薄层色谱法纯化卟啉锰修饰的TFO

目的 筛选分离纯化卟啉锰-TFO-吖啶化合物的简单有效的方法。方法 以固相亚磷酰胺法合成吖啶，6碳氨基连接臂修饰的TFO，并以温和法切割和脱保护；四苯基卟啉四羧酸经金属化，酯化后，与上述TFO偶联；分别以变性聚丙酰胺凝胶电泳，寡核苷酸纯化柱，薄层色谱法分离纯化偶联反应产物。结果 以凝胶电泳分离偶联反应产物时，偶联反应产物带，卟啉锰活性酯带位于同一水平，未分离出独立的卟啉锰-TFO-吖啶化合物带，以寡核苷酸纯化柱分离偶联反应产物时，紫外分光光度仪检测结果显示，回收物仍为卟啉锰-TFO-吖啶化合物与游离的卟啉锰活性酯的混合物。以薄层层析分离偶联反应产物时，在卟啉锰活性单酯带后，出现一条绿色月牙型带，紫外光光谱分析显示，此带回收物同时具有寡核苷酸和卟啉锰的特征吸收峰（UVλmax=260,468nm)。质谱分析结果证实，回收物分子量实测值与卟啉锰-TFO-吖啶化合物理论一致。结论 薄层色谱法可简单有效分离纯化卟啉锰-TFO-吖啶化合物。     

新型尾式卟啉的制备、表征及其与DNA相互作用的初步研究

目的合成1个新型尾式卟啉化合物（目标化合物1）,并对其与小牛胸腺DNA（CT-DNA）的相互作用方式进行初步研究。方法以吡咯、对羟基苯甲醛和苯甲醛为原料,制备得到以柔性碳链连接的目标化合物1,采用MS1、HNMR以及UV-vis等对化合物进行表征;并采用紫外光谱、荧光光谱以及黏度实验初步研究目标化合物1与CT-DNA的相互作用。结果目标化合物1的电喷雾质谱（ESI-MS）在997.4出现一个归属于[M＋H]＋的分子离子峰,实验结果与理论计算一致;目标化合物1的电子吸收光谱分别在419 nm出现一个归属于卟啉环的特征Soret吸收,在500~700 nm之间出现归属于卟啉环的特征Q-带吸收。结论目标化合物1能够以插入方式与DNA分子发生结合。     

5-吡啶基-10,15,20-三甲苯基卟啉及其类似物的合成与表征

目的与方法以吡咯、对甲基苯甲醛、对甲氧基苯甲醛和醛基吡啶为原料,制备3个不对称卟啉化合物：5-（3′-吡啶基）-10,15,20-三甲苯基卟啉（1）,5-（3′-吡啶基）-10,15,20-三甲氧苯基卟啉（2）,5-（4′-吡啶基）-10,15,20-三甲氧苯基卟啉（3）。采用元素分析、电喷雾质谱、核磁共振以及电子吸收光谱和荧光发射光谱对目标化合物进行表征。结果与结论目标化合物1、2、3的电喷雾质谱（ESI-MS）分别在658.7、706.5和706.5出现一个归属于[M＋H]＋的分子离子峰,实验结果与理论计算基本一致;目标化合物1、2、3的电子吸收光谱分别在419、422和422 nm出现一个归属于卟啉环的特征Soret吸收,在450-700 nm之间出现归属于卟啉环的特征Q-带吸收。     

复方补骨脂口服液中补骨脂素的含量测定

根椐收载的验方,我们制成复方补骨脂口服液用于治疗五更泄泻、便溏腹痛。方中主要生药有补骨脂、肉豆蔻等。补骨脂温肾暖脾为君,肉豆蔻为臣,其余为佐使。补骨脂生药中所含补骨脂素为呋喃香豆素化合物,在紫外光区有吸收,在一定浓度范围内呈线性关系。本法采用薄层层析,苯—氯仿—乙酸乙脂为展开剂,展开后在紫外灯下,将补骨脂素斑点刮取,用乙醇洗脱完全,在328nm 处     

2种烷基取代吖啶酮磺酰氯紫外荧光衍生剂的合成及其光谱性质

目的以吖啶酮为原料,合成N-甲基吖啶酮-3-磺酰氯(MASC)和N-乙基吖啶酮-3-磺酰氯(EASC)2种新型紫外荧光衍生剂。方法经N位上烷基化修饰和氯磺化合成MASC和EASC,用紫外、核磁共振、质谱等技术对其进行结构表征,并将MASC、EASC与衍生剂丹磺酰氯(DNS-Cl)的紫外吸收及荧光强度相比较。结果通过紫外、核磁共振、质谱等技术证明了二者即为目标化合物,EASC、MASC与DNS-Cl的摩尔吸光系数之比分别为UVEASC-257 nm/UVDNS-Cl-323 nm=7.57、UVMASC-257 nm/UVDNA-Cl-323 nm=7.75,荧光量子效率之比分别为Φf(EASC)/Φf(DNS-Cl)=4.70、Φf(EASC)/Φf(DNS-Cl)=5.42,与二乙胺衍生的衍生物荧光强度之比分别为If(EASC)/If(DNS-Cl)=97.82、If(MASC)/If(DNS-Cl)=103.38。结论EASC和MASC有较强的紫外吸收与荧光强度,有望用于含氨基类化合物的痕量测定。     

石榴花、蒂、皮微量元素、薄层层析及紫外光谱分析

目的：通过测定石榴花、蒂、皮的微量元素含量、对照薄层层析及紫外吸收光谱，探讨其化学成分的相对含量及差异。方法：分别测定石榴花、蒂、皮微量元素含量，并作对照；对3个样品分别在两种不同展开体系中进行对照薄层层析；分别测定其紫外吸收光谱。结果：石榴的花、蒂、皮3部分的微量元素含量相差较大，其中花和皮的微量元素含量相对较高，而花中更为明显。不同展开体系的对照薄层层析显示石榴花展开后斑点较多，皮次之，蒂最少。紫外吸收光谱图在约λ220nm及λ260nm的两个主要吸收峰的吸收值，变为石榴花的最大，皮的次之，蒂的最小。结论：实验结果预示石榴花中的化学成分较为丰富，有望进一步深入研究。     

河南鼠尾草化学成分的研究

河南鼠尾草SalviahonaniaL．H．Bailey系唇形科鼠尾草属植物，主要分布在湖北、河南两省，是一种民间常用草药，其功效为活血调经，祛瘀止痛。将河南鼠尾草的丙酮提取物经氯访一水分配所得氯仿提取物，进行离心薄层层析及制备薄层层析，分得3个化合物，经鉴定分别为丹参酿-ⅡA、隐丹参酮和β-谷甾醇。所有成分均系首次从该植物中分得。1仪器与试剂熔点系用XRC－1显微熔点仅测定，未校正。紫外光谱用SchimadzuUV－2100仪测定。红外光谱用NICOLETFT－IR710仪测定。核磁共振谱用JEOL－270MHZ仪测定。质谱用JEOLD300仪测定。离心…     

异紫杉脂素的酶法糖基化

目的 以异紫杉脂素为例，探索酶法糖基化的可行性，为其他具有重要生理活性的天然化合物提供可行的生物转化方法。方法 利用半乳糖苷酶转糖基活性对异紫杉脂素进行了糖基化修饰。结果 通过薄层层析，质谱，红外光谱，^1HNMR,^13CNMR数据显示产物即是异紫杉脂素半乳糖苷。结论 E.coli和脆壁酵母来源的β-半乳糖苷酶可以对异紫杉脂素进行糖基化修饰。     

5,10,15,20—四（邻（5—氟尿嘧啶—1）乙酰苯胺）苯基卟啉的合成

报道了新型抗癌药物５，１０，１５，２０－四（邻（５－氟尿嘧啶－１）乙酰苯胺）苯基卟啉及其顺式异构体的合成方法，产率分别为１８．１％和１６．８％，这些化合物的结构经紫外光谱，红外光谱，核磁共振谱，质谱及元素分析确证。     

5,10,15,20-四[邻(5-氟尿嘧啶-1)乙酰苯胺]苯基卟啉的合成

报道了新型抗癌药物５，１０，１５，２０－四［邻（５－氟尿嘧啶－１）乙酰苯胺］苯基卟啉及其顺式异构体的合成方法，产率分别为１８．１％和１６．８％，这些化合物的结构经紫外光谱、红外光谱、核磁共振谱、质谱及元素分析确证     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.