氯胺T法测定羟脯氨酸含量的方法改进

胶原蛋白是人体内含量最丰富的蛋白质，约占体内总蛋白的1／3[1]。许多疾病都与胶原代谢有关，例如肝纤维化、肺纤维化、系统性硬化等．羟脑氨酸是胶原特有的氨基酸，其含量可代表胶原的多少．但传统的测定方法测定范围窄[2]，结果不稳定．对此，我们进行了部分改进．1材料与方法1．1主要试剂与仪丑1．1．1硼酸—氧化钾缓冲被：61．84g硼酸、225g氯化钾溶于800ml水中，用KOH溶液调pH至8．7，加水至1000ml。1．1．2丙氨酸溶液：10％丙氨酸溶液，用KOH溶液调pH至8．7。1．1．3硼酸—丙氨酸缓冲液：10ml硼酸—氯化钾缓冲液与50ml丙氨酸溶液…     

还原型谷胱甘肽联合甘草酸二铵对腹部开放伤后海水浸泡犬肝功能损伤的治疗作用

Objective To investigate effect of reduced glutathione and diammonium glycyrrhizinate on the treatment of hepatic damage in experimental dogs following open abdominal injuries coupled with seawater immersion. Methods Twenty-four dogs with open abdominal injuries were randomly divided into 3 groups: the control group (given with simple observation after 1.5 h seawater immersion); the routine treatment group (given routine care and fluid transfusion after 1.5 h seawater immersion) ; the hepatic treatment group (given routine are, fluid transfusion and hepatic treatment as well, after 1.5 h seawater immersion). Each group consisted of 8 dogs. Blood samples were taken at different time points to measure total bilirubin (TB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), prothromin time (PT), endotoxin, tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), and pathological examination was also made at the same time. Results For the control group, contents of TB, ALT, AST and LDH increased significantly and survival time was less than 24 hours. Contents of TB, ALT, AST and LDH of the routine treatment group also increased significantly, but survival time of all the experimental animals was mere than 24 hours. Reduced glutathione and diammonium glycyrrhizinate could reduce the extent of lesion quite significantly 12～24 hours following open abdominal injuries. Conclusions Reduced glutathione and diammonium glycyrrhizinate seemed to have good effects on the treatment and prevention against hepatic damage induced by trauma coupled with seawater immersion. It could be used as a primary means for the treatment of such kind of hepatic damage.     

底物—胶原酶—抗体免疫组化方法的建立

传统的免疫组织化学方法是根据抗体与抗原的特异亲和性,利用已知的抗体检测组织中相应抗原的存在状况。酶与其相应的底物同样也具有相似的特异亲和力。根据这种底物—酶的特异亲和作用,我们     

不同免疫佐剂对丙肝核酸疫苗效果的影响

目的：观察不同佐剂对HCV-DNA疫苗效果的影响.方法：雌性BALB/c小鼠分别用脂质体DDAB/EPC和DC-Chol/DOPE、Montanide ISA 720和氢氧化铝为佐剂的HCV-DNA疫苗免疫3次,ELISPOT法观察脾淋巴细胞受HCV核心、E2、E1/E2、NS3和NS5b蛋白刺激后细胞因子的产生.结果：DDAB/EPC组产生IFN-γ较多,在大部分情况下,显著高于其它组.该组IL-2的产生也显著高于其它4组.该组IL-4的产生,在某些情况下也高于其它组.用NS5b刺激,氢氧化铝组IL-4的产生显著高于其它4组（P＜0.05）.裸DNA和两种脂质体组IFN-γ的产生高于IL-4,而Montanide和氢氧化铝组IL-4的产生高于IFN-γ.结论：在4种佐剂中,DDAB/EPC效果最好,Montanide和氢氧化铝可将DNA疫苗Th1为主的免疫特性转换为以Th2为主.     

危重病并发高钠血症20例临床分析

临床急诊中,高钠血症的发病率并不高,一旦出现,提示其病情极为危重,预后差,死亡率高.我们总结了2005年6月至2007年6月收治的20例并发高钠血症危重患者的临床资料,报告如下.     

舒血宁注射液联合孟鲁司特钠对AECOPD患者肺功能及血清4-HNE、bFGF、VCAM-1、TGF-β1水平变化的影响

目的:探讨舒血宁注射液联合孟鲁司特钠对慢性阻塞性肺疾病急性加重期(AECOPD)患者肺功能及血清4-HNE、bFGF、VCAM-1、TGF-β1水平变化的影响。方法:采用随机数字表法将174例AECOPD患者随机分为A组88例和B组86例。A组在常规治疗的基础上给予孟鲁司特钠治疗,B组在A组治疗的基础上给予舒血宁注射液。观察两组治疗前后第1秒用力呼气量(FEV1)、用力肺活量(FVC)、FEV1占FVC的百分比(FEV1/FVC%)、4-羟基壬烯醛(4-HNE)、碱性成纤维细胞生长因子(b-FGF)、转化因子-β(TGF-β1)、细胞内皮黏附因子-1(VCAM-1)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平,比较两组用药过程中的不良反应发生率。结果:B组治疗后FEV1、FEV1/FVC、SOD、GSH-Px水平高于A组(P0.05);4-HNE、b-FGF、TGF-β1、VCAM-1、MDA水平低于A组(P0.05);两组不良反应发生率相比较差异无统计学意义(P0.05)。结论:舒血宁注射液联合孟鲁司特钠治疗AECOPD可改善患者的氧化应激状态,降低TGF-β1、VCAM-1和b-FGF水平,改善肺功能,安全性良好。     

还原型谷胱甘肽联合甘草酸二铵对腹部开放伤后海水浸泡犬肝功能损伤的治疗作用

Objective To investigate effect of reduced glutathione and diammonium glycyrrhizinate on the treatment of hepatic damage in experimental dogs following open abdominal injuries coupled with seawater immersion. Methods Twenty-four dogs with open abdominal injuries were randomly divided into 3 groups: the control group (given with simple observation after 1.5 h seawater immersion); the routine treatment group (given routine care and fluid transfusion after 1.5 h seawater immersion) ; the hepatic treatment group (given routine are, fluid transfusion and hepatic treatment as well, after 1.5 h seawater immersion). Each group consisted of 8 dogs. Blood samples were taken at different time points to measure total bilirubin (TB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), prothromin time (PT), endotoxin, tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), and pathological examination was also made at the same time. Results For the control group, contents of TB, ALT, AST and LDH increased significantly and survival time was less than 24 hours. Contents of TB, ALT, AST and LDH of the routine treatment group also increased significantly, but survival time of all the experimental animals was mere than 24 hours. Reduced glutathione and diammonium glycyrrhizinate could reduce the extent of lesion quite significantly 12～24 hours following open abdominal injuries. Conclusions Reduced glutathione and diammonium glycyrrhizinate seemed to have good effects on the treatment and prevention against hepatic damage induced by trauma coupled with seawater immersion. It could be used as a primary means for the treatment of such kind of hepatic damage.     

腹部开放伤后海水浸泡致犬小肠缺血与缺血再灌注损伤的初步研究

目的 探讨腹部开放伤后海水浸泡致犬肠功能损伤的机制.方法 20只实验犬致伤后随机分为对照组(n=10)和海水浸泡组(n=10).对照组致伤后直接观察,海水浸泡组致伤后立即放人人工配制的海水中.于致伤前(0 h)及致伤后4、8、12、24 h测定实验犬的动脉血压和胃肠黏膜pH值.24 h后取小肠组织测定丙二醛(MDA)、一氧化氮/一氧化氮合酶(NO/NOS)、超氧化物歧化酶(SOD)含量.结果 海水浸泡组动脉血压明显下降,4 h后与对照组比较差异有统计学意义(P<0.05).海水浸泡组肠黏膜pH值在4 h后开始下降,与对照组比较差异有统计学意义(P<0.05);12 h升高,与对照组比较差异无统计学意义(P>0.05).海水浸泡组肠组织MDA、NO/NOS含量海水浸泡组与对照组比较均显著升高(P<0.05),SOD含量则明显降低(P<0.05).结论 肠道缺血和缺血再灌注损伤可能是海水浸泡导致实验犬肠损伤的原因之一.     

肝脏纤维化发生机制的研究

肝肝纤维化的发生机制是近年来肝脏疾病研究的重点之一,目前的研究证明肝脏的星形细胞是肝纤维化时肝内胶原和其它细胞外基质的主要来源。在肝纤维化过程中星形细胞逐渐转化为肌纤维母细胞而产生大量的细胞外基质沉积于肝脏。肌纤维母细胞的收缩可能与门脉高压的形成有一定的关系。星形细胞的转化受TGFβ等许多细胞因子及其它因素的激活。星形细胞合成胶原受多种水平的调节,转录后的调节受α-CP与3'UTR的结合和蛋白因子与5'干环结构结合两方面的影响,合成后的调节受基质金属蛋白酶及其组织抑制物的调节。另外,增生的胶原对星形细胞的反馈抑制功能丧失及肝窦的毛细胞化也对肝纤维化的形成有促进作用。     

肺炎支原体感染患者高迁移率族蛋白B1及其受体表达水平分析

目的探讨肺炎支原体感染患者高迁移率族蛋白B1(High mobility group protein B1,HMGB1)及其受体晚期糖基化终末产物受体(Receptor for advanced glycation end products,RAGE)表达变化及作用机制。方法选择2018年1月-2019年1月南阳市中心医院收治的肺炎支原体感染患者76例作为研究组,另选择同期健康体检者60名作为对照组。采用RT-qPCR实验检测HMGB1及其RAGE表达;采用罗氏E179全自动电化学发光分析仪及配套试剂盒测定降钙素原(Procalcitonin,PCT)含量;采用免疫比浊测定C-反应蛋白(C-reactive protein,CRP)含量。比较两组淋巴细胞绝对值、中性粒细胞绝对值和白细胞计数(white blood cell count,WBC)水平,血浆PCT和CRP水平变化,及HMGB1和RAGE表达变化。结果研究组淋巴细胞绝对值、中性粒细胞绝对值和WBC高于对照组,差异具有统计学意义(P<0.05)。研究组血浆PCT和CRP水平高于对照组,差异具有统计学意义(P<0.05)。研究组HMGB1和RAGE表达水平高于对照组,差异具有统计学意义(P<0.05)。结论肺炎支原体感染患者HMGB1和RAGE表达升高,认为可能参与了疾病的发生、发展过程,可用于早期鉴别肺炎支原体感染,值得临床借鉴。