生物制品中支原体污染的快速检测

用不ＰＣＲ法及ＤＮＡ荧光当构技术，检测了８１份不同来泊和批号的疫苗及一些实验用细胞株中的支原体，并对检测帮了比较。ＰＣＲ阳性检出率为３８．３％，Ｈｏｅｃｈｓｔ阳性检出率为１９％。这些结果表明ＰＣＲ法较Ｈｏｅｃｈｓｔ染色法更敏感，同时亦提示必须重视检测与控制国内生物制品的支原体污染问题。     

Optimisation of the medium composition for production of protease and soybean peptides by Bacillus subtilis SHZ using response surface methodology

Responses surface methodology was employed to enhance the production of protease and soybean peptides by Bacillus subtilis SHZ. For screening of medium composition significantly influencing protease and soybean peptides yield, the two-level Plackett–Burman design was used. Among thirteen variables tested; KH₂PO₄, glucose and defatted soybean flour (DSF) were selected based on their high significant effect on both protease activity and soybean peptides yield. Then, a three-level Box–Behnken design was employed to optimise the medium composition for the production of the protease and soybean peptides in submerged fermentation. Mathematical models were then developed to show the effect of each medium composition and their interactions on the production of protease and soybean peptides. The model estimated that, the maximal protease activity (320 ± 1 U mL⁻¹) could be obtained when the concentrations of glucose, KH₂PO₄, DSF were set at 8–9 g L⁻¹, 2–3 g L⁻¹, 55–65 g L⁻¹, respectively; while a maximal yield of soybean peptides (8.5 ± 0.1 g L⁻¹) could be achieved when the concentrations of glucose, KH₂PO₄, DSF were set at 7–9 g L⁻¹, 3–4 g L⁻¹ and 55–58 g L⁻¹, respectively. These predicted values were also verified by validation experiments.     

采用活性炭强化常规处理原水突发油类污染的研究

中试研究表明,常规处理(混凝—沉淀—过滤)可以将含油约为10mg/L的原水处理达标,并且除油率不受混凝剂投加量的影响。油污染浓度为7.2～18mg/L的原水经混凝沉淀去除的效率基本相同。20mg/L的油污染仅通过常规处理无法达标,需采用投加粉末活性炭(PAC)的强化混凝或颗粒活性炭(GAC)的强化过滤,即投加40mg/L的PAC,或在过滤阶段铺40cmGAC层的炭砂滤柱。KMnO4和Cl2的预氧化对除油效果无影响。     

Tb doped CoNbZr soft magnetic films with high anisotropy field for applications in GHz frequency region

CoNbZr films for an adjustable magnetic anisotropy field H_k by doping with rare-earth of different atomic ratio Tb element were obtained in this work. The effect of Tb addition ranged from 0 to 4 at.% on the magnetic properties of the amorphous CoNbZr films was further investigated. The results show that the magnetic anisotropy field H_k increases sharply with the addition of Tb while the coercivity H_ch along the hard axis and H_ce along the easy axis change slightly with increasing Tb content in the films. As a consequence, CoNbZr film doped with 2 at.% of Tb exhibits excellent soft magnetic properties with a saturation magnetization 4πM_s of 8.9 kG, a hard axis coercivity H_ch of 1.79 Oe, a easy axis coercivity H_ce of 1.4 Oe and a magnetic anisotropy field H_k of 87 Oe. The measured ferromagnetic resonance frequency f_FMR of this film is 2.30 GHz. The real permeability μ′ is about 100, which is maintained up to 2 GHz. In addition, there is a broad band of the imaginary permeability μ″ over a large frequency band, indicating high losses. Therefore, Tb doped CoNbZr film is an excellent candidate for high frequency applications such as electromagnetic interference suppressors.     

一类稀疏效应下捕食模型正平衡态解的稳定性

考虑了一类齐次Neumann边界条件下具稀疏效应和扩散的捕食者-食饵模型，通过建立适当的Lyapunov函数得到其唯一的正平衡态解的全局渐近稳定性．     

屈曲约束支撑装配式混凝土框架结构抗震性能试验研究

消能减震技术能有效地提高装配式混凝土结构的整体抗震性能,改善其破坏模式。然而,目前对屈曲约束支撑装配式混凝土框架结构尚缺乏试验研究。为了获悉屈曲约束支撑装配式混凝土框架的抗震性能和破坏机理,文章进行3榀屈曲约束支撑装配式混凝土框架和1榀现浇混凝土框架的低周反复荷载试验。研究了地震作用下采用整体式和暗牛腿式预制混凝土梁柱节点的屈曲约束支撑装配式混凝土框架抗震性能,观察和记录试验现象和破坏特征,对各试件的滞回曲线、刚度退化、耗能能力、骨架曲线和延性系数等进行对比分析。试验结果表明：屈曲约束支撑装配式混凝土框架具有良好的耗能能力和延性,能量耗散系数E=1.464?1.759,延性系数?=3.02~3.61,屈曲约束支撑可以有效地提高装配式混凝土框架的抗震性能,改善其失效模式;研发的支撑与梁柱连接节点的连接构造可以有效地实现屈曲约束支撑与装配式混凝土框架在地震作用下的协同受力。文中研究成果将为屈曲约束支撑在多高层装配式混凝土结构中的设计和应用提供科学依据。     

结合MCP和RM的检测质量评估方法

在分析现有测量系统检测质量评价算法的基础上,针对各检测实验室测量系统对产品质量控制获取满意检测结果的预控能力不足,提出基于结合测量能力指数(MCP)和标准样品(RM)的过程质量特性分析与控制方法,采用MCP对小批量柔性检测质量进行评估。实例表明,该方法可以实现对各检测实验室在小批量检测条件下的产品质量控制,在各检测实验室测量系统检测结果趋同性方面优于传统的其它检测质量评价算法。     

基于Ti1100合金的微量元素添加对合金抗氧化性能的影响

本文利用固溶体合金中的‘团簇加连接原子’模型解析了典型高温近α-Ti合金Ti1100的成分,其团簇成分式为[Al-(Ti13.7Zr0.3)](Al0.69Sn0.18Mo0.03Si0.12)。在此基础上,采用相似元素替代原则设计了微量元素Hf、Ta和Nb添加的系列合金成分,即 [Al-(Ti13.7Zr0.15Hf0.15)](Al0.69Sn0.18Si0.1(Mo/Ta/Nb)0.03)。对该系列合金进行950 ℃/1 h固溶+560 ℃/6 h时效处理,然后进行组织结构、硬度、抗高温氧化及电化学腐蚀性能测试。研究结果表明,Zr0.15Hf0.15合金与参比合金具有相同片层β转变组织,而在此基础上Ta和Nb的添加会使合金中产生大量等轴α组织;但组织的改变对系列合金的显微硬度影响不大,介于330-370 HV。650 ℃氧化100 h后系列合金均具有较强的抗氧化能力,氧化增重小于1.0 mg/cm2,而在800 ℃氧化100 h后,添加Hf、Ta、Nb元素的合金氧化增重明显低于Ti1100合金,氧化层厚度为25~27 μm,且氧化层致密,其中[Al-(Ti13.7Zr0.15Hf0.15)](Al0.69Sn0.18Si0.1Ta0.015Nb0.015)合金具有最优的抗高温氧化性能,800 ℃/100 h后的氧化增重仅为2.6 mg/cm2。此外,该系列合金在在3.5 %NaCl溶液中也具有较好的耐蚀性。     

添加氧化镁合成异丁叉二脲的工艺探究

探究加入分散剂(氧化镁)对合成异丁叉二脲(IBDU)的影响,得到了加入氧化镁时制备IBDU缓释肥料的适宜工艺条件:氧化镁的加入量占尿素质量的0.5%,尿素与异丁醛的物质的量比为3.5∶1,反应时间为60 min,尿素与水的质量比为1:4。在该条件下得到的产品易于转移抽滤,干燥后产品颗粒度更加均匀,缓释有效氮含量更高。     

微量油膜附水滴切削40Cr合金结构钢试验研究

简述了油膜附水滴(oils on water,OoW)切削液的冷却润滑原理。通过干切削、常规浇注乳化液和OoW切削液三种冷却润滑方式,切削40Cr合金结构钢的切削力以及工件表面粗糙度的对比试验,研究OoW切削液的冷却润滑性能。试验结果表明,OoW切削液能有效减小切削力和表面粗糙度值。