Oxidative activities of heterologously expressed CYP107B1 and CYP105D1 in whole-cell biotransformation using Streptomyces lividans TK24

Cytochrome P450 enzymes are a major class of biocatalysts related to the oxidative metabolism of many drugs, assisted by electron transfer partners. The functional expression of the P450 gene in a heterologous host will lead to efficient biotransformation and biodegradation, which are useful in pharmaceutical improvement or environmental cleanup. The soluble cytochrome P450 monooxygenase systems CYP105D1 and CYP107B1 involved in the biotransformation of some xenobiotics, such as secondary metabolites or environmental pollutants, were expressed in Streptomyces lividans TK24 with the Streptomyces expression vector pIJ6021. In whole-cell biotransformation assay using these recombinant strains, the oxidative dealkylation of 7-ethoxycoumarin was detected without any foreign redox partners in the case of CYP107B1, while the activity of CYP105D1 was not monitored until this gene was coexpressed with the ferredoxin gene located downstream of the CYP105D1 gene, and the ferredoxin reductase gene SCF 15.02 from Streptomyces coelicolor A3(II). This result suggests that CYP107B1 is capable of utilizing an endogenous electron transfer partner from the host but not CYP105D1, and that CYP105D1 is complemented by some redox partner imported from closely related strains.     

Activities of liver mitochondrial and peroxisomal fatty acid oxidation enzymes in rats fedtrans fat

The effect oftrans fat on the activities of liver mitochondrial and peroxisomal fatty acid oxidation enzymes was examined in various strains of rats. When Wistar and Sprague-Dawley rats were fed for 30 days diets containing either olive oil or partially hydrogenated corn oil as a source ofcis-ortrans-octadecenoate, respectively, the activities of various enzymes of mitochondrial and peroxisomal β-oxidation measured withcis- andtrans-9-octadecenoic acid as substratese showed little dietary fatdependent change. In Fischer 344 rats, feedingtrans fat for 15 mo increased only moderately various enzymes of β-oxidation except for carnitine acyltransferase. The rate of mitochondrial ketogenesis and the activity of carnitine acyltransferase measured withtrans-9-octadecenoic acid as a substrate were about half those with thecis-counterpart. Peroxisomes oxidizedtrans-9-octadecenoyl-CoA at a rate comparable to thecis-counterpart. It was concluded from this study and previous ones that the difference in the geometry of dietary fatty acid had only a marginal effect in modulating the hepatic fatty acid oxidation system, in spite of marked differences in the metabolic behavior ofcis-andtrans fatty acid in cell-free preparations and perfused liver.     

Volumetric data exploration using interval volume

A new type of geometric model called Interval volume for volumetric data exploration is presented. An interval volume represents a three dimensional subvolume for which the associate scalar values lie within a user specified interval, and provides one of the promising approaches to solid fitting, which is an extended concept of traditional surface fitting. A well known isosurfacing algorithm called Marching Cubes is extended to obtain a solid fitting algorithm, which extracts from a given volumetric data set a high resolution, polyhedral solid data structure of an interval volume. Branch-on-Need Octree is used as an auxiliary data structure to accelerate the extraction process. A variety of interval volume rendering methods and principal related operations, including measurements and focusing, are also presented. The effectiveness of measurement coupled visualization capabilities of the presented approach is demonstrated by application to visualizing a four dimensional simulated data from atomic collision research     

Thermal stability and mechanical properties of yttria-doped tetragonal zirconia polycrystals with dispersed alumina and silicon carbide particles

Yttria-doped tetragonal zirconia polycrystals in which were dispersed various amounts of Al₂O₃ and SiC particles were sintered at 1500° C for 3 h, and the mechanical properties and the thermal stability of the sintered bodies were evaluated. Dispersion of Al₂O₃ caused no significant effect on sinterability, and increased the hardness and elasticity of the composites. Dispersion of SiC particles decreased the relative density and the grain size of composites. Elasticity and hardness increased by dispersing less than 10 vol% SiC, but decreased above 10 vol% SiC due to the decrease of relative density. Dispersion of both Al₂O₃ and SiC particles slightly increased the fracture toughness of ZrO₂-3 mol% Y₂O₃ ceramics but significantly decreased that of ZrO₂-2 mol% Y₂O₃ ceramics. The rate of the tetragonal-to-monoclinic phase transformation decreased by dispersing both Al₂O₃ and SiC particles. The transformation depth increased rapidly and then slowly with increasing the annealing time. The rate of increase in the transformation depth greatly decreased by dispersing Al₂O₃ particles.     

Examination of the destructive forces in the chernobyl accident based on NSRR experiments

The possible causes of the destruction of the Chernobyl reactor core were examined by making use of the Nuclear Safety Research Reactor (NSRR) experimental results concerning the destructive forces generated by a fuel failure. A complementary experiment with Chernobyl reactor conditions was performed in order to observe the fuel failure behavior and the resultant vessel pressure rise, etc. Also, generation of hydrogen from the fuel rod cladding and the consequent system pressure rise were estimated based on the experiments.These examinations led to the conclusion that the most probable cause of the core pressure tube rupture in the accident was a static pressure rise due to rapid energy release from fragmented fuel. Other phenomena such as the hydrogen generation and molten fuel contact to the tube wall might have contributed to the tube rupture. The water hammer force is also estimated to have been large enough to break tubes even using conservative assumptions.     

A 20-Gb/s flip-flop circuit using direct-coupled FET logic

A new type of direct-coupled FET logic (DCFL) flip-flop called the memory cell type flip-flop (MCFF) is presented. The MCFF operates faster than conventional DCFL flip-flops and enhances the DCFL's advantages, such as low power consumption and high packing density. A D-flip-flop IC and a 1/8 divider IC were developed using the MCFF. These ICs were fabricated using 0.2-μm-gate pseudomorphic inverted HEMTs. The D-flip-flop IC is confirmed to operate up to 20 Gb/s. The 1/8 divider is toggled up to a maximum frequency of 25 GHz. These results prove that the MCFF enables DCFL circuits applicable not only to large-scale integration but to small-scale and medium-scale integration operating up to 20 Gb/s as well     

Effects of dietaryTrans-fat on biliary and fecal steroid excretion and serum lipoproteins in rats

Rats were fed cholesterol-free or cholesterol-enriched diets containing olive oil or partially hydrogenated corn oil at the 10% level for ca. 30 days (c-18∶1, 77.0% in the former diet andc-18∶1, 24.7% andt-18∶1, 42.5% in the latter). The linoleic acid content of these fat diets was made equivalent (1.7 energy%). After feeding cholesterol-free diets,trans fat compared tocis fat showed(a) no untoward effects on growth parameters, (b) a reduction of serum cholesterol levels without influencing concentrations of serum apolipoproteins A-I, B and E, (c) no effects on the bile flow and the concentration of biliary cholesterol and bile acids, (d) an increasing trend of fecal excretion of neutral and acidic steroids, both in terms of mg/day and mg/g feces, and (e) rather equivocal change in the composition of fecal, but not biliary steroids. Similar response patterns were also observed when cholesterol-enriched diets were fed except for a decrease in serum apo B and an ineffectiveness to increase fecal acidic steroids. Together with the results obtained from experiments simultaneously performed with safflower oil and completely hydrogenated corn oil, it seems that the steroid metabolism can be specificallymodified by the geometry of dietary fats.     

Effects of contact‐dehydrating sheets on preference for fried food and deterioration of frying oil

The purpose of this study was to investigate the effects of contact‐dehydrating sheets on preferences for fried food and the deterioration of frying oil. Mackerel or chicken breast were dehydrated by wrapping with the contact‐dehydrating sheets at 4°C for 1.5 h and subsequently fried at 180°C for 3 min; those were referred to as the dehydrated samples. The same fried materials not wrapped with the contact‐dehydrating sheets were referred to as the control. The frying procedure was repeated 15 times under the above conditions and the amount of frying oil was adjusted to 1000 g by addition of new oil each time. Sensory analysis showed that the dehydrated samples were preferred to the control. Acidic values, anisidine values and ΔE values of the frying oil used for the dehydrated mackerel were significantly lower than for that used for preparing the control. These results indicate that the use of contact‐dehydrating sheets for the dehydration of the frying materials was effective in both significantly increased preference for the fried food and the retardation of the deterioration of the frying oil. Copyright © 2004 Society of Chemical Industry