective forces that are apparent in the inheritance of this gene.     

Different roles of PD-L1 and FasL in immunomodulation mediated by human placenta-derived mesenchymal stem cells

Mesenchymal stem cells (MSCs) derived from either bone marrow (BMSCs) or placenta (PMSCs) have the capacity to suppress immune responses to mitogenic and allogeneic stimulations. Both cell contact and soluble factor dependent mechanisms have been proposed to explain this immunosuppression. This study explored the roles of some of cell surface molecules expressed on human PMSCs (hPMSCs) in hPMSC mediated immunomodulation. hPMSCs strongly suppressed mitogen and allogeneic peripheral mononuclear cells (PBMCs) induced T cell activation and proliferation. hPMSCs constituently expressed programmed death-ligand 1 (PD-L1) and Fas ligand (FasL) molecules. Neutralising antibodies to-PD-L1 and FasL significantly reduced the suppressive effect of hPMSCs on T cell proliferation. However, only anti-PD-L1 antibody partially restored early T cell activation suppressed by hPMSCs. Anti-FasL antibody but not anti-PD-L1 antibody reduced apoptosis of activated T cell indicating that FasL molecule plays a role in inducing apoptosis of activated T cells, although overall hPMSCs diminished T cell apoptosis. Different effects of PD-L1 and FasL molecules on T cell activation and activated T cell apoptosis suggest that these two molecules influence T cell response at different stages. hPMSCs significantly prevented activated T cells from going into S phase. Both antibodies to PD-L1 and FasL had significant effect on reversing the effect of hPMSCs on cell cycles. hPMSCs reduced INF-γ but increased IL-10 production by mitogen activated T cells. Both antibodies partially abolished the effect of hPMSCs on INF-γ and IL-10 production. These data demonstrated that PD-L1 and FasL molecules play significant roles in immunomodulation mediated by hPMSCs. This study provides a rational basis for modulation of negative costimulators on hPMSCs to increase their immunosuppressive properties in their therapeutic applications.     

Mannose-binding lectin gene polymorphisms are not associated with susceptibility to severe early childhood caries

Our purpose was to investigate a possible relationship between severe early childhood caries (S-ECC) and polymorphism of the mannose binding lectin gene and investigate the role of allele variant as a possible factor in the susceptibility to S-ECC. Sixty-two Chinese children with S-ECC and 68 caries-free control children were included in this study. Genomic DNA was extracted from buccal epithelial cells of each individual. The identification of MBL B allele was performed by restriction fragment length polymorphism using Ban I restriction enzyme. The frequency of MBL mutant genotype (GGC/GAC and GAC/GAC) was more frequent among children with S-ECC compared with control groups, but did not significantly differ between two groups (x² = 2.82, p > 0.05). There was no significant difference in the allele frequency of codon54 wild type (allele A) between two groups (x² = 2.76, p > 0.05). The present study did not find evidence of MBL codon54 polymorphisms being associated with S-ECC in the population studied, but a larger sample size is necessary to confirm the present results.