地方病调查中随机数字表的应用

在地方病抽样调查过程中,为了获取有代表性的样本,常采用随机化的方法.在实际工作中若调查总体不大的情况下,常通过随机数字表来实现.作者以吉林省克山病病情调查为例介绍随机抽样中随机数字表的使用方法,为地方病现场调查提供参考.     

克山病病区玉米及酱块对大鼠心肌损伤的实验研究

Objective To investigate the myocardial damage in rats fed with corn from Keshan disease area added with bean puree. Methods Male Wistar rats were randomly divided into 3 groups according to their body weights, and fed with corn, corn from Keshan disease area added with bean puree, corn from non-endemic area. The GSH-Px activity of vena cardalis blood was examined in 1 and 3 months, rats were sacrificed after being fed for 6 months to examine the heart changes with HE stain. Results The three groups of GSH-Px activity were different in 1 and 3 months respectively(F=23.60,72.46, all P<0.01); GSH-Px activity was (181.58±22.15), (44.76±28.59)U/L in rats fed with corn, was (195.03±17.66), (30.38±3.35)U/L in those fed with corn added with bean puree from Keshan disease area, lower than the group fed with corn of non-endemic area[(340.90±95.42), (125.17±13.64)U/L, all P < 0.01]. But the difference of GSH-Px activity between simple corn group and corn adding bean puree groups of Keshan disease area was not obvious(P>0.05). Myocardial damage incidence of the three groups was 3/9,1/9,2/7. Difference among three groups did not have statistical significance(χ2=1.33, P> 0.05). Conclusions Only corn from Keshan disease area may induce myocardial damage pathology change. Adding bean puree into corn does not increase damage.     

地方病调查中随机数字表的应用

在地方病抽样调查过程中,为了获取有代表性的样本,常采用随机化的方法.在实际工作中若调查总体不大的情况下,常通过随机数字表来实现.作者以吉林省克山病病情调查为例介绍随机抽样中随机数字表的使用方法,为地方病现场调查提供参考.     

地方病调查中随机数字表的应用

在地方病抽样调查过程中,为了获取有代表性的样本,常采用随机化的方法.在实际工作中若调查总体不大的情况下,常通过随机数字表来实现.作者以吉林省克山病病情调查为例介绍随机抽样中随机数字表的使用方法,为地方病现场调查提供参考.     

桂枝加龙骨牡蛎汤临床应用举例

桂枝加龙骨杜蛎汤出自《金匮要略·血痹病劳》篇,系由桂枝汤加龙骨、牡蛎等七味药物组成,具有调和营卫,摄阴补阳,安神固涩的作用,仲景用于治疗虚劳病阴阳两虚而致“男子失精,女子梦交”之症。     

针灸治愈冲疝2则

例1:曹×,男,64岁,农民,1985年9月12日就诊。发病前曾患风寒感冒,咳嗽痰多稀白,当天下午因用力咳嗽,左侧腹股沟下突然肿起1包块,其大如蛋,向上攻痛,上至心下,下引睾丸,疼痛难忍,阴囊湿冷,恶心呕吐,面白汗出,手足不温,大便不通,小便困难,舌淡、苔白润,脉弦缓而沉。体查:左侧阴囊肿大如蛋,按之坚硬,压痛明显,上腹部软,肝脾未触及,肠鸣音亢进,右下腹(-)。西医诊断:腹股沟斜疝。中医诊断:冲疝。为阴寒内盛,寒邪从少腹向上冲逆所致。治宜温经散寒,降逆止痛。     

克山病病区玉米及酱块对大鼠心肌损伤的实验研究

目的 探讨克山病病区玉米及酱块与大鼠心肌损伤的关系.方法 将雄性Wistar大鼠按体质量随机分为3组,分别饲以病区玉米、病区玉米+酱块、非病区玉米,1、3个月时检测大鼠尾静脉全血谷胱甘肽过氧化物酶(GSH-Px)活力,6个月时光镜下观察大鼠心肌病理改变.结果 1、3个月时3组大鼠全血GSH-Px活力比较,差异有统计学意义(F=23.60、72.46,P均<0.01),其中病区玉米组[(181.58±22.15)、(44.76±28.59)U/L]和病区玉米+酱块组[(195.03±17.66)、(30.38±3.35)U/L]明显低于非病区玉米组[(340.90±95.42)、(125.17±13.64)U/L,P均<0.01],而病区玉米组与病区玉米+酱块组比较,未见明显改变(P>0.05).光镜下3组大鼠心肌损伤病理改变发生率分别为3/9、1/9、2/7,组间比较差异无统计学意义(P>0.05).结论 单纯克山病病区玉米饲养可引起大鼠心肌损伤病理性改变,酱块无加重心肌损伤作用.     

克山病患者抗氧化能力测定

目的 探讨克山病患者体内抗氧化水平与克山病心肌损伤的关系,分析其可能的发病机制.方法 2005和2006年期间,在黑龙江省克山病重病区富裕县永进村和新发村、尚志市扶安村、五大连池市星火村选择慢型、潜在型克山病患者41名作为病例组,61名健康人作为病区对照组;在非病区望奎县厢兰三村选取48名健康人作为非病区对照组,分别采集清晨空腹外周静脉血检测全血硒水平、全血谷胱甘肽过氧化物酶(GSH-Px)活力、血浆总超氧化物歧化酶(T-SOD)活力及血浆丙二醛(MDA)水平.结果 病例组血硒水平[(34.80±13.30)μg/L]、GSH-Px活力[(104.10±34.19)U/L]、T-SOD活力[(92.16±17.98)×103 U/L]显著低于病区对照组[(41.24±13.57)μg/L、(118.57±25.49)U/L、(104.82±13.56)×103 U/L]和非病区对照组[(48.33±16.51)μg/L、(155.00±24.01)U/L、(108.48±12.73)×103 U/L],组间比较差异有统计学意义(P均<0.05);病例组MDA水平[(7.12±1.37)μmol/L]高于病区对照组[(5.36±1.18)μmol/L]和非病区对照组[(5.22±0.83)μmol/L],组间比较差异有统计学意义(P均<0.05);病区对照组血硒水平、GSH-Px活力低于非病区对照组(P<0.05),组间比较差异均有统计学意义(P<0.05).结论 克山病病人体内存在硒缺乏所致的抗氧化能力降低,氧化损伤增强.氧化应激障碍可能与克山病的心肌损伤有一定的关系.     

地方病调查中随机数字表的应用

在地方病抽样调查过程中,为了获取有代表性的样本,常采用随机化的方法.在实际工作中若调查总体不大的情况下,常通过随机数字表来实现.作者以吉林省克山病病情调查为例介绍随机抽样中随机数字表的使用方法,为地方病现场调查提供参考.     

克山病病区玉米及酱块对大鼠心肌损伤的实验研究

Objective To investigate the myocardial damage in rats fed with corn from Keshan disease area added with bean puree. Methods Male Wistar rats were randomly divided into 3 groups according to their body weights, and fed with corn, corn from Keshan disease area added with bean puree, corn from non-endemic area. The GSH-Px activity of vena cardalis blood was examined in 1 and 3 months, rats were sacrificed after being fed for 6 months to examine the heart changes with HE stain. Results The three groups of GSH-Px activity were different in 1 and 3 months respectively(F=23.60,72.46, all P<0.01); GSH-Px activity was (181.58±22.15), (44.76±28.59)U/L in rats fed with corn, was (195.03±17.66), (30.38±3.35)U/L in those fed with corn added with bean puree from Keshan disease area, lower than the group fed with corn of non-endemic area[(340.90±95.42), (125.17±13.64)U/L, all P < 0.01]. But the difference of GSH-Px activity between simple corn group and corn adding bean puree groups of Keshan disease area was not obvious(P>0.05). Myocardial damage incidence of the three groups was 3/9,1/9,2/7. Difference among three groups did not have statistical significance(χ2=1.33, P> 0.05). Conclusions Only corn from Keshan disease area may induce myocardial damage pathology change. Adding bean puree into corn does not increase damage.