全文获取类型
收费全文 | 375篇 |
免费 | 29篇 |
学科分类
工业技术 | 404篇 |
出版年
2023年 | 7篇 |
2022年 | 8篇 |
2021年 | 41篇 |
2020年 | 16篇 |
2019年 | 7篇 |
2018年 | 14篇 |
2017年 | 18篇 |
2016年 | 20篇 |
2015年 | 19篇 |
2014年 | 26篇 |
2013年 | 25篇 |
2012年 | 19篇 |
2011年 | 21篇 |
2010年 | 17篇 |
2009年 | 25篇 |
2008年 | 12篇 |
2007年 | 12篇 |
2006年 | 12篇 |
2005年 | 14篇 |
2004年 | 10篇 |
2003年 | 8篇 |
2002年 | 7篇 |
2001年 | 6篇 |
2000年 | 7篇 |
1999年 | 5篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1995年 | 5篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
排序方式: 共有404条查询结果,搜索用时 15 毫秒
31.
Sabrina Burattini Paola Ferri Michela Battistelli Alessandra D'emilio Laura Biagiotti Piero Sestili Marco B. L. Rocchi Elisabetta Falcieri 《Microscopy research and technique》2009,72(12):913-923
A common pattern of apoptotic death is DNA cleavage, initially producing large fragments (50 kbp), followed by the production of nucleosomic/oligonucleosomic fragments. Nevertheless, apoptosis without DNA fragmentation, at least of the nucleosomic type, has been reported. To investigate the spatial relationship between DNA cleavage and chromatin condensation, we applied the TUNEL technique to the ultrastructural analysis of apoptotic cells. A modified method, utilizing a gold‐conjugated antidigoxigenin antibody, was carried out on U937 versus Molt‐4 cells, both exposed to UVB radiation or staurosporine treatment. Gold particle density in the different domains of apoptotic cells was evaluated by a four‐way ANOVA test. Gold labelling was more strongly localised in condensed chromatin than in the diffuse chromatin. U937 cells, which evidenced in vitro oligonucleosomic fragmentation after both UVB and staurosporine treatments, revealed a significantly higher gold particle density, when compared with Molt‐4, which did not show, on the other hand, oligonucleosomic cleavage even in the presence of ≤50 kbp cleavage. Thus, a correlation between DNA fragment sizes and gold particle density appears. TUNEL applied to electron microscopy is an effective approach to study the relationship between apoptotic chromatin condensation and DNA cleavage. Both these events indeed appear in the apoptotic nucleus, but their reciprocal correlation is still greatly unknown. Microsc. Res. Tech. 2009. © 2009 Wiley‐Liss, Inc. 相似文献
32.
Michela Montesi Author Vitae 《Journal of Systems and Software》2008,81(10):1694-1714
The software engineering (SE) community has recently recognized that the field lacks well-established research paradigms and clear guidance on how to write good research reports. With no comprehensive guide to the different article types in the field, article writing and reviewing heavily depends on the expertise and the understanding of the individual SE actors.In this work, we classify and describe the article types published in SE with an emphasis on what is required for publication in journals and conference proceedings. Theoretically, we consider article types as genres, because we assume that each type of article has a specific function and a particular communicative purpose within the community, which the members of the community can recognize. We draw on written sources available, i.e. the instructions to authors/reviewers of major SE journals, the calls for papers of major SE conferences, and previous research published on the topic.Despite the fragmentation and limitations of the sources studied, we are able to propose a classification of different SE article types. Such classification helps in guiding the reader through the SE literature, and in making the researcher reflect on directions for improvements. 相似文献
33.
Several animal studies have shown that there is a positive correlation between locomotor activity in response to a novel environment and acquisition of drug self-administration behavior. This finding led to the assumption that animals with heightened reactivity to novel environments are more sensitive to the rewarding effects of drugs compared with animals with reduced reactivity. But are these individuals really more responsive to drugs, or could they have enhanced sensitivity to rewards in general or even simply be better learners? In the previous issue of this journal, J. M. Mitchell, C. L. Cunningham, and G. P. Mark (2005) (see record 2005-03585-010), investigated these important matters. They reported that the locomotor response to a novel environment does not predict responding for cocaine but reflects overall differences in the ability to learn operant tasks. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
34.
Gallagher Michela; Meagher Mary W.; Bostock Elizabeth 《Canadian Metallurgical Quarterly》1987,101(3):315
Rabbits were exposed to an auditory stimulus that was later used as a conditioned stimulus (CS). Administration of the agonist levorphanol into the medial septal region either preceding or immediately following CS preexposure sessions attenuated latent inhibition of the conditioned response. This effect was observed in animals that showed no change in long-term retention of habituation. Administration of levorphanol into the central nucleus of the amygdala, a sensitive site for opiate manipulations on aversive conditioning tasks, were ineffective. Further analysis of the effect obtained within the septal region revealed pharmacological specificity. The effect of posttraining levorphanol was also time dependent and exhibited neuroanatomical specificity. Posttraining opiate antagonist administration into the medial septum produced a modest augmentation of latent inhibition. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
35.
Modeling the color variation due to an illuminant change is an important task for many computer vision applications, like color constancy, object recognition, shadow removal and image restoration. The von Kries diagonal model and Wien’s law are widely assumed by many algorithms for solving these problems. In this work we combine these two hypotheses and we show that under Wien’s law, the parameters of the von Kries model are related to each other through the color temperatures of the illuminants and the spectral sensitivities of the acquisition device. Based on this result, we provide a method for estimating some camera cues that are used to compute the illuminant invariant intrinsic image proposed by Finlayson and others. This is obtained by projecting the log-chromaticities of an input color image onto a vector depending only on the spectral cues of the acquisition device. 相似文献
36.
Michela Antonelli Marco Cococcioni Beatrice Lazzerini Francesco Marcelloni 《Pattern Analysis & Applications》2011,14(3):295-310
We adopted decision fusion techniques to develop a computer-aided detection (CAD) system for automatic detection of pulmonary
nodules in low-dose CT images. Two distinct phases, aimed, respectively, at detecting volumes of interests (VOIs) within the
CT scan, and at classifying VOIs into nodules and non-nodules, were considered. Three algorithms, namely thresholding, region
growing and robust fuzzy clustering, were used as VOI detectors. For the classification phase, we built multi-classifier systems,
which aggregate the decisions of three statistical classifiers, a neural network and a decision tree. Finally, the receiver
operating characteristic convex hull method was used to build the final classifier, which results to be the aggregation of
the best local behaviors of both classifiers and combiners. All the CAD modules were tested on CT scans analyzed by two expert
radiologists. In the experiments, we achieved a sensitivity of 92.5% against a specificity of 83.5%. 相似文献
37.
38.
The ninth edition of the biennial conference series “Nexus: Relationships Between Architecture and Mathematics” took place at the Politecnico di Milan in June 2012. There are many connections between architecture and mathematics: mathematic principles may be used as a basis for an architectural design, or as a tool for analyzing an existing monument; architecture may be a concrete expression of mathematical ideas, becoming, in a sense, “visual mathematics”. The purpose of the Nexus conference series is to bring together all those working with ideas related to both architecture and mathematics, and to allow researchers to exchange ideas first-hand. Papers presented at the conference are published in the Nexus Network Journal, providing a permanent archive of studies in architecture and mathematics, and in 2012 a special volume of papers from the Ph.D. Day and the poster session was published. 相似文献
39.
40.
Ioannis Pratikakis Michela Spagnuolo Theoharis Theoharis Remco Veltkamp 《The Visual computer》2010,26(10):1243-1243