振动对急性缺氧小鼠肺损伤的影响

本文观察了频率为20Hz、振幅为0．67mm和作用时间为40min的振动预处理对急性缺氧小鼠肺损伤的影响。结果发现实验组的肺组织匀浆脂质过氧化物LghdPeroxde（LPO）、支气管肺泡灌洗液（BALF）中蛋白含量和白细胞数明显低于对照组（P＜0．05）。这表明在特定率数的振动作用下能明显减轻急性缺氧小鼠肺的损伤。     

Long-term stability of prism correction of heterophorics and heterotropics; a 5 year follow-up. Part I: Heterophorics

Twenty heterophoric and 10 heterotropic patients with long standing severe visual symptoms were corrected with prisms for permanent wearing using the full-correction method of H.-J. Haase. The patients were reexamined 1 and 5 years after completion of treatment. Stable corrections obtained by stepwise optical full-correction were also maintained during a follow-up period of 5 years. Sensory and motor adaptations were largely removed and asymptomatic binocular vision was obtained for nearly all patients. A few cases of partly irreversible amblyopia were indicated, but even in these cases largely normal binocular functions were attained. When glasses were suspended, sensory and motor readaptations took place.     

EFFECTS OF STROPHANTHIDIN ON THE SLOW INWARD CURRENT IN GUINEA-PIG ISOLATED VENTRICULAR MYOCYTES

1. The effect of strophanthidin on the slow inward current (Isi) and on contractile force were studied in guinea-pig isolated ventricular myocytes and intact papillary muscles, respectively. In myocytes, both low (10 nmol/L) and high (1-10 mumols/L) concentrations had small or no effects in either direction on Isi whereas norepinephrine (10-100 nmol/L) increased it. To determine whether the same results are obtained after decreasing or increasing intracellular calcium or sodium, the same concentrations of strophanthidin were tested in different procedures that are known to (i) increase [Ca]i and decrease [Na]i (high [Ca]o, 3.6-5.4 mmol/L; low [Na]o, 112 mmol/L; (ii) decrease [Ca]i and increase [Na]i (low [Ca]o, 0.45-1 mmol/L; Sr, 1 mmol/L; (iii) decrease [Ca]i and [Na]i (Cd, 0.1-0.2 mmol/L); and (iv) increase [Ca]i and [Na]i (veratridine, 0.2 mumol/L). High [Ca]o and veratridine increased whereas low [Ca]o and Cd decreased Isi. In contrast, during these various procedures, strophanthidin had small and inconsistent effects at a low or high concentration. In intact papillary muscles, low strophanthidin decreased whereas high strophanthidin increased contractile force. It is concluded that strophanthidin has little direct or indirect effect on Isi and that the decrease in force by low and increase in force by high concentrations in intact muscle are probably related to demonstrated decrease and increase, respectively, in intracellular sodium activity.     

大鼠腹腔巨噬细胞表达促肾上腺皮质激素释放激素的实验研究

目的 观察促肾上腺皮质激素释放激素(CRH)在大鼠腹腔巨噬细胞的表达变化规律.方法 体外培养大鼠腹腔巨噬细胞,采用RT-PCR及ELISA技术检测正常及脂多糖(LPS)、佛波脂(PMA)刺激后,细胞CRH mRNA转录水平及CRH多肽合成水平.结果 RT-PCR结果显示,正常大鼠腹腔巨噬细胞内有一定水平的CRH mRNA,LPS及PMA刺激后,细胞内CRH mRNA水平均显著增高(P＜0.01).在多肽水平,正常大鼠腹腔巨噬细胞可表达低水平的CRH[约(0.07±0.01)ng/107cells],LPS及PMA促进了CRH多肽的合成(P＜0.01).结论 正常大鼠腹腔巨噬细胞可合成表达CRH,其水平在活化后大鼠腹腔巨噬细胞显著上调.     

岛津数字胃肠机IF OVER故障产生的原理及维修

分析了岛津3200数字胃肠机IFOVER故障产生的原因，并结合实际故障现象，具体介绍了排除方法。     

直肠癌全直肠系膜切除术后直肠阴道瘘原因与防治

本文通过分析近5年来我院18例女性直肠癌全直肠系膜切除术后直肠阴道痿的临床资料。发现直肠阴道痿多发生于中低位直肠癌行全直肠系膜切除保肛手术的患者。痿的发生与肿瘤的位置、肿瘤的分期、肿瘤距肛门口的距离．以及手术技巧、手术难度、引流方法有关。而与是否进行预防性造口，以及是否采用腹腔镜手术无关。预防上强调应重视术中直肠前壁的锐性分离、结肠直肠吻合以及术后盆腔的负压引流。治疗上应首先进行保守治疗，即肠内营养，阴道冲洗。早期配合肠外营养、及全身或局部抗炎治疗，大部分直肠阴道痿通过保守治疗可以治愈。保守治疗无效时考虑结肠或回肠末端造口。若长期不愈应考虑吻合口肿瘤复发的可能。     

表皮生长因子影响肿瘤患者舌苔变化的分子机制研究

目的 研究表皮生长因子（EGF）对食管癌细胞Eca-109细胞周期及表皮生长因子受体（EGF－R）表达的影响，探讨EGF促进肿瘤患者舌苔增厚的分子机制。方法 应用流式细胞术，检测EGF－R的表达和细胞周期。结果 EGF能明显促进Eca-109细胞膜上EGF－R的表达，使细胞增殖活性大大增强。结论EGF有是通过促进EGF－R的表达影响舌苔形成。     

Increased amplification proximal to the MLL gene in acute myeloid leukemia

Amplification of the chromosomal region 11q23 encompassing the MLL gene has been observed in patients with acute myeloid leukemia (AML). Patients with this abnormality often have a poor response to chemotherapy and short survival. We have studied the regions flanking the MLL gene using 8 bacterial artificial chromosome (BAC) probes and dual color fluorescence in situ hybridization (FISH) analysis. Two contigs of BAC probes flanking the MLL gene, were constructed by standard primer walking and BAC end sequencing. For comparison, metaphase chromosome preparations were also hybridized with a commercial MLL specific probe. Metaphase chromosomes were prepared from the bone marrow sample of an 80-year old female patient with AML-M1 and the cytogenetic aberration der(11)hsr(11) (q23). FISH analysis on metaphase chromosomes showed amplification on the homogeneously staining region (hsr) and marker chromosomes for both the MLL gene and the BAC probes. Using dual color FISH, probes proximal to MLL showed greater amplification than those distal to MLL, as represented by additional red signals on both metaphase and interphase chromosomes. Ratios of the copy numbers of the BAC probes relative to the chromosome 11 centromere copy number confirmed a higher copy number for probes proximal to MLL. These results suggest that other gene(s) proximal to MLL could be the target gene(s) of amplification in this case and not the MLL gene as previously assumed.     

胶原基真皮再生支架的微结构控制

综述了影响胶原基真皮再生支架微结构的各种因素及其控制方法。胶原基真皮支架的生物活性和修复创面的能力受到诸如除胶原外的其它主要材料 (第二组分 )、支架的孔径和孔隙率、支架厚度、生物活性因子以及交联度等多方面因素的综合影响。从材料学、生物学和医学的角度综合地应用物理、化学和生物学的手段探索各种影响因素的控制方法是组织工程皮肤研究的重点。     

肝素化胶原/壳聚糖多孔支架的制备及其血管化的研究

本研究旨在构建一种能快速血管化的人工真皮替代物。用冻干法制备了胶原／壳聚糖多孔支架，并对其进行肝素化，观察此支架的结构特征、亲水性、体外降解性和组织相容性，同时将血管生成素引入到此支架，对复合有血管生成素的肝素化支架的体内血管化进行了初步研究。结果表明，肝素化胶原／壳聚糖多孔支架具有合适的三维多孔结构、良好的吸水性和较理想的酶解稳定性，体内实验表明，此支架具有良好的组织相容性，血管生成素可加快支架的血管化。