7-Hydroxystaurosporine (UCN-01) induces apoptosis in human colon carcinoma and leukemia cells independently of p53

7-hydroxystaurosporine (UCN-01) is a more selective protein kinase C inhibitor than staurosporine. UCN-01 exhibits antitumor activity in experimental tumor models and is presently in clinical trials. Our study reveals that human myeloblastic leukemia HL60 and K562 and colon carcinoma HT29 cells undergo internucleosomal DNA fragmentation and morphological changes characteristic of apoptosis after UCN-01 treatment. These three cell lines lack functional p53, and K562 and HT29 cells are usually resistant to apoptosis. DNA fragmentation in HT29 and K562 cells occurred after 1 day of treatment while it took less than 4 h in HL60 cells. Cycloheximide prevented UCN-01-induced DNA fragmentation in HT-29 cells, but not in HL60 and K562 cells, suggesting that macromolecular synthesis is selectively required for apoptotic DNA fragmentation in HT29 cells. UCN-01-induced DNA fragmentation was preceded by activation of cyclin B1/cdc2 kinase. Further studies in HL60 cells showed that UCN-01-induced apoptosis was associated with degradation of CPP32, PARP, and lamin B and that the inhibitor of caspases (ICE/CED-3 cysteine proteases), Z-VAD-FMK, and the serine protease inhibitor, DCI, protected HL60 cells from UCN-01-induced DNA fragmentation. However, only DCI and TPCK, but not Z-VAD-FMK, inhibited DNA fragmentation in the HL60 cell-free system, suggesting that serine protease(s) may play a role in the execution phase of apoptosis in HL60 cells treated with UCN-01. Z-VAD-FMK and DCI also inhibited apoptosis in HT29 cells. These data demonstrate that the protein kinase C inhibitor and antitumor agent, UCN-01 is a potent apoptosis inducer in cell lines that are usually resistant to apoptosis and lack p53 and that caspases and probably serine proteases are activated during UCN-01-induced apoptosis.     

广义杠杆规则及其在配方计算中的应用

作者将相图中的一维杠杆规则推广至多元配料系统而成为广义杠杆规则，并利用这一规则对配方计算中解的可行域问题进行了深入的讨论，给出了几何解释及工艺诠释，对配方设计有一定的指导作用。     

汽车密封型材低摩擦系数涂料的研制

在提高汽车门窗活动部件的密封性能上，国外厂家提出采用先进的低摩擦系数涂料以代替目前使用的静电植绒，此课题在国内尚属新的领域。本文介绍对此进行的研究情况及经试验取得的结果。     

薄Si膜对基底表面粗糙度的影响

利用ＺＹＧＯ光学干涉测量仪，散射积分测量法观测了光学元件表面均方根粗糙度．详细分析了薄Ｓｉ膜对基底均方根粗糙度的影响，由此认为薄膜并不总是复制基底表面的粗糙度，结果出现了薄膜降低表面粗糙度的现象．提出了一定厚度范围的薄Ｓｉ膜的表面粗糙度存在着一个稳定值的新设想．     

Oxygen absorption kinetics in YBa2Cu3Ox

The oxygen absorption process in highly aligned YBa₂Cu₃O_x samples in air and in pure oxygen was studied at several constant temperatures. The process can be expressed in two steps, a chemical reaction controlled step and a diffusion controlled step. The kinetic equations are as follows respectively:

(1 − a_t) ln(1 − a_t) + a_t = k_Dt

The activation energy was calculated using an Arrhenius equation. The technical conditions of heat treatment for oxygen absorption of highly aligned YBa₂Cu₃O_x crystals are discussed.     

柔性冗余度机器人残余振动主动控制

研究柔性冗余度机器人的残余振动主动控制问题，设计了具有压电作动器与应变传感器的机敏杆件，建立了受控系统的状态空间表达式，采用独立模态空间控制理论设计LQR状态反馈控制器，并基于对偶原理设计了具有指定收敛特性的Luenberger全维状态观测器，最后，以平面3R柔性冗余度机器人为例进行了计算机仿真，结果表明，采用这种主动控制方法可以显著改善柔性冗余度机器人的动力学品质。     

一类广义耦合的KdV方程组的整体吸引子

利用Sobolev插值不等式和关于时间t的先验估计，研究了一类广义耦合的具有周期初值问题的KdV方程，得到了整体解和整体吸引子的存在性。     

面向对象中文窗口开发环境：C—VISION

Ｃ－ＶＩＳＩＯＮ中文窗口系统综合采用了面向对象与窗口环境设计的程序方法，彻底更新了在微机上进行中文窗口设计的编程思想，同时作为一个基础系统，为软件设计节约了大量的人力和时间，实现了微机程序的共享。     

低温粉碎系统中旋风分离器设计

旋风分离器是一种应用离心力进行粉体分离的设备。低温粉碎系统应用它分离粉体并起集粉作用。它的效率决定了物质回收率。本文从离心加速度与重力加速度的比值，筒体的高低，筒体直径，进口速度叙述对分离器效率的影响。着重叙述筒体直径确定方法，以及我们设计造长筒形旋风分离器的具体结构和令人满意的试验结果。     

Topology and Stability of Trivalent Polyhedral Clusters

An algorithm to generate the molecular graphs of the trivalent clusters is presented. All the polyhedral trivalent cages with 4-16 vertices are enumerated, with particular attention being given to their symmetry, spectra and stability. The relative stability of isomers is not decided solely by their symmetry. A new kind of spectral phenomenon is reported. This is the occurrence of graphs having the same HOMO-LUMO gap, the same E and the same even-order moments. We refer to it as 'pseudo-isospectrality'.