全文获取类型
收费全文 | 402篇 |
免费 | 21篇 |
学科分类
工业技术 | 423篇 |
出版年
2023年 | 7篇 |
2022年 | 8篇 |
2021年 | 63篇 |
2020年 | 25篇 |
2019年 | 15篇 |
2018年 | 21篇 |
2017年 | 18篇 |
2016年 | 19篇 |
2015年 | 21篇 |
2014年 | 17篇 |
2013年 | 33篇 |
2012年 | 25篇 |
2011年 | 43篇 |
2010年 | 21篇 |
2009年 | 15篇 |
2008年 | 21篇 |
2007年 | 16篇 |
2006年 | 9篇 |
2005年 | 5篇 |
2004年 | 4篇 |
2003年 | 4篇 |
2002年 | 3篇 |
2001年 | 2篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 1篇 |
排序方式: 共有423条查询结果,搜索用时 15 毫秒
71.
Muthuri Robert Capecchi Sara Sulis Emilio Amantea Ilaria Angela Boella Guido 《Information Systems and E-Business Management》2022,20(1):27-55
Information Systems and e-Business Management - Companies need to be able to demonstrate compliance with rules and regulations, especially start-ups who typically do not have the legal expertise to... 相似文献
72.
We report the use of superresolution fluorescence microscopy for studying the nanoscale distribution of protein colocalization in living mammalian cells. Nanoscale imaging is attained both by a targeted and a stochastic fluorescence on-off switching superresolution method, namely by stimulated emission depletion (STED) and ground state depletion microscopy followed by individual molecular return (GSDIM), respectively. Analysis of protein colocalization is performed by bimolecular fluorescence complementation (BiFC). Specifically, a nonfluorescent fragment of the yellow fluorescent protein Citrine is fused to tubulin while a counterpart nonfluorescent fragment is fused to the microtubulin-associated protein MAP2 such that fluorescence is reconstituted on contact of the fragment-carrying proteins. Images with resolution down to 65 nm prove a powerful new way for studying protein colocalization in living cells at the nanoscale. 相似文献
73.
Quinone‐Fused Pyrazoles through 1,3‐Dipolar Cycloadditions: Synthesis of Tricyclic Scaffolds and in vitro Cytotoxic Activity Evaluation on Glioblastoma Cancer Cells 下载免费PDF全文
Giulio Bertuzzi Simone Crotti Pierpaolo Calandro Bianca Flavia Bonini Ilaria Monaco Erica Locatelli Mariafrancesca Fochi Paolo Zani Elena Strocchi Prof. Andrea Mazzanti Dr. Mario Chiariello Prof. Mauro Comes Franchini 《ChemMedChem》2018,13(17):1744-1750
A novel and straightforward synthesis of highly substituted isoquinoline‐5,8‐dione fused tricyclic pyrazoles is reported. The key step of the synthetic sequence is a regioselective, Ag2CO3 promoted, 1,3‐dipolar cycloaddition of C‐heteroaryl‐N‐aryl nitrilimines and substituted isoquinoline‐5,8‐diones. The broad functional group tolerability and mild reaction conditions were found to be suitable for the preparation of a small library of compounds. These scaffolds were designed to interact with multiple biological residues, and two of them, after brief synthetic elaborations, were analyzed by molecular docking studies as potential anticancer drugs. In vitro studies confirmed the potent anticancer effects, showing promising IC50 values as low as 2.5 μm against three different glioblastoma cell lines. Their cytotoxic activity was finally positively correlated to their ability to inhibit PI3K/mTOR kinases, which are responsible for the regulation of diverse cellular processes in human cancer cells. 相似文献
74.
Palmieri A Pezzetti F Brunelli G Zollino I Lo Muzio L Martinelli M Scapoli L Arlotti M Masiero E Carinci F 《Journal of materials science. Materials in medicine》2008,19(6):2471-2476
Zirconium oxide (ZO) has outstanding mechanical properties, high biocompatibility and high resistance to scratching. Since
dental implants are made with ZO and the genetic effects of ZO on osteoblasts are incompletely understood, we used microRNA
microarray techniques to investigate the translation process in osteoblasts exposed to ZO. By using miRNA microarrays containing
329 probes designed from Human miRNA sequences, we identified in osteoblast-like cells line (MG-63) cultured on ZO disks several
miRNA whose expression was significantly modified. The most notable regulated genes acting on osteoblasts are: NOG, SHOX,
IGF1, BMP1 and FGFR1. The data reported below represent the first study on translation regulation in osteoblasts exposed to
zirconium and one in which the effect of ZO on bone formation has been detected. 相似文献
75.
76.
77.
78.
Silvio Borrelli Luca De Nicola Ilaria De Gregorio Lucio Polese Luigi Pennino Claudia Elefante Alessandro Carbone Tiziana Rappa Roberto Minutolo Carlo Garofalo 《International journal of molecular sciences》2021,22(23)
Sodium overload is common in end-stage kidney disease (ESKD) and is associated with increased cardiovascular mortality that is traditionally considered a result of extracellular volume expansion. Recently, sodium storage was detected by Na23 magnetic resonance imaging in the interstitial tissue of the skin and other tissues. This amount of sodium is osmotically active, regulated by immune cells and the lymphatic system, escapes renal control, and, more importantly, is associated with salt-sensitive hypertension. In chronic kidney disease, the interstitial sodium storage increases as the glomerular filtration rate declines and is related to cardiovascular damage, regardless of the fluid overload. This sodium accumulation in the interstitial tissues becomes more significant in ESKD, especially in older and African American patients. The possible negative effects of interstitial sodium are still under study, though a higher sodium intake might induce abnormal structural and functional changes in the peritoneal wall. Interestingly, sodium stored in the interstial tissue is not unmodifiable, since it is removable by dialysis. Nevertheless, the sodium removal by peritoneal dialysis (PD) remains challenging, and new PD solutions are desirable. In this narrative review, we carried out an update on the pathophysiological mechanisms of volume-independent sodium toxicity and possible future strategies to improve sodium removal by PD. 相似文献
79.
Bernd M. Linke Thomas Gerber Ansgar Hatscher Ilaria Salvatori Iñigo Aranguren Maribel Arribas 《Metallurgical and Materials Transactions A》2018,49(1):54-65
Based on 22MnB5 hot stamping steel, three model alloys containing 0.5, 0.8, and 1.5 wt pct Si were produced, heat treated by quenching and partitioning (Q&P), and characterized. Aided by DICTRA calculations, the thermal Q&P cycles were designed to fit into industrial hot stamping by keeping partitioning times ≤ 30 seconds. As expected, Si increased the amount of retained austenite (RA) stabilized after final cooling. However, for the intermediate Si alloy the heat treatment exerted a particularly pronounced influence with an RA content three times as high for the one-step process compared to the two-step process. It appeared that 0.8 wt pct Si sufficed to suppress direct cementite formation from within martensite laths but did not sufficiently stabilize carbon-soaked RA at higher temperatures. Tensile and bending tests showed strongly diverging effects of austenite on ductility. Total elongation improved consistently with increasing RA content independently from its carbon content. In contrast, the bending angle was not impacted by high-carbon RA but deteriorated almost linearly with the amount of low-carbon RA. 相似文献
80.
Enrico Capuzzi Alice Caldiroli Martina Capellazzi Ilaria Tagliabue Matteo Marcatili Fabrizia Colmegna Massimo Clerici Massimiliano Buoli Antonios Dakanalis 《International journal of molecular sciences》2021,22(17)
Esketamine (ESK) has been approved as a rapid-acting intranasal treatment for treatment-resistant depression (TRD). Although existing studies have investigated the efficacy of ESK in the 4-week induction phase, our knowledge about long-term ESK efficacy remains poor. The aim of this systematic review was to summarize the available data on long-term ESK efficacy for TRD. A systematic search was performed including articles in English, up to 31 March 2021. The search found 7 relevant studies, involving 1024 adult TRD patients. Continuing treatment with ESK after the 4-week induction phase may be associated with stable efficacy in relapse prevention among TRD patients. Conversely, the long-term antidepressant effectiveness upon discontinuation of ESK might be limited, although data from three studies had a moderate to high risk of bias. Overall, the results on the effectiveness of this compound in the long term are mixed. According to our findings, ESK treatment should be continued following the induction phase to reach a stable efficacy in relapse prevention, while the long-term antidepressant and anti-suicidal effects of ESK after discontinuation are inconsistent. Currently, the level of proof of ESK efficacy in long-term TRD treatment remains low and more RCTs with larger sample sizes and active comparators are needed. 相似文献