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91.
Bose D Durgbanshi A Martinavarro-Domínguez A Capella-Peiró ME Carda-Broch S Esteve-Romero J Gil-Agustí M 《Journal of pharmacological and toxicological methods》2005,52(3):323-329
INTRODUCTION: Amitriptyline and nortriptyline are tricyclic antidepressants which act by enhancing the actions of norepinephrine and serotonin caused by blocking the re-uptake of various neurotransmitters at the neuronal membrane. A micellar liquid chromatographic procedure was developed to determine these drugs in serum samples for use in clinical monitoring. METHODS: The chromatographic determination of these highly hydrophobic substances was carried out using a 0.15 M SDS-6% (v/v) pentanol buffered at pH 7, in a C18 column, and electrochemical detection at 650 mV. The flow-rate was 1.5 mL/min. The analysis time was 14 min. RESULTS: The limits of detection (ng/mL) in serum were 0.25 and 0.31 for amitriptyline and nortriptyline, respectively. Repeatability and intermediate precision were evaluated at three different concentrations in serum samples. DISCUSSION: Untreated serum samples were injected directly into the HPLC system after filtration, leading to be a simple procedure that can be applied in routine analyses for Therapeutic Drug Monitoring. 相似文献
92.
Gaurav Ameta Amit Upadhyay Sunil Gothwal Kuldeep Singh Kirti Dubey Abhilasha Gupta 《Indian journal of pediatrics》2013,80(3):195-198
Objective
To compare reduction in incidence of feed intolerance in neonates born with meconium stained amniotic fluid (MSAF) by use of gastric lavage to those who did not receive lavage.Methods
This Randomized controlled trial was conducted in all vigorous newborns delivered through MSAF, with birth weight ≥1800 g and gestation ≥34 wk. In the lavage group, gastric lavage with 10 ml/kg of normal saline was done.Results
Twelve neonates in the lavage group (n?=?124) developed feed intolerance compared to 16 neonates in control group (n?=?120), (p?=?.309; OR 0.69; 95%CI 0.27–1.58). No difference in any other morbidity was noted.Conclusions
Gastric lavage in neonates with MSAF does not reduce feed intolerance, irrespective of thickness of MSAF and it confers no advantages. 相似文献93.
Neuropathic pain triggers a cascade of events in the sensory neurons. It is the main complication of diabetes after cardiovascular disease. Nitric oxide (NO) produced from nitric oxide synthases (NOS) is an important signaling molecule which is crucial for many physiological processes such as synaptic plasticity, neuronal survival, vasodilation, vascular homeostasis, immune regulation. Overproduction of NO due to changes in NOS isoforms level involves pathological processes such as neurotoxicity, septic shock and neuropathic pain. All three isoforms of NOS as well as their end product, NO have modulatory effect on neuropathic pain. Overactivation of the N-Methyl-d-Aspartate receptor and peroxynitrite formation results in high levels of neuronal NOS (nNOS) and endothelial NOS (eNOS) which suggest that nNOS and eNOS are critical for pain hypersensitivity. Inducible NOS induced in glia by inflammation due to activation of Tumor Necrosis Factor α, Calcitonin Gene Regulating Peptide, Mitogen Activated Protein Kinases, Extracellular signal Regulated Kinase, c-Jun N-terminal kinases can induce neuronal death. This review focuses on different nitric oxide synthases and their role in pathophysiology of neuropathic pain considering NOS as an important therapeutic target. 相似文献
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95.
Tran Vu Thieu Nga Abhilasha Karkey Sabina Dongol Hang Nguyen Thuy Sarah Dunstan Kathryn Holt Le Thi Phuong Tu James I Campbell Tran Thuy Chau Nguyen Van Vinh Chau Amit Arjyal Samir Koirala Buddha Basnyat Christiane Dolecek Jeremy Farrar Stephen Baker 《BMC infectious diseases》2010,10(1):125
Background
PCR amplification for the detection of pathogens in biological material is generally considered a rapid and informative diagnostic technique. Invasive Salmonella serovars, which cause enteric fever, can be commonly cultured from the blood of infected patients. Yet, the isolation of invasive Salmonella serovars from blood is protracted and potentially insensitive.Methods
We developed and optimised a novel multiplex three colour real-time PCR assay to detect specific target sequences in the genomes of Salmonella serovars Typhi and Paratyphi A. We performed the assay on DNA extracted from blood and bone marrow samples from culture positive and negative enteric fever patients.Results
The assay was validated and demonstrated a high level of specificity and reproducibility under experimental conditions. All bone marrow samples tested positive for Salmonella, however, the sensitivity on blood samples was limited. The assay demonstrated an overall specificity of 100% (75/75) and sensitivity of 53.9% (69/128) on all biological samples. We then tested the PCR detection limit by performing bacterial counts after inoculation into blood culture bottles.Conclusions
Our findings corroborate previous clinical findings, whereby the bacterial load of S. Typhi in peripheral blood is low, often below detection by culture and, consequently, below detection by PCR. Whilst the assay may be utilised for environmental sampling or on differing biological samples, our data suggest that PCR performed directly on blood samples may be an unsuitable methodology and a potentially unachievable target for the routine diagnosis of enteric fever.96.
Sood A Chadha VD Dhawan DK 《Journal of environmental pathology, toxicology and oncology》2011,30(2):153-162
The present study elucidated the protective potential of selenium following 131I-induced alterations in rat blood. Forty rats were segregated into 4 groups. Animals in Group I served as normal controls, Group II animals were injected with a single dose of 3.7 Mbq of 131I (carrier free), Group III animals were supplemented with selenium (1 ppm), and Group IV animals were given a combined treatment of selenium and 131I. 131I treatment of rats showed significant increases in total leukocyte counts (TLCs), lymphocytes, and neutrophils (monocytes and eosinophils were not recorded). These were significantly restored upon supplementation of selenium. Lipid peroxidase (LPO), glutathione peroxidase (GSH-PX), reduced glutathione (GSH), superoxide dismutase (SOD), and δ-aminolevulinic acid dehydratase (δ-ALAD) were found to be enhanced following 131I treatment. However, the levels of catalase were found to be decreased. Selenium administration to 131I-treated rats resulted in significant restoration of these enzyme activities. Scanning electron microscope (SEM) studies also revealed various surface deformities in erythrocytes after 131I treatment, which upon supplementation with selenium were significantly restored. In conclusion, selenium may prove to be an effective radioprotector following 131I treatment. 相似文献
97.
Gupta Abhilasha Verma Aruna Kashyap Monika Gautam Priti 《Journal of obstetrics and gynaecology of India》2020,70(1):18-22
The Journal of Obstetrics and Gynecology of India - To evaluate the efficacy and safety of ART in prevention of mother-to-child transmission of HIV. All pregnant females who were tested and found... 相似文献