Analysis of fumonisins in corn-based food by liquid chromatography with fluorescence and mass spectrometry detectors

The presented procedure involves an extraction with methanol–water, centrifugation and cleanup with immunoaffinity columns. A comparison study between fluorescence detector, mass spectrometry, and tandem mass spectrometry with a triple quadrupole (QqQ) analyzer using an electrospray ionisation interface for the determination of fumonisin B₁ and B₂ in corn-based products has been performed.     

A tool to analyze robust stability for model predictive controllers

A strategy based on Nonlinear Programming (NLP) sensitivity is developed to establish stability bounds on the plant/model mismatch for a class of optimization-based Model Predictive Control (MPC) algorithms. By extending well-known nominal stability properties for these controllers, we derive a sufficient condition for robust stability of these controllers. This condition can also be used to assess the extent of model mismatch that can be tolerated to guarantee robust stability. In this derivation we deal with MPC controllers with final time constraints or infinite time horizons. Also for this initial study we concentrate only on discrete time systems and unconstrained state feedback control laws with all of the states measured. To illustrate this approach we give two examples: a linear first-order dynamic system and a nonlinear SISO system involving a first order reaction. ©     

Levels of ochratoxin A in wheat and maize bread from the central zone of Portugal

Ochratoxigenic fungi are natural contaminants of cereal and the produced toxins are harmful to humans and animals. Ochratoxin A (OTA) is among the most important mycotoxins, and the International Agency for Research on Cancer (IARC) classifies it as possibly carcinogenic to humans (group 2B). A total of 61 samples of bread from the central zone of Portugal were analysed for OTA by liquid chromatography (LC) with fluorescence detection (FD). For confirmation two procedures were applied, methyl ester derivatization with boron trifluoride-methanol and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI/MS/MS). As far as we know, this is the first report where on-line LC/electrospray ionization (ESI) tandem mass spectrometry (MS/MS) was used for OTA analysis in bread. Limits of detection (LOD) and quantification (LOQ) were 0.015 and 0.03 ng/g, using LC-FD, and 0.03 and 0.09 ng/g by LC-MS/MS. The incidence of OTA was 12.9% and 70.0% for wheat and maize bread, respectively. The highest OTA levels were obtained for maize bread, having one sample exceeded the European maximum limit established for OTA in cereal products. The estimate daily intake (EDI) was below the tolerable daily intake.     

Antibiotic resistance and tolerance to simulated gastrointestinal conditions of eight hemolytic Bacillus pumilus isolated from pulque,a traditional Mexican beverage

Food Science and Biotechnology - Traditional fermented sap beverages from two provinces of Mexico were studied for the isolation of pathogenic bacteria from these beverages. Eight strains of...     

Radiation resistivity in silica optical fibers

Hydrogen treatment of optical fiber waveguides with pure silica cores and boron, fluorine codoped silica claddings was found to effectively reduce the y-ray induced loss increases in visible wavelength region. Germanium-doped core fibers free from metal impurities had good resistance against y-rays. The induced losses were shown to be dependent on the dose rate, a similar behavior to that of pure silica core fibers. Metal impurities such as phosphorus or alkalis added to germanium doped silica core were found to change the glass structure significantly, producing the precursors of a defect center related to the y-ray-induced loss and increasing sensitivity to y-irradiation     

Multi-robot olfactory search in structured environments

This paper presents a cooperative distributed approach for searching odor sources in unknown structured environments with multiple mobile robots. While searching and exploring the environment, the robots independently generate on-line local topological maps and by sharing them with each other they construct a global map. The proposed method is a decentralized frontier based algorithm enhanced by a cost/utility evaluation function that considers the odor concentration and airflow at each frontier. Therefore, frontiers with higher probability of containing an odor source will be searched and explored first. The method also improves path planning of the robots for the exploration process by presenting a priority policy. Since there is no global positioning system and each robot has its own coordinate reference system for its localization, this paper uses topological graph matching techniques for map merging. The proposed method was tested in both simulation and real world environments with different number of robots and different scenarios. The search time, exploration time, complexity of the environment and number of double-visited map nodes were investigated in the tests. The experimental results validate the functionality of the method in different configurations.