Ionizing Radiation Induces HMGB1 Cytoplasmic Translocation and Extracellular Release

Objective A nucleosomal protein,HMGBI,can be secreted by activated immune cells or passively released by dying cells,thereby amplifying rigorous inflammatory responses.In this study we aimed to test the possibility that radiation similarly induces cytoplasmic HMGB1 translocation and release.Methods Human skin fibroblast (GM0639) and bronchial epithelial (16HBE) cells and rats were exposed to X-ray radiation,and HMGB1 translocation and release were then assessed by immunocytochemistry and immunoassay,respectively.Results At a wide dose range(4.0-12.0 Gy),X-ray radiation induced a dramatic cytoplasmic HMGB1 translocation,and triggered a time-and dose-dependent HMGB1 release both in vitro and in vivo.The radiation-mediated HMGB1 release was also associated with noticeable chromosomal DNA damage and loss of cell viability.Conclusions Radiation induces HMGB1 cytoplasmic translocation and extracellular release through active secretion and passive leakage processes.     

HPV16 E6/E7 Negatively Affect Radiosensitivity of Lung Cancer Cells

Objective Lung cancer cells associated with radioresistance are likely to give rise to local recurrence and distant metastatic relapse,but little is known about its underlying mechanisms.In the present paper,the effects of the HPV16 E6 and HPV16 E7 oncoprotein on the radiosensitivity of lung cancer cell lines were investigated.Methods The HPV16 E6 or HPV16 E7 oncoprotein was expressed by a transient transfection with pcDNA3-HPV16 E6 or pcDNA3-HPV16 E7 expression vector.Human lung cancer H2179 cells and mouse lung cancer Lewis cells were exposed to a γ-ray radiation source,cellular survival was evaluated by using a colony formation assay.The expression of HPV16 oncoproteins E6/E7,extracellular signal-regulated kinases 1/2(ERK1/2) and AKT signaling was determined by Western blot assay.VEGF secretion was determined by ELISA.Results Both HPV16 oncoproteins E6 and E7 significantly decreased radiosensitivity of H2179 cells,associated with a promotion of the ERK1/2 and AKT phosphorylation.A decrease of reactive oxygen species(ROS) and an increase of VEGF levels were observed in the cells expressing the HPV16 oncoproteins E6 and E7.Furthermore,a similar reduction of radiosensitivity mediated by the HPV16 oncoproteins E6 and E7 was also observed in a mouse lung cancer Lewis cells.Conclusion The findings indicate that the HPV16 oncoproteins E6 and E7 negatively affects susceptibility of lung cancer cells to radiotherapy via regulation of the ERK1/2 and Akt signaling pathway and VEGF expression.     

先天性右侧腓骨发育畸形1例

1 病历简介 患者，男，16岁。右下肢跛行3年余，但未影响正常生活，未予足够重视，现感行走时跛行加重、偶有酸痛而入院。查体：生命体征正常，神志清楚，头颅、五官、躯体检查未见异常，发育良好、无畸形，肢体活动尚自如；心肺腹部检查未发现异常；神经系统检查阴性。专科检查：右侧小腿相对左侧稍细，小腿围较健侧约小5．2cm，触及轻压痛，皮温与健侧相同。X线右小腿正侧位片（图1）示：     

优生从择偶开始

家庭成员的寿命宜长很可能有人会提出异议,挑对象应当看本人,与家庭成员何干?其实不然,生物学家已经发现,生物的生存期取决于遗传基因。换言之,一个人能活多久在相当程度上与其父母及家庭成员有关,长寿家庭成员的体内很可能存     

高渗氯化钠右旋糖酐70注射液的血管刺激性溶血性和过敏性研究

目的 研究高渗氯化钠右旋糖酐70注射液(Hypertonic saline dextran70 injection,HSD)是否具有血管刺激性、溶血性和过敏性作用.方法 对中试产品进行了血管刺激性实验、全身过敏实验和溶血实验.结果 HSD对血管没有明显的刺激作用;不引起豚鼠的过敏反应;不引起溶血和红细胞凝集作用.结论 制剂的安全性得到确证.     

3种抗缺氧药物对筑路工人急进海拔4 600m不同时间血红蛋白的影响

目的:探讨三种抗缺氧药物对筑路工人急进高海拔地区不同时间血红蛋白的影响.方法:受试者随机分为服药组A组(复方1号)、B组(复方2号)、C组(复方3号)和对照组(D组),每组(23～24)人.启程时开始服药,每天早、中、晚各服药一次,连续服药3周.到达海拔4 600m施工现场第1天、3天、7天、15天进行血红蛋白测定,与服药前血红蛋白比较.结果:进驻后第一天各组血红蛋白与服药前组内比较,差别有非常显著性(P＜0.01);第15天复方2号组、安慰剂组与服药前组内比较,差别有显著性(P＜0.05).第1天与第3天、第15天各组组内比较,差别有显著性(P＜0.05);第一天与第7天比较,除复方2号组外其余各组组内比较,均差别有非常显著性(P＜0.01);第3天与第15天复方1号组、复方3号组组内比较,差别有显著性(P＜0.05).结论:三种抗缺氧药物均不同程度改善红细胞携氧功能,以复方1号、复方3号效果较好.     

青年飞行员脑出血死亡一例

一、临床资料患者男性,25岁,轰炸机飞行员,飞行时间130 h.以发热、咽痛3 d,加重伴恶心、呕吐1 d于2009年12月20日入院.患者受凉后于12月17日晚出现发热(未测体温)、咽痛,伴全身酸困不适,偶有轻咳,无痰,无关节疼痛、胸痛等,自服"白加黑、急支糖浆"等药物治疗,18日晨起,发热症状消失,咽痛有所减轻;下午症状有所反复,继续口服上述药物治疗.     

敬告作者

本刊在2008年继续大力推进E-mail投稿,同时还接受经邮局寄的纸样稿件(同一稿件不要用E-mail与邮局同时投稿)。文章务必用Word格式打印,并附3.5寸软盘。发E-mail者可不交软盘。     

家长该如何应对小儿腹泻?

小儿腹泻是以大便次数增多、粪便稀薄如水样为特征的一种常见病。该病常年都可发生,以夏秋季节较为多见,秋冬两季发生的小儿腹泻易酿成流行,婴幼儿发病率较高。引起婴幼儿腹泻(特别是急性腹泻)的常见原因是感染,包括细菌感染和病毒感染,常见的病原菌有痢疾杆菌、沙门氏菌及轮状病毒。以上病原菌可刺激肠道黏膜,使其分泌增加、吸收能力降低,     

执著缘于热爱——访磁共振专家吴仁华

吴仁华 1959年生,江西大余县人.医学博士,教授,博士生导师,汕头大学医学院医学影像学科负责人,汕头大学医学院学术委员会成员,第二附属医院医学影像学教研室主任,脑功能研究室主任.2003年、2004年国家自然科学基金生命科学部评审(二审)人员,中华医学会放射学会神经组全国委员,<中华放射学杂志>编委,(AJR)审稿员,美国放射学会(ARRS)会员,国际电与电子工程学会(IEEE)和生物医学工程学会(EMBS)会员,国际脑功能成像学会(OHBM)会员并且是The Scientific Advisory Board for HBM2006成员.     

隆重纪念汪绍训教授诞辰100周年

2007年是我国放射学奠基人,著名临床放射学家和医学教育家汪绍训教授诞辰100周年.为纪念汪老百年诞辰,全国人大常委副委员长、中国科协主席韩启德院士题词"传承宗师伟业,创新影像医学",北大医院影像科和中国集邮总公司联合发行纪念邮折一套.     

CT引导颞下窝肿瘤穿刺活检1例

患者 女,25岁,左面颊疼痛3年.患者3年前出现左面颊疼痛,CT检查发现左颞下窝翼内肌处有约3 cm×4 cm肿块,密度基本均匀,与周围肌肉组织分界欠清,颅底骨未见破坏,考虑为良性肿瘤.     

《请您诊断》病例16答案:腹膜促纤维组织增生性小细胞肿瘤一例

病例资料患者,男,25岁,半年前开始反复出现胸闷,在深吸气后加重,可自行缓解,常在半夜出汗。12天前无明显诱因出现腹部不适伴腹胀、乏力而就诊。查体:腹部膨隆,下腹部可触及大小不等坚硬包块,左侧腹股沟区可及3～4枚黄豆大小淋巴结,可活动,肝、脾触诊不满意,移动性浊音( )。实验     

肺癌支气管动脉介入治疗的现状

肺癌是严重危害人类健康的主要恶性肿瘤.由于其生物学特性,目前临床70%的病人一旦确诊已属晚期,能够手术的几率已不大,即使配合放射治疗及化学药物治疗,总的效果仍不满意,5年生存率维持在25%～30%之间.     

飞行人员恶性肿瘤术后安全放飞指征的临床研究

目的 研究和探讨我国飞行人员恶性肿瘤手术后放飞的安全标准. 方法 回顾分析38年间,经病理检查确诊为恶性肿瘤并进行外科手术的飞行人员病例资料,全组25例飞行人员恶性肿瘤术后放飞13例. 结果 本组13例恶性肿瘤术后放飞的飞行人员中,甲状腺癌4例、结肠癌3例、膀胱癌2例,胃癌、胰腺囊腺瘤、乳腺癌和耻骨软骨肉瘤各1例.经手术治疗及术后辅助治疗,13例病人机体状况恢复良好,鉴定结论 :飞行合格.术后随访3～38年,飞行时间30～2000 h,飞行状态良好. 结论 部分患恶性肿瘤的飞行人员,通过仔细认真的手术、正规的术后辅助治疗和护理、循序渐进的体能锻炼、定期严格的健康体检,可以达到飞行合格.作者提出了适合我国飞行人员恶性肿瘤术后恢复飞行的基本标准. Abstract： Objective To study and discuss the safety standard of flying qualification for the postoperative flying personnel with malignant tumor. Methods Related case history in past 38 years was retrospectively reviewed and sought 13 flying qualified cases out of 25 postoperative flying personnel with malignant tumor. Results Among 13 qualified cases there were 4 cases of thyroid carcinoma, 3 cases of colon carcinoma, 2 cases of bladder carcinoma and 4 cases respectively were gastric cancer, cystadenoma of pancreas, breast carcinoma and pubis osteochondrosarcoma. Those 13 cases were cured by operation and other adjuvant treatments and were diagnosed as well recovered. Since they were qualified for flying, the 3-38 years follow-up showed that they had fitted in flying for 30-2000 h. Conclusions Some flying personnel with malignant tumor would be qualified for flying by operation, proper adjuvant treatments and regular nursing, as well as the progressive physical exercise and under regular rigorous physical examination. The paper also puts forward a basic standard that suited for Chinese military flying personnel with malignant tumor.     

低温保存动脉的粘弹性力学评价指标的初步建立

目的 建立低温保存动脉的粘弹性力学评价指标.方法 使用动态力学分析仪(DMA-2980, TA Instruments, New Castle, Delaware, USA) 比较研究不同低温保存方案下动脉蠕变行为与新鲜对照组的差异.结果 低温保存动脉相对新鲜对照组而言,其粘弹性均有损失;随降温速率的增加(1.5, 5, 10℃/min),其粘弹性损失趋于增加,即1.5℃/min 为本研究中最佳降温速率.结论 粘弹性力学性能是低温保存动脉的潜在的重要评价指标.     

免疫人体清除"异己"的"特殊反应"

人们发现:在传染病流行过程中得病而幸免于难的人会对该病获得抵抗力,当此种传染病再次流行时他可以安然无恙。可能由此受到启发,早在公元10世纪,我国就已经用接种人痘的方法预防天花。约800年后,即1798年,英国医生发明了牛痘接种法,逐步取代了不够安全有效的人痘接种。经过多年不懈的努力,1979年10月26日,世界卫生组织正式宣布全球消灭了天花,这开创了应用免疫学方法在全球范围内消灭烈性传染病的先河。     

骨细胞在力学信号感受过程中的作用

目的 综述骨细胞在力学信号感受过程中的作用.资料来源与选择国内外公开发表的相关学术论文及研究报告.资料引用引用文献资料57篇.资料综合主要对骨细胞的生物学特点,骨细胞与成骨细胞之间的差异,骨细胞的特殊结构在力传导中的作用,骨细胞是骨组织的力学感受器,骨细胞力学感受过程中的信息传递,骨细胞与失重性骨丢失等6个方面进行综述.结论 骨细胞是骨组织中的力学感受器,其感受机械刺激,通过细胞网络传递信号,在骨重塑过程中调节成骨细胞和破骨细胞的功能. Abstract： Objective To summarize the roles of osteocytes in bone mechanosensation. Literature resource and selection Related papers and reviews that published at home and abroad. Literature quotation Fifty-seven papers were cited. Literature synthesis Such subjects as biological characteristics of osteocytes, differences between osteocytes and osteoblasts, the roles of special structures of osteocytes in mechanotransduction, the mechanosensors of bone, signal transmission during mechanosensation, the relationship of osteocytes and weightless caused bone loss were reviewed and summarized. Conclusion Osteocytes are the mechanosensors in bone. When mechanical stimuli are sensed, osteocytes regulate the functions of osteoblasts and osteoclasts by the cellular network transmission in the process of bone remodeling.