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31.
Insulin receptor substrate (IRS) proteins are key regulators of basic functions such as cellular growth and metabolism. They provide an interface between multiple receptors and a complex network of intracellular signaling molecules. Two members of this family (IRS-1 and IRS-2) have been identified previously. In this investigation, we analyzed a mouse expressed sequence tag clone that proved to be a new member of the IRS family. Sequence analysis of this clone and comparison with the sequences deposited in GenBank demonstrates this protein may be the murine homolog of rat IRS-3, recently purified and cloned from rat adipocytes. Accordingly, we have named our protein mouse IRS-3. The expressed sequence tag clone contains the complete coding sequence of 1485 bp, encoding a protein of 495 amino acids. Sequence alignment with the other members of the IRS family shows that this protein contains pleckstrin homology and phosphotyrosine-binding domains that are highly conserved. In addition, there is conservation of many tyrosine phosphorylation motifs responsible for interactions with downstream signaling molecules containing SH2 domains. The murine IRS-3 messenger RNA (2.4 kilobases in length) is expressed in many tissues, with highest levels in liver and lung. Mouse IRS-3 is highly expressed in the first part of the embryonic life, when IRS-1 messenger RNA is barely detectable. Unlike the genes encoding IRS-1 and IRS-2, the IRS-3 gene contains an intron (344 bp in length) in the region between the pleckstrin homology and the phosphotyrosine-binding domains. Fluorescent in situ hybridization localized the mouse IRS-3 gene on the telomeric region of chromosome 5G2. Cloning of the murine IRS-3 gene will make it possible to apply genetic approaches to elucidate the physiological role of this new member of the IRS family of proteins. 相似文献
32.
HG Morrison EJ Weil SI Karchner ML Sogin JJ Stegeman 《Canadian Metallurgical Quarterly》1998,121(1-3):231-240
Since we published a phylogenetic analysis of the CYP1A subfamily in 1995, several additional full-length sequences have been reported, including three members of an entirely new subfamily, CYP1B. Two avian sequences were recently published, so that CYP1A sequence data are now available from three of the five major vertebrate lineages. The two new branches that have been added to the CYP1 family tree significantly add to our understanding of P450 evolution. The inclusion of the CYP1Bs to the phylogenetic analysis allows us to root inferred trees. Addition of the avian CYP1As indicates that the CYP1A1/CYP1A2 duplication present in the mammalian lineage may have occurred after the divergence of birds and mammals. The number of fish species from which full-length coding regions of CYP1A genes have been sequenced has increased from four (trout, plaice, toadfish, and scup) to nine. These include CYP1A sequences from tomcod, butterflyfish, sea bream, sea bass, and the full-length sequence of CYP1A from the killifish Fundulus heteroclitus that is reported here. Phylogenetic analyses incorporating the new fish CYP1A sequences support our original conclusion that the fish CYP1As are monophyletic and indicate that the genes are evolving at very different rates in different species. 相似文献
33.
双乙醛草酰二肼直接快速光度法测定食品中痕量铜 总被引:1,自引:0,他引:1
研究了双乙醛草酸二肼与铜的显色反应,建立了分光光度法直接测定铜的新方法。试验结果表明,在pH8.0-10.0范围内,Cu2 与双乙醛草酸二肼形成稳定的紫红色配合物。该配合物在540nm处有一最大吸收峰,其表明摩尔吸光系数为2.4×104,铜量在0-12mg/50ml范围内符合比尔定律。共存离子干扰,误差仅 2.00%。用于食品中痕量钢测定.结果准确、可靠。 相似文献
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当线路末端故障时,行波在线路末端的反射情况比较复杂,反向电流行波的极性有可能与正向电流行波的极性相反,而此时测距结果又与线路无故障时相同。因此,会造成行波测距式与行波极性比较式合闸保护的不正确动作。根据行波的折、反射理论,分析出当线路无故障时正向电流行波仅在线路末端发生反射,且电流行波的反射系数为-1,而当线路存在故障时,正向电流行波将在故障点发生反射,无论故障是否在线路末端,电流行波的反射系数都不会是-1。根据这一特征,提出了一种新的行波合闸保护方法。从原理上保证了在各种情况下保护都可以正确动作,大量的电磁暂态仿真结果也证明了这一点。 相似文献
36.
Bastnasiteisamainsourceofrareearthproducts inwhichCeO2/REOisabout50%.Atpresent,a cidity leachingcombinedwithalkali conversion method[1]iscommonlyusedinthebastnasitetreat ment.Thismethodisunfriendlytotheenvironment becausetheradioactiveelementofthoriuman… 相似文献
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Yiping Wang Na Chen Binfeng Yun Yiping Cui 《Photonics Technology Letters, IEEE》2007,19(16):1242-1244
A measurement method has been developed to determine the full-field deformation of a simply supported plane plate under transverse load. The method utilizes strain information provided by a set of four fiber Bragg grating sensors mounted on the plate in a way that all sensors measure strains along one certain direction. The sensors were interrogated using a wavelength swept fiber laser. Utilizing the strain information and the first four terms of Navier solution of simply supported rectangular plate, the necessary parameters for determining the deflection could be computed. By substituting the values of x- and -y- coordinates of each position on the rectangle plate, the full-field deformation information can be obtained. Single-point loading tests were experimentally performed to verify the accuracy of the method. 相似文献
40.
La2(MoO4)3:Eu均-花瓣状微/纳米结构的构筑及其荧光性能 总被引:1,自引:0,他引:1
在表面活性剂十二烷基硫酸钠(SDS)的作用下,采用温和的水热方法,成功制备了Eu掺杂的花瓣状L丑2(MoO4)3:Eu纳米微结构。这些形貌新颖的微米绒球的直径约3μm,由厚度30nm左右的纳米片次级结构单元自组装构筑而成,分散性良好,形貌规整、大小均一。通过XRD、SEM、TEM测试技术,研究了形貌的形成机理。由于具有良好的结晶度,这些花瓣状La2(MoO4)3:Eu纳米微结构显示出良好的发光性能。 相似文献