Spatial and temporal variation of biomarkers in mussels (Mytilus galloprovincialis) from the Lagoon of Venice, Italy

A multiple biomarker approach was adopted in a seasonal study carried out in the Lagoon of Venice, with the double aim of evaluating the natural and anthropic stresses influencing the biological responses of mussel (Mytilus galloprovincialis) and of assessing the effects due to spatial rather than temporal variations.Biochemical (aldehyde dehydrogenase and catalase activities), cellular (neutral red retention time) and physiological (survival in air and condition index) biomarkers were determined in mussels collected in four differently impacted lagoon areas.Multivariate analysis showed that samples were distributed mainly according to temperature and four seasonal groups were identified. The combination of spatial and temporal information enabled us to distinguish physiological variations due to natural causes from those due to anthropic stress, and to identify the sampling period when several biomarkers are less influenced by both natural and endogenous factors.     

Factors affecting habitat occupancy of fish assemblage in the Gulf of Trieste (Northern Adriatic Sea)

Species composition, richness and abundance of the fish assemblage were studied over six different inshore macrohabitats in the southern part of the Gulf of Trieste (Northern Adriatic Sea) using the visual counts technique. Fifteen environmental variables were taken into consideration in order to determine microhabitat preferences of fish species, using canonical correspondence analysis and electivity indices. The results suggest that the structure of the fish assemblage in shallow habitats is affected by a large number of interplaying factors. Depth, type of bottom and vegetation cover – incorporating both abiotic and biotic variables – are some of the factors responsible for coastal fish distribution. Microhabitat preferences for 29 fish species are presented in the depth range from 0.5 to 3 m.     

Spatial and temporal variation of biomarkers in mussels (Mytilus galloprovincialis) from the Lagoon of Venice, Italy

A multiple biomarker approach was adopted in a seasonal study carried out in the Lagoon of Venice, with the double aim of evaluating the natural and anthropic stresses influencing the biological responses of mussel (Mytilus galloprovincialis) and of assessing the effects due to spatial rather than temporal variations. Biochemical (aldehyde dehydrogenase and catalase activities), cellular (neutral red retention time) and physiological (survival in air and condition index) biomarkers were determined in mussels collected in four differently impacted lagoon areas. Multivariate analysis showed that samples were distributed mainly according to temperature and four seasonal groups were identified. The combination of spatial and temporal information enabled us to distinguish physiological variations due to natural causes from those due to anthropic stress, and to identify the sampling period when several biomarkers are less influenced by both natural and endogenous factors.     

Gravity for Lithosphere Architecture Determination and Analysis: the Central Eastern Mediterranean case study

The Central-Eastern Mediterranean region is known to be a complex area due to the interaction of four tectonic plates, namely Arabia, Africa, Anatolia and Eurasia, and by the presence of an ancient oceanic crust in the Herodotus and Ionian Basins. The analysis of the available literature highlights that the distribution of the freely available geophysical data (i.e. seismic, gravity and magnetic observations) is quite disparate. In this framework, high-resolution global gravity field models, such as XGM2019e, mainly based (offshore) on satellite data, can be profitably used as a uniform dataset to study, in a coherent way, large regions. In the current work, we exploit the XGM2019e model, together with a set of a priori information, derived mainly from geophysical data retrieved from the literature, to study the structure of the crust in the Central-Eastern Mediterranean area. The study is organized in three different phases: in the first one, we enhanced the map of geological crustal provinces by means of an automatic Bayesian classification algorithm applied to the second radial derivatives of the gravitational potential. In the second phase, using as observation a grid of gravity anomalies, we applied a full three-dimensional inversion procedure (always based on a Bayesian paradigm) to estimate the mass density variations and the geometries of the main geological units in the whole study area. Finally, in the third phase, we performed a refined three-dimensional local inversion on the Cyprus area to improve the modelling of the shallowest layers. The main results of this study, carried out in the framework of the European Space Agency GIADA project, are freely available, upon request, at https://www.g-red.eu/geophysics/ .     

Ocean urea fertilization for carbon credits poses high ecological risks

The proposed plan for enrichment of the Sulu Sea, Philippines, a region of rich marine biodiversity, with thousands of tonnes of urea in order to stimulate algal blooms and sequester carbon is flawed for multiple reasons. Urea is preferentially used as a nitrogen source by some cyanobacteria and dinoflagellates, many of which are neutrally or positively buoyant. Biological pumps to the deep sea are classically leaky, and the inefficient burial of new biomass makes the estimation of a net loss of carbon from the atmosphere questionable at best. The potential for growth of toxic dinoflagellates is also high, as many grow well on urea and some even increase their toxicity when grown on urea. Many toxic dinoflagellates form cysts which can settle to the sediment and germinate in subsequent years, forming new blooms even without further fertilization. If large-scale blooms do occur, it is likely that they will contribute to hypoxia in the bottom waters upon decomposition. Lastly, urea production requires fossil fuel usage, further limiting the potential for net carbon sequestration. The environmental and economic impacts are potentially great and need to be rigorously assessed.     

Freshwater chlorophycean algae in recent marine sediments of the Beaufort, Laptev and Kara Seas (Arctic Ocean) as indicators of river runoff

Freshwater chlorophycean algae are characteristic organic-walled microfossils in recent coastal and shelf sediments from the Beaufort, Laptev and Kara seas (Arctic Ocean). The persistent occurrence of the chlorophycean algae Pediastrum spp. and Botryococcus cf. braunii in marine palynomorph assemblages is related to the discharge of freshwater and suspended matter from the large Siberian and North American rivers into the Arctic shelf seas. The distribution patterns of these algae in the marine environments reflect the predominant deposition of riverine sediments and organic matter along the salinity gradient from the outer estuaries and prodeltas to the shelf break. Sedimentary processes overprint the primary distribution of these algae. Resuspension of sediments by waves and bottom currents may transport sediments in the bottom nepheloid layer along the submarine channels to the shelf break. Bottom sediments and microfossils may be incorporated into sea ice during freeze-up in autumn and winter leading to an export from the shelves into the deep sea. The presence of these freshwater algae in sea-ice and bottom sediments in the central Arctic Ocean confirm that transport in sea ice is an important process which leads to a redistribution of shallow water microfossils.     

Effects of levee cover strength on flood mapping in the case of levee breach due to overtopping

The reliability of a levee system is a crucial factor in flood risk management. In this study we present a probabilistic methodology to assess the effects of levee cover strength on levee failure probability, triggering time, flood propagation and consequent impacts on population and assets. A method for determining fragility curves is used in combination with the results of a one-dimensional hydrodynamic model to estimate the conditional probability of levee failure in each river section. Then, a levee breach model is applied to calculate the possible flood hydrographs, and for each breach scenario a two-dimensional hydrodynamic model is used to estimate flood hazard (flood extent and timing, maximum water depths) and flood impacts (economic damage and affected population) in the areas at risk along the river reach. We show an application for levee overtopping and different flood scenarios for a 98 km reach of the lower Po River in Italy. The results show how different design solutions for the levee cover can influence the probability of levee failure and the consequent flood scenarios. In particular, good grass cover strength can significantly delay levee failure and reduce maximum flood depths in the flood-prone areas, thus helping the implementation of flood risk management actions.

EDITOR D. Koutsoyiannis

ASSOCIATE EDITOR A. Viglione     

Long-term perspective on the relationship between phytoplankton and nutrient concentrations in a southeastern Australian estuary

Sixteen years (1997–2013) of physicochemical, nutrient and phytoplankton biomass (Chlorophyll-a (Chl-a)) data and a decade (2003-2013) of phytoplankton composition and abundance data were analyzed to assess how the algal community in a temperate southeastern Australian estuary has responded to decreased chronic point source nitrogen loading following effluent treatment upgrade works in 2003. Nitrogen concentrations were significantly lower (P < 0.05) following enhanced effluent treatment and Chl-a levels decreased (P < 0.05) during the warmer months. Temperature and nutrient concentrations significantly influenced temporal changes of Chl-a (explaining 55% of variability), while salinity, temperature, pH and nutrient concentrations influenced phytoplankton abundance and composition (25% explained). Harmful Algal Bloom (HAB) dynamics differed between sites likely influenced by physical attributes of the estuary. This study demonstrates that enhanced effluent treatment can significantly decrease chronic point source nitrogen loading and that Chl-a concentrations can be lowered during the warmer months when the risk of blooms and HABs is greatest.     

A standardized method for sampling and extraction methods for quantifying microplastics in beach sand

Microplastics are ubiquitous in the environment, are frequently ingested by organisms, and may potentially cause harm. A range of studies have found significant levels of microplastics in beach sand. However, there is a considerable amount of methodological variability among these studies. Methodological variation currently limits comparisons as there is no standard procedure for sampling or extraction of microplastics. We identify key sampling and extraction procedures across the literature through a detailed review. We find that sampling depth, sampling location, number of repeat extractions, and settling times are the critical parameters of variation. Next, using a case-study we determine whether and to what extent these differences impact study outcomes. By investigating the common practices identified in the literature with the case-study, we provide a standard operating procedure for sampling and extracting microplastics from beach sand.