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Carreira Micael Ting Karine Lan Hing Csobanka Petra Gonçalves Daniel 《Universal Access in the Information Society》2017,16(3):561-580
Universal Access in the Information Society - In-air gestural interfaces are gaining popularity due to the increasing availability and low cost of gesture recognition hardware. However, the current... 相似文献
33.
Katharina N?ske Hans-Jürgen Stark Leonard Nevaril Manuel Berning Lutz Langbein Ashish Goyal Sven Diederichs Petra Boukamp 《International journal of molecular sciences》2016,17(2)
Despite decades of skin research, regulation of proliferation and homeostasis in human epidermis is still insufficiently understood. To address the role of mitoses in tissue regulation, we utilized human long-term skin equivalents and systematically assessed mitoses during early epidermal development and long-term epidermal regeneration. We now demonstrate four different orientations: (1) horizontal, i.e., parallel to the basement membrane (BM) and suggestive of symmetric divisions; (2) oblique with an angle of 45°–70°; or (3) perpendicular, suggestive of asymmetric division. In addition, we demonstrate a fourth substantial fraction of suprabasal mitoses, many of which are committed to differentiation (Keratin K10-positive). As verified also for normal human skin, this spatial mitotic organization is part of the regulatory program of human epidermal tissue homeostasis. As a potential marker for asymmetric division, we investigated for Numb and found that it was evenly spread in almost all undifferentiated keratinocytes, but indeed asymmetrically distributed in some mitoses and particularly frequent under differentiation-repressing low-calcium conditions. Numb deletion (stable knockdown by CRISPR/Cas9), however, did not affect proliferation, neither in a three-day follow up study by life cell imaging nor during a 14-day culture period, suggesting that Numb is not essential for the general control of keratinocyte division. 相似文献
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Capillary zone electrophoresis (CZE) has been applied to separation and characterisation of enzymatic (tryptic) hydrolysates of water-soluble proteins from Bacillus thuringiensis (Bt)-transgenic (Aristis-Bt) and two native non-transgenic (Aristis and Coventry) maize varieties. Water-soluble proteins were extracted from the flour of these maize species and digested by bovine pancreatic trypsin immobilised on agarose gel in 100 mM ammonium hydrocarbonate buffer, pH 7.9. The yielded tryptic digests of proteins were analysed by CZE in four acidic background electrolytes (BGEs) (100 mM H3PO4, 50 mM Tris, pH 2.25; 500 mM acetic acid, pH 2.54; 200 mM formic acid, 200 mM acetic acid, pH 2.05; and 200 mM iminodiacetic acid, pH 2.26) using a lab-made CZE apparatus equipped with bare fused silica capillary and UV-absorption detector operating at 206 nm. Among the tested BGEs, the best resolution of the tryptic peptides of extracted proteins of the above three maize species was obtained in isoelectric BGE, 200 mM iminodiacetic acid, pH 2.26. Selected resolved tryptic peptides of proteins were characterised by effective electrophoretic mobilities and corrected (migration times normalised) peak areas. Some significant relative qualitative and quantitative differences in CZE-UV profiling of tryptic protein digests were found, which can be potentially used to differentiate transgenic Aristis Bt and non-transgenic Aristis varieties or two native non-transgenic varieties, Aristis and Coventry. 相似文献
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Sally Zhao 《缤纷家居》2014,(7):130-135
Extract荷兰Den Bosh的一怕老建筑,最近迎来自己的新生。那是座建于20世纪的双层Loft,在设计师Roderick Vos的改造下,它的空间一半被用于零售展示,一半用于办公思考。它模糊了设计师与顾客、艺术与生活的界限,令每一位到访者都能在幽默而单纯的氛围中,领略双享空间的专业设计精神。 相似文献
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Suburbanization is one of the most important processes changing the nature of metropolitan regions in Europe’s post-socialist countries. This paper evaluates the importance of social contacts of new suburbanites on local level social cohesion and development. The paper employs examples from empirical research into new suburban communities in the Prague metropolitan region of the Czech Republic. Our results show that both internal and external social ties are developing in such newly built areas, which is important for the well-being of both the neighborhood and the municipality. Internal ties foster the social cohesion of a neighborhood, while external ties encourage development of the whole municipality (e.g., via political participation or informal contacts). 相似文献
37.
Petra Lindemann-Matthies 《Landscape Research》2017,42(5):482-497
This study investigates with the help of a manipulative experiment how people with different nationalities perceive meadow-like arrays of different species richness (1, 8, 16, 32, 64 species). Participants (n = 187) were all residents of the canton of Zurich and originated from 43 different countries. Overall valuation of the meadow-like arrays increased with species richness. However, while participants from high-income countries (measured by GDP per capita) clearly liked species-rich meadows most and species-poor ones least, participants from low-income countries did not have such clear preferences. Preferences were not related to the length of time a participant had already spent in Switzerland (on average seven years), but to perceived familiarity with a meadow-like array. Sex and education did not influence valuations. Environmental expertise fostered participants’ dislike of species-poor arrays, as did age. 相似文献
38.
Petra Fallier-Becker Maike Nieser Ulrike Wenzel Rainer Ritz Susan Noell 《International journal of molecular sciences》2016,17(8)
The astrocytic endfoot membranes of the healthy blood-brain barrier—contacting the capillary—are covered with a large number of the water channel aquaporin 4 (AQP4). They form orthogonal arrays of particles (OAPs), which consist of AQP4 isoform M1 and M23. Under pathologic conditions, AQP4 is distributed over the whole cell and no or only small OAPs are found. From cell culture experiments, it is known that cells transfected only with AQP4-M1 do not form OAPs or only small ones. We hypothesized that in astrocytomas the situation may be comparable to the in vitro experiments expecting an upregulation of AQP4-M1. Quantitative Real-time PCR (qRT-PCR) of different graded astrocytomas revealed an upregulation of both isoforms AQP4 M1 and M23 in all astrocytomas investigated. In freeze fracture replicas of low-grade malignancy astrocytomas, more OAPs than in high-grade malignancy astrocytomas were found. In vitro, cultured glioma cells did not express AQP4, whereas healthy astrocytes revealed a slight upregulation of both isoforms and only a few OAPs in freeze fracture analysis. Taken together, we found a correlation between the decrease of OAPs and increasing grade of malignancy of astrocytomas but this was not consistent with an upregulation of AQP4-M1 in relation to AQP4 M23. 相似文献
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