Interpreting spatial and temporal neural activity through a recurrent neural network brain-machine interface.

We propose the use of optimized brain-machine interface (BMI) models for interpreting the spatial and temporal neural activity generated in motor tasks. In this study, a nonlinear dynamical neural network is trained to predict the hand position of primates from neural recordings in a reaching task paradigm. We first develop a method to reveal the role attributed by the model to the sampled motor, premotor, and parietal cortices in generating hand movements. Next, using the trained model weights, we derive a temporal sensitivity measure to asses how the model utilized the sampled cortices and neurons in real-time during BMI testing.     

Developmental change in the acuity of the "number sense": The approximate number system in 3-, 4-, 5-, and 6-year-olds and adults.

Behavioral, neuropsychological, and brain imaging research points to a dedicated system for processing number that is shared across development and across species. This foundational Approximate Number System (ANS) operates over multiple modalities, forming representations of the number of objects, sounds, or events in a scene. This system is imprecise and hence differs from exact counting. Evidence suggests that the resolution of the ANS, as specified by a Weber fraction, increases with age such that adults can discriminate numerosities that infants cannot. However, the Weber fraction has yet to be determined for participants of any age between 9 months and adulthood, leaving its developmental trajectory unclear. Here we identify the Weber fraction of the ANS in 3-, 4-, 5-, and 6-year-old children and in adults. We show that the resolution of this system continues to increase throughout childhood, with adultlike levels of acuity attained surprisingly late in development. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Contextual control over conditioned responding in an extinction paradigm.

Four experiments studied contextual control over rats' freezing to conditioned stimuli (CSs) that had been paired with shock and were then extinguished. In Experiment 1, rats were exposed to a CS A–shock and a CS B–shock pairing in Context C. CS A was then extinguished in Context A, and CS B in Context B. Freezing was renewed when each CS was presented in the context where the other CS had been extinguished. In Experiments 2–4, rats were exposed to a CS A–shock pairing in A and a CS B–shock pairing in B. They were then exposed to Context C where one, both, or neither of the CSs were extinguished, or where both CSs continued to be reinforced. On test, the rats froze more to CS A than to CS B in Context A, and more to CS B than to CS A in Context B, but only if the CSs had been extinguished. Thus, after extinction, rats use contexts to regulate retrieval not only of their memory for extinction, but also of their memory for the original conditioning episode. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Horizontal bubbly flow with elbow restrictions: Interfacial area transport modeling

The present study develops an interfacial area transport equation applicable to an air-water horizontal bubbly flow, along which two types of horizontal elbows are installed as flow restrictions. Two sets of experiments are performed in a round glass tube of 50.3 mm inner diameter. Along the test section, a 90-degree elbow is installed at L/D = 206.6 from the two-phase mixture inlet and then a 45-degree elbow is installed at L/D = 353.5. In total, 15 different flow conditions in the bubbly flow regime for each of the two flow restriction experiments are studied. Detailed local two-phase flow parameters are acquired by a double-sensor conductivity probe at four different axial locations in the 90-degree experiment and three different axial locations in the 45-degree experiment. The effect of the elbows is found to be evident in the distribution of local parameters as well as in the development of interfacial structures. It is clear that the elbows make an effect on the bubble interactions resulting in significant changes to both the void fraction and interfacial area concentration. In the present analysis, the interfacial area transport equation is developed in one-dimensional form via area averaging. In the averaging process, characteristic non-uniform distributions of the flow parameters in horizontal two-phase flow are treated mathematically through a distribution parameter. The mechanistic models for the major bubble interaction phenomena developed in vertical flow analysis are employed in the present study. Furthermore, the change in pressure due to the minor loss of an elbow is taken into consideration by using a newly developed correlation analogous to Lockhart and Martinelli's. In total, 105 area-averaged data points are employed to benchmark the present model. The present model predicts the data relatively well with an average percent difference of approximately ±20%.     

SAR型模数转换器在电机控制电流测量中的应用

在电机控制系统中,设计者可通过周密分析获得理想的性能,即使开始看上去某些元件不合适.对于来自电流传感器的±5 V输出信号,则需要使用双电源(±12 V)的模数(A/D)转换器,TI公司推出的ADS7864型A/D转换器拥有同样效能,并给出了相关闭环电流互感器的应用.     

互联网下印刷业的新机遇

互联网的存在，不仅能提升资讯，取得与双方间资讯交换的速度，更解决了资讯共享的瓶颈，因此所有的传统工业无不争先恐后的在互联网中探寻，希望能从中找到企业新一轮的竞争力。     

Mechanistic Analysis of an Engineered Enzyme that Catalyzes the Formose Reaction

An enzyme that catalyzes the formose reaction, termed “formolase”, was recently engineered through a combination of computational protein design and directed evolution. We have investigated the kinetic role of the computationally designed residues and further characterized the enzyme's product profile. Kinetic studies illustrated that the computationally designed mutations were synergistic in their contributions towards enhancing activity. Mass spectrometry revealed that the engineered enzyme produces two products of the formose reaction—dihydroxyacetone and glycolaldehyde—with the product profile dependent on the formaldehyde concentration. We further explored the effects of this product profile on the thermodynamics and yield of the overall carbon assimilation from the formolase pathway to help guide future efforts to engineer this pathway.     

Synthesis and electrical properties of the (PVA)0.7(KI)0.3·xH2SO4 (0 ≤ x ≤ 5) polymer electrolytes and their performance in a primary Zn/MnO2 battery

Solid acid polymer electrolytes (SAPE) were synthesised using polyvinyl alcohol, potassium iodide and sulphuric acid in different molar ratios by solution cast technique. The temperature dependent nature of electrical conductivity and the impedance of the polymer electrolytes were determined along with the associated activation energy. The electrical conductivity at room temperature was found to be strongly depended on the amorphous nature of the polymers and H₂SO₄ concentration. The ac (100 Hz to 10 MHz) and dc conductivities of the polymer electrolytes with different H₂SO₄ concentrations were analyzed. A maximum dc conductivity of 1.05 × 10⁻³ S cm⁻¹ has been achieved at ambient temperature for electrolytes containing 5 M H₂SO₄. The frequency and temperature dependent dielectric and electrical modulus properties of the SAPE were studied. The charge transport in the present polymer electrolyte was obtained using Wagner's polarization technique, which demonstrated the charge transport to be mainly due to ions. Using these solid acid polymer electrolytes novel Zn/SAPE/MnO₂ solid state batteries were fabricated and their discharge capacity was calculated. An open circuit voltage of 1.758 V was obtained for 5 M H₂SO₄ based Zn/SAPE/MnO₂ battery.     

新西兰斜坡屋顶花园

<正>新西兰馆的主题是"自然之城:生活在天与地之间"。故事从一个凉棚说起。凉棚始于一条欢迎走廊。建筑设计师布莱尔(Blair)想让"参观者体验新西兰城市和大自然,进入展馆之后,可以沿着一条弯弯曲曲的斜坡,渐渐往上走。新西兰的风景、森林、海洋、山脉、城市和人……"最后,人们将会走上屋顶。正是在这里,布莱尔为景观设计师提供了一个大舞台。     

Interaction of the σ2 Receptor Ligand PB28 with the Human Nucleosome: Computational and Experimental Probes of Interaction with the H2A/H2B Dimer

Sigma‐2 (σ₂) binding sites are an emerging target for anti‐neoplastic agents due to the strong apoptotic effect exhibited by σ₂ agonists in vitro and the overexpression of these sites in tumor cells. Nonetheless, no σ₂ receptor protein has been identified. Affinity chromatography using the high‐affinity σ₂ ligand PB28 and human SK‐N‐SH neuroblastoma cells was previously utilized to identify σ₂ ligand binding proteins, specifically histones H1, H2A, H2B, and H3.3a. To rationalize this finding, homology modeling and automated docking studies were employed to probe intermolecular interactions between PB28 and human nucleosomal proteins. These studies predicted interaction of PB28 with the H2A/H2B dimer at a series of sites previously found to be implicated in chromatin compaction and nucleosomal assembly. To experimentally verify this prediction, a competitive binding assay was performed on the reconstituted H2A/H2B dimer using [³H]PB28 as radioligand, and an IC₅₀ value of 0.50 nM was determined for PB28 binding. In addition, [³H]PB28 was found to accumulate with up to a fivefold excess in nuclear fractions over cytosolic fractions of SK‐N‐SH and MCF7 cells, indicating that PB28 is capable of entering the nucleus to interact with histone proteins. In conjunction with computational results, these data suggest that PB28 may exert its cytotoxic effect through direct interaction with nuclear material.