全文获取类型
收费全文 | 28930篇 |
免费 | 1825篇 |
国内免费 | 426篇 |
学科分类
医药卫生 | 31181篇 |
出版年
2024年 | 11篇 |
2023年 | 162篇 |
2022年 | 239篇 |
2021年 | 1272篇 |
2020年 | 595篇 |
2019年 | 872篇 |
2018年 | 1021篇 |
2017年 | 810篇 |
2016年 | 1193篇 |
2015年 | 1595篇 |
2014年 | 1864篇 |
2013年 | 2101篇 |
2012年 | 2942篇 |
2011年 | 2811篇 |
2010年 | 1776篇 |
2009年 | 1443篇 |
2008年 | 1852篇 |
2007年 | 1773篇 |
2006年 | 1493篇 |
2005年 | 1324篇 |
2004年 | 1064篇 |
2003年 | 846篇 |
2002年 | 720篇 |
2001年 | 308篇 |
2000年 | 241篇 |
1999年 | 172篇 |
1998年 | 107篇 |
1997年 | 70篇 |
1996年 | 75篇 |
1995年 | 38篇 |
1994年 | 39篇 |
1993年 | 35篇 |
1992年 | 51篇 |
1991年 | 35篇 |
1990年 | 30篇 |
1989年 | 29篇 |
1988年 | 20篇 |
1987年 | 18篇 |
1986年 | 21篇 |
1985年 | 19篇 |
1984年 | 13篇 |
1983年 | 7篇 |
1982年 | 6篇 |
1981年 | 7篇 |
1978年 | 8篇 |
1973年 | 5篇 |
1972年 | 4篇 |
1971年 | 6篇 |
1970年 | 4篇 |
1968年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 23 毫秒
71.
Yu Ri An Jeong Han Seung Jun Kim Moon-Ju Oh Jung-Hwa Oh Seok-Joo Yoon Seung Yong Hwang 《Toxicology and Environmental Health Sciences》2010,2(4):245-250
It is important to acknowledge the harmful effects of environmental chemicals in human’s lives. The toxic effects of Diethylstilbestrol (DES), one of the endocrine-disrupting chemicals (EDCs), have been documented in many studies. As expected, DES affect male gendal hormone as well as female’s; therefore, epigenetic study should be considered. In this study, microarray technology was used to study harmful effects on the level of genomics, and here, two types of microarray chips- the Agilent mouse genome 4 × 44 K array for gene expression profiling and the Agilent mouse miRNA v13 for miRNA expression profiling-was used to study the relation between gene and miRNA expression profiles. As a result, we identified 4 miRNAs (miR 203, 350, 421, and 466i) that were similarly expressed at 3 hrs and 24 hrs of DES treat times. Twenty one genes matched between predicted target for 4 miRNAs and 118 genes expressed similarly. These genes have functions related to cell differentiation and cell cycle. Therefore, DES affects cellular function and induces toxicity in TM4 cells. In future studies, it is necessary to find more related functions and mechanisms of DES in the system. 相似文献
72.
73.
123I-MIBG myocardial scintigraphy as a noninvasive screen for the diagnosis of coronary artery spasm
Jong-Won Ha Jong-Doo Lee Yangsoo Jang Namsik Chung June Kwan Se-Joong Rim Young-Joon Lee Won-Heum Shim Seung-Yun Cho Sung-Soon Kim 《Journal of nuclear cardiology》1998,5(6):591-597
Background It has been suggested that the sympathetic nervous system might play an important role in the development of coronary artery
spasm. However, no cardiac imaging modality has been able to demonstrate abnormal sympathetic innervation in patients with
coronary artery spasm. The purpose of this study was to assess the presence and location of abnormal sympathetic innervation
using iodine 123-metaiodobenzylguanidine (123I-MIBG) single photon emission computed tomography (SPECT) and to evaluate the clinical efficacy of 123I-MIBG SPECT as a noninvasive screening test in patients with coronary artery spasm.
Methods and Results Coronary arteriography and a provocative test with intravenous administration of ergonovine maleate were performed in 26 patients
(20 men, 6 women, mean age 48.2±12.0 years, range 20 to 67 years) who were suspected of having a coronary artery spasm. The
subjects were divided into 2 groups: group 1 (n=18) comprised subjects with negative provocative provocative test result,
and group 2 (n=8) comprised subjects with negative provocative test results. Ten healthy subjects served as controls. No abnormal
MIBG uptake was observed in the control subjects. Abnormal sympathetic nervous innervation using 123I-MIBG SPECT was observed either as a reduced uptake or a defective pattern in the perfused areas in 13 of the 18 regions
supplied by vessels of ergonovine-induced vasospasm. Normal sympathetic innervation, as evidenced by normal 123I-MIBG uptake, was noted in all of the 60 segments of normal vessel territories. Reduced uptake of 123I-MIBG was not detected in the perfused areas of 5 vasospasm-induced vessels (perfusion territory of left anterior descending
coronary artery [LAD] and the right coronary artery [RCA] in 2 and 3 patients, respectively). The sensitivity and specificity
of 123I-MIBG for detection of coronary artery spasm were 72.2% (95% confidence interval, [CI] 55% to 89%) and 100%, respectively.
The positive predictive and negative predictive values were 100% and 92.3% (95% CI 91% to 93%), respectively.
Conclusion
123I-MIBG SPECT is a feasible method to evaluate noninvasively and localize the territories of coronary arteries with spasm.
Invasive diagnostic coronary arteriography with ergonovine provocation test may be unnecessary for diagnosis of coronary artery
spasm in patients with typical resting pain, negative exercise test or normal thallium perfusion scan results, but showing
abnormalities in 123I-MIBG SPECT.
Presented in part at the European Association of Nuclear Medicine Congress, September 1996, Copenhagen, Denmark. 相似文献
74.
75.
Jae Sue Choi Han Suk Young Jong Cheol Park Jin-Ho Choi Won Sick Woo 《Archives of pharmacal research》1986,9(4):233-236
The flavonoids isolated from the leaves ofRhododendron brachycarpum, were identified as quercetin, avicularin, quercitrin and hyperin. 相似文献
76.
77.
Malaria was steadily decreasing in Korea except in certain counties of the mountainous and hilly areas, in the 1960s. Judging from the present epidemiological, social and economic conditions, it can be said with confidence that malaria with "unstable" characteristic in the Republic of Korea has already been disappeared. No doubt, the causes of the disappearance of malaria are complex. Certainly improved living conditions and life style; better medical and educational facilities in the wake of a rapid economic development could all have some role. On the other hand, the disappearance of malaria without large scale control operations could be ascribed to the two main factors: one is malaria case detection and simultaneous drug therapy available through the nation-wide passive case detection network during the 1960s and the other is rapidly improved farming practices begun in 1970s, which resulted in the use of a huge quantity of pesticides and other chemicals for agriculture, which, in turn, might affect local anopheline vectors which were originally not effective ones any way. 相似文献
78.
Overexpression of S100A4 is closely related to the aggressiveness of gastric cancer 总被引:25,自引:0,他引:25
Cho YG Nam SW Kim TY Kim YS Kim CJ Park JY Lee JH Kim HS Lee JW Park CH Song YH Lee SH Yoo NJ Lee JY Park WS 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2003,111(5):539-545
Elevated levels of the calcium-binding protein S100A4 cause metastasis of benign rat mammary tumor cells. To investigate whether S100A4 plays an important role in the invasion and metastasis of gastric cancers, we examined the gene mutations in the coding regions and expression patterns of the S100A4 in gastric adenocarcinoma in Korea. Moderate to strong expression of S100A4 was found in 53 (68.8%) of the 77 gastric adenocarcinomas, whilst normal gastric epithelium either failed to stain or showed weak staining. Interestingly, S100A4 expression was more frequently observed in gastric cancer patients with advanced gastric cancer (p=0.039), positive lymph node metastasis (p=0.001), and peritoneal dissemination (p=0.022). No gene mutations were found in the analyzed genomic area in 77 gastric adenocarcinomas and 15 gastric cancer cell lines. We found one single nucleotide polymorphism without an amino acid change, A99G, in two cases. These data suggest that the overexpression of S100A4 may be closely related to the aggressiveness of gastric cancer in Korea. 相似文献
79.
Perivascular epithelioid cell tumor of the uterus: immunohistochemical, ultrastructural and molecular study 总被引:1,自引:0,他引:1
A case of perivascular epithelioid cell tumor of the uterus is reported, occurring in a 32-year-old woman. The tumor (8.0 cm in dimension) showed exophytic growth from the outer half of the myometrium. Histopathologically, the tumor was composed of thick blood vessels and perivascular epithelioid cells. The neoplastic cells were strongly immunoreactive for HMB45 antigen, CD117 (c-kit), vimentin and the progesterone receptor, but completely negative for S-100 protein, smooth muscle actin, desmin, CD34, the estrogen receptor and p16. The Ki-67 labeling index was low (1.25%). Ultrastructurally, the neoplastic cells had numerous premelanosomes with some glycogen deposits. Single-stranded DNA conformational polymorphism of p53 and methylation-specific polymerase chain reaction of p16 revealed negative results. Definite melanosomes on electron microscopic analysis and coexpression of HMB45 antigen and stem cell factor receptor (CD117) may provide the clue to understanding perivascular epithelioid cell tumor because angiomyolipoma also coexpresses HMB45 antigen and CD117. 相似文献
80.
The application of molecular techniques to solid tumors 总被引:1,自引:0,他引:1
Recent developments in the field of molecular biology including the sequencing of the human genome and related high throughput methodologies are presenting the diagnostic pathologist with new opportunities to expand our understanding of human disease. These techniques enable the comprehensive assessment of molecular alterations with cell populations of interest, including cancer. It will be necessary for the diagnostic pathologist to become familiar with these techniques to effectively translate their potential into the clinical environment. 相似文献