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排序方式: 共有480条查询结果,搜索用时 15 毫秒
81.
Waldo J. de Boer Hilko van der Voet Bas G.H. Bokkers Martine I. Bakker Polly E. Boon 《Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment》2013,30(11):1433-1449
Various models exist for estimating the usual intake distribution from dietary intake data. In this paper, we compare two of these models, the Iowa State University Foods (ISUF) model and the betabinomial-normal (BBN) model and apply them to three different datasets. Intake data are obtained by aggregating over multiple food products and are often non-normal. The ISUF and BBN model both address non-normality. While the two models have similar structures, they show some differences. The ISUF model includes an additional spline transformation for improving the normality of the intake amount distribution, while the BBN model includes the possibility of addressing covariates, such as age or sex. Our analyses showed that for two of the example datasets both models produced similar estimates of the higher percentiles of the usual intake distribution. However, for the third dataset, where the intake amount distribution appear to be multimodal, both models produced different percentile estimates. 相似文献
82.
Martine Dhilly Javier Becerril‐Ortega Dr. Nathalie Colloc'h Dr. Eric T. MacKenzie Dr. Louisa Barré Prof. Alain Buisson Dr. Olivier Nicole Dr. Cécile Perrio 《Chembiochem : a European journal of chemical biology》2013,14(6):759-769
GluN2B‐containing NMDA receptors are involved in many important physiological functions and play a pivotal role in mediating pain as well as in several neurodegenerative disorders. We aimed to develop fluorescent probes to target the GluN2B subunit selectively in order to allow better understanding of the relationships between receptor localisation and physiological importance. Ifenprodil, known as the GluNR2B antagonist of reference, was chosen as the template for the elaboration of probes. We had previously reported a fluorescein conjugate that was shown (by confocal microscopy imaging of DS‐red‐labelled cortical neurons) to bind specifically to GluN2B. To elaborate this probe, we explored the influence of both the nature and the attachment point of the spacer between the fluorophore and the parent compound, ifenprodil. We performed chemical modifications of ifenprodil at the benzylic position and on the phenol ring by introducing secondary amine or amide functions and evaluated alkyl chains from two to 20 bonds either including or not including secondary amide functions as spacers. The previously developed probe was found to display the greatest activity in the inhibition of NMDA‐induced Ca2+ influx by calcium imaging experiments on HEK293 cells transfected with the cDNA encoding for GluN1‐1A and GluN2B. Further investigations revealed that this probe had a neuroprotective effect equivalent to that of ifenprodil in a standard test for neurotoxicity. Despite effects of lesser amplitude with these probes relative to ifenprodil, we demonstrated that they displaced [3H]ifenprodil in mouse brain slices in a similar manner. 相似文献
83.
84.
Dr. Floriane Gibault Dr. Manon Sturbaut Mathilde Coevoet Dr. Martine Pugnière Dr. Ashley Burtscher Dr. Frédéric Allemand Prof. Patricia Melnyk Prof. Wanjin Hong Prof. Brian P. Rubin Dr. Ajaybabu V. Pobbati Prof. Jean-François Guichou Prof. Philippe Cotelle Dr. Fabrice Bailly 《ChemMedChem》2021,16(18):2823-2844
Starting from our previously reported hit, a series of 1,5-diaryl-1,2,3-triazole-4-carbohydrazones were synthesized and evaluated as inhibitors of the YAP/TAZ-TEAD complex. Their binding to hTEAD2 was confirmed by nanodifferential scanning fluorimetry, and some of the compounds were also found to moderately disrupt the YAP-TEAD interaction, as assessed by a fluorescence polarization assay. A TEAD luciferase gene reporter assay performed in HEK293T cells and RTqPCR measurements in MDA-MB231 cells showed that these compounds inhibit YAP/TAZ-TEAD activity to cells in the micromolar range. In spite of the cytotoxic effects displayed by some of the compounds of this series, they are still good starting points and can be suitably modified into an effective and viable YAP-TEAD disruptor in the future. 相似文献
85.
Enrico Soldati Francesca Rossi Jerome Vicente Daphne Guenoun Martine Pithioux Stefano Iotti Emil Malucelli David Bendahan 《International journal of molecular sciences》2021,22(5)
Bone microarchitecture has been shown to provide useful information regarding the evaluation of skeleton quality with an added value to areal bone mineral density, which can be used for the diagnosis of several bone diseases. Bone mineral density estimated from dual-energy X-ray absorptiometry (DXA) has shown to be a limited tool to identify patients’ risk stratification and therapy delivery. Magnetic resonance imaging (MRI) has been proposed as another technique to assess bone quality and fracture risk by evaluating the bone structure and microarchitecture. To date, MRI is the only completely non-invasive and non-ionizing imaging modality that can assess both cortical and trabecular bone in vivo. In this review article, we reported a survey regarding the clinically relevant information MRI could provide for the assessment of the inner trabecular morphology of different bone segments. The last section will be devoted to the upcoming MRI applications (MR spectroscopy and chemical shift encoding MRI, solid state MRI and quantitative susceptibility mapping), which could provide additional biomarkers for the assessment of bone microarchitecture. 相似文献
86.
Martine Morzel Gerald F Fitzgerald Elke K Arendt 《Food research international (Ottawa, Ont.)》1997,30(10):777-785
The influence of five strains of lactic acid bacteria (four Lactobacillus and one Carnobacterium) on the quality of fermented salmon fillets was studied. Best starter growth (increase of more than 1 log in 3 days) and acidification of muscle (e.g. pH reduction of approximately 0.7 units in 5 days) were achieved with the two commercial strains L. sake LAD and L. alimentarius BJ33. pH reduction was consistently lower (e.g. reduction of 0.2 units in 5 days) with C. piscicola 85. Protein breakdown as observed on SDS-PAGE gels was similar for all strains. In contrast, the starter strain did influence texture and colour changes. Fast acidifying strains L. sake LAD and L. alimentarius BJ33 brought about a firmer overall texture and a lighter colour, while softening of flesh occurred in samples processed with C. piscicola 85. Sensory evaluations indicated that samples processed with fast acidifying strains were preferred. L. sake LAD and L. alimentarius BJ33 are regarded as suitable starters for fermentation of salmon fillets. 相似文献
87.
Cadet Bernard; Chossière Jacqueline; Berthelier Isabelle; écolasse Martine 《Canadian Metallurgical Quarterly》1995,49(1):61
Studied the influence of various cognitive modalities on risk assessment. Human Ss: 78 normal male and female French adults (university students). Ss were presented with information related to a potential environmental danger and asked to evaluate the degree of risk. The information involved 3 categories of data presented under 3 experimental conditions: representativeness, availability, and anchoring of the relevant information. A control condition offering no information on which to base the risk assessment was also included. Subjective evaluations of the degree of risk served as the dependent variable. (English abstract) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
88.
C Graille G Shlyck P Buser I Kozlovskaia A Rougeul-Buser 《Canadian Metallurgical Quarterly》1998,28(1-2):52-60
Electrocorticographic recordings were taken from the posterior parietal cortical area in monkeys performing a variety of tasks on ground and in space flight. The question addressed here was whether ECoG analysis would be a reliable index of attention to the learned task. It is shown that measuring the instantaneous frequency, rather than instrumentally evaluating the spectral content of the ECoG, may provide useful information on the attentional states of the animals. Investigations on Rhesus performed in Paris provided a useful database to evaluate the more complex results obtained in the Russian experiments with space flights, during which, according to the ECoG marker, some attentional disorders occurred. It is not unreasonable to envisage using similar evaluation in the scalp EEG in human flights in the future. 相似文献
89.
Patrice Therond Martine Couturier Jean-François Demelier Frédérique Lemonnier 《Lipids》1993,28(3):245-249
A method for the simultaneous determination of the main molecular species of soybean phosphatidylcholine or phosphatidylethanolamine
and their corresponding hydroperoxides is described. Hydroperoxides were formed by incubation of phospholipids with lipoxygenase
at pH 9.2. Silicic acid column chromatography (silica Sep-Pak column) was used to separate the phospholipids into phosphatidylcholine
and phosphatidylethanolamine. A single C−18 reverse-phase column was employed to separate the main molecular species of soybean
phosphatidylcholine or phosphatidylethanolamine and their hydroperoxides by high-performance liquid chromatography. The mobile
phase consisted of 5% 10 mM ammonium acetate at pH 5 and 95% methanol. The molecular species of phosphatidylcholine and phosphatidylethanolamine
were detected at 205 nm; the eluate was mixed with a chemiluminescence reagent (isoluminol and microperoxidase) and monitored
by fluorometry. Under the experimental conditions used, three individual molecular species of both soybean phosphatidylethanolamine
and phosphatidylcholine (18∶3/18∶2, 18∶2/18∶2 and 16∶0/18∶2), together with their corresponding hydroperoxides, were identified
and quantitated. 相似文献
90.
Pascale Cogrel Isabelle Morel Gerard Lescoat Martine Chevanne Pierre Brissot Pierre Cillard Josiane Cillard 《Lipids》1993,28(2):115-119
The response of normal and transformed rat hepatocytes to oxidative stress was investigated. Isolated normal rat hepatocytes
and differentiated hepatoma cells (the Fao cell line was derived from the Reuber H 35 rat hepatoma) in suspension were incubated
with the ADP/Fe3+ chelate for 30 min at 37°C. Membrane lipid oxidation was assessed by measuring (i) free malondialdehyde (MDA) production
by a high-performance liquid chromatography (HPLC) procedure, (ii) membrane fatty acid disappearance as judged by capillary
gas chromatography, and (iii) α-tocopherol oxidation as determined by HPLC and electrochemical detection. The addition of
iron led to increased MDA production in normal as well as in transformed cells, and to simultaneous consumption of polyunsaturated
fatty acids (PUFA) and α-tocopherol. In addition, in Fao cells more α-tocopherol was consumed during lipid peroxidation while
less PUFA was oxidized. Lipid peroxidation was lower in tumoral hepatocytes than in normal cells. This could be due to a difference
in membrane lipid composition because of a lower PUFA content and a higher α-tocopherol level in Fao cells. During oxidation,
Fao cells produced 1.5 to 2 times less MDA than normal cells, while in the tumoral cells the amount of oxidized PUFA having
3 or more double bonds was 7 to 8 times lower. Therefore, measuring MDA alone as an index of lipid peroxidation did not allow
for proper comparison of the membrane lipid oxidizability of transformed cellsvs. the membrane lipid oxidizability of normal cells. 相似文献