Essential requirement of cytosolic phospholipase A2 for activation of the phagocyte NADPH oxidase

Arachidonic acid (AA) can trigger activation of the phagocyte NADPH oxidase in a cell-free assay. However, a role for AA in activation of the oxidase in intact cells has not been established, nor has the AA generating enzyme critical to this process been identified. The human myeloid cell line PLB-985 was transfected to express p85 cytosolic phospholipase A2 (cPLA2) antisense mRNA and stable clones were selected that lack detectable cPLA2. cPLA2-deficient PLB-985 cells differentiate similarly to control PLB-985 cells in response to retinoic acid or 1,25-dihydroxyvitamin D3, indicating that cPLA2 is not involved in the differentiation process. Neither cPLA2 nor stimulated [3H]AA release were detectable in differentiated cPLA2-deficient PLB-985 cells, demonstrating that cPLA2 is the major type of PLA2 activated in phagocytic-like cells. Despite the normal synthesis of NADPH oxidase subunits during differentiation of cPLA2-deficient PLB-985 cells, these cells fail to activate NADPH oxidase in response to a variety of soluble and particulate stimuli, but the addition of exogenous AA fully restores oxidase activity. This establishes an essential requirement of cPLA2-generated AA for activation of phagocyte NADPH oxidase.     

The stable topology for residuated lattices

The spectrum of a residuated lattice L is the set Spec(L) of all prime i-filters. It is well known that Spec(L) can be endowed with the spectral topology. The main scope of this paper is to introduce and study another topology on Spec(L),?the so called stable topology, which turns out to be coarser than the spectral one. With this and in view, we introduce the notions of pure i-filter for a residuated lattice and the notion of normal residuated lattice. So, we generalize to case of residuated lattice some results relative to MV-algebras (Belluce and Sessa in Quaest Math 23:269–277, 2000; Cavaccini et?al. in Math Japonica 45(2):303–310, 1997) or BL-algebras (Eslami and Haghani in Kybernetika 45:491–506, 2009; Leustean in Central Eur J Math 1(3): 382–397, 2003; Turunen and Sessa in Mult-Valued Log 6(1–2):229–249, 2001).     

Crystallization of poly(3‐hydroxybutyrate) by exfoliated graphite nanoplatelets

Poly(3‐hydroxybutyrate) (PHB) has been shown to be efficiently nucleated by exfoliated graphite nanoplatelets (xGnP). The nucleating effect of xGnP was investigated using differential scanning calorimetry, optical microscopy and atomic force microscopy. Nonisothermal crystallization of PHB from the melt required lower activation energies for PHB containing 1 wt % and 3 wt % xGnP (?214 and ?102 kJ/mol respectively) than for pure PHB (?60 kJ/mol). A kinetic study of the PHB/xGnP crystallization employing a modified form of the Avrami equation revealed that the presence of xGnP increased the PHB crystallization temperature, as well as the crystallization rates, and generated smaller and more numerous spherulites. Optical microscopy and atomic force microscopy confirmed the incorporation of xGnP into the lamellar structure of the PHB spherulites and provided insight into the influence of xGnP on spherulite size and lamellae thickness. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2007     

Forces required to jettison a simulated S92 passenger exit: Optimal helicopter underwater egress training techniques

This paper outlines the first known examination of the forces required to jettison a simulated exit used during helicopter underwater egress training (HUET). To capture the forces placed on the simulated cabin exit, a purpose build force plate was designed to replace an existing simulator exit used during HUET. A 25-point map was created to identify specific jettison forces required across the entire exit surface. Ten participants completed a total of 120 underwater egress sequences in–air and in-water from a normal flight and fully compressed crash attenuating seat position. The results indicate that the force required to jettison the simulated exit is significantly different in relationship to location on the exit surface. From the results, it can be concluded that helicopter underwater egress training protocols should ensure that offshore candidates are informed of the different force requirements as well as have the opportunity to practice jettisoning a high physical fidelity exit from a fully compressed crash attenuating seat.     

Random Bichromatic Matchings

Given a graph with edges colored Red and Blue, we study the problem of sampling and approximately counting the number of matchings with exactly k Red edges. We solve the problem of estimating the number of perfect matchings with exactly k Red edges for dense graphs. We study a Markov chain on the space of all matchings of a graph that favors matchings with k Red edges. We show that it is rapidly mixing using non-traditional canonical paths that can backtrack. We show that this chain can be used to sample matchings in the 2-dimensional toroidal lattice of any fixed size ℓ with k Red edges, where the horizontal edges are Red and the vertical edges are Blue. An extended abstract appeared in J.R. Correa, A. Hevia and M.A. Kiwi (eds.) Proceedings of the 7th Latin American Theoretical Informatics Symposium, LNCS 3887, pp. 190–201, Springer, 2006. N. Bhatnagar’s and D. Randall’s research was supported in part by NSF grants CCR-0515105 and DMS-0505505. V.V. Vazirani’s research was supported in part by NSF grants 0311541, 0220343 and CCR-0515186. N. Bhatnagar’s and E. Vigoda’s research was supported in part by NSF grant CCR-0455666.     

3D SSD tracking with estimated 3D planes

We present a tracking method where full camera position and orientation is tracked from intensity differences in a video sequence. The camera pose is calculated based on 3D planes, and hence does not depend on point correspondences. The plane based formulation also allows additional constraints to be naturally added, e.g., perpendicularity between walls, floor and ceiling surfaces, co-planarity of wall surfaces etc. A particular feature of our method is that the full 3D pose change is directly computed from temporal image differences without making a commitment to a particular intermediate (e.g., 2D feature) representation. We experimentally compared our method with regular 2D SSD tracking and found it more robust and stable. This is due to 3D consistency being enforced even in the low level registration of image regions. This yields better results than first computing (and hence committing to) 2D image features and then from these compute 3D pose.     

A multilingual FrameNet-based grammar and lexicon for controlled natural language

Berkeley FrameNet is a lexico-semantic resource for English based on the theory of frame semantics. It has been exploited in a range of natural language processing applications and has inspired the development of framenets for many languages. We present a methodological approach to the extraction and generation of a computational multilingual FrameNet-based grammar and lexicon. The approach leverages FrameNet-annotated corpora to automatically extract a set of cross-lingual semantico-syntactic valence patterns. Based on data from Berkeley FrameNet and Swedish FrameNet, the proposed approach has been implemented in Grammatical Framework (GF), a categorial grammar formalism specialized for multilingual grammars. The implementation of the grammar and lexicon is supported by the design of FrameNet, providing a frame semantic abstraction layer, an interlingual semantic application programming interface (API), over the interlingual syntactic API already provided by GF Resource Grammar Library. The evaluation of the acquired grammar and lexicon shows the feasibility of the approach. Additionally, we illustrate how the FrameNet-based grammar and lexicon are exploited in two distinct multilingual controlled natural language applications. The produced resources are available under an open source license.