细胞因子对HCV基因疫苗诱生免疫反应的增强作用

目的 探讨细胞因子对丙型肝炎病毒（ＨＣＶ）核心（Ｃ）基因疫苗诱生免疫应答强度的增强作用。方法 将包含ＨＣＶ－Ｃ蛋白的基因片段插入真核表达载体ｐｃＤＮＡ３中,构建重组质料ｐｃＤＮＡＨＣＶ－Ｃ,将此重组质粒单独或与包含鼠ＩＬ－２或ＩＬ－１２基因的表达质粒共免疫ＢＡＬＢ／ｃ小鼠,ＥＬＩＳＡ法检测ＨＣＶ Ｃ特异性抗体产生情况,以ｐｃＤＮＡＨＣＶ－Ｃ转梁小鼠ＳＰ２／０细胞,表达ＨＣＶ Ｃ抗原为靶细胞,进行     

柯萨奇病毒B组3型VP1基因真核表达重组质粒诱导小鼠中和？…

以构建的柯萨奇病毒Ｂ组３型（ＣＶＢ３）ＶＰ１基因的真核表达重组质粒ｐＣＥＰ４－ＣＶＢ３ＶＰ１作为侯选的基因疫苗，评价其诱导小鼠产生的中和抗体反应。大量制备并提纯ｐＣＥＰ４－ＣＶＢ３ＶＰ１ ＤＮＡ，用其接种ＢＡＬＢ／Ｃ小鼠，取血清进行病毒中和试验。结果证明其可诱导小鼠机体产生特异性中和抗体，并能中和ＣＶＢ３毒力，起到基因疫苗的预防作用。     

基因免疫诱导小鼠产生抗丙型肝炎病毒E2糖蛋白抗体

目的：通过基因免疫诱导ＢＡＬＢ／ｃ小鼠产生抗Ⅲ型中国株ＨＣＶ来源Ｅ２糖蛋白的体液免疫应答。方法：质粒ｐ３Ｗ１４在ＮＩＨ３Ｔ３细胞中能够表达Ｅ２糖蛋白，纯化该质粒并用于ＢＡＬＢ／ｃ小鼠直接肌注，于加强注射后不同时相采血，以重组的Ｅ２糖蛋白检测特异性抗Ｅ２抗体。结果：接种ｐ３Ｗ１４质粒的小鼠中可检出抗Ｅ２抗体（５／６），抗体滴度１∶１５～１∶１２０。结论：采用含Ｅ２／ＮＳ１基因的质粒ＤＮＡ免疫，成功地诱导小鼠产生抗Ⅲ型株来源的Ｅ２抗体。     

基因免疫诱导小鼠产生抗丙型肝炎病毒E2糖蛋白抗体

目的：通过基因免疫诱导ＢＡＬＢ／ｃ小鼠产生抗Ⅲ型中国株ＨＣＶ来源Ｆ２糖蛋白的体液免疫应答，方法：质粒ｐ３Ｗ１４在ＮＩＨ３Ｔ３细胞中能够表达Ｅ２糖蛋白，纯化该质粒并用于ＢＡＬＢ／ｃ小鼠直接肌注，于加强注射后不同时相采血，以重组的Ｅ２糖蛋白检测特异性抗Ｅ２抗体。结果；接种ｐ３Ｗ１４质粒的小鼠中检出抗Ｅ２抗体（５／６），抗体滴度１：１５～１：１２０。结论；采用含Ｅ２／ＮＳ１基因的质粒ＤＮＡ免疫，成功地诱     

柯萨奇病毒B组3型VP1基因真核表达重组质粒诱导小鼠中和抗体反应

以构建的柯萨奇病毒Ｂ 组３ 型（ＣＶＢ３）ＶＰ１ 基因的真核表达重组质粒ｐＣＥＰ４ＣＶＢ３ＶＰ１ 作为候选的基因疫苗,评价其诱导小鼠产生的中和抗体反应。大量制备并提纯ｐＣＥＰ４ＣＶＢ３ＶＰ１ ＤＮＡ,用其接种ＢＡＬＢ／Ｃ小鼠,取血清进行病毒中和试验。结果证明其可诱导小鼠机体产生特异性中和抗体,并能中和ＣＶＢ３ 毒力,起到基因疫苗的预防作用。     

丙肝核酸免疫效果初步观察

目的 观察丙肝核酸疫苗的免疫效果。方法 用丙肝病毒Ｃ＋Ｅ１区基因的真核表达重组质粒ｐＳＶＬ－ＨＣＶ／Ｃ＋Ｅ１免疫蛇毒预处理的ＢＡＬＢ／Ｃ和Ｃ５７ＢＬ小鼠，两周后采用ＥＬＩＳＡ法开始检测抗体滴度。结果 真核表达重组质粒ｐＳＶＬ－ＨＣＶ／Ｃ＋Ｅ１免疫能诱导习免疫应答，产生高水平的特异抗体。特别是Ｃ５７ＢＬ小鼠抗体反应最好，初次免疫就能产生高水平的特异抗体，加强后能持续数周，最高滴度ＯＤ值达１．４５。结     

戊型肝炎病毒（HEV）基因疫苗免疫小鼠的初步研究

本研究将含ＨＥＶＯＲＦ３ｃＤＮＡ的真核表达质粒作为基因疫苗直接注射到ＢＡＬＢ／ｃ小鼠骨胳肌内，引发了实验小鼠特异性抗－ＨＥＶＩｇＧ反应。小鼠经２次基因疫苗接种或１次基因疫苗，然后用ＨＥＶ重组蛋白加强免疫后，血清抗－ＨＥＶＩｇＧ滴度较接种１次基因疫苗组明显升高。     

戊型肝炎病毒（HEV）基因疫苗免疫小鼠的初步研究

本研究将含ＨＥＶ ＯＲＦ３ ｃＤＮＡ的真核表达质粒作为基因疫苗直接注射到ＢＡＬＢ／ｃ小鼠骨骼肌内，引发了实验小鼠特异性抗－ＨＥＶ ＩｇＧ反应。小鼠经２次基因疫苗接种或１次基因疫苗，然后用ＨＥＶ重组蛋白加强免疫后，血清抗－ＨＥＶ ＩｇＧ滴度较接种１次基因疫苗组明显升高。     

佐剂CpG ODN对HBV基因疫苗诱导抗体产生的影响

目的 探讨人工合成的ＣｐＧ ＯＤＮ作为佐剂对乙肝病毒基因疫苗诱导小鼠产生抗ＨＢｓ的影响。方法 构建编码乙型肝炎病毒（ＨＢＶ）表面抗原蛋白Ｓ的重组真核表达质粒ｐＣＲ３．１－Ｓ作为ＨＢＶ基因疫苗,人工合成含ＣｐＧ ｍｏｔｉｆ的硫代磷酸寡核苷酸作为佐剂,以ＢＡＬＢ／ｃ小鼠作为实验动物进行免疫接种,采用ＥＬＩＳＡ法检测免疫小鼠的抗ＨＢｓ应答。结果 与生理盐水作为佐剂组相比较,应用ＣｐＧ ＤＯＮ组小鼠产生更     

柯萨奇B组病毒3型VP1基因疫苗免疫效果的研究

目的 为研制防治病毒性心肌炎的基因疫苗打下基础。 方法 以带有ＣＶＢ３Ｐ１、Ｐ２区核酸序列的ｐＧＥＭ质粒为模板，用ＰＣＲ方法克隆ＣＶＢ３ＶＰ１基因ｃＤＮＡ，插入真核表达载体ｐｃＤＮＡ３中的ＨｉｎｄⅢ和ＸｂａＩ位点之间，构建成重组质粒。经大量扩增纯化后，肌肉注射免疫ＢＡＬＢ／ｃ小鼠３次，用ＣＶＢ３毒株攻击小鼠，取血及脾细胞检测其免疫效果。 结果 小鼠经３次免疫后，虽未测出明显免疫应答指标，但诱导了Ｔ     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.