长乐、福安鱼露致突变性实验初步结果

从福建长乐、福安两县采集鱼露,用Ames方法检测其致突变性。结果表明,未经过亚硝化的鱼露,其致突变性试验均为阴性;经过亚硝化的鱼露仍有部份致突变性;亚硝化鱼露亚硝酸钠浓度与TA100回变菌落数呈正相关;维生素C有抑制鱼露致突变性且其浓度与TA100回变菌落数呈负相关。     

两种一次性使用输液袋的致突变作用研究

【目的】对一次性使用聚丙烯输液袋和聚氯乙烯输液袋的致突变作用进行初步评价。【方法】应用Ames试验和小鼠骨髓细胞微核试验,研究两种输液袋的致突变作用。【结果】在Ames试验的TA97、TA98、TA100和TA102四个标准菌株±S9mix条件下,两种输液袋在0.5~500.0μg/皿浓度范围内均未诱发菌株回变菌落数明显增加。5.0 mg/kg、10.0 mg/kg和20.0 mg/kg各剂量组,小鼠骨髓嗜多染红细胞微核率与溶剂对照组相比未见明显增加(P>0.05)。【结论】该试验条件下,一次性使用聚丙烯输液袋和聚氯乙烯输液袋无致突变作用。     

抗枯宁的致突变性和致畸性研究

本文报道抗枯宁的致突变性和致畸性实验结果。结果表明,抗枯宁在Ames试验中,加和不加S_(99)A_(98)和A_(100)菌株回变菌落数均未超过自然回变菌落数的2倍,小鼠骨髓嗜多染红细胞微核试验和精子畸形试验结果阴性;抗枯宁没有致畸胎作用。     

除草剂丁草胺的Ames试验

用Ames试验测定了丁草胺的致变性。以鼠伤寒沙门氏菌株 TA97TA98TA100作为试测菌株,α—氨基芴为阳性诱变剂。结果不同浓度的丁草胺乳剂并未使三种菌株的回复突变率升高,丁草胺的 Ames 试验为阴性,表明丁草胺无明显的致突变性。     

"冻干江浙蛇毒"的致突变作用研究

目的:检测"冻干江浙蛇毒"的致突变性,以提供有关致突变的遗传毒性安全评价数据.方法:采用小数骨髓微核试验、微生物回复突变试验(Ames)、仓鼠肺成纤维细胞染色体畸变试验(CHL),研究"冻干江浙蛇毒"的致突变作用.结果:小鼠骨髓微核试验表明,"冻干江浙蛇毒"三个剂量组(1.0、0.5、0.25mg/kg腹腔注射)的微核出现率与阴性对照组(0.9%NaCl)比较无显著性(P＞0.05),与阳性对照组比较有显著性(P＜0.01);Ames试验表明,"冻干江浙蛇毒"采用5000、2500、1250、625和312.5ug/皿剂量时,在加和不加S9条件下,对TA97、TA98、TA100和TA102菌株回复突变菌落数结果为阴性;CHL试验也表明,"冻干江浙蛇毒"4.0、2.0、1.0和0.5ug/ml剂量对中国仓鼠肺成纤维细胞(CHL)体外培养染色体无畸变作用.结论:"冻干江浙蛇毒"无致突变性,有良好的应用前景.     

水源水及自来水致突变性研究

本研究对南宁市某自来水厂水源水及自来水用XAD-2型树脂浓集水中有机物,进行Ames试验(选用TA_(98)和TA_(100)菌株加与不加S_9),观察其致突变活性作用。结果表明,水源水和自来水有机物均引起TA_(98)和TA_(100)菌株致突变作用阳性,且有剂量与反应关系,其回变菌落数随剂量递增而增加。     

Ames试验敏感性的改进:检试菌旋转培养5小时与16小时的比较

对数期生长的细菌代谢较旺盛,其DNA在复制过程中易受致突变物的影响。据此,本文比较了旋转培养(60转/分)5小时和16小时Ames检试菌TA1535、TA100、TA98、TA37,分别对叠氮钠、苦酮酸、硝基腐植酸的致突变效应。经多次实验结果表明:旋转培养5小时的细菌受相应的致突变物诱发的回变菌落数/平板,与旋转培养16小时者相比,均有显著增加,其回变菌落数的差异经统计学处理,大都具有显著或高度显著意义。即旋转培养5小时的细菌对致突变物的敏感性有所增强。从检试菌的活菌数和自发回变菌落数来看,培养时间也不宜过长。     

包头市黄河水与自来水有机提取物致突变性研究

目的:了解黄河水源水、自来水中有机提取物的致突变性.方法:采集黄河包头段中游画匠营水源水及自来水各100L提取有机物,采用平板掺入法制成0.125、0.25、0.5、1、2(L/皿)5个浓度组.测试菌株为TA98、TA100,同时做加与不加S9(-S9、 S9)的比较.以DMSO为阴性对照,叠氮化钠、正定霉素和2,7-二胺基芴为阳性对照,计数回变菌落数.结果:自来水0.25(L/皿)-S9、 S9的回变菌落数分别是88.3±2.5和90.3±2.5,均是阴性对照组的2倍以上,随有机提取物浓度的升高回变菌落数增加,有明显的剂量反应关系.水源水出现阳性的最低剂量是1(L/皿)-S9、 S9的回变菌落数分别是76.6±13.8和87.3±5.9.结论:包头市黄河水及自来水均具有潜在的致突变活性,在相同剂量水平下自来水的致突变性强于水源水.     

不同水文期水中非挥发性有机污染物致突变性的比较

应用大孔树脂吸附浓集水中有机物的技术,结合Ames致突变试验,研究了不同水文期的东湖与汉江水源水及其所制成的自来水中非挥发性有机物的致突变性.结果发现在加与不加体外代谢活化系统(S9)的条件下,除枯水期外,其他水文时期东湖与汉江水源水中非挥发性有机物对TA98菌株的致突变试验为阳性结果;各水文期东湖与汉江自来水中有机物对TA98和TA100菌株均具有致突变性,且致突变比活性强度均明显高于水源水,各期水样致突变比活性强度由强至弱依次为平水期＞枯水期＞丰水期.     

猴头多糖(HEPS)遗传毒性评价及抗突变实验研究

目的与方法　采用经典Ames试验评价猴头多糖 (HEPS)的遗传毒性及抗突变作用。结果　HEPS 3 0mg/皿、15mg/皿、7 5mg/皿、3 75mg/皿、1 875mg/皿 5个剂量组在 +S9和 -S9时 ,均未能引起TA 98和TA 10 0两测试菌株回变菌落数增加 ,亦未观察到剂量反应关系。本次研究HEPS 2 0mg/皿、10mg/皿、5mg/皿三个剂量组都可显著抑制阳性物敌克松和叠氮钠引起的TA 10 0回变菌落数增加 (P <0 0 5 )。结论　本实验条件下HEPS致突变作用阴性 ,HEPS具有抗突变作用。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…