Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

Overview of In - Situ Biodegradation and Enhancement

Microbial degradation technologies have been developed to restore ground water quality in aquifers polluted by organic contaminants effectively in recent years. However, in course of the degradation, the formation of biofilms in ground water remediation technology can be detrimental to the effectiveness of a ground water remediation project. Several alternatives are available to a remedial design engineer, such as Permeable Reactive Barriers (PRBs) and in -situ bioremediation, Hydrogen Releasing Compounds (HRCs) barrier, Oxygen Releasing Compounds (ORCs) barrier etc. which are efficient and cost- effective technologies. Excessive biomass formation renders a barrier ineffective in degrading the contaminants, Efforts are made to develop kinetics models which accurately determine bio - fouling and bio - filn formation and to control excessive biomass formation.     

Variability of Soft X—ray Spectral Shape in Blazars Observed by ROSAT

1 INTRODUCTION Blazars, including BL Lac objects, highly polarized and optically violently variable quasars,and flat-spectrum radio quasars (FSRQs), are characterized by highly variable non-thermalemission which dominates their characteristics from radio to y-ray bands. The mechanismbelieved to be responsible for their broadband emission is synchrotron radiation followed by in-verse Compton (IC) scattering at higher energies (e.g. Blandford & Konigl 1979). Relativisticbeaming of a jet…     

大同—阳高Ms6.1地震前兆综合加权信息熵的变化

利用“地震预报计算机专家系统”的思想对大同 -阳高 Ms6 .1地震前每一前兆异常事件进行综合评估 ,以每一异常的最可能发震时间来计算发震概率 ,利用地震前兆综合加权信息熵研究了系统熵值与地震的关系。对华北地区的地震前兆综合加权信息熵研究表明 ,在大同 -阳高 Ms6 .1地震前 ,信息熵出现了明显的减熵有序变化     

CHANGE OF NUTRIENT IMPORT AND EXPORT IN PROCESS OF RAINFALL IN AILAO MOUNTAIN OF YUNNAN PROVINCE

ＣＨＡＮＧＥ　ＯＦ　ＮＵＴＲＩＥＮＴ　ＩＭＰＯＲＴ　ＡＮＤ　ＥＸＰＯＲＴ　ＩＮ　ＰＲＯＣＥＳＳ　ＯＦ　ＲＡＩＮＦＡＬＬ　ＩＮ　ＡＩＬＡＯ　ＭＯＵＮＴＡＩＮ　ＯＦ　ＹＵＮＮＡＮ　ＰＲＯＶＩＮＣＥＧａｎＪｉａｎｍｉｎ（甘健民）；ＸｕｅＪｉｎｇｙｉ（薛敬意...     

长江上游洪涝暴雨环流形势中期演变趋势分析

本文通过对1980年以来长江上游10次洪涝过程的500hpa环流形势分析,并用车比雪夫多项式对500hpa高度场进行展开,研究了各类洪涝过程的中期演变特征,发现代表不同洪涝过程环流形势变化的不同的车氏系数值都有明显的特征变化,结合欧洲中心中期数值预告96小时500hpa高度场的车氏系数分析,可为洪涝暴雨的中期预报提供一种新的思路。     

P‐wave transmission across fractures with nonlinear deformational behaviour

Stress wave attenuation across fractured rock masses is a great concern of underground structure safety. When the wave amplitude is large, fractures experience nonlinear deformation during the wave propagation. This paper presents a study on normal transmission of P‐wave across parallel fractures with nonlinear deformational behaviour (static Barton–Bandis model). The results show that the magnitude of transmission coefficient is a function of incident wave amplitude, nondimensional fracture spacing and number of fractures. Two important indices of nondimensional fracture spacing are identified, and they divide the area of nondimensional fracture spacing into three parts (individual fracture area, transition area and small spacing area). In the different areas, the magnitude of transmission coefficient has different trends with nondimensional fracture spacing and number of fractures. In addition, the study reveals that under some circumstances, the magnitude of transmission coefficient increases with increasing number of fractures, and is larger than 1. Copyright © 2006 John Wiley & Sons, Ltd.     

可持续发展进程中的政府能力建设

可持续发展作为现代化建设的两大战略之一 ,对我国政府的能力提出了挑战。可持续发展战略的实施 ,要求加强政府的作用 ,将政府建设成为强有力而又廉价的政府。为此 ,我国政府应从加强政府的财政汲取能力建设、强化中央政府协调地区发展的能力 ,加强政府调控能力建设、强化中央政府协调当前利益和长远利益、经济利益与生态效益的能力 ,并从根本上转变政府职能、精兵简政、建设廉价高效的政府等方面着手来提高我国政府推进和实施可持续发展战略的能力     

星风吸积引起的钡星重元素超丰

首先指出目前星风吸积模型中理论上的不自洽，考虑到δｒ≠０，重新推导轨道参量变化方程，消去了理论上的不自洽．提出一个新模型：首次将星风吸积同内禀ＡＧＢ星核合成模型结合起来计算钡（Ｂａ）星的重元素超丰，并将计算结果与观测值进行了比较．各参量按标准情况取值时，计算结果不太理想．取Ｂｏｎｄｉｈｏｙｌｅ吸积率的０．５倍或取较大的星风速率时，对于较长轨道周期（Ｐ＞１０００天）的Ｂａ星，计算结果与观测值基本符合；而较短轨道周期（Ｐ＜６００天）的Ｂａ星，其重元素超丰机制可能是盘吸积     

一种基于自证明公钥的可公开验证的认证加密方案

针对现有的基于证书的认证加密方案通信量和计算量大的缺点,提出了基于自证明公钥的可公开验证的认证加密方案。详细阐述了方案的系统初始化、签名生成、消息恢复验证以及公开验证4个阶段,方案的安全性是基于求解离散对数的困难性(DLP)和强单向hash函数(OWFH)的不可逆。该方案在没有签名者的协作下,任何验证者都可验证签名者的诚实性,在验证签名的同时可认证签名者的公钥,因而减少了存储空间、通信量和计算量。