棉粕蛋白酶解物对异育银鲫(Carassius auratus gibelio)消化、生长和胰蛋白酶mRNA表达量的影响

以棉粕蛋白为酶解底物,用枯草杆菌蛋白酶对其进行酶解,以酶解产物1.5%和3.0%两个梯度等量替代鱼饲料配方中棉粕,在室内流水养殖系统中喂养异育银鲫鱼种[体重为(30±2)g]65天。测定鱼的生长、营养物质表观消化率、消化蛋白酶活性及肝胰脏中胰蛋白酶mRNA表达水平等指标。结果表明,添加1.5%和3.0%棉粕酶解产物的鱼在饲养35天后的特定增长率(SGR)分别比对照组高32.5%和56.7%,且差异显著(P<0.05);在饲养65天后,两组特定增长率分别比对照组高8.0%和21.0%,且差异极显著(P<0.01),肝胰脏中胰蛋白酶mRNA表达水平也随棉粕酶解产物添加梯度提高而相应提高,表明鱼的生长与消化蛋白酶mRNA表达水平相关。同时棉粕蛋白酶解物对肠道蛋白酶的活性和营养物质表观消化率都有促进作用,而棉粕蛋白酶解物对鱼肌肉成分并没有改变,这也表明棉粕蛋白酶解物在促进鱼生长、内源酶活性同时并未降低鱼的品质。     

蒽胁迫对2种海洋微藻的毒性效应

采用水生毒理学方法以及通过分析蒽对 2种海洋微藻的几种生理生化指标的影响 ,初步研究了蒽胁迫对 2种海洋微藻的毒性效应。结果表明 :随着蒽浓度的不断增大 ,1 2种海洋微藻的相对增长率逐渐降低 ,叶绿素 a和类胡萝卜素含量稍有下降 ,蒽对小新月菱形藻生长的 72 h· EC50为 2 51μg· L-1 ,蒽对亚心形扁藻生长的 72 h· EC50 为 387μg· L-1 ;2还原型谷胱甘肽 (GSH)含量逐渐降低 ;3加入外源性抗氧化剂 (GSH和甘露醇 )可缓解蒽胁迫所造成的 2种海洋微藻的细胞密度的降低 ,即可缓解蒽胁迫对微藻的毒害作用     

东海大规模赤潮危害影响的实验研究——对黑褐新糠虾和卤虫的毒性效应

The effects of Prorocentrum donghaiense,Karenia mikimotoi,Alexandrium catenella,the causative species of Harmful Algal Blooms in the East China Sea,on the survival and growth of Neomysis awatschensis and Artemia salina were investigated.And the toxicity of eleven strains of Alexandrium----A.lusitanicum(AL-1)、A.minutum(AM-1)、A.spp.(A-K)、A.tamarense(ATHK,ATCI02,ATCI03,AT5-1,AT5-3,AT-6)、A.affine(AS-1,AC-1) to the two above organisms were compared. The results showed that P.donghaiense and K.mikimotoi had no adverse effect on the survival and growth of N.awatschensis and A.salina at 96h.However,the survival rate of N.awatschensis and A.salina declined linearly with the increasing density of A.catenella,and the 96h LC₅₀ for N.awatschensis and A.salina was 4500 and 460 cells/mL,respectively, Five strains of Alexandrium----A.spp,A.lusitanicum (AL-1),A.tamarense(AT5-3,AT-6) and A.affine (AS-1)at the density of 4000cells/mL and 96h had no adverse impacts on N.awatschensis. But other six strains of Alexandrium a.Tamarense(AT5-1,ATHK,ATCI02,ATCI03),A.affine(AC-1) and A.minutum(AM-1)decreased the survival fate of N.awatschensis significantly. Two strains of Alexandrium----A.lusitanicum(AL-1) and A.tamarense(AT-6),at the density of 500cells/mL. had no adverse effects on A.salina in 96h;while the other Alexandrium strains all had adverse effeets on the survival of a.salina, A.salina could increase its length with the increasing density of P.donghaiense,K.mikimotoi and A.luxitanicum. The results indicate that P.donghaiense and K.mikimotoi have nio significant effect on the survival of crustaeean zoop lankton in a short period. But several Alexandrium specised/strains could influence the survival of crustacean zooplankton likely by non-PSP substances. A.salina is shown to be more sensitive than N.awatschensis as test organisms to harmful species.     

秋季东、黄海异养细菌(Heterotrophic Bacteria)的分布特点

2000年10-11月,乘"北斗号"考察船进行秋季"东、黄海生态系统动力学与生物资源可持续利用"大面调查,研究秋季东、黄海异养细菌的分布.结果表明,异养细菌在黄海和东海的丰度分别在(2.37-13.33)×108 cell/L和(3.05-13.62)×108 cell/L之间.细菌丰度最高值出现在长江口附近,且断面E和断面F各站位的细菌丰度明显高于其他断面.异养细菌丰度大小以长江口为中心向外海依次递减.东、黄海水体异养细菌生物量分别在244.45-1812.90mgC/m2和100.60-940.87mgC/m2之间.东、黄海异养细菌丰度无显著差异,但是东海海域水体异养细菌生物量高于黄海海域.异养细菌空间分布与浮游植物叶绿素在东海有一定的相关性,黄海异养细菌与硝酸盐浓度的相关性极显著.     

EEA——一种新的、重要的海洋生态学参数及其应用

细菌胞外酶作为海洋环境中有机物与细菌相互作用的中介，其活性高低反映了细菌对相关有机组分的改造程度及该有机组分对细菌生长繁殖的营养贡献。胞外酶活性（extracellular enzyme activity,EEA)研究对于深入了解海洋生态系有着重要意义。本文概述了胞外酶活性特性、影响因素及调控机制；台湾海峡胞外酶活性研究原理，现场测定方法及影响因素，分析了台湾海峡β-葡萄糖苷酶与细菌产量、生物量的关系。     

南海北部及巴士海峡附近的水团分析

为解释黑潮水进入南海的方式 ,通过对 2 0 0 2年 5月 2 9日～ 6月 6日在南海及巴士海峡附近太平洋海域观测所得的资料进行水团分析 ,以四边形水团定量分析方法得到各水团在海区内的分布状况 ,同时分析了温度、盐度、密度和溶解氧的分布 ,并对在相同深度层次上的南海水和黑潮水性质进行了比较。观测海域的水团分为表层水团 (SW ) ,次表层水团 (SSW ) ,中层水团 (IW )和深层水团 (DW ) ,分别处于 0～ 5 0m ,5 0～ 3 0 0m ,40 0～ 10 0 0m ,10 0 0m以深。黑潮水进入南海 ,但是势力较弱 ,未能越过 119.5°E深入南海。     

不同氮源对微小亚历山大藻生长和毒素产生的影响

通过尿素、氯化铵、酵母浸出粉和硝酸钠等氮源对微小亚历山大藻(Alexandrium minutum)生长及毒素产生的影响研究,分析了微小亚历山大藻对不同氮源利用状况的差异.结果表明,在氮饥饿条件下,加入硝酸钠和酵母浸出粉能显著促进微小亚历山大藻的生长;高浓度的氯化铵在加入后对微小亚历山大藻有一定的毒性效应,表现为生长停滞,但毒性效应在5 d后消失,并得到与添加硝酸钠及酵母浸出粉相似的增长速率0.21 d-1;添加尿素对微小亚历山大藻的生长没有显著促进作用.在四种氮源中,尿素对微小亚历山大藻毒素产生的刺激作用也最弱,在稳定期每个细胞藻细胞毒素含量维持在6.00~8.00 fmol;添加硝酸钠、氯化铵和酵母浸出粉的藻细胞在稳定期毒素含量分别达到11.85,12.86和14.64 fmol.硝酸钠和氯化铵刺激藻毒素产生的效果比酵母浸出粉更为直接.四种含氮营养盐对微小亚历山大藻毒素组成的影响都很小.     

基于MODIS影像的鄱阳湖湖面积与水位关系研究

利用统计分析的方法,根据2001年获取的13景鄱阳湖区无云MODIS影像中的9景提取的水体面积,并结合同步观测的水文数据分别采用线性、对数和指数3种模型模拟湖面积-水位之间关系。结果显示对数模型相关性最好（R²=0.918）,其次为线性和指数模型。利用另外4景MODIS影像对模型进行检验表明,该模型精度较高,模拟的最大误差为3.36%。本研究显示,可根据鄱阳湖水位观测值,利用该模型预测鄱阳湖洪涝期洪水淹没面积,以弥补云天状况下光学遥感难以监测到洪水淹没范围的不足。本研究为利用遥感影像实时监控鄱阳湖水情空间动态变化提供了可行的方法,对湖泊、水库的泛洪监测、调洪功能分析具有重要意义。     

Jurassic Tectonics of North China: A Synthetic View

This paper gives a synthetic view on the Jurassic tectonics of North China, with an attempt to propose a framework for the stepwise tectonic evolution history. Jurassic sedimentation, deformation and magmatism in North China have been divided into three stages. The earliest Jurassic is marked by a period of magmatism quiescence （in 205-190 Ma） and regional uplift, which are considered to be the continuation of the “Indosinian movement” characterized by continent-continent collision between the North and South China blocks. The Early to Middle Jurassic （in 190-170 Ma） was predominated by weak lithospheric extension expressed by mantle-derived plutonism and volcanism along the Yanshan belt and alongside the Tan-Lu fault zone, normal faulting and graben formation along the Yinshan- Yanshan tectonic belt, depression and resuming of coal-bearing sedimentation in vast regions of the North China block （NCB）. The Middle to Late Jurassic stage started at 165y.5 Ma and ended up before 136 Ma; it was dominated by intensive intraplate deformation resulting from multi-directional compressions. Two major deformation events have been identified. One is marked by stratigraphic unconformity beneath the thick Upper Jurassic molasic series in the foreland zones of the western Ordos thrust-fold belt and along the Yinshan-Yanshan belt; it was predated 160 Ma. The other one is indicated by stratigraphic unconformity at the base of the Lower Cretaceous and predated 135 Ma. During this last stage, two latitudinal tectonic belts, the Yinshan-Yanshan belt in the north and the Qinling-Dabie belt in the south, and the western margin of the Ordos basin were all activated by thrusting; the NCB itself was deformed by the NE to NNE-trending structural system involving thrusting, associated folding and sinistral strike-slip faulting, which were spatially partitioned. Foliated S-type granitic plutons aged 160-150 Ma were massively emplaced in the Jiao-Liao massif east of the Tan-Lu fault zone and indicate important crustal thicken     

福建行洛坑大型钨矿的地质特征、成矿时代及其找矿意义

行洛坑钨矿是目前福建最大也是武夷山成矿带最大的钨矿,已知储量主要分布于岩体内部,宜归属于斑岩型钨矿范畴。本文通过Re-Os法和Rb-Sr等时线法测定花岗岩体中辉钼矿的Re-Os等时线年龄为156.3±4.8Ma,石英脉中流体包裹体的Rb-Sr等时线年龄为147.5±2.9Ma,表明成矿作用与岩浆岩的形成基本同步,并延续了大约10Ma。因此,行洛坑钨矿虽然属于斑岩型钨钼矿但岩体以外的空间也可能找到石英脉钨矿,而整个武夷山成矿带中生代持续而复杂的成矿历史预示了其良好的找矿前景。