彩色多普勒超声检测胎儿脐血流与胎盘功能定性研究

目的　本课题旨在探讨妊娠晚期胎儿脐血流等参数与胎盘生理或病理性改变的线性关系,用于评估胎盘功能和围产儿预后。方法　通过对60例妊娠晚期（孕周为39±2weeks）的孕妇,经彩色多普勒超声（以下简称彩超）检测胎儿脐血流和腹主动脉血流参数,分别计算并拍片记录其血流阻力指数RI、搏动指数PI、血流收缩期与舒张期比值S/D,并进行统计学分析。随机抽取正常妊娠组,分娩后新鲜胎盘共计20例;异常妊娠组分娩后新鲜胎盘共计7例,统一标准做病理检验。对60例产后新生儿评分追踪记录。结果　胎儿脐血流RI=0.56±0.08,S/D=2.1±0.4,胎盘（功能）成熟老化程度为II°,病检胎盘绒毛模板变性较少,绒毛水肿、结节、钙化为少量;胎儿腹主动脉血流PI=1.48±0.49,S/D=3.1±0.8时,提示胎儿宫内状况良好,新生儿产后评分多为9～10cent/min。结论　胎儿脐血流与胎盘功能存在一定线性关系,其血流参数可作为评价胎盘功能与围产儿预后的一项指标,对指导围产医学具有一定临床价值。     

经皮切吸和胶原酶溶解联合治疗腰椎间盘突出症210例

经皮穿刺腰椎间盘切除术 (ＰＬＤ)和胶原酶髓核溶解术 (ＣＮＬ)是近年来开展的治疗腰椎间盘突出症的简便而效果显著的介入治疗技术[1,2 ] 。我院自1998年 4月～ 2 0 0 0年 2月采用经皮穿刺腰椎间盘切除术和胶原酶髓核溶解术联合治疗腰椎间盘突出症患者 2 10例 ,疗效显著。材料和方法一、一般资料210例患者 ,男性 142例 ,女性 6 8例 ,年龄为 18～ 84岁 ,平均年龄 4 3岁 ,平均病程 1个月～ 2 0年。全部经ＣＴ或ＭＲＩ证实为腰椎间盘突出症 ,并拍摄腰椎组织正侧位Ｘ线片。临床体征检查符合Ｍｃ ｃｕｌｌｏｃｈ[3 ] 制定的标准。患者常见的临床…     

Analysis of TGFBI gene mutation in a Chinese family with atypical Reis-Bückler corneal dystrophy

Objective To identify the TGFBI gene mutation and the relationship between genotype and phenotype of a Chinese family with atypical Reis-Bückler corneal dystrophy (RBCD). Methods Four patients, two non-carrier relatives of the family were enrolled in the present study. In addition to ophthalmologic examinations, PCR amplification and DNA sequencing of exons 4, 11, 12, and 14 of the TGFBI gene were carried out. Exon 14 was also sequenced in 100 healthy controls. Results A G to A transition at eodon 623 in all affected members was identified. This mutation resulted in a substitution of glyeine (GGC) to aspartic acid (GAC) at the protein level. None of the healthy family members, or any of the 100 control subjects carried this mutation. Conclusion The G623D mutation of the TGFBI gene caused an atypical Reis-Buckler corneal dystrophy in this family. This mutation is reported in Chinese for the first time.     

人晶状体上皮细胞HGF与c-Met的表达对增殖和凋亡的影响

目的：探讨肝细胞生长因子(hepatocyte growth factor,HGF)在人晶状体上皮细胞(lens epithelial cell,LEC)的表达及其对晶状体上皮细胞的促增殖和抑制凋亡的作用。方法：取原代培养人晶状体上皮细胞,应用RT-PCR,Western blot检测HGF,C-Met的mRNA及蛋白的表达,应用MTT法检测HGF对晶状体上皮细胞增殖的影响,Western-blot检测：Bcl-2的蛋白表达,以揭示其对晶状体上皮细胞凋亡的影响。结果：HGF和c-Met在人晶状体上皮细胞有表达,HGF有促进晶状体上皮细胞增殖的作用,并能抑制凋亡产生。结论：HGF参与人晶状体上皮细胞的增殖代谢过程,与晶状体上皮细胞的增殖相关。