Chronic ingestion of a low dose of caffeine induces tolerance to the performance benefits of caffeine

This study examined effects of 4 weeks of caffeine supplementation on endurance performance. Eighteen low-habitual caffeine consumers (<75 mg · day^?1) were randomly assigned to ingest caffeine (1.5–3.0 mg · kg^?1day^?1; titrated) or placebo for 28 days. Groups were matched for age, body mass, V?O_2peak and W_max (P > 0.05). Before supplementation, all participants completed one V?O_2peak test, one practice trial and 2 experimental trials (acute 3 mg · kg^?1 caffeine [precaf] and placebo [testpla]). During the supplementation period a second V?O_2peak test was completed on day 21 before a final, acute 3 mg · kg^?1 caffeine trial (postcaf) on day 29. Trials consisted of 60 min cycle exercise at 60% V?O_2peak followed by a 30 min performance task. All participants produced more external work during the precaf trial than testpla, with increases in the caffeine (383.3 ± 75 kJ vs. 344.9 ± 80.3 kJ; Cohen’s d effect size [ES] = 0.49; P = 0.001) and placebo (354.5 ± 55.2 kJ vs. 333.1 ± 56.4 kJ; ES = 0.38; P = 0.004) supplementation group, respectively. This performance benefit was no longer apparent after 4 weeks of caffeine supplementation (precaf: 383.3 ± 75.0 kJ vs. postcaf: 358.0 ± 89.8 kJ; ES = 0.31; P = 0.025), but was retained in the placebo group (precaf: 354.5 ± 55.2 kJ vs. postcaf: 351.8 ± 49.4 kJ; ES = 0.05; P > 0.05). Circulating caffeine, hormonal concentrations and substrate oxidation did not differ between groups (all P > 0.05). Chronic ingestion of a low dose of caffeine develops tolerance in low-caffeine consumers. Therefore, individuals with low-habitual intakes should refrain from chronic caffeine supplementation to maximise performance benefits from acute caffeine ingestion.     

Caffeine ingestion enhances perceptual responses during intermittent exercise in female team-game players

We examined the influence of caffeine supplementation on cognitive performance and perceptual responses in female team-game players taking low-dose monophasic oral contraceptives of the same hormonal composition. Ten females (24 ± 4 years; 59.7 ± 3.5 kg body mass; 2–6 training sessions per week) took part in a randomised, double-blind, placebo-controlled crossover-design trial. A 90-min intermittent treadmill-running protocol was completed 60 min following ingestion of a capsule containing either 6 mg ? kg^?1 anhydrous caffeine or artificial sweetener (placebo). Perceptual responses (ratings of perceived exertion (RPE), feeling scale (FS), felt arousal scale (FAS)), mood (profile of mood states (POMS)) and cognitive performance (Stroop test, choice reaction time (CRT)) were completed before, during and after the exercise protocol, as well as after ~12 h post exercise. Caffeine ingestion significantly enhanced the ratings of pleasure (P = 0.008) and arousal (P = 0.002) during the exercise protocol, as well as increased vigour (POMS; P = 0.007), while there was a tendency for reduced fatigue (POMS; P = 0.068). Caffeine ingestion showed a tendency to decrease RPE (P = 0.068) and improve reaction times in the Stroop (P = 0.072) and CRT (P = 0.087) tests. Caffeine supplementation showed a positive effect on perceptual parameters by increasing vigour and a tendency to decrease fatigue during intermittent running activity in female games players taking low-dose monophasic oral contraceptive steroids (OCS).     

Repeated sprint ability in young basketball players: one vs. two changes of direction (Part 2)

The aim of this study was to compare the training effects based on repeated sprint ability (RSA) (with one change of direction) with an intensive repeated sprint ability (IRSA) (with two changes of direction) on jump performance and aerobic fitness. Eighteen male basketball players were assigned to repeated sprint ability and intensive repeated sprint ability training groups (RSAG and IRSAG). RSA, IRSA, squat jump (SJ), countermovement jump (CMJ) and Yo-Yo intermittent recovery level 1 test were assessed before and after four training weeks. The RSA and IRSA trainings consisted of three sets of six sprints (first two weeks) and eight sprints (second two weeks) with 4-min sets recovery and 20-s of sprints recovery. Four weeks of training led to an overall improvement in most of the measures of RSA, but little evidence of any differences between the two training modes. Jump performance was enhanced: CMJ of 7.5% (P < 0.0001) and 3.1% (P = 0.016) in IRSAG and RSAG respectively. While SJ improved of 5.3% (P = 0.003) for IRSAG and 3.4% (P = 0.095) for RSAG. Conversely the Yo-Yo distance increased 21% (P = 0.301) and 34% (P = 0.017) in IRSAG and RSAG respectively. Therefore, short-term repeated sprint training with one/two changes of direction promotes improvements in both RSA and IRSA respectively but the better increase on jump performance shown a few changes on sprint and endurance performances.     

Effects of the Nordic Hamstring exercise on sprint capacity in male football players: a randomized controlled trial

This assessor-blinded, randomized controlled superiority trial investigated the efficacy of the 10-week Nordic Hamstring exercise (NHE) protocol on sprint performance in football players.

Thirty-five amateur male players (age: 17–26 years) were randomized to a do-as-usual control group (CG; n = 17) or to 10-weeks of supervised strength training using the NHE in-season (IG; n = 18). A repeated-sprint test, consisting of 4 × 6 10 m sprints, with 15 s recovery period between sprints and 180 s between sets, was conducted to evaluate total sprint time as the primary outcome. Secondary outcomes were best 10 m sprint time (10mST) and sprint time during the last sprint (L10mST). Additionally, peak eccentric hamstring strength (ECC-P_HS) and eccentric hamstring strength capacity (ECC-CAP_HS) were measured during the NHE.

Ten players were lost to follow-up, thus 25 players were analyzed (CG n = 14; IG n = 11). Between-group differences in mean changes were observed in favor of the IG for sprint performance outcomes; TST (?0.649 s, p = 0.056, d = 0.38), 10mST (?0.047 s, p = 0.005, d = 0.64) and L10mST (?0.052 s, p = 0.094, d = 0.59), and for strength outcomes; ECC-P_HS (62.3 N, p = 0.006, d = 0.92), and ECC-CAP_HS (951 N, p = 0.005, d = 0.95).

In conclusion, the NHE showed small-to-medium improvements in sprint performance and large increases in peak eccentric hamstring strength and capacity.

Trial Registration Number: NCT02674919     

Acute adaptations and subsequent preservation of strength and speed measures following a Nordic hamstring curl intervention: a randomised controlled trial

This randomised controlled trial investigated changes in eccentric hamstring strength, 10m sprint speed, and change-of-direction (COD) performance immediately post Nordic hamstring curl (NHC) intervention and following a 3-week detraining period.

Fourteen male team sports athletes were randomised to a do-as-usual control group (CG; n = 7) or to a NHC intervention group (NHC; n = 7). Isokinetic dynamometry at 180°/s evaluated eccentric hamstring strength immediately post-intervention as the primary outcome measure. Secondary outcomes included 10 m sprint time and COD. Each outcome was measured, pre, immediately post-intervention and following a 3-week detraining period.

Immediately post-intervention significant group differences were observed in the NHC group for eccentric hamstring strength (31.81 Nm^?1 vs. 6.44 Nm^?1, P = 0.001), COD (?0.12 s vs. 0.20 s; P = 0.003) and sprint (- 0.06 s vs. 0.05 s; P = 0.024) performance. Performance improvements were maintained following a detraining period for COD (?0.11 s vs. 0.20 s; P = 0.014) and sprint (?0.05 s vs. 0.03 s, P = 0.031) but not eccentric hamstring strength (15.67 Nm^?1 vs. 6.44 Nm^?1, P = 0.145) These findings have important implications for training programmes designed to reduce hamstring injury incidence, whilst enhancing physical qualities critical to sport.     

The effects of combined glucose-electrolyte and sodium bicarbonate ingestion on prolonged intermittent exercise performance

Abstract

This study examined the effects of combined glucose and sodium bicarbonate ingestion prior to intermittent exercise. Ninemales (mean ± s age 25.4 ± 6.6 years, body mass 78.8 ± 12.0 kg, maximal oxygen uptake ([Vdot]O_2max) 47.0 ± 7ml · kg · min^?1) undertook 4 × 45 min intermittent cycling trials including 15 × 10 s sprints one hour after ingesting placebo (PLA), glucose (CHO), sodium bicarbonate (NaHCO₃) or a combined CHO and NaHCO₃ solution (COMB). Post ingestion blood pH (7.45 ± 0.03, 7.46 ± 0.03, 7.32 ± 0.05, 7.32 ± 0.01) and bicarbonate (30.3 ± 2.1, 30.7 ± 1.8, 24.2 ± 1.2, 24.0 ± 1.8 mmol · l^?1) were greater for NaHCO₃ and COMB when compared to PLA and CHO, remaining elevated throughout exercise (main effect for trial; P < 0.05). Blood lactate concentration was greatest throughout exercise for NaHCO₃ and COMB (main effect for trial; P < 0.05). Blood glucose concentration was greatest 15 min post-ingestion for CHO followed by COMB, NaHCO₃ and PLA (7.13 ± 0.60, 5.58 ± 0.75, 4.51 ± 0.56, 4.46 ± 0.59 mmol · l^?1, respectively; P < 0.05). Gastrointestinal distress was lower during COMB compared to NaHCO₃ at 15 min post-ingestion (P < 0.05). No differences were observed for sprint performance between trials (P = 1.00). The results of this study suggest that a combined CHO and NaHCO₃ beverage reduced gastrointestinal distress and CHO availability but did not improve performance. Although there was no effect on performance an investigation of the effects in more highly trained individuals may be warranted.     

Caffeine supplementation does not affect match activities and fatigue resistance during match play in young football players

Abstract

The study examined the effect of caffeine supplementation on match activities and development of fatigue during a football match. In a randomised, double-blind cross-over design, two experimental football games separated by 7 days were organised between the junior teams of two professional football clubs (17.6 ± 1.1 years (±s), 71.7 ± 6.9 kg, 13.9% ± 5.0% body fat). The players ingested either a capsule of 6 mg · kg^?1 b.w. caffeine or placebo (dextrose) 65 min prior to the matches. Match activities were assessed using the ZXY match analysis system, and a Yo-Yo intermittent recovery test–level 2 (Yo-Yo IR2) was conducted immediately post-game. Heart rate was monitored throughout the game, and blood samples were obtained at baseline, half-time and after the game. There were no differences between caffeine and placebo regarding total distance covered (10,062 ± 916 vs 9854 ± 901 m), high-intensity running (557 ± 178 vs 642 ± 240 m), sprinting distance (109 ± 58 vs 112 ± 69 m) or acceleration counts (123 ± 31 vs 126 ± 24). In both trials, players displayed lower (P < 0.05) values in total distance and acceleration counts in the last 15 min compared to all other 15-min periods of the matches. Post-game Yo-Yo IR2 performance was not different between game trials (caffeine: 829 ± 322 m; placebo 819 ± 289 m). In conclusion, oral caffeine administration does not appear to have an ergogenic effect in young football players during match play.     

The effects of caffeine ingestion on performance time,speed and power during a laboratory-based 1 km cycling time-trial

Abstract

There is little published data in relation to the effects of caffeine upon cycling performance, speed and power in trained cyclists, especially during cycling of ～60 s duration. To address this, eight trained cyclists performed a 1 km time-trial on an electronically braked cycle ergometer under three conditions: after ingestion of 5 mg · kg^?1 caffeine, after ingestion of a placebo, or a control condition. The three time-trials were performed in a randomized order and performance time, mean speed, mean power and peak power were determined. Caffeine ingestion resulted in improved performance time (caffeine vs. placebo vs. control: 71.1 ± 2.0 vs. 73.4 ± 2.3 vs. 73.3 ± 2.7 s; P = 0.02; mean ± s). This change represented a 3.1% (95% confidence interval: 0.7–5.6) improvement compared with the placebo condition. Mean speed was also higher in the caffeine than placebo and control conditions (caffeine vs. placebo vs. control: 50.7 ± 1.4 vs. 49.1 ± 1.5 vs. 49.2 ± 1.7 km · h^?1; P = 0.0005). Mean power increased after caffeine ingestion (caffeine vs. placebo vs. control: 523 ± 43 vs. 505 ± 46 vs. 504 ± 38 W; P = 0.007). Peak power also increased from 864 ± 107 W (placebo) and 830 ± 87 W (control) to 940 ± 83 W after caffeine ingestion (P = 0.027). These results provide support for previous research that found improved performance after caffeine ingestion during short-duration high-intensity exercise. The magnitude of the improvements observed in our study could be due to our use of sport-specific ergometry, a tablet form and trained participants.     

Effects of coffee and caffeine anhydrous on strength and sprint performance

Caffeine and coffee are widely used among active individuals to enhance performance. The purpose of the current study was to compare the effects of acute coffee (COF) and caffeine anhydrous (CAF) intake on strength and sprint performance. Fifty-four resistance-trained males completed strength testing, consisting of one-rep max (1RM) and repetitions to fatigue (RTF) at 80% of 1RM for leg press (LP) and bench press (BP). Participants then completed five, 10-second cycle ergometer sprints separated by one minute of rest. Peak power (PP) and total work (TW) were recorded for each sprint. At least 48 hours later, participants returned and ingested a beverage containing CAF (300?mg flat dose; yielding 3–5?mg/kg bodyweight), COF (8.9?g; 303?mg caffeine), or placebo (PLA; 3.8?g non-caloric flavouring) 30 minutes before testing. LP 1RM was improved more by COF than CAF (p?=?.04), but not PLA (p?=?.99). Significant interactions were not observed for BP 1RM, BP RTF, or LP RTF (p?>?.05). There were no sprint?×?treatment interactions for PP or TW (p?>?.05). 95% confidence intervals revealed a significant improvement in sprint 1 TW for CAF, but not COF or PLA. For PLA, significant reductions were observed in sprint 4 PP, sprint 2 TW, sprint 4 TW, and average TW; significant reductions were not observed with CAF or COF. Neither COF nor CAF improved strength outcomes more than PLA, while both groups attenuated sprint power reductions to a similar degree. Coffee and caffeine anhydrous may be considered suitable pre-exercise caffeine sources for high-intensity exercise.     

Caffeine supplementation and peak anaerobic power output

Abstract

The aim of this study was to investigate the effects of caffeine supplementation on peak anaerobic power output (W_max). Using a counterbalanced, randomised, double-blind, placebo-controlled design, 14 well-trained men completed three trials of a protocol consisting of a series of 6-s cycle ergometer sprints, separated by 5-min passive recovery periods. Sprints were performed at progressively increasing torque factors to determine the peak power/torque relationship and W_max. Apart from Trial 1 (familiarisation), participants ingested a capsule containing 5 mg·kg^?1 of caffeine or placebo, one hour before each trial. The effects of caffeine on blood lactate were investigated using capillary samples taken after each sprint. The torque factor which produced W_max was not significantly different (p ≥ 0.05) between the caffeine (1.15 ± 0.08 N·m·kg^?1) and placebo (1.13 ± 0.10 N·m·kg^?1) trials. There was, however, a significant effect (p < 0.05) of supplementation on W_max, with caffeine producing a higher value (1885 ± 303 W) than placebo (1835 ± 290 W). Analysis of the blood lactate data revealed a significant (p < 0.05) torque factor × supplement interaction with values being significantly higher from the sixth sprint (torque factor 1.0 N·m·kg^?1) onwards following caffeine supplementation. The results of this study confirm previous reports that caffeine supplementation significantly increases blood lactate and W_max. These findings may explain why the majority of previous studies, which have used fixed-torque factors of around 0.75 N·m·kg^?1 and thereby failing to elicit W_max, have failed to find an effect of caffeine on sprinting performance.     

Cognitive,physical and physiological responses of school boy cricketers to a 30-over batting simulation

The purpose of this study was to assess how cognitive and physical performance are affected during a prolonged, fatigue-inducing cricket-batting simulation. Fifteen amateur batters from three Eastern Cape schools in South Africa were recruited (mean ± SD: age 17 ± 0.92 years; stature 1.75 ± 0.07 m; body mass 78.3 ± 13.2 kg). Participants completed a 6-stage, 30-over batting simulation (BATEX^©). During the protocol, there were five periods of cognitive assessment (CogState brief test battery, Melbourne, Australia). The primary outcome measures from each cognitive task were speed and accuracy/error rates. Physiological (heart rate) and physical (sprint times) responses were also recorded. Sprint times deteriorated (d = 0.84; P < 0.01) while physiological responses increased (d = 0.91; P < 0.01) as batting duration increased, with longest times and highest responses occurring in the final stage. Prolonged batting had a large effect on executive task performance (d = 0.85; P = 0.03), and moderate effects on visual attention and vigilance (d = 0.56; P = 0.21) and attention and working memory (d = 0.61; P = 0.11), reducing task performance after 30 overs. Therefore, prolonged batting with repeated shuttle running fatigues amateur batters and adversely affects higher-order cognitive function. This will affect decision-making, response selection, response execution and other batting-related executive processes. We recommend that training should incorporate greater proportions of centre-wicket batting with repeated, high-intensity shuttle running. This will improve batting-related skills and information processing when fatigued, making practice more representative of competition.     

Age-related differences in repeated-sprint ability in highly trained youth football players

Abstract

In this study, we investigated the age-related differences in repeated-sprint ability and blood lactate responses in 134 youth football players. Players from the development programme of a professional club were grouped according to their respective under-age team (U-11 to U-18). Following familiarization, the participants performed a repeated-sprint ability test [6 × 30-m sprints 30 s apart, with active recovery (2.0–2.2 m · s^?1) between sprints]. The test variables were total time, percent sprint decrement, and post-test peak lactate concentration. Total time improved from the U-11 to U-15 age groups (range 33.15 ± 1.84 vs. 27.25 ± 0.82 s), whereas no further significant improvements were evident from U-15 to U-18. No significant differences in percent sprint decrement were reported among groups (range 4.0 ± 1.0% to 5.5 ± 2.1%). Post-test peak lactate increased from one age group to the next (range 7.3 ± 1.8 to 12.6 ± 1.6 mmol · l^?1), but remained constant when adjusted for age-related difference in body mass. Peak lactate concentration was moderately correlated with sprint time (r = 0.70, P > 0.001). Our results suggest that performance in repeated-sprint ability improves during maturation of highly trained youth football players, although a plateau occurs from 15 years of age. In contrast to expectations based on previous suggestions, percent sprint decrement during repeated sprints did not deteriorate with age.     

Validity of a single lactate measure to predict fixed lactate thresholds in athletes

This study aimed to validate the use of a single blood lactate concentration measure taken following a 12 km h^?1 running stage (BLC₁₂) to predict and monitor fixed blood lactate concentration (FBLC) thresholds. Three complementary studies were undertaken. Study I: the relationships between BLC₁₂ and the running speeds at FBLC of 3 mmol L^?1 (S3mM) and 4 mmol L^?1 (S4mM) measured during a multistage running field test were examined in 136 elite athletes. Study II: data from 30 athletes tested one year apart were used to test the predictive capacity of the equations obtained in Study I. Study III: 80 athletes were tested before and after an intensified training period to examine whether training-induced changes in FBLC thresholds could be predicted and monitored by BLC₁₂. Study I: BLC₁₂ was significantly (P < 0.001) and inversely related to S3mM (R² = 0.89) and S4mM (R² = 0.95). Study II: prediction models yielded robust correlations between the estimated and measured FBLC thresholds (r = 0.94–0.99; P < 0.001). Study III: estimated changes predicted actual training-induced changes in FBLC thresholds (r = 0.81–0.91; P < 0.001). This study gives empirical support to use a single lactate measure during a sub-maximal running field test as a simple, low-cost and practical alternative to FBLC thresholds in athletes.     

The immune response to short-duration exercise in trained,eumenorrhoeic women

Few studies have characterised the immune response to exercise of different intensities and durations in women. In those that have, baseline hormone levels and training status were not always adequately controlled for. Here, leucocyte and cytokine profiles of 11 aerobically trained, eumenorrhoeic females (33 ± 5 years) in the early follicular phase of the menstrual cycle were characterised after 30-min exercise at 3 intensities: 90% lactate threshold (LT), LT, and 110% LT. Proposed cytokine response mediators were quantified: plasma lactate and basal oestradiol concentrations. Intensity-dependent increases occurred in total white blood cells and lymphocyte counts (P < 0.001). Elevated plasma IL-6 and IL-1ra concentrations post-exercise [F = 12.38, P < 0.01 and F = 7.65, P < 0.05, respectively] were not intensity-dependent, indicating that cytokine release may be better associated with exercise duration than intensity in trained women. Changes in plasma IL-1ra and basal oestradiol (ρ = ?0.893, P < 0.01) were correlated at intensities above LT only. These findings suggest a role for plasma sex hormones in moderating the exercise-induced immune response in women. However, the associations observed did not account for the magnitude of the cytokine response observed, and future studies should explore contributions of other potential mediators following short-duration exercise.     

Relationships among field-test measures and physical match performance in elite-standard soccer referees

Abstract

The aim of this study was to assess the extent to which measures derived from the new FIFA referees’ fitness tests can be used to monitor a referee's match-related physical capacity. Match-analysis data were collected (Prozone®, Leeds, UK) from 17 soccer referees for 5.0 (s = 1.7) FA Premier League matches per referee during the first 4 months of the 2007–08 season. Physical match performance categories included total distance covered, high-intensity running distance (speed >5.5 m · s^?1), and sprinting distance (>7.0 m · s^?1). The two tests were a 6 × 40-m sprint test and a 150-m interval test. Heart rate demand was correlated with total match distance covered (r = ?0.70, P = 0.002) and high-intensity running (r = ?0.57, P = 0.018) in the interval test. The fastest 40-m sprint was related to total distance covered (r = ?0.69, P = 0.002), high-intensity running (r = ?0.76, P < 0.001), and sprinting distance (r = ?0.75, P = 0.001), while mean time for the 40-m sprints was related to total distance covered (r = ?0.70, P = 0.002), high-intensity running (r = ?0.77, P < 0.001), and sprinting distance (r = ?0.77, P < 0.001). The referees who recorded the best interval-test heart rate demand and fastest 40-m time produced the best physical match performances. However, only the sprint test and in particular the fastest 40-m time had appropriate construct validity for the physical assessment of soccer referees.     

Effects of caffeine on time trial performance in sedentary men

Abstract

It is not known if ergogenic effects of caffeine ingestion in athletic groups occur in the sedentary. To investigate this, we used a counterbalanced, double-blind, crossover design to examine the effects of caffeine ingestion (6 mg · kg^?1 body-mass) on exercise performance, substrate utilisation and perceived exertion during 30 minutes of self-paced stationary cycling in sedentary men. Participants performed two trials, one week apart, after ingestion of either caffeine or placebo one hour before exercise. Participants were instructed to cycle as quickly as they could during each trial. External work (J · kg^?1) after caffeine ingestion was greater than after placebo (P = 0.001, effect size [ES] = 0.3). Further, heart rate, oxygen uptake and energy expenditure during exercise were greater after caffeine ingestion (P = 0.031, ES = 0.4; P = 0.009, ES = 0.3 and P = 0.018, ES = 0.3; respectively), whereas ratings of perceived exertion and respiratory exchange ratio values did not differ between trials (P = 0.877, ES = 0.1; P = 0.760, ES = 0.1; respectively). The ability to do more exercise after caffeine ingestion, without an accompanying increase in effort sensation, could motivate sedentary men to participate in exercise more often and so reduce adverse effects of inactivity on health.     

Assessment of vitamin D concentration in non-supplemented professional athletes and healthy adults during the winter months in the UK: implications for skeletal muscle function

Abstract

The current study implemented a two-part design to (1) assess the vitamin D concentration of a large cohort of non-vitamin D supplemented UK-based athletes and 30 age-matched healthy non-athletes and (2) to examine the effects of 5000 IU · day^?1 vitamin D₃ supplementation for 8-weeks on musculoskeletal performance in a placebo controlled trial. Vitamin D concentration was determined as severely deficient if serum 25(OH)D < 12.5 nmol · l^?1, deficient 12.5–30 nmol · l^?1 and inadequate 30–50 nmol · l^?1. We demonstrate that 62% of the athletes (38/61) and 73% of the controls (22/30) exhibited serum total 25(OH)D < 50 nmol · l^?1. Additionally, vitamin D supplementation increased serum total 25(OH)D from baseline (mean ± SD = 29 ± 25 to 103 ± 25 nmol · l^?1, P = 0.0028), whereas the placebo showed no significant change (53 ± 29 to 74 ± 24 nmol · l^?1, P = 0.12). There was a significant increase in 10 m sprint times (P = 0.008) and vertical-jump (P = 0.008) in the vitamin D group whereas the placebo showed no change (P = 0.587 and P = 0.204 respectively). The current data supports previous findings that athletes living at Northerly latitudes (UK = 53° N) exhibit inadequate vitamin D concentrations (<50 nmol · l^?1). Additionally the data suggests that inadequate vitamin D concentration is detrimental to musculoskeletal performance in athletes. Future studies using larger athletic groups are now warranted.     

Carbohydrate mouth rinse does not affect performance during a 60-min running race in women

This study examined the effect of carbohydrate mouth rinsing on endurance running performance in women. Fifteen female recreational endurance runners, who used no oral contraceptives, ran two races of 1-h duration on an indoor track (216-m length) at 18:00 h after an 8-h fast with a 7-days interval between races, corresponding to the 3^rd-10th day of each premenopausal runner’s menstrual cycle, or any day for the postmenopausal runners. In a double-blind random order, participants rinsed their mouth with 25 ml of either a 6.4% carbohydrate (RCHO) or a placebo solution (RP). No fluid was ingested during exercise. Serum 17β-Εstradiol (P = 0.59) and Progesterone (P = 0.35) did not differ between treatments. There was no difference in 1-hour running performance (RCHO: 10,621.88 ± 205.98 m vs. RP: 10,454.00 ± 206.64 m; t = 1.784, P = 0.096). Furthermore, the mean percentage effect (±99%CI) of RCHO relative to RP, 1.67% (?1.1% to 4.4%), and Cohen’s effect size (d = 0.21) support a trivial outcome of RCHO for total distance covered. In conclusion, carbohydrate mouth rinsing did not improve 60-min track running performance in female recreational runners competing in a low ovarian hormone condition, after an 8-h fast and when no fluid was ingested during exercise.     

Leukocyte telomere length and mortality among U.S. adults: Effect modification by physical activity behaviour

The purpose of this study was to examine the association between leukocyte telomere length (LTL) and mortality (outcome variable), with consideration by physical activity behaviour. Data from the 1999–2002 National Health and Nutrition Examination Survey were employed (N = 6,611; 20–85 yrs), with follow-up mortality assessment through 31 December 2006. DNA was extracted from whole blood to assess LTL via quantitative polymerase chain reaction. Compared to those in the first LTL tertile, the adjusted hazard ratio for all-cause mortality for those in the 2^nd and 3^rd LTL tertiles, respectively, was 0.82 (95% CI: 0.60–1.12; P = .22) and 0.76 (95% CI: 0.50–1.14; P = .18). However, after adjustments, LTL tertile 3 (vs. 1) was associated with all-cause mortality (HR = 0.37; 95% CI: 0.14–0.93; P = .03) for those who engaged in moderate-intensity exercise. Similarly, LTL was associated with CVD-specific mortality for those who engaged in moderate-intensity exercise (HR = 0.17; 95% CI: 0.04–0.73; P = .02). Longer telomeres are associated with increased survival, particularly among men and those who are active, underscoring the importance of promotion of physical activity behaviour.