Titanium Isotope Analysis of Igneous Reference Materials using a Double-spike MC-ICP-MS Method

Ti separation was achieved by ion-exchange chromatography using Bio-Rad AG 1-X8 anion-exchange and DGA resins. For high-Fe/Ti and high-Mg/Ti igneous samples, a three-column procedure was required, whereas a two-column procedure was used for low-Fe/Ti and low-Mg/Ti igneous samples. The Ti isotopes were analysed by MC-ICP-MS, and instrumental mass bias was corrected using a 47Ti-49Ti double-spike technique. The 47Ti-49Ti double-spike and SRM 3162a were calibrated using SRM 979-Cr, certificated value 53Cr/52Crtrue = 0.11339. Isobaric interference was evaluated by analysing Alfa-Ti doped with Na, Mg, Ca, and Mo, and results indicate that high concentrations of Na and Mg have no significant effect on Ti isotope analyses; however, Ca and Mo interferences lead to erroneous δ49/47Ti values when Ca/Ti and Mo/Ti ratios exceed 0.01 and 0.1, respectively. Titanium isotopic compositions were determined for 12 igneous reference materials, BCR-2, BHVO-2, GBW07105, AGV-1, AGV-2, W-2, GBW07123, GBW07126, GBW07127, GBW07101, JP-1, and DTS-2b. Samples yield δ49/47Ti (‰) of ?0.035 ± 0.022, ?0.038 ± 0.031, 0.031 ± 0.022, 0.059 ± 0.038, 0.044 ± 0.037, 0.000 ± 0.015, 0.154 ± 0.044, ?0.044 ± 0.018, 0.010 ± 0.022, 0.064 ± 0.043, 0.169 ± 0.034, and ?0.047 ± 0.025 (relative to OL-Ti, ±2SD), respectively; of which isotopic compositions of DTS-2b, JP-1, GBW07101, GBW07105, GBW07123, GBW07126, and GBW07127 are reported for the first time. Standard Alfa-Ti was analysed repeatedly over a ten-month period, indicating a reproducibility of ±0.047 (2SD) for δ49/47Ti, similar to the precisions obtained for geochemical reference materials.     

大气中痕量有机胺测量方法的研究进展

作为大气中最重要的含氮有机化合物,有机胺易与硫酸、硝酸气体生成胺盐,促进新颗粒的形成,同时也可与臭氧、OH自由基、NO₃自由基发生氧化反应,是二次气溶胶的前体物.综述了国内外关于大气有机胺检测技术的研究进展,对常规的离线检测技术和在线质谱技术进行了介绍和对比,重点介绍了质谱法工作原理以及测定大气有机胺的优越性,并对其未来发展进行了展望.     

Associations of decimetric type Ⅲ bursts with coronal mass ejections and Hα flares

We present a statistical study of decimetric type Ⅲ radio bursts,coronal mass ejections(CMEs),and Hα flares observed in the period from July 2000 to March2005.In total,we investigated 395 decimetric type Ⅲ radio burst events,21% of which showed apparent correlation to CMEs that were associated with Hα flares.We noticed that the Hα flares which were strongly associated with CMEs were gradual events,and82% of them took place before CMEs appeared in the field of view of LASCO C2;that most of the CME-associated radio bursts started in the frequency range around750 MHz with a frequency drifting rate of several hundred MHz s-1,of which both positive and negative ones were recognized; and that the correlation of type Ⅲ radio bursts to CMEs without associated flares is fairly vague,less than 9%.     

马氏珠母贝(Pinctada fucata)组织蛋白酶L基因的克隆与表达分析

根据已构建的溶藻弧菌(Vibro alginolyticus)诱导的马氏珠母贝(Pinctada fucata)血淋巴cDNA差减文库得到的ESTs序列, 应用cDNA末端快速扩增(RACE)技术成功克隆了其组织蛋白酶L基因(PFCatL), 并对其进行了生物信息学分析; 应用实时荧光定量PCR (Real-time PCR)技术, 研究了PFCatL基因在溶藻弧菌刺激前后马氏珠母贝足、外套膜、鳃、闭壳肌等8个组织中的表达变化。结果表明, PFCatL基因cDNA全长2004bp, 其中5′非编码区(5′-UTR)50bp, 3′非编码区(3′-UTR)865bp, 开放阅读框(ORF)1089bp, 编码362个氨基酸, 其分子量计算值(MW)为40.52kDa, 理论等电点(IP)为5.20; 生物信息学分析表明, PFCatL含有16个氨基酸残基组成的信号肽序列以及组织蛋白酶前体抑制功能域I29; Clustalw2多重比对发现PFCatL氨基酸序列在催化三联体Cys-His-Asn、底物结合位点以及二硫键形成相关的半胱氨酸残基位点高度保守; Real-time PCR研究发现, PFCatL在马氏珠母贝各组织中均有表达, 但各组织间的表达量存在差异, 其中以肾和闭壳肌中的表达量最高; 溶藻弧菌感染4h后, 外套膜、鳃以及血淋巴中PFCatL基因的表达较感染前显著上调。     

海水鱼类寄生虫刺激隐核虫(Cryptocaryon irritans)趋化性研究

应用本实验室建立的趋化模型对海水鱼类寄生虫刺激隐核虫(Cryptocaryon irritans)的趋化特性进行研究。结果显示, 刺激隐核虫幼虫在30min时趋化达到饱和, 在温度28℃时趋化率达到最高; 多种硬骨鱼的血清与粘液对刺激隐核虫的幼虫都有着强烈的吸引作用, 刺激隐核虫幼虫对碳水化合物、氨基酸等小分子物质没有趋化作用; 在组织匀浆液中, 刺激隐核虫幼虫对肌肉匀浆液有一定的趋化作用, 对肝、肠、胃的匀浆液都没有趋化作用; 感染后鱼类血清对刺激隐核虫的幼虫有着更加强烈的吸引作用(P<0.01); 18种中草药中的五倍子、大黄、槟榔、苦楝、虎杖、贯众等6种中药草对刺激隐核虫的幼虫有一定的驱虫作用。本文的研究结果有助于深化对刺激隐核虫致病性的认识并对“白点病”的防治有一定的参考价值。     

The improvement of the one-dimensional Mellor-Yamada and K-profile parameterization turbulence schemes with the non-breaking surface wave-induced verticalmixing

Both the level 2.5 Mellor-Yamada turbulence closure scheme(MY) and K-profile parameterization(KPP) are popularly used by the ocean modeling community.The MY and the KPP are improved through including the non-breaking surface wave-induced vertical mixing(Bv),and the improved schemes were tested by using continuous data at the Papa ocean weather station(OWS) during 1961–1965.The numerical results showed that the Bv can make the temperature simulations fit much better with the continuous data from Papa Station.The two improved schemes overcame the shortcomings of predicting too shallow upper mixed layer depth and consequently overheated sea surface temperature during summertime,which are in fact common problems for all turbulence closure models.Statistical analysis showed that the Bv effectively reduced the mean absolute error and root mean square error of the upper layer temperature and increased the correlation coefficient between simulation and the observation.Furthermore,the performance of vertical mixing induced by shear instability and the Bv is also compared.Both the temperature structure and its seasonal cycle significantly improved by including the Bv,regardless of whether shear instability was included or not,especially for the KPP mixing scheme,which suggested that Bv played a dominant role in the upper ocean where the mean current was relatively weak,such as at Papa Station.These results may provide a clue to improve ocean circulation models.     

Rainfall effect on wind waves and the turbulence beneath air-sea interface

Rainfall effects on wind waves and turbulence are investigated through the laboratory experiments in a large wind-wave tank. It is found that the wind waves are damped as a whole at low wind speeds, but are enhanced at high wind speeds. This dual effect of rain on the wind waves increases with the increase of rain rate, while the influence of rainfall-area length is not observable. At the low wind speed, the corresponding turbulence in terms of the turbulent kinetic energy （TKE） dissipation rate is significantly enhanced by rain- fall as the waves are damped severely. At the high wind speed, the augment of the TKE dissipation rate is suppressed while the wind waves are enhanced simultaneously. In the field, however, rainfall usually hin- ders the development of waves. In order to explain this contradiction of rainfall effect on waves, a possibility about energy transfer from turbulence to waves in case of the spectral peak of waves overlapping the inertial subrange of turbulence is assumed. It can be applied to interpret the damping phenomenon of gas trans- fer velocity in the laboratory experiments, and the variation of the TKE dissipation rates near sea surface compared with the law of wall.     

The vertical structure of the atmospheric boundary layer over the central Arctic Ocean

The tropopause height and the atmospheric boundarylayer （PBL） height as well as the variation of inversion layer above the floating ice surface are presented using GPS （global position system ） radiosonde sounding data and relevant data obtained by Chinas fourth arctic scientific expedition team over the central Arctic Ocean （86°-88°N, 144°-170°W） during the summer of 2010. The tropopause height is from 9.8 to 10.5 km, with a temperature range between -52.2 and -54.10C in the central Arctic Ocean. Two zones of maximum wind （over 12 m/s） are found in the wind profile, namely, low- and upper-level jets, located in the middle troposphere and the tropopause, respectively. The wind direction has a marked variation point in the two jets from the southeast to the southwest. The average PBL height determined by two methods is 341 and 453 m respectively. These two methods can both be used when the inversion layer is very low, but the results vary significantly when the inversion layer is very high. A significant logarithmic relationship exists between the PBL height and the inversion intensity, with a correlation coefficient of 0.66, indicating that the more intense the temperature inversion is, the lower the boundary layer will be. The observation results obviously differ from those of the third arctic expedition zone （800-85° N）. The PBL height and the inversion layer thickness are much lower than those at 870-88° N, but the inversion temperature is more intense, meaning a strong ice- atmosphere interaction in the sea near the North Pole. The PBL structure is related to the weather system and the sea ice concentration, which affects the observation station.     

Sedimentary Environments of Cambrian–Ordovician Source Rocks and Ultra-deep Petroleum Accumulation in the Tarim Basin

The Tarim Basin is the only petroliferous basin enriched with marine oil and gas in China. It is presently also the deepest basin for petroleum exploration and development in the world. There are two main sets of marine Source Rocks (SRs) in the Tarim Basin, namely the high over-mature Cambrian–Lower Ordovician (∈–O1) and the moderately mature Middle–Upper Ordovician (O2–3). The characteristic biomarkers of SRs and oils indicate that the main origin of the marine petroleum is a mixed source of ∈–O1 and O2–3 SRs. With increasing burial, the hydrocarbon contribution of the ∈–O1 SRs gradually increases. Accompanied by the superposition of multi-stage hydrocarbon-generation of the SRs and various secondary alteration processes, the emergence and abnormal enrichment of terpenoids, thiophene and trimethylaryl isoprenoid in deep reservoirs indicate a complex genesis of various deep oils and gases. Through the analysis of the biofacies and sedimentary environments of the ∈–O1 and O2–3 SRs, it is shown that the lower Paleozoic high-quality SRs in the Tarim Basin were mainly deposited in a passive continental margin and the gentle slope of the platform, deep-water shelf and slope facies, which has exhibited a good response to the local tectonic-sedimentary environment. The slope of the paleo-uplift is the mutual area for the development of carbonate reservoirs and the deposition of marine SRs, which would be favorable for the accumulation of petroleum. Due to the characteristics of low ground temperature, the latest rapid and deep burial does not cause massive oil-cracking in the paleo-uplift and slope area. Therefore, it is speculated that the marine reservoirs in the slope of the Tabei Uplift are likely to be a favorable area for deep petroleum exploration, while the oil-cracking gas would be a potential reserve around the west margin of the Manjiaer Depression. Hydrocarbons were generated from various unit SRs, mainly migrating along the lateral unconformities or reservoirs and the vertical faults. They eventually brought up three major types of exploration fields: middle and lower Cambrian salt-related assemblages, dolomite inner reservoirs and Middle and Lower Ordovician oil-bearing karst, which would become the most favorable target of marine ultra-deep exploration in the Tarim Basin.     

LITHOPROBE——加拿大地球探测计划

LITHOPROBE是加拿大的国家级地球科学研究计划。选择10个典型剖面,每个都不同程度地代表了加拿大典型的地质特征或具全球意义的重要构造过程。时间上其地质演化跨越了40多亿年漫长的地质年代。通过地震深反射技术为先锋的以及多种不同学科的综合研究,取得一系列成果,如证实3.0Ga前即发生与板块构造有关的作用,对古老岩石圈板块碰撞和新地壳形成过程进行了重大修正,揭示了若干大型矿集区的深部控矿构造的反射影像,使加拿大的地球科学研究走到世界的前列,本文拟通过较系统介绍LITHOPROBE,为我国深部探测技术与实验研究提供参考。