共查询到20条相似文献，搜索用时 31 毫秒
1.
ˎ̥,Verdana,Arial'; mso-hansi-font-family: 'ˎ̥,Verdana,Arial'; mso-bidi-font-family: 宋体">目的 制备抗泡球蚴组织单克隆抗体,并鉴定其特异性。 方法 运用泡球蚴组织粗抗原免疫小鼠制备免疫脾细胞,运用淋巴细胞杂交瘤技术将其与小鼠骨髓细胞SP2/0融合。采用ELISA和免疫组化方法筛选抗泡球蚴原头节单克隆抗体的杂交瘤细胞株。检测所获细胞株的染色体数目,采用ELISA和免疫组化法鉴定所获抗体的特异性。 结果 获得了一株能稳定分泌的抗泡球蚴原头节单克隆抗体的杂交瘤细胞株6G2A7F7。染色体数目为98条,为明显的融合细胞核型。所分泌的抗体McAb P325能与泡球蚴中的生发层和原头节特异结合,特别对原头节上的小钩和吸盘表现出很强的结合反应。但与棘球蚴（人源）和水泡带绦虫幼虫（细颈囊尾蚴）组织无特异性结合反应。 结论 制备的杂交瘤细胞能稳定分泌McAb P325,为泡球蚴的细胞分类、免疫组化和抗原研究提供工具和基础。 相似文献
2.
目的：比较不同时期采用经皮胃镜下空肠造瘘术进行肠内营养的重症脑出血患者腹泻发生率。方法：回顾性分析63例采用经皮胃镜下空肠造瘘术进行肠内营养的重症脑出血患者,按经皮胃镜下空肠造瘘术距离患者发病时间分组,早期组(3天以内)32例,晚期组(3天以后)31例,比较两组患者腹泻发生率。 结果：早期组患者腹泻发生率为12.5%,晚期组患者腹泻发生率为35.5%,早期组患者腹泻发生率明显低于晚期组患者(P值<0.05)。 结论：重症脑出血患者早期采用经皮胃镜下空肠造瘘术肠内营养可降低腹泻发生率。 相似文献
3.
Protective effect of Weikang decoction and partial ingredients on model rat with gastric mucosa ulcer 总被引：1，自引：0，他引：1
Fan TY Feng QQ Jia CR Fan Q Li CA Bai XL 《World journal of gastroenterology : WJG》2005,11(8):1204-1209
AIM, To investigate the protective mechanisms of Weikang (WK) decoction on gastric mucosae. METHODS: Ninety rats were randomly divided into nine groups of 10 each, namely group, model group, group with large WK dosage, group with medium WK dosage, group with small WK dosage, group with herbs of jianpiyiqi (strengthening the spleen and replenishing qi), group with herbs of yangxuehuoxue (invigorating the circulation of and nourishing the blood), group with herbs of qingrejiedu (clearing away the heat-evils and toxic materials), group with colloidal bismuth pectin (CBP) capsules. According to the method adopted by Yang Xuesong, except normal control group, chronic gastric ulcer was induced with 100% acetic acid. On the sixth day after moldmaking, WK decoction was administered, respectively at doses of 20, 10 and 5 g/kg to rats of the WK groups, or the group swith herbs of jianpiyiqi, yangxuehuoxue and qingrejiedu, 10 mL/kg was separately administered to each group every day. For the group with CBP capsules, medicine was dissolved with water and doses 15 times of human therapeutic dose were administered (10 mL/kg solution containing 0.35% CBP). Rats of other groups were fed with physiological saline (10 mL/kg every day) .Administration lasted for 16 d. Rats were killed on d 22 after mold making to observe changes of gastric mucosa. The mucus thickness of gastric mucosa surface was measured. Levels of epidermal growth factor (EGF) in gastric juice, nitric oxide (NO) in gastric tissue, endothelin (ET) in plasma, superoxide dismutase (SOD) in plasma, malondialdehyde (MDA) in plasma and prostaglandin I2 (PGI2) were examined. RESULTS: Compared with control group, ulceration was found in gastric mucosa of model group rats. The mucus thickness of gastric mucosa surface, the levels of EGF, NO, 6-K-PGF1α and SOD decreased significantly in the model group (EGF: 0.818±0.18 vs 2.168±0.375, NO: 0.213±0.049 vs0.601±0.081, 6-K-PGF1α: 59.7±6.3 vs 96.6±8.30, SOD: 128.6±15.0 vs 196.6±35.3, P<0.01), the levels of ET (179.96±37.40 vs 46.64±21.20, P<0.01) and MDA (48.2±4.5 vs 15.7±4.8, P<0.01) increased. Compared with model group, the thickness of regenerative mucosa increased, glandular arrangement was in order, and cystic dilative glands decreased, while the mucus thickness of gastric mucosa surface increased (20 g/kg WK: 51.3±2.9 vs 23.2±8.4, 10 g/kg WK: 43.3±2.9 vs 23.2±8.4, 5 g/kg WK：36.1±7.2 vs 23.2±8.4, jianpiyiqi: 35.4±5.6 vs 23.2±8.4, yangxuehuoxue: 33.1±8.9 vs 23.2±8.4, qingrejiedu: 31.0±8.0 vs 23.2±8.4 and CBP: 38.2±3.5 vs 23.2±8.4, P<0.05-0.01). The levels of EGF (20 g/kg WK: 1.364±0.12 vs 0.818±0.18, 10 g/kg WK: 1.359±0.24 vs 0.818±0.18, 5 g/kg WK: 1.245±0.31 vs 0.818±0.18, jianpiyiqi： 1.025±0.45 vs 0.818±0.18, yangxuehuoxue: 1.03±0.29 vs 0.818±0.18, qingrejiedu: 1.02±0.47 vs 0.818±0.18 and CBP:1.237±0.20 vs 0.818±0.18, P<0.05-0.01), NO (20 g/kg WK: 0.480±0.026 vs 0.213±0.049, 10 g/kg WK: 0.390±0.055 vs 0.213±0.049, 5 g/kg WK: 0.394±0.026 vs 0.213±0.049, jianpiyiqi: 0.393±0.123 vs 0.213±0.049, yangxuehuoxue: 0.463±0.077 vs 0.213±0.049, qingrejiedu: 0.382±0.082 vs 0.213±0.049 and CBP： 0.395±0.053 vs 0.213±0.049, P<0.05-0.01), 6-K-PGF1α (20 g/kg WK: 86.8±7.6 vs 59.7±6.3, 10 g/kg WK: 77.9±7.0 vs 59.7±6.3, 5 g/kg WK:70.0±5.4 vs 59.7±6.3, jianpiyiqi: 73.5±12.2 vs 59.7±6.3, yangxuehuoxue: 65.1±5.3 vs 59.7±6.3, qingrejiedu: 76.9±14.6 vs 59.7±6.3, and CBP: 93.7±10.7 vs 59.7±6.3, P<0.05-0.01) and SOD (20 g/kg WK: 186.4±19.9 vs 128.6±15.0, 10 g/kg WK: 168.2±21.7 vs 128.6+15.0, 5 g/kg WK: 155.6±21.6 vs 128.6±15.0, jianpiyiqi: 168.0±85.3 vs 128.6±15.0, yangxuehuoxue: 165.0±34.0 vs 128.6±15.0, qingrejiedu: 168.2±24.9 vs 128.6±15.0, and CBP: 156.3±18.1 vs 128.6±15.0, P<0.05-0.01) significantly increased. The levels of ET (20 g/kg WK: 81.30±17.20 vs 179.96±37.40, 10 g/kg WK: 83.40±25.90 vs 179.96±37.40, 5 g/kg WK: 93.87±20.70 vs 179.96±37.40, jianpiyiqi: 130.67±43.66 vs 179.96±37.40, yangxuehuoxue: 115.88±34.09 vs 179.96±37.40, qingrejiedu: 108.22±36.97 vs 179.96+37.40,and CBP: 91.96±19.0 vs 179.96±37.40, P<0.01) and MDA (20 g/kg WK: 21.6±7.4 vs 48.2±4.5, 10 g/kg WK: 32.2±7.3 vs 48.2±4.5, 5 g/kg WK: 34.2±6.2 vs 48.2+4.5, jianpiyiqi: 34.9±13.8 vs 48.2±4.5, yangxuehuoxue: 35.5±16.7 vs 48.2±4.5, qingrejiedu: 42.2±17.6 vs 48.2±4.5, and CBP: 30.1±6.1 vs 48.2±4.5, P<0.05-0.01) obviously decreased. The 20 g/kg WK group was better than 10 g/kg (the mucus thickness: 51.3±2.9 vs 43.3±2.9, NO: 0.480±0.026 vs 0.390±0.055, SOD: 186.4±19.9 vs 168.2±21.7, P<0.01) and 5 g/kg (the mucus thickness: 51.3±2.9 vs 36.1±7.2, NO: 0.480±0.026 vs 0.394±0.026, SOD: 186.4±19.9 vs 155.6±21.6, P<0.01) groups and CBP group (the mucus thickness: 51.3±2.9 vs 38.2±3.5, NO: 0.480±0.026 vs 0.395±0.053, SOD: 186.4±19.9 vs 156.3±18.1, P<0.01) in the mucus thickness, NO and SOD levels and better than 10 g/kg (86.8±7.6 vs 77.9±7.0, P<0.05) and 5 g/kg (86.8±7.6 vs 70.0±5.4, P<0.05) groups in 6-K-PGF1α level, 10 g/kg WK group was better than 5 g/kg WK (the mucus thickness: 43.3±2.9 vs 36.1±7.2, P<0.01, SOD: 168.2±21.7 vs 155.6±21.6, P<0.05) and CBP groups (the mucus thickness: 43.3±2.9 vs 38.2±3.5, P<0.01, SOD: 168.2±21.7 vs 156.3±18.1, P<0.05) in the mucus thickness and SOD level. In compound group, jianpiyiqi group, yangxuehuoxue group, qingrejiedu group, the level of ET was decreased, NO contents were increased in gastric tissue of ulcers in rats. CONCLUSION: WK decoction and separated recipes have significantly protective effect on ethanol-induced gastric mucosal injury. They can increase the content of EGF in gastric juice, PGI2 SOD in plasma and NO in gastric tissues, thicken the mucus on the gastric mucosa, and decrease the impairing factor MDA, ET in plasma. 相似文献
4.
AIM: To investigate the mRNA expression of gammaaminobutyric acid A (GABAA) receptor subunitsα1,β1,γ2 in different parts of the brain of rats with hepatic encephalopathy.METHODS: Twelve adult male Sprague-Dawley rats were randomly divided into two groups: (1) hepatic encephalopabhy model group (n = 6), which was induced by intraperitoneal injection of thioacetamide (TAA, 350 mg/kg) for three consecutive days; (2) control group (n = 6), in which the rats were treated with same dose of normal saline solution. After the freeze slice of cerebrum was made, in situ hybridization was used to detect the mRNA of GABAA receptor subunits α1,β1,, and γ2 in rat cerebral cortex,basal nuclei, substantia nigra and hippocampi. Image data were collected and analyzed quantitatively by QWin550CW model image signal gather and analysis system.RESULTS: In rats with hepatic encephalopathy, mRNA expression levels of GABAA receptor subunits α1,β1 increased significantly in basal nuclei, substantia nigra pars compacta, substantia nigra pars reticularis and hippocampi (144.7±15.67/184.14±4.41, 60.61±33.66/113.07±32.44,87.71± 21.25/128.40±18.85, 122.34±5.56/161.60±4.56,123.29±5.21/140.65±4.15, 123.40±4.42/140.09±4.52,124.76±4.18/140.09±4.12, 141.62±15.09/182.80±5.20,69.13±30.74/134.21±43.76, 87.87±25.16/151.01±19.49,122.14±6.30/162.33±3.92, 122.81±5.09/137.19±7.12,123.00±4.63/138.11±5.92, 125.75 ±2.43/138.81±6.10,P<0.01), but did not change in the cerebral cortex compared to the control group. Similar changes were found in the mRNA expression levels of GABAA receptor subunit γ2,which increased significantly in basal nuclei, substantia nigra pars compacta, substantia nigra pars reticularis (136.81±26.41/167.97±16.23, 51.00±36.14/113.18±36.52,86.35±20.30/126.90±19.74, P<0.01), CA1 of hippocampal(162.15:1:9.05/178.62:1:6.45, P<0.05), and no changes were found in the cerebral cortex and CA2, CA3, CA4 of hippocampi.CONCLUSION: In rats with hepatic encephalopathy,mRNA expression levels of GABAA receptor subunits α1,β1,γ2 increase significantly in basal nuclei, substantia nigra and hippocampi, suggesting that the changes of mRNA expression levels in GABAA receptor subunits may contribute to the pathogenesis of hepatic encephalopathy. 相似文献
5.
《中国心血管病研究杂志》2009,(3):233-233
第一批卫生部心律失常介入诊疗（导管消融和植入器械）培训基地名单：北京协和医院;北京协和医学院阜外心血管病医院;北京大学第一医院;北京大学人民医院;首都医科大学附属安贞医院;首都医科大学附属朝阳医院;天津医科大学总医院;天津医科大学第二医院;天津市胸科医院;河北省人民医院;山西医科大学第一医院;内蒙古自治区医院;中国医科大学第一附属医院; 相似文献
6.
Relationship between lymphocyte apoptosis and endotoxin translocation after thermal injury in rats 总被引：3，自引：0，他引：3
Xia PY Zheng J Zhou H Pan WD Qin XJ Xiao GX 《World journal of gastroenterology : WJG》2002,8(3):546-550
AIM：To investigate the relationship between lymphocyte papotosis in peripheral blood,spleen and mesenteric lymph nodes(MLN)and endotoxin translocation after thermal injury in rats.METHODS:In a wistar rat model infilicted with 30% TBSA Ⅲdegree scalding,serum LPS levels in portal vein and vena cava were quantified by tachypleus amebocyte lysate(TAL) technique.The analysis of peripheral bolld lymphocyte was employed in in situ Cell Death Detection Kit and evaluated by flow cytometry.Apoptotic lymphocytes in paraffinembedded spleen and MLN sections were examined by histologic analysis,in situ deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)and peroxidase(POD) staining.The imagines were taken by Cooldccd camera system,and the count and optical density value (transmission light) of apoptotic lymphocytes were analyzed with software Spot and lmagine proplus 4.10a(IPP4.10a).RESULTS:In the Period of 3 to 48 postburn hours(PBHs) serum LPS level(×10%3EU.L^-1)in portal vein(2.11±0.02,5.66±0.20,3.70±0.22,2.56±0.28,0.90±0.11)was higher than that in vena cava(0.63±0.01,.1.53±0.18,0.83±0.32,0.52±0.12,0.23±0.02,P<0.01),but both increased sharply in postburn rats(P<0.01)and reached a peak at 6 PBH.Analysius of apoptotic lymphocytes showed that the proportion(%) of postburn apoptotic cells was much higher than that in healthy rats(8.34±1.53,8.13±1.81,20.77±3.94,23.90±3.92,11.23±1.35and 13.26±2.09at3,6,12,24,48and 72 PBH,respectively,vs 3.99±1.72,P<0.01)especially after 6 PBH.The concentrations of lymphocytic apoptosis at 12 and 24 PBH were markedly higher than that at other time points.Meantime,few apoptotic lymphocytes were found in normal MLN,but increased postburn obviously(3±1vs 546±83,285±39,149±30,58±10,36±11and 33±9in turn,P<0.01),especially at 3 PBH,whereas apoptotic lymphocytes were concentrated in splenic cortex before the burn and decreased obviously during 72PBHs(499±186vs12±8,19±15,12±7,100±15,123±25and 226±26in turn,P<0.01) though a slight rise was found in the medulla after 24 PHB Optical density of apoptotic lymplhocytes was significantly reduced in spleen in the 24 PBHs and raised in MLN during 48 PBHs than that prior to the burn,respectively.CONCLUSION:Gut-origin LPS is a major cause of endotoxemia taken place early in rate following severe thermal injury and could induce extensive lymphocyts apoptosis in blood and MLN,which suggests an immunosuppression state could follow the initial injury and favores a septic state based on apoptotic mechanism. 相似文献
7.
目的 探讨前列腺六次跨膜蛋白2(STAMP2)基因功能区变异与新疆维吾尔族人群代谢指标及TNFα的关系。方法 采用以流行病学调查为基础的病例研究,选取维吾尔族个体894例,以TNFα水平分组:高水平组(TNFα≥7.95 μg/L)313例,中水平组(TNFα>5.34~<7.95 μg/L)268例,低水平组(TNFα≤5.34 μg/L)313例。首先在小样本代谢综合征患者中测序筛查STAMP2基因的变异位点,采用TaqMan-PCR方法在大样本人群中进行基因型鉴定及关联研究。结果 在STAMP2基因功能区选取3个代表性变异位点(rs8122、rs1981529和rs34741656),rs8122和 rs1981529的基因型及等位基因分布在不同TNFα水平3组间差异有统计学意义(P<0.05),rs34741656的基因型及等位基因分布在3组间差异无统计学意义(P>0.05)。方差分析发现rs1981529多态AA基因型与AG基因型间的TNFα水平差异有统计学意义[(2.77±0.33) μg/L比(2.83±0.31) μg/L,P<0.05], STAMP2不同基因型间的TC、HDL-C、LDL-C、TG水平差异无统计学意义(P>0.05)。结论STAMP2基因变异与新疆维吾尔族人群TNFα相关。
相似文献
8.
目的 探讨老年糖尿病患者微血管并发症的患病率,并分析发生微血管病变的危险因素。方法 回顾性分析北京协和医院2020年1月至2022年12月收治的495例老年糖尿病患者的临床资料,收集患者基线资料和生化指标,统计糖尿病视网膜病变、糖尿病肾病、周围神经病变等微血管并发症的患病率。根据微血管并发症的类型将患者分为4组:糖尿病视网膜病变组(n=107)、糖尿病肾病组(n=81)、周围神经病变组(n=169)及非并发症组(n=138)。采用SPSS 22.0统计软件进行数据分析。根据数据类型,分别采用方差分析或χ2检验进行组间比较。采用多因素logistic回归分析糖尿病微血管病变发生的危险因素。结果 495例老年糖尿病患者中共357例发生微血管并发症,总患病率为72.12%,其中糖尿病视网膜病变占21.62%(107/495),糖尿病肾病占16.36%(81/495),周围神经病变占34.14%(169/495)。与非并发症组患者比较,糖尿病视网膜病变组患者糖尿病病程更长,收缩压(SBP)和糖化血红蛋白(HbA1c)水平更高,低密度脂蛋白胆固醇(LDL-C)水平较低;糖尿病肾病组患者糖尿病病程更长,甘油三酯(TG)和24h尿白蛋白排泄率(UAER)更高;周围神经病变组患者SBP、舒张压(DBP)、HbA1c、总胆固醇(TC)和TG更高,差异均有统计学意义(P<0.05)。多因素logistic回归分析结果显示,糖尿病病程长(OR=3.013,95%CI 1.206~7.526;P=0.019)、高SBP(OR=2.445,95%CI 1.105~5.408;P=0.028)和高HbA1c(OR=3.093,95%CI 1.112~8.606;P=0.031)是并发糖尿病视网膜病变的独立危险因素;糖尿病病程长(OR=2.404,95%CI 1.185~4.877;P=0.016)、高TG(OR=2.654,95%CI 1.241~5.677;P=0.012)和高UAER(OR=3.432,95%CI 1.303~9.036;P=0.013)是并发糖尿病肾病的独立危险因素;高SBP(OR=2.020,95%CI 1.228~3.323;P=0.006)、高DBP(OR=2.560,95%CI 1.109~5.912;P=0.028)、高HbA1c(OR=2.382,95%CI 1.321~4.297;P=0.004)、高TC(OR=2.779,95%CI 1.057~7.303;P=0.039)和高TG(OR=2.266,95%CI 1.237~4.152;P=0.008)是并发周围神经病变的独立危险因素。结论 老年糖尿病患者微血管并发症的患病率较高,糖尿病病程长及SBP、DBP、HbA1c、TC、TG、UAER高是微血管并发症的危险因素,临床需重视。 相似文献
9.
目的 探讨母源性抗旋毛虫抗体的传递途径及其对子鼠感染旋毛虫后肠道排虫的作用。方法 98只昆明小鼠子鼠分为4组,感染母鼠所产子鼠感染母鼠哺乳组（A组）、正常母鼠所产子鼠感染母鼠哺乳组（B组）、感染母鼠所产子鼠正常母鼠哺乳组（C组）及正常母鼠所产子鼠正常母鼠哺乳组（D组）。4组子鼠分别在14、21、42日龄时尾静脉采血,用旋毛虫肌幼虫ES抗原ELISA检测血清抗体水平,然后分别经口感染200条肌幼虫,18 h后剖杀子鼠,计数肠道虫荷。 结果 A、B、C和D等4组14日龄子鼠的肠道平均虫荷分别为5、5、19及18条,21日龄子鼠分别为18、19、75及73条。14日与21日龄的A、B两组子鼠肠道虫荷均显著低于C、D两组子鼠（F14=10.056,F21=35.062,P<0.01）。14日和21日龄子鼠血清吸光度（A492）,A组（0.177、0.235）与B组（0.183、0.250）均显著高于C组（0.108、0.105）与D组（0.067、 0.065） （F14=75.326,F21=60.867,P<0.01）;14日和21日龄4组子鼠肠道虫荷与其血清抗体水平均呈负相关（r14=-0.621,r21=-0.756,P<0.01）。42日龄4组子鼠肠道虫荷差异无统计学意义（F42=0.916,P>0.05）,血清抗旋毛虫抗体均为阴性,肠道虫荷与其血清抗体水平无相关性（r42=-0.291,P>0.05）。 结论 母源性抗旋毛虫抗体主要经乳汁传递,可明显促进14日和21日龄子鼠感染旋毛虫后的肠道排虫反应。 相似文献
10.
The anabolic effects of recombinant human growth hormone and glutamine on parenterally fed,short bowel rats 总被引：10，自引：0，他引：10
AIM：To evaluate the metabolic effects associated with administration of rhGH and/or Gln in parenterally fed,short-bowel rats,Methods:Forty SD rats subjected to 75%intestinal resection and maintained with parenteral nutrition were randomly divided into 4groups as follows:-rhGH,-Gln;-rhGH,+Gln;+rhGH,-Gln:+rhGH,+Gln,Body weight and nitrogen balance were evaluated daily.After 6days of PN,rats were killed,various organs were dissected and weighted,the carcasses were used for analysis of body composition.Serum GH and IGF-1were determined by RIA method.RESULTS:Weight lossin rats with rhGH(17.4±12.8g)and rhGH+Gln(23.8±3.5g)was significantly less than rats with PNalone(29.6±6.9g)and ats with Gln-supplemented PN(31.85±12.8g),P<0.05,The accumulated NBin rats with rhGH(1252。0132294.3mgN/d)and rhGH|Gln(1261.7±85.5mgN/d)was significantly greater than those with PN alone(704.8±379.0mgN/d)and with Gln-supplementedPN(856.7±284.4mgN/d),P<0.05.The absolute weight of gastrocnemius muscle in rats with rhGH(2683.9±341.6mg)andrhGH+Gln(2579.1±359.5mg)was greter than those withPNalone(2176.3±167.1mg)and with Gln-supplementedPN(2141.9±353.6mg).Although the absolute weight of remnant small intestine itself was not significantly different in 4experimental groups,the weight/length of the segments was greater in rats with rhGHand/or Gln(48.7±5.5,52.7±4.1and67.4±5.3respectively)than those with PNalone(47.8±5.0),there were synergistic effects between rhGHand Gln in improvement of the weight/hength of remnant small intestine,P<0.05,Analyses of body carcass composition showed that a higher percentage of carcass weight as protein and a lower percentage of carcass weight as fat were occurred in ats with rhGH(20.8±4.0,6.0±2.6)andrhGH+Gln(21.3±2.4,4.4±1.5)than those with PNalone(16.4±2.4,9.2±3.7)and with Gln-supplemented PN(17.8±3.0,6.3±2.0),rhGH had significant effects on alteration of body composition,P<0.05,Serum GH and IGF-1 concentration in rats with rhGH(5.221±0.8and 425.1±19.2ng/ml respectively)were greater than those with PN alone(3.327±1.7and325.8±29.6ng/ml respectively)and witGln-supplemented PN(3.433±0.1and347.7±55.7ng/ml respectively),P<0.01.CONCLUSION:rhGH significantly improves the anabolism in parenterally fed.short obwel rats,anabolic effect with Gln is less dramatic,there is no synergistic effect between rhGH and Gln in improvement of whole body anabolism.IGF-1 plays an important part in growth-promoting effects of rhGH. 相似文献
11.
［摘要］ 目的 观察水杨酸钠（SS）作用大鼠耳蜗螺旋神经节神经元（SGN）后,SGN中氧化应激水平的变化。方法 将6只SD大鼠随机分为对照组和SS组,每组3只。通过耳蜗器官培养48 h后,用5 mM SS处理48 h,对照组不予处理,采用免疫荧光染色技术定位耳蜗器官中活性氧自由基（ROS）的主要生成位置。将15只SD大鼠随机分成5组,即对照组、SS组、SS+N-乙酰-L-半胱氨酸(NAC)组、阳性对照过氧化氢（H2O2）组、H2O2+NAC组,每组3只。通过急性分离SGN,原代培养48 h后分别用5 mM SS、5 mM SS联合100 μM NAC、300 μM H2O2、300 μM H2O2联合100 μM NAC处理48 h,对照组不予处理。采用荧光染色法检测并量化各组大鼠SGN中ROS荧光探针DCFH-DA的平均荧光强度;采用CCK8法检测SGN细胞存活率。结果 在耳蜗器官培养中,SS作用后,免疫荧光染色显示ROS荧光增强,并且主要在SGN中表达,而在其他细胞中荧光强度无明显变化。为进一步量化荧光强度,在原代培养SGN中加入5 mM SS处理后,荧光染色法显示ROS平均荧光强度较对照组升高（P<0.001）,与H2O2组结果一致（P<0.000 1）。在加入ROS抑制剂NAC之后,ROS平均荧光强度较SS组下降（P<0.01）。CCK8法结果显示,SS作用之后细胞存活率较对照组下降41.34%（P<0.01）;在加入NAC之后,细胞存活率较SS组上升36.05%（P<0.01）,与对照组比较差异无统计学意义（P>0.05）。H2O2组细胞存活率较对照组下降52.31%（P<0.001）,在加入NAC之后,细胞存活率较H2O2组上升34.73%（P<0.01）。结论 SS增强SGN的氧化应激,并且导致了SGN损伤。氧化应激抑制剂NAC可以降低SGN的氧化应激,并且对SS致SGN损伤具有保护作用。 相似文献
12.
Effects of estradiol on liver estrogen receptor-alpha and its mRNA expression in hepatic fibrosis in rats 总被引：5，自引：0，他引：5
Xu JW Gong J Chang XM Luo JY Dong L Jia A Xu GP 《World journal of gastroenterology : WJG》2004,10(2):250-254
AIM:Estradiol treatment regulates estrogen receptor (ER) level in normal rat liver.However,little information is available concerning the role of estrogen in regulating liver ER in hepatic fibrosis in rats.The present study was conducted to determine whether estradiol treatment in CCl4-induced liver fibrosis of female and ovariectomized rats altered liver ERα and its mRNA expression,and to investigate the possible mechanisms.METHODS:Seventy female rats were divided into seven groups with ten rats in each. The ovariectomy groups were initiated with ovariectomies and the sham operation groups were initiated with just sham operations.The CCl4 toxic fibrosis groups received 400mL/L CCI4 subcutaneously at a dose of 2 mL/kg twice weekly.Estrogen groups were treated subcutaneously with estradiol 1mg/kg, the normal control group and an ovariectomy group received injection of peanut oil vehicle twice weekly.At the end of 8 weeks,all the rats were killed to detect their serum and hepatic indicators,their hepatic collagen content, and liver ER and ER mRNA expression.RESULTS: Estradiol treatment in both ovariectomy and sham ovariectomy groups reduced liver levels of ALT (from 658±220nkat/L to 311±146nkat/L and 540±252nkat/L to 314±163nkat/L,P<0.05) and AST (from 697±240nkat/L to 321±121nkat/L and 631±268nkat/L to 302±153nkat/L,P<0.05),increased serum nitric oxide (NO) level (from 53.7±17.1μmol/L to 93.3±4.2μmol/L and 55.3±3.1μmol/Lto 87.5±23.6μmol/L, P<0.05) and hepatic nitric oxide synthase (NOS) activity (from 1.73±0.71KU/g to 2.49±1.20KU/g and 1.65±0.46KU/g to 2.68±1.17KU/g, P<0.05),diminished the accumulation of hepatic collagen,decreased centrolobular necrotic areas as well as the inflammatory reaction in rats subjected to CCl4. The positive signal of ER and ER mRNA distributed in parenchymal and non-parenchymal hepatic cells,especially near the hepatic centrolobular and periportal areas.Ovariectomy decreased ER level (from 10.2±3.2 to 4.3±1.3) and ER mRNA expression (from 12.8±2.1 to 10.9±1.3) significantly (P<0.05). Hepatic ER and ER mRNA concentrations were elevated after treatment with estradiol in both ovariectomy (15.8±2.4, 20.8±3.1) and sham ovariectomy(18.7±3.8, 23.1±3.7) fibrotic groups (P<O.05).CONCLUSION: The increase in hepatic ER and mRNA expression may be part of the molecular mechanisms underlying the suppressive effect of estradiol on liver fibrosis induced by CCI4 administration. 相似文献
13.
14.
The effects of anisodamine and dobutamine on gur mucosal blood flow during gut ischemia／reperfusion 总被引：19，自引：0，他引：19
AIM：To detemine if anisodamine is able to augment mucsal perfusion during gut I/R ischemia-reperfusion METHODS:A jejunal sac was formend in Sperague Dawley rat.A Laser Doppler probe and a tonometer were inserted into the sac thich was filled with saline.The superior mesenteric artery was occluded(SMAO)for 60 minutes followed by 90 minutes of reperfusion.At the end of 60 minutes of SMAO.either 0.2mg/kg of anisodmine or dobutamine was injected into the jejunal sac.Lase Doppler mucosal blood flow and regional PCO2(PrCO2) measurements were made .RESULTS:Mucosal blood flow was significantly increased at 30,60 and 90 minutes of reperfusion(R30,R60,R90)when intraluminal anisodamine or dobutamine was present compared to intraluminal saline only(44±3.3%or 48±4.1%vs 37±2.6%at R30,57±5.0%,57±5.0%or56±4.7%vs45±2.7%at R60,64±3.3%or56±4.2%.vs48±3.4%at R90,respectively P<0.05),Blood flow changes were also reflected by lowering of jejunal PrCO2 measurements after imtraluminal anisodamine or dobutamine compared with that of the saline controls(41±3.1mmHg or 44±3.0mmHg vs49±3.7mmHg at R30,38±3.7mmHg or 40±2.1mmHg vs47±3.8mmHg at R60,34±2.1mmHg or 39±3.0mmHg vs46±3.4mmHg at R90,respectively,P<0.05),Most interesting finding was that there were significantly higher mucosal blood flow and lower jejunal PrCO2 in anisodamine group than those in dobutamine group at 90 minutes of reperfusion(64±3.3%vs56±4.2%for blood flow or 34±2.1mmHg vs39±3.0mmHg for PrCOs,respectively,P<0.05).suggesting that anisodamine had a more lasting effect on mucosal perfusion than dobutamine.CONCLUSION.Intraluminal anisodamine and dobutamine can augment mucosal blood flow during gut I/R and alleviate mucosal acidosis.The results provided benifical effects on the treatment of splanchnic hypoperfusion following traumatic or burn shock. 相似文献
15.
Jiao XY Lü MD Huang JF Liang LJ Shi JS 《World journal of gastroenterology : WJG》2004,10(23):3480-3484
AIM: To study the changes of quantitative expression, adhering activity and genomic density polymorphism of complement types in erythrocytes (CR1) of patients with gallbladder carcinoma and the related clinical significance.METHODS: Polymerase chain reaction (PCR), HindⅢ restrdion enzyme digestion, quantitative assay of CR1 and adhering activity assay of CR1 in erythrocytes were used.RESULIFS: The number and adhering actvity of CR1 in patients with gallbladder carcinoma (0.738±0.23, 45.9±5.7) were significantly lower than those in chronic cholecystitis and cholecystolithiasis (1.078±0.21, 55.1±5.9) and healthy controls (1.252±0.31, 64.2±7.4) (P<0.01). The number and adhering activity of CR1 in patients with chronic cholecystitis and cholecystolithiasis (1.078±0.21, 55.1±5.9) were significantly lower than those in healthy controls (1.252±0.31, 64.2±7.4) (P<0.05). There was a positive correlation between quantitative expression and adhering activity of CR1 (r = 0.79, P<0.01).Compared with those on preoperative day (0.738±0.23,45.4±4.9), the number and adhering activity of CR1 in patients with gallbladder carcinoma decreased greatly on the third postoperative day (0.310±0.25, 31.8±5.1) (P<0.01), and on the first postoperative week (0.480±0.25, 38.9±5.2) (P<0.01), but they were increased slightly than those on the preoperative day (P>0.05). The number and adhering activity of CR1 recovered in the second postoperative week (0.740±0.24, 46.8±5.9) (P<0.01) and increased greatly in the third postoperative week (0.858±0.35, 52.7±5.8) (P<0.01) in comparison with those on the preoperative day and in the first postoperative week. The number and adhering activity of CR1 of gallbladder carcinoma patients with infiltrating, adjacent lymphogenous and distant organ metastases were significantly lower than those of gallbladder carcinoma patients without them (P<0.01). No difference was observed between the patients with gallbladder carcinoma and healthy individuals in the spot mutation rate of CR1 density gene (%2 = 0.521, P>0.05).The distribution of expression was 67.8% in high expression genomic type, 24.8% in moderate expression genomic type,and 7.4% in low expression genomic type. The number and adhering activity of CR1 high expression genomic type gallbladder carcinomas (0.749±0.22, 42.1±6.2) were significantly lower than those of healthy individuals(1.240±0.29, 63.9±7.2), and were also significantly lower than those of healthy individuals (0.921±0.23, 54.8±7.1), but no difference was observed between the number and adhering activity of CR1 lower expression genomic type gallbladder carcinomas (0.582±0.18, 44.3*_5.5) and those of healthy individuals (0.610±0.20, 45.8±5.7) (P>0.05).CONCLUSION: Defective expression of CR1 in gallbladder carcinoma is mostly acquired through central peripheral mechanisms. The changes in CR1 quantitative expression and adhering activity are consanguineously related to the development and metastasis in gallbladder carcinoma. 相似文献
16.
目的 通过调查北京市部分社区2型糖尿病患者降糖用药情况,了解不同版本《国家基本药物目录》中降糖药物(简称国家基本降糖药物)的覆盖情况。方法 纳入北京市4个社区卫生服务中心的2型糖尿病患者900例,以糖化血红蛋白(HbA1c)<7%为目标控制血糖,随访1年后比较不同版本国家基本降糖药物使用覆盖率的变化。结果 (1)基线时:应用2012年版国家基本降糖药物的比例(91.4%)明显高于应用2009年版目录药物的比例(42.9%)(χ2=481.09,P<0.05)。7种国家基本降糖药物中,阿卡波糖(48.9%)、二甲双胍(40.7%)和重组人胰岛素(31.1%)的覆盖率较高(χ2=1519.65,P<0.05)。(2)1年后随访:总人群HbA1c达标率(53.6%)较基线时(32.3%)提高(χ2=77.26,P<0.05)。应用2012年版国家基本降糖药物的比例(85.5%)仍明显高于应用2009年版目录药物的比例(37.4%)(χ2=376.367,P<0.05)。阿卡波糖(49.7%)、二甲双胍(36.3%)和重组人胰岛素(30.4%)的覆盖率仍较高(χ2=1320.70,P<0.05)。结论 2012年版的国家基本降糖药物较2009年版可更好地满足北京社区2型糖尿病患者的需求。
相似文献
相似文献
17.
本文根据国外蚊虫分类经验,对我国伊蚊族蚊类以往记录171种的分类地位作了校订,其中160种可归入我国伊蚊族新分类系统的29个属中,另外11种地位未定,暂按旧分类地位列名表供查考。我国伊蚊族新分类系统包括伊蚊属Aedes、阿蚊属Armigeres、艾蚊属Ayurakitia、博蚊属Bothaella*、布蚊属Bruceharrisonius*、环喙蚊属Christophersiomyia*、科蚊属Collessius*、丹蚊属Danielsia*、唐蚊属Downsiomyia*、箭阳蚊属Edwardsaedes*、纷蚊属Finlaya*、弗蚊属Fredwardsius*、贾蚊属Gilesius*、领蚊属Heizmannia、喜蚊属Himalaius*、霍金蚊属Hopkinsius*、呼蚊属Hulecoeteomyia*、连蚊属Jihlienius*、奈蚊属Kenknightia*、陆蚊属Luius*、霉蚊属Mucidus*、新黑蚊属Neomelaniconion*、骚扰蚊属Ochlerotatus、花蚊属Phagomyia*、盾蚊属Scutomyia*、覆蚊属Stegomyia*、田中蚊属Tanakaius*、尤蚊属Udaya和奇阳蚊属Verrallina等29属,其中22属（*）为我国新记录。此外,还对《中国动物志,昆虫纲第8卷,双翅目：蚊科上卷》记载的4种伊蚊作了重要订正。安图伊蚊Ae. （Edw.） antuensis应是平坝箭阳蚊Ed. pingpaensis的同物异名。滇西伊蚊Ae. （Sin.） occidentayunnanus、黄背伊蚊Ae. （Och.） flavidorsalis和亚同伊蚊Ae. （Fin.） subsimilis分别订正为滇西领蚊Hz. （Mat.） occidentayunnana、白色骚扰蚊Oc. albineus和亚同尤蚊Ud. subsimilis。 相似文献
18.
19.
Variability of cell proliferation in the proximal and distal colon of normal rats and rats with dimethylhydrazine induced carcinogenesis 总被引：6，自引：0，他引：6
AIM：To investigate the patterns of cell proliferation in proximal and distal colons in normal rats and rats with1,2-dimethylhydrazine(DMH)induced carcinogenesis using the thymidine analogue bromodeoxyuridine.METHODS:Colonic crypt cell proliferation was immunohistochemically detected using the anti-bromodeoxyuridine Bu20a monoclonal antibody.RESULTS:Marked regional differences were found in both groups.Total labelling index(LI)and proliferative zone size in both normal(8.65±0.34vs7.2±0.45,27.74±1.07vs16.75±1.45)andDMH groups(13.13±0.46vs11.55±0.45,39.60±1.32vs35.52±1.58)were significantly higher in distal than in proximal colon(P<0.05).although the number of cells per proxmal crypt was greater(31.45±0.20vs34.45±0.39,42.68±0.53vs49.09±0.65,P<0.001).Crypt length,total LT and proliferative zone size all increased in both proximal and distal regions of DMH rats compared to normal controls(P<0.0001).In DMH-treated rat colon a shift of labelled cells to higher crypt cell positions was demonstrated distally whist a bi-directional shift was evident proximally(P<0.05).CONCLUSION:Our results show that changes in cell proliferation patterns,as assessed by bromodeoxyuridine uptake,can act as a reliable intermediate marker of colonic cancer formation.Observed differences between proliferation patterns in distal and proximal colon may be associated with the higher incidence of tumors in t he distal colon. 相似文献
20.
目的 探讨橙皮素(HES)对H2O2诱导的H9c2心肌细胞凋亡有何影响。方法 采用H2O2建立H9c2心肌细胞氧化应激损伤模型,实验分为四组：正常对照组(Control组),H2O2损伤组(H2O2组),单纯橙皮素处理组(HES组),橙皮素预处理 H2O2组(HES H2O2组)。H2O2(400 μM)处理2 h建立心肌细胞氧化应激损伤模型,HES H2O2组于建模前1 h加入40 μM橙皮素。采用CCK-8法确定H9c2细胞活性；DCFH-DA 探针检测细胞活性氧簇(ROS)水平,流式细胞术检测心肌细胞凋亡,分光光度计检测Caspase-3活性。结果 橙皮素预处理可明显改善H2O2诱导的H9c2心肌细胞活性降低,并且呈浓度依赖性；给予40 μM橙皮素预处理后ROS的产生明显减少,Caspase-3活性显著下降,心肌细胞凋亡率下降到30%左右。结论 橙皮素对氧化应激诱导的心肌细胞凋亡具有抑制效应。 相似文献
