Clinical Observation of Bronchial AsthmaTreeted with Qingre Dingchuan Decoction（清热定喘汤）

ＣｌｉｎｉｃａｌＯｂｓｅｒｖａｔｉｏｎｏｆＢｒｏｎｃｈｉａｌＡｓｔｈｍａＴｒｅｅｔｅｄｗｉｔｈＱｉｎｇｒｅＤｉｎｇｃｈｕａｎＤｅｃｏｃｔｉｏｎ（清热定喘汤）ＣｈｕＤｏｎｇ－ｎｉｎｇ（褚东宁）（Ｐｅｏｐｌｅ＇ｓＨｏｓｐｉｔａｌｏｆＳｈａｎｇｃｈｅｎｇＤ...     

肺良恶性疾病患者血LPO、SOD、GSH-Px测定及临床意义

测定了10例健康人、20例肺良性疾病及15例肺癌患者血LPO含量、GSH-Px活性、SOD活性及SOD/LP0比值,结果①肺癌组SOD活性,SOD/LPO比值均显著低于正常组及良性组(P<0.01;<0.05;<0.01;<0.01);②正常组及良性组SOD活性及SOD/LPO比值差别无显著性(分别P>0.05);③LPO含量及GSH-Px活性三组之间差别无显著性(分别P>0.05);④若将正常组SOD及SOD/LPO比值的均数分别减去一个标准差(x-s)作为肺癌的诊断试验阳性标准,其灵敏度分别为73.3％和73.3％,特异度为80％和70％;诊断符合率为77.1％和71.4％。上述结果提示肺癌的发生发展可能与氧自由基损伤有关。通过SOD活性及SOD/LPO比值的测定可作为鉴别肺部良恶性疾病的辅助诊断手段之一,对肺癌的辅助诊断也有一定价值。     

血液中氧自由基浓度与突发性耳聋的关系及其临床意义

目的 :探讨血液中氧自由基浓度与突发性耳聋的关系及其临床意义。方法 :检测了 93例突发性耳聋患者与 10 0例正常健康者血浆中的超氧化歧化酶 ( P- SOD)活性和过氧化脂质 ( P- L PO)含量 ,以及红细胞中的超氧化物歧化酶 ( E- SOD)活性和过氧化脂质 ( E- L PO)含量 ,并作分析比较。结果 :与对照组比较 ,患者组的 P- SOD、E- SOD平均活性显著降低 ( P<0 .0 0 1) ,而 P- LPO、E- LPO平均含量显著增高 ( P<0 .0 0 1) ;与前庭功能正常组相比较 ,前庭功能异常组的 P- SOD、E- SOD平均值均显著降低 ( P<0 .0 0 0 1) ,P- LPO、E- LPO平均值均显著升高 ( P<0 .0 0 0 1) ;患者的听阈 ( HT)值随着 P- SOD和 E- SOD活性的下降及 P- LPO和 E- LPO含量的增高而增高 ;并均呈直线相关 ( P<0 .0 0 0 1) ;患者治疗后的 P- SOD、E- SOD平均值均显著升高 ( P<0 .0 0 0 1) ,P- L PO、E- L PO、HT平均值均显著降低 ( P<0 .0 0 0 1)。结论 :突发性耳聋患者体内的自由基反应病理性加剧 ,氧化抗氧化动态平衡严重失调。     

口腔扁平苔藓在中西药物治疗前后血清SOD和LPO水平的变化

目的　探讨中西药物治疗对口腔扁平苔藓 (OLP)患者血清超氧化物歧化酶 (SOD)和脂质过氧化物 (LPO)水平变化的关系。方法　应用中西药物治疗OLP一疗程 ,观察其疗效 ,并测定治疗前后血清SOD和LPO水平的变化。结果　临床总有效率为 76.19% ,痊愈率为 3 8.10 %。治疗前血清SOD和LPO与正常对照组比较 ,差异有显著性 (P <0 .0 0 1) ;治疗后SOD水平显著升高 ,LOP水平下降明显 ,与正常对照组比较 ,差异无显著性 (P >0 .0 5 )。结论　中西药物治疗OLP临床疗效较好 ,可能与提高SOD水平 ,降低LPO水平有关。     

丹参对视网膜静脉阻塞患者脂质过氧化物及超氧化物歧化酶的影响

目的：探讨丹参对视网膜静脉阻塞（ＲＶＯ）患者血浆脂质过氧化物（ＬＰＯ）含量及红细胞超氧化物歧化酶（ＳＯＤ）活性的影响。方法：静脉滴注丹参注射液治疗３６例ＲＶＯ患者,测定治疗前后ＲＶＯ患者ＬＰＯ含量及ＳＯＤ活性,选取３０例健康人作为对照。结果：与健康人相比,ＲＶＯ患者血浆ＬＰＯ含量明显升高,红细胞ＳＯＤ活性明显下降（Ｐ＜０．００１）;ＲＶＯ患者使用丹参治疗后血浆ＬＰＯ含量较治疗前下降（Ｐ＜０．０５）,红细胞ＳＯＤ活性较治疗前明显升高（Ｐ＜０．０１）;ＲＶＯ患者使用丹参治疗后血浆ＬＰＯ含量及红细胞ＳＯＤ活性与健康人相比,均无明显变化（Ｐ＞０．０５）。结论：丹参作为一种外源性自由基清除剂,可有效地增加ＲＶＯ患者红细胞ＳＯＤ活性,降低ＬＰＯ含量,对ＲＶＯ的防治有重要意义     

Effect of puerarin on fibrinolytic activity and lipid peroxide in patients with coronary heart disease

Effect of Puerarin on Fibrinolytic Activity and Lipid Peroxide in Patients with Coronary Heart Disease@陈江斌 @许家俐 @李建军 @黄从新 @唐其柱 @王晋明 @李庚山     

Abnormal metabolism of nitric oxide, oxidative stress and lipoperoxidative stress in patients with acute viral myocarditis

Objective To investigate metabolic status of nitric oxide (NO) as well as oxidative and li poperoxidative stress, pathological chain reactions of a series of free radicals , and oxidative and lipoperoxidative damages in patients with acute viral myocar ditis (AVM). Methods Using a random paired control design, plasma levels of NO, lipoperoxides (LPO), vitamin C (VC), vitamin E (VE) and β- carotene (β- CAR), erythrocytic level of LPO as well as activities of erythrocytic superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH- Px) were investigated by spectrophotometr ic assays in 60 AVM patients and 60 healthy adult volunteers (HAV). Results Compared with the HAV group, plasma NO, and LPO in plasma and in erythrocyte of the AVM group significantly increased (P=0. 0001), while VC, VE, β- CAR, SO D, CAT and GSH- Px of the AVM group significantly decreased (P=0. 0001). Co mpared with the AVM group before treatment, plasma NO, and LPO in plasma and in erythrocyte of the AVM group post treatment significantly decreased (P&lt;0. 05 ), whereas plasma VC, VE and β- CAR as well as erythrocytic SOD, CAT and GSH- P x of the AVM group post treatment significantly increased (P&lt;0. 05). Conclusion The findings in this study suggested that in the AVM patients the metabolism of NO was disturbed, and the pathological chain reactions of a series of free radic als were severely aggravated, thus produced the oxidative damage and lipoperoxid ative damages. Therefore, we recommend that antioxidants at suitable dosage, su ch as VC, VE, β- CAR and others, should be given to AVM patients daily to allev iate potential oxidative and lipoperoxidative damages in their bodies.     

1，6－二磷酸果糖对病毒性心肌炎患儿脂质过氧化物的影响

本文观察了１，６－二磷酸果糖（ＦＤＰ）对病毒性心肌炎患儿血中脂质过氧化物（ＬＰＯ）、超氧化物歧化酶（ＳＯＤ）的影响。结果显示病毒性心肌炎患儿血中ＬＰＯ高于正常对照组，ＳＯＤ则低于正常对照组。与１０％葡萄糖静点组对照ＦＤＰ治疗可显著降低ＬＰＯ水平，但不伴有ＳＯＤ的明显改变，提示ＦＤＰ是保护心肌的有效抗氧化剂。     

各型乙肝患者过氧化脂质和超氧化物岐化酶含量

检测54例乙肝患者血清LPO和红细胞SOD含量,并进行了动态观察。探讨了乙肝病人血清LPO与红细胞SOD水平的关系,LPO和SOD与ALT的关系。结果:三组乙肝血清LPO水平明显升高(P<0.01);慢重型乙肝LPO水平比急性和慢活型乙肝明显升高(P<0.01)。当ALT>200~u时LPO水平升高(P<0.05),<30~u时血清LPO水平差异不明显(P>0.05)。急性乙肝LPO上升,而红细胞SOD明显下降(P<0.01)。慢活型、慢重型乙肝比急性乙肝SOD水平升高、明显升高(P<0.05,P<0.01)。当ALT>200~u时红细胞SOD水平明显降低(P<0.01),200—30~u和<30~u者SOD水平显著升高(P<0.01)。乙肝恢复期(ALT<30~u时)血清LPO水平与正常人无差异(P>0.05),其中7例慢重型乙肝病情恶化时LPO水平持续高水平。乙肝病人红细胞SOD水平随病情的好转而上升(P<0.01)。结果表明,乙肝患者LPO水平高低可反映肝功能受损程度,特别是重型乙肝更为明显。而乙肝红细胞SOD水平升高和降低的论点待进一步验证。     

Relationship between Acute Organophosphours Pesticide Poisoning and Damages Induced by Free Radicals

Objective To study the relationship between abnormal reactions of free radicals in bodies of patients with acute organophosphorus pesticide poisoning (AOPP) and damages induced by free radicals. Methods 58 AOPP patients and 58 healthy adult volunteers (HAV) were enrolled in an independent samples control design, in which spectrophotometric methods were used to determine the concentrations of nitric oxide (NO) and lipoperoxides (LPO) in plasma, and LPO in erythrocytes, vitamin C (VC), vitamin E (VE) and b-carotene (b-CAR) in plasma as well as activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and acetylcholinesterase (AChE) in erythrocytes. Results Compared with the average values of every biochemical parameter in the HAV group, the average values of LPO in plasma and in erythrocytes, and NO in plasma in the AOPP group were significantly increased (P=0.000001), while the average values of VC, VE, a-CAR in plasma as well as SOD, CAT, GSH-Px and AChE in erythrocytes in the AOPP group were significantly decreased (P=0.000001). The findings of Pearson product-moment correlation analysis between the value of AChE in erythrocytes and the values of above biochemical parameters for 58 AOPP patients showed that there was a significant linear negative correlation between AChE in erythrocytes and LPO, NO in plasma, and LPO in erythrocytes (P=0.000001～0.001319), while there was a significant linear positive correlation between AChE in erythrocytes and VC, VE, a-CAR in plasma as well as SOD, CAT, GSH-Px in erythrocytes (P=0.000013～0.000824). The results of discriminant analysis of above chemical parameters for 58 AOPP patients and 58 HAV suggested that the correct rates of discriminant analysis were increased to 100 % when the values of AChE and LPO in plasma and in erythrocytes, or AChE and others, were jointly used for the discriminant analysis. Conclusion The findings of the present study suggest that a series of free radical reactions in AOPP patients' bodies are pathologically aggravated, and the discriminant analysis used the above biochemical parameters could markedly increase its correct rates for AOPP patients.     

老年充血性心力衰竭与氧自由基、脑钠素的关系及川芎嗪干预效果研究

目的观察川芎嗪治疗老年充血性心力衰竭（CHF）的效果及对氧自由基（OFR）和脑钠素（BNP）的影响。方法选择45例正常对照组及90例老年CHF患者,并随机分为常规治疗组和川芎嗪组,两组共治疗4周,对照组及两组患者治疗前后进行血清超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）、过氧化脂质（LPO）及BNP水平进行测定,并观察两组CHF病人治疗前后NYHA分级及心功能指标的变化。结果常规治疗组、川芎嗪组治疗前SOD,CAT水平明显低于正常对照组（P〈0．01）;MDA,LPO,BNP明显高于正常对照组（P〈0．01）;川芎嗪组治疗后SOD,CAT水平明显高于治疗前及常规治疗组（P〈0．01）;MDA,LPO,BNP明显低于治疗前及常规治疗组（P〈0．01）;两组CHF病人治疗后NYHA分级及心功能指标均得到显著改善（P〈0．01）,但川芎嗪组较常规治疗组改善更明显（P〈0．01）。结论川芎嗪有明显的抗氧化作用,并能降低BNP水平,从而改善CHF患者的心功能,血浆BNP,SOD,CAT,MDA,LPO浓度为心力衰竭严重程度、判断疗效的敏感指标,值得临床推广和应用。     

Relationship between Acute Organophosphorus Pesticide Poisoning and Damages Induced by Free Radicals

~~ Relationship between Acute Organophosphorus Pesticide Poisoning and Damages Induced by Free Radicals~~     

川芎嗪对黄褐斑患者血脂过氧化物、超氧化物歧化酶含量的影响

目的：研究川芎嗪对黄褐斑患者血脂过氧化物（LPO）、超氧化物歧化酶（SOL）含量的影响。方法：将60例黄褐斑患者随机分为两组，治疗组32例，对照组28例。治疗组每天静脉滴注川芎嗪160mg,疗程15天，测定治疗组静脉滴注川芎嗪前后LPO、SOD的含量变化，对照组直接从静脉取血测定LPO、SOD的含量。结果：川芎嗪治疗后，黄褐斑患者LPO含量明显降低，SOD含量明显升高，黄褐斑症状也明显改善。结论：川芎嗪能明显降低黄褐斑患者LPO含量，升高SOD含量，对治疗黄褐斑有较高的临床实用价值。     

康复新液联合双歧杆菌三联活菌胶囊对溃疡性结肠炎患者血清LPO、SOD水平及生活质量的影响

目的 探讨康复新液联合双歧杆菌三联活菌胶囊对溃疡性结肠炎患者血清脂质过氧化物（LPO）、超氧化物歧化酶（SOD）水平及生活质量的影响。方法 选取2015年1月—2017年3月河北省中医院129例溃疡性结肠炎患者,依据随机双盲法分为研究组、对照A组及对照B组,各43例。对照A组予以康复新液治疗,对照B组予以双歧杆菌三联活菌胶囊治疗,研究组予以康复新液联合双歧杆菌三联活菌胶囊治疗。观察统计3组治疗效果、药物不良反应,并对治疗前后3组血清LPO、SOD水平及生活质量变化情况进行比较。结果 研究组治疗总有效率与对照A、B组比较,差异有统计学意义（P?<0.05）,研究组高于对照A、B组。治疗后,3组血清LPO、SOD水平、生活质量评分比较,差异有统计学意义（P?<0.05）;研究组血清LPO水平低于对照A、B组。血清SOD水平及生活质量评分均高于对照A、B组;治疗后,研究组血清LPO、SOD水平较治疗前均下降,生活质量评分均升高（P?<0.05）;对照A、B两组血清LPO、SOD水平、生活质量评分比较,差异无统计学意义（P?>0.05）。3组治疗期间药物不良反应发生率比较,差异无统计学意义（P?>0.05）。结论 对溃疡性结肠炎患者采用康复新液联合双歧杆菌三联活菌胶囊治疗,可有效降低血清LPO水平,提高血清SOD水平,改善患者生活质量,提高治疗效果,且安全性较高。     

促红细胞生成素对脑创伤患者外周血LPO、SOD的影响

目的观察给予促红细胞生成素(EPO)对脑创伤(TBI)患者外周血中脂质过氧化物酶(LPO)、内源性过氧化物歧化酶(SOD)的影响。方法将70例TBI患者随机分成2组,即治疗组和病理对照组,入院后均进行CT检查、GCS评分,根据GCS评分将各组又分成轻、中、重3组。所有患者均按照常规进行治疗,治疗组患者在常规治疗基础上加用皮下注射EPO,并于伤后24h、治疗3周后即刻及治疗后1个月、3个月抽取外周血进行SOD及LPO水平的检测,于治疗后1个月进行格拉斯格预后评分(GOS)。选择来院体检的正常人群为对照组。结果给予EPO治疗后的中度TBI患者在治疗后即刻、1个月及3个月外周血中SOD及LPO水平与病理对照组比较差异均有统计学意义(P<0.05),治疗后1个月的GOS评分与病理对照组比较亦差异显著(P<0.05);轻度及重度患者在上述时间点上SOD、LPO水平及治疗后1个月的GOS评分与病理对照组比较差异不明显。入院初病情等级与SOD/LPO比值呈负相关,而治疗组治疗后1个月GOS评分与SOD/LPO比值呈正相关。结论TBI后SOD/LPO平衡受到破坏,及时给予EPO治疗,可以恢复SOD/LPO平衡,改善预后。     

调平康治疗Ⅰ─Ⅱ期高血压病作用机理探讨

采用单盲、随机对照试验，将５９例主要为肝肾阴虚兼阳亢证的Ⅰ─Ⅱ期高血压病患者分为中药组、西药组、中西药组，分别给予调平康、心痛定及两药合用进行治疗。同时观察治疗前后上述病人及２０例正常对照者血液的循环内皮细胞计数、过氧化脂质、前列环素和超氧化物歧化酶值。结果表明：高血压病患者存在脂质过氧化反应增强和血管内皮细胞结构和功能的损伤。而调平康能降低过氧化脂质值和循环内皮细胞计数值，同时升高前列环素值和超氧化物歧化酶活性。提示：增强机体抗氧化能力、抑制脂质过氧化反应、减轻血管内皮细胞损伤，可能是滋补肝肾之调平康治疗高血压病的重要机制之一。     

Oxidative Stress in Patients With Acute Coxsackie Virus Myocarditis

To study the state of oxidative stress in patients with acute coxsackie virus myocarditis (ACM), and to investigate the pathological chain reactions of a series of free radicals and oxidative and lipoperoxidative damages in their bodies. Methods Eighty ACM patients and 80 healthy adult volunteers (HAV) were enrolled in a case-control study, in which concentrations of nitric oxide (NO) in plasma, lipoperoxides (LPO) in plasma and LPO in erythrocytes (RBC), vitamin C (VC), vitamin E (VE) and β-carotene (β-CAR) in plasma as well as activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in RBC were determined by using spectrophotometric assays. Results Compared with the average values (AV) of the above biochemical parameters (BP) in the HAV group, the AV of NO in plasma, and LPO in plasma and RBC in the ACM group were significantly increased (P=0.0001), while the AV of VC, VE, β-CAR, SOD, CAT and GSH-Px in the ACM group were significantly decreased (P=0.0001). The values of the above BP were used to estimate the relative risk ratio (RR) between the ACM group and the HAY group; the RR and its 95 % confidence interval were 12.467 (5.745～27.051), 4.333(2.126～8.834), 6.517 (3.225～13.618), 3.310 (1.598～6.858), 31.000 (12.611～76.201),4.663 (2.228～9.759), 11.769 (5.440～25.462), 3.043 (1.486～6.229) and 6.594 (3.045～14.281)respectively, and their P levels ranged from 0.002 to 0.0001. The results were asfollows: D = 22.143 - 0.017SOD + 0.008NO + 0.244LPO in RBC, Eigenvalue = 13.659,Canonical correlation = 0.965, Wilks' λ = 0.068, χ2 = 420.212, P = 0.0001. The correct rate of discrimination to the ACM group and to the HAV group was 87.5% and 95.0 %, respectively,and 91.3 % of originally grouped cases was correctly classified. Conclusion The findings in this study suggested that the oxidative stress in bodies of ACM patients was severely aggravated, and marked high oxidative constituents and low antioxidants and antioxidases in the human body might increase the relative risk of inducing acute coxsackie virus myocarditis, and measuring the values of NO in plasma, SOD and LPO in RBC might increase the correct rates of discriminatory analysis of the ACM.     

急性脑血管病患者血浆过氧化脂质和红细胞超氧化物歧化酶含量观察

测定６９例脑血管病急性期患者血浆过氧化脂质（ＬＰＯ）和红细胞超氧化物歧化酶（ＳＯＤ）的活性并与健康对照组比较，结果：脑出血与脑梗塞患者的ＬＰＯ明显增高，而ＳＯＤ明显降低。在脑梗塞患者ＬＰＯ增高与ＳＯＤ降低呈负相关，而脑出血者无此类负相关。     

辛伐他汀与灯盏花素联合治疗对冠心病患者血液流变学及LPO、SOD的影响

目的：研究辛伐他汀与灯盏花素联合治疗对冠心病（ CHD）患者血液流变学指标、血清脂质过氧化物（ LPO）及超氧化物歧化酶（ SOD）的影响。方法将140例CHD患者随机分成观察组和对照组,每组各70例,在治疗前与治疗后的第14 d检测患者血液流变学指标、LPO及SOD水平变化。结果观察组总有效率为97.1％。对照组为75.7％,两组疗效比较,差异有统计学意义（ P＜0.05）;观察组血液流变学指标较治疗前明显改善,接近正常水平,对照组血液流变学亦有改善,但不如观察组明显;观察组LPO明显下降、SOD明显升高,治疗前后比较,差异有统计学意义（ P＜0.05）,对照组无明显改善。结论辛伐他汀与灯盏花素联合治疗可明显改善CHD患者的血液流变学指标,提高SOD水平,降低LPO水平,临床治疗效果较好。     

髙压氧联合血栓通治疗对脑梗死患者血液流变学及LPO、SOD的影响

目的：研究高压氧联合血栓通治疗对脑梗死患者血液流变学及血清脂质过氧化物（LPO）、超氧化物歧化酶（SOD）的影响。方法：将脑梗死患者120例随机平分成高压氧组（高压氧加血栓通治疗）和对照组（血栓通治疗）,治疗前及治疗后14 d检测血液流变学指标及LPO、SOD水平变化。结果：高压氧治疗组总有效率为91.7%,对照组为71.7%,两组疗效比较,差异有统计学意义（P〈0.01）;高压氧组血液流变学指标较治疗前明显改善,接近正常水平,对照组血液流变学亦有改善,但不如高压氧组明显;高压氧组LPO明显下降、SOD明显升高,治疗前后比较,差异有统计学意义（P〈0.01）,而对照组无明显改善。结论：高压氧联合血栓通治疗可明显改善脑梗死患者的血液流变学指标、提高SOD水平、降低LPO水平,并获得较好的临床治疗效果。