阿苯达唑联合黄芪对华支睾吸虫感染大鼠肝功能异常的疗效观察

Objective To observe the levels of alanine aminotransferase(ALT), total bilirubin(TBIL), hyaluronic acid (HA), procollagen type Ⅲ aminoterminal peptide (P Ⅲ NP) and larninin (LN) in the sera of rats infected with Clonorchis sinensis (C. sinensis) after treatment of albendazole combined with milkvetch root. Methods Thirty-two healthy adult Wistar rats were randomly divided into four groups with 8 in each based on body mass: control group, non-treatment group, Albendazole group(ALB group) and albendazole combined with milkvetch root group(ALB+MR group). The rats in non-treatmen, ALB and ALB+MR groups were infected orally with metacercariae of C. sinensis 50 per rat. The rats in control group were mock-infected with saline. The rats in ALB group were treated to each rat with 50 mg/kg alhendazole for 5 days, and ALB+MR groups were given to same treat with albendazole, meanwhile each rat injected with 800 mg/kg milkvetch root intraperitoneally for 30 days. All rats were killed after infestation 14 weeks and their sera samples were collected to detect ALT, TBIL, HA, PⅢNP, LN. Results There were statistically significant differences in the levels of ALT and LN in the sera of rats between groups(F=31.40,11.82, P<0.01). Compared with control[ (47.88±4.88)U/L, (51.20±4.12)μg/L], the levels of ALT and LN in rats in non-treatment group [(85.50±9.65)U/L, (64.20±4.18) μg/L] and ALB group [(65.29± 7.78) U/L, (58.23±2.55) μg/L] were significantly increased (P<0.05). Compared with non-treatment group, the levels of ALT and LN in rats in ALB group and ALB+MR groups[(50.25±9.29)U/L, (53.68±5.63)μg/L] were significantly decreased(P<0.05), and they decreased more obviously in ALB+MR group (P<0.05). There were statistically significant differences in the levels of TBIL, PⅢNP and HA in the sera of rats between groups (χ2=15.309,21.418,19.759, P<0.01). Compared with control[(0.700±0.350)μmol/L, (26.085±4.075)μg/L, (81.935±42.550)μg/L], the levels of TBIL, PⅢNP and HA in rats in non-treatment group(2.400 μmol/L, 46.220 μg/L,310.885 μg/L) and ALT group(1.200 μmol/L,36.540 μg/L, 178.010 μg/L) were significantly increased(P<0.05). Compared with non-treatment group, the level of TBIL in ALT+MR group(0.750 μmol/L), the levels of pⅢNP and HA in ALT and ALT+MR group(30.470,100.240 μg/L) were significantly decreased(P< 0.05). The levels of TBIL, PⅢNP and HA decreased more obviously in ALB+MR group(P<0.05). Conclusions The liver function in rats infected with C. sinensis is abnormal. The liver function and fibrosis are improved after treatment with albendazole or albendazole combined with milkvetch root. The treatment of albendazole combined with milkvetch root is more effective.     

大柴胡汤对血清脂质代谢及颈总动脉血流动态的影响

过去的报告认为大柴胡汤提取物颗粒剂对高脂血症患者的血清脂质有改善作用,但对脑及脏器循环的影响研究尚少,因而研究了大柴胡汤对高脂血症患者的血清脂质代谢及颈总动脉血流动态的影响。对象为不合并     

阿苯达唑联合黄芪对华支睾吸虫感染大鼠肝功能异常的疗效观察

目的 观察阿苯达唑联合黄芪对华支睾吸虫感染大鼠肝功能异常的疗效.方法 健康成年Wistar大鼠32只,按体质量随机分为对照组、未治疗组、阿苯达唑组和阿苯达唑+黄芪组.每组8只.未治疗组、阿苯达唑组和阿苯达唑+黄芪组大鼠给华支睾吸虫囊蚴50个/只灌胃,对照组大鼠给生理盐水灌胃:阿苯达唑组大鼠在感染后10周每日每只给阿苯达唑50 mg/kg灌胃,连续5 d;阿苯达唑+黄芪组大鼠在给阿苯达唑的同时腹腔注射黄芪注射液800 mg/kg,连续30 d.于感染后14周采集血样,检测血清丙氨酸转氨酶(ALT)、总胆红素(TBIL)、透明质酸酶(HA)、层粘连蛋白(LN)和Ⅲ型前胶原(PCⅢP)水平.结果 大鼠血清ALT和LN水平组间比较,差异有统计学意义(F值分别为31.40、11.82,P<0.01).血清ALT和LN水平,未治疗组[(85.50±9.65)U/L、(64.20±4.18)μg/L]、阿苯达唑组[(65.29±7.78)U/L、(58.23±2.55)μg/L]较对照组[(47.88±4.88)U/L、(51.20±4.12)μg/L]明显升高(P<0.05);阿苯达唑组和阿苯达唑+黄芪组[(50.25±9.29)U/L、(53.68±5.63)μg/L]较未治疗组明显下降(P<0.05),阿苯达唑+黄芪组下降较阿苯达唑组更明显(P<0.05).大鼠血清TBIL、PⅢNP和HA组间比较差异有统计学意义(χ2值分别是15.309、21.418、19.759,P<0.01).血清TBIL、PⅢNP、HA水平,未治疗组(2.400 μmol/L、46.220 μg/L、310.885 μg/L)、阿苯达唑组(1.200 μmol/L、36.540 μg/,L、178.010 μg/L)较对照组(0.700 μmol/L、26.085 μg/L、81.935 μg/L)明显升高(P<0.05).大鼠血清TBIL水平,阿苯达唑+黄芪组(0.750 μmol/L)较未治疗组明显降低(P<0.05):大鼠血清PⅢNP和HA水平,阿苯达唑组和阿苯达唑+黄芪组(30.470 μg/L、100.240 μg/L)较未治疗组明显降低(P<0.05),与阿苯达唑组比较,阿苯达唑+黄芪组大鼠血清TBIL、pⅢNP和HA水平下降更明显(P<0.05).结论 大鼠感染华支睾吸虫后肝功能异常,经阿苯达唑联合黄芪治疗后肝功能得到改善,肝纤维化减轻,阿苯达唑联合黄芪治疗效果好于单独应用阿苯达唑治疗.     

哈尔滨一牛源赖氏隐孢子虫分离株的分子鉴定

目的对来源于哈尔滨某奶牛场一头3月龄奶牛的一个隐孢子虫分离株进行了分子鉴定。选择隐孢子虫基因分型常用的18SrRNA和actin基因位点,设计引物,用巢氏PCR方法分别扩增出约840bp和1066bp目的片段。PCR产物经双向测序后用ClustlX、DNAstar和PHLIP软件进行序列分析。结果表明在18SrRNA基因位点上,此分离株与报道的牛源C.ryanae分离株(DQ871345、AY587166、EU410344)同源性最高,达到100%。在actin位点,此分离株和美国牛源C.ryanae分离株(EU410345)和隐孢子虫鹿样基因型(AY741309)同源性也为100%。种系发育分析显示所获得的分离株在两个基因位点上均和C.ryanae处于同一进化分支。这些分析表明此次分离获得的隐孢子虫分离株为C.ryanae。鉴定结果为研究我国牛源隐孢子虫种类分布以及评价其公共卫生意义提供了重要参考。     

毛里培尼亚保盖凯地小学生埃及血吸虫感染调查

埃及血吸虫病在毛里塔尼亚流行比较广泛,对人民健康威胁很大,为了解其感染情况,我们于2002年10月27日至11月7日,在保盖、凯地两地小学生中开展了埃及血吸虫病调查,现报告如下。1材料与方法1.1样本采集毛里塔尼亚保盖、凯地各小学校学生尿液,随机抽样调查850人。1.2方法1.2.1病原学诊断将尿样放入离心管内适量,离心1000~1500r/min,3~5min弃去尿液,取底部沉淀物0.3~0.5ml,涂于载玻片上低倍镜下观察[1],查到埃及血吸虫卵为诊断依据。1.2.2血尿检测将试纸条放入尿样离心管内,检测尿液中是否存在血尿,1min后观察结果[2]。2结果共调查两地小学生85…     

肝吸虫感染大鼠红细胞免疫功能的动态变化

目的:探讨大鼠感染肝吸虫前后红细胞免疫功能的动态变化.方法:取健康成年Wistar大鼠48只,随机分为正常对照组8只和感染组40只,感染组大鼠口服肝吸虫囊蚴建立肝吸虫病动物模型,分别于灌胃后0、2、4、6、8和14周处死.对肝吸虫病大鼠的红细胞C3b受体花环率(RBC-C3bRR),红细胞免疫复合物花环率(RBC-ICR),红细胞粘附促进因子(RFER)及红细胞粘附抑制因子(RFIR)的活性分别进行动态检测.结果:肝吸虫病大鼠RBC-C3bRR和RFER水平降低,而ICRR和RFIR水平升高,与正常对照组比较差异显著,有统计学意义.结论:肝吸虫病大鼠的红细胞免疫功能发生改变,表现为继发性红细胞免疫功能异常和红细胞免疫调节功能紊乱,说明肝吸虫感染可损伤宿主的红细胞免疫功能.检查肝吸虫病大鼠红细胞免疫功能对了解其免疫状态具有一定的意义.     

哈尔滨市在校大学生面部蠕形螨感染调查

人体蠕形螨(human demodex)俗称毛囊虫,为蠕形螨科蠕型螨属,是寄生在人及其他哺乳动物的毛囊和皮脂腺内的一种条件致病性螨类.蠕形螨以皮肤的角质蛋白及细胞代谢产物为食,常侵犯人的颜面部如鼻、前额、颊部等皮脂发达的部位.蠕形螨呈世界性分布,人群普遍易感,国外报道人群感染率为27％～100％,国内报道感染率为0.8％～81％[1].近年来的研究表明,蠕形螨与一般皮肤病如酒渣鼻、痤疮及脂溢性皮炎等有一定关系[2].个人卫生习惯、皮肤类型与蠕形螨感染密切相关[3,4],具有居室聚集性,容易在人群中传播[5].为了了解当地在校大学生蠕形螨感染情况,我们对哈尔滨医科大学在校住宿的大学生进行了抽样调查,结果报告如下.     

细胞凋亡在肝吸虫感染大鼠及肝吸虫病患者肝损伤的作用

目的 分析肝吸虫感染大鼠和肝吸虫病患者肝组织的病理改变及损伤情况,探讨细胞凋亡在肝吸虫感染所致肝损伤中的作用.方法 将Wistar大鼠分为2组:感染组60只,对照组20只.感染组大鼠经口饲感染囊蚴法建立肝吸虫感染模型,对照组大鼠采用生理盐水灌胃.于感染后第4、6、8、12周时处死动物.同时收集尸检获得的肝吸虫病患者及健康对照人体肝组织标本各4例.光镜下观察大鼠和人体肝吸虫感染情况以及肝组织的病理学改变;并用TUNEL法检测肝细胞的凋亡率.结果 光镜下各时间点感染组大鼠和肝吸虫病患者胆管壁增厚,胆管黏膜呈乳头状和腺瘤样增生,管腔内可见虫体及虫卵,胆管周围可见炎细胞浸润和少量纤维组织增生,汇管区周围有一些肝细胞出现核固缩,呈现细胞凋亡的形态特征.感染组大鼠第4、6、8、12周时肝细胞凋亡率分别为(7.15±1.50)%、(11.61±3.09)%、(13.21±3.47)%、(11.26±4.06)%,均明显高于对照组[(2.57±0.72)%、(3.17±0.77)%、(3.67±0.96)%、(2.84±0.87)%,t值分别为4.45、5.49、5.95、4.74,P均<0.01].肝吸虫病患者肝组织细胞凋亡率为(12.02±1.78)%,明显高于对照组[(2.03±0.68)%,t=15.13,P<0.01].结论 肝吸虫感染可以引起宿主肝细胞凋亡,后者可能与感染者肝损伤有关.

Abstract：

Objective To investigate the liver injury and pathological changes of rat and patients with Clonorchis sinensis(C, sinensis) infection, and to clarify the role of apoptosis in the injury induced by C. sinensis.Methods Wistar rats were divided into two group: 60 in infection group and 20 in control. The rats in infection group were infected with C. sinensis via oral feeding encysted cercaria;rats in control group were fed with normal saline. The rats were sacrificed 4, 6, 8 and 12 weeks after infection, respectively. Liver tissue specimens of the patients infected with C. sinensis were collected. The pathological changes of liver tissue were observed by light microscopy and the apoptofic rate of hepatocyte was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL) assay. Results Parasites and eggs could he seen around the bile duct, and the duct was associated with mucosa and adenoma papillary hyperplasia, wall thickening, inflammatory cell infiltration, a small amount of fibrous tissue hyperplasia, and periportal liver cells surrounded by a number of nuclear condensation, all these changes meant morphological characteristics of apoptosis. Apoptotic rates of liver cells in infection group 4, 6,8 and 12 weeks after infection were (7.15 ± 1.50)%,(11.61 ± 3.09)%,(13.21 ± 3.47)% and (11.26 ± 4.06)%,respectively, which was significantly higher than that in control group [(2.57 ± 0.72)%, (3.17 + 0.77)%, (3.67 ±0.96)% and (2.84 ± 0.87)%, t values were 4.45, 5.49, 5.95 and 4.74, respectively, all P < 0.01]. Conclusions These findings indicate that C, sinensis can stimulate both hepatoeytic apoptosis and degeneration which may he related to clinical manifestations and liver lesions in patients with clonorchiasis.