Effects of milk high pressure homogenization on biogenic amine accumulation during ripening of ovine and bovine Italian cheeses

The aim of this work was to evaluate the biogenic amine (BA) content during the ripening of both bovine and ovine cheeses obtained using milk subjected to a homogenization treatment at 100 MPa before cheese-making. The data obtained were compared with those from cheeses produced by the same milks without any treatment or thermized. The results showed that both microbial ecology and BA concentrations of cheeses during ripening were significantly influenced by the type of milk used for cheese-making and by the treatment applied to the raw materials. In particular, the microbial counts found in Caciotta indicated that the high pressure homogenization (HPH) of milk significantly reduced the presence of the yeasts, Micrococcaceae and lactobacilli at the end of ripening. On the other hand, the HPH treatment of milk favoured the proliferation of yeasts in ovine cheese. Moreover, the ovine cheeses were characterized by a remarkably higher accumulation of BA than bovine cheeses. However, the HPH treatment of milk was able to drastically reduce the biogenic amine concentrations in both cheese typologies at the end of ripening.     

Potent,Metabolically Stable 2‐Alkyl‐8‐(2H‐1,2,3‐triazol‐2‐yl)‐9H‐adenines as Adenosine A2A Receptor Ligands

Inhibition of adenosine A_2A receptors has been shown to elicit a therapeutic response in preclinical animal models of Parkinson’s disease (PD). We previously identified the triazolo‐9H‐purine, ST1535, as a potent A_2AR antagonist. Studies revealed that ST1535 is extensively hydroxylated at the ω‐1 position of the butyl side chain. Here, we describe the synthesis and evaluation of derivatives in which the ω‐1 position has been substituted (F, Me, OH) in order to block metabolism. The stability of the compounds was evaluated in human liver microsomes (HLM), and the affinity for A_2AR was determined. Two compounds, (2‐(3,3‐dimethylbutyl)‐9‐methyl‐8‐(2H‐1,2,3‐triazol‐2‐yl)‐9H‐purin‐6‐amine ( 3 b ) and 4‐(6‐amino‐9‐methyl‐8‐(2H‐1,2,3‐triazol‐2‐yl)‐9H‐purin‐2‐yl)‐2‐methylbutan‐2‐ol ( 3 c ), exhibited good affinity against A_2AR (K_i=0.4 nM and 2 nM , respectively) and high in vitro metabolic stability (89.5 % and 95.3 % recovery, respectively, after incubation with HLM for two hours).     

Docosahexaenoic Acid Levels in Blood and Metabolic Syndrome in Obese Children: Is There a Link?

Prevalence of metabolic syndrome is increasing in the pediatric population. Considering the different existing criteria to define metabolic syndrome, the use of the International Diabetes Federation (IDF) criteria has been suggested in children. Docosahexaenoic acid (DHA) has been associated with beneficial effects on health. The evidence about the relationship of DHA status in blood and components of the metabolic syndrome is unclear. This review discusses the possible association between DHA content in plasma and erythrocytes and components of the metabolic syndrome included in the IDF criteria (obesity, alteration of glucose metabolism, blood lipid profile, and blood pressure) and non-alcoholic fatty liver disease in obese children. The current evidence is inconsistent and no definitive conclusion can be drawn in the pediatric population. Well-designed longitudinal and powered trials need to clarify the possible association between blood DHA status and metabolic syndrome.     

Kuwanon‐L as a New Allosteric HIV‐1 Integrase Inhibitor: Molecular Modeling and Biological Evaluation

HIV‐1 integrase (IN) active site inhibitors are the latest class of drugs approved for HIV treatment. The selection of IN strand‐transfer drug‐resistant HIV strains in patients supports the development of new agents that are active as allosteric IN inhibitors. Here, a docking‐based virtual screening has been applied to a small library of natural ligands to identify new allosteric IN inhibitors that target the sucrose binding pocket. From theoretical studies, kuwanon‐L emerged as the most promising binder and was thus selected for biological studies. Biochemical studies showed that kuwanon‐L is able to inhibit the HIV‐1 IN catalytic activity in the absence and in the presence of LEDGF/p75 protein, the IN dimerization, and the IN/LEDGF binding. Kuwanon‐L also inhibited HIV‐1 replication in cell cultures. Overall, docking and biochemical results suggest that kuwanon‐L binds to an allosteric binding pocket and can be considered an attractive lead for the development of new allosteric IN antiviral agents.     

Reinforced concrete and masonry arch bridges in seismic areas: typical deficiencies and retrofitting strategies

In recent years, appraisal of the condition and rehabilitation of existing bridges has become an ongoing problem for bridge owners and administrators in all developed countries. Reliable methodologies are therefore needed in the assessment and retrofit design phases, to identify the vulnerability of each bridge class. The specific problems of common arch bridge types are discussed herein, for both reinforced concrete and masonry structures, proper interventions for their static and seismic retrofitting are illustrated and several examples of applications are provided. Retrofitting is usually coupled with functional refurbishment, according to a methodological approach that takes into account bridge characteristics, state of maintenance and functional requirements, and environmental aspects connected with repair and strengthening systems.     

An exergetic analysis of a membrane desalination system

In this paper, the exergetic analysis of a seawater membrane-based desalination plant has been carried out. The desalination plant has been described in detail, then the exergy of the various saline water streams has been determined and a comprehensive analysis towards the exergy distribution of the major process components has been conducted. The examination of the exergy losses throughout the plant revealed that exergy destruction was mainly due to pressure drops in the membrane modules, valves and brine lines. Moreover, 12.9% of the exergy input to the system was supplied by the heater. Therefore, the most reasonable way to reduce power input to the plant, thus improving its performance and cost, has been shown (i) to be replacing the valves on the reverse osmosis brine stream by an energy recovery system, and (ii) to have thermal energy available in the plant. With the identified technical changes, energy consumption decreased from 18.3 to 2.05 kWh/m³, resulting in an annual saving of 0.17$/m³.     

Layer‐by‐Layer All‐Inorganic Quantum‐Dot‐Based LEDs: A Simple Procedure with Robust Performance

A novel all‐inorganic electroluminescent device is demonstrated based on highly luminescent CdTe nanocrystals intercalated within a laminar hydrotalcite‐like structure. The laminar scaffold acts to both support and distribute the CdTe nanocrystals. The device is synthesized using simple wet chemical processes at room temperature in ambient conditions. It has high thermal stability, operating continuously up to 90 °C, and a maximum efficiency at J = 0.12 A cm^?2. The device is targeted at the automotive industry.     

Characteristics of double-crested cormorant colonies in the U.S. Great Lakes island landscape

The Great Lakes form the largest freshwater island system in the world and provide breeding habitat for a large proportion of the continental population of double-crested cormorants (Phalacrocorax auritus). Here, cormorants have a high profile due to conflicts with humans; by 2007, most active (64%) breeding sites in U.S. waters were managed. This study used data from the U.S. Great Lakes Colonial Waterbird Database and The Nature Conservancy's Great Lakes Island GIS database to identify important features of breeding sites in the U.S. Great Lakes and broaden understanding of cormorant presence at the island-landscape scale. Islands 0.5–10 ha were used more frequently than expected, and most sites had remoteness values of ≤ 3 km. Colony size was positively correlated with years occupied and large colonies (> 1000 pairs) developed primarily (95%) on island sites > 1.0 ha. Sites supporting large colonies were more remote than those supporting smaller colonies. Presence of other colonial waterbird species, especially Herring Gulls (Larus argentatus), also characterized cormorant sites. Islands used by cormorants comprised a small proportion (n = 90, 3%) of the U.S. Great Lakes island resource, and < 1% of the total island area. Certain characteristics of breeding sites (e.g., small islands, proximity to mainland) may increase negative attitudes about cormorants. To understand cormorant impacts to island resources (e.g., vegetation; other colonial waterbird species), we suggest cormorant presence in the Great Lakes be considered in the broader context of island science, conservation and known threats, and at a landscape scale.