孕早期子痫前期孕妇血胎盘生长因子、微小核糖核苷酸水平及风险预测价值

目的：分析血浆胎盘生长因子(PIGF)与微小核糖核苷酸-240(miRNA-240)在孕早期子痫前期(PE)患者血中的表达，并建立疾病预测模型。方法：收集2020年1月-2021年6月本院产前检查并分娩的孕产妇临床资料，诊断PE 130例为病例组，其中早发型PE 70例、晚发型PE 60例，正常孕妇150例为对照组。均于孕11～13⁺⁶周产前检查唐氏血清筛查时检测血浆PIGF及血清miRNA-204水平；绘制受试者工作特征曲线分析血浆PIGF及血清miRAN-204预测PE价值。结果：病例组孕早期血浆PIGF(136.98±16.78 pg/ml)低于对照组(179.58±20.13 pg/ml),外周血miRNA-240-5p(2.11±0.41)高于对照组(1.03±0.32)(P<0.001),但病例组中早发型PE与晚发型PE患者均未见差异(P>0.05)。病例组血浆PIGF与血清miRNA-204相对表达量呈负相关(P<0.001)。绘制ROC曲线，孕早期血浆PIGF、血清miRNA-204预测PE均有较好效能，曲线下面积(AUC)分别为...     

高危型HPV阳性宫颈癌患者miRNA-34a-5p的表达及临床意义

目的　探讨高危型人乳头状瘤病毒（high risk-human papilloma virus, HR-HPV）阳性宫颈癌患者组织及血清中微小RNA（microRNA, miRNA）-34a-5p的表达及其对宫颈癌的诊断价值。方法　将100例HR-HPV阳性宫颈癌患者（宫颈癌组）、70例宫颈上皮内瘤变（cervical intraepithelial neoplasia, CIN）患者（CIN组）、70例HR-HPV阳性子宫良性病变或者HPV单纯阳性的健康体检者（对照组）作为研究对象。采集上述研究对象的血清标本及70例行手术治疗的宫颈癌组患者癌组织及癌旁组织。使用实时荧光定量PCR技术测定组织及血清中miRNA-34a-5p水平，并分析miRNA-34a-5p与宫颈癌临床、病理指标的关联及血清miRNA-34a-5p对宫颈癌的诊断价值。结果　①对照组血清中miRNA-34a-5p的相对表达量高于CIN组和宫颈癌组：（0.933±0.097）vs.（0.554±0.099）vs.（0.369±0.099），差异有统计学意义（F=741.401，P=0.000）；宫颈癌组患者术前血清中miRNA-34a-5p相对表达量低于术后1周，差异有统计学意义（t=8.305，P=0.000）。有无淋巴结转移和有无远处转移的宫颈癌患者血清中miRNA-34a-5p相对表达量的差异有统计学意义（P均＜0.05）。②宫颈癌组患者癌旁组织中miRNA-34a-5p的相对表达量高于癌组织，差异具有统计学意义（t=40.313，P=0.000）。③宫颈癌组患者癌组织中的miRNA-34a-5p表达水平与血清中的表达水平呈正相关（r=0.908，P=0.000）。④以0.508为miRNA-34a-5p相对表达量的最佳临界值诊断宫颈癌，其灵敏度为83.6%，特异度为79.4%，AUC为0.860，95%置信区间为0.803～0.917。结论　HR-HPV阳性宫颈癌患者癌组织和血清中miRNA-34a-5p表达下调，对宫颈癌诊断及预后监测具有一定的参考价值。     

妊娠高血压疾病患者血清miR-155-5p、LncRNA MALAT1表达及与糖脂代谢关系

目的：探讨微小RNA-155-5p(miR-155-5p)、长链非编码RNA肺癌转移相关转录本1(LncRNA MALAT1)在妊娠期高血压疾病患者血清中的表达及与糖脂代谢关系。方法：选择2019年8月-2021年12月本院收治的103例妊娠期高血压疾病患者为病例组，其中妊娠期高血压患者41例、轻度子痫前期患者35例、重度子痫前期患者27例，选择同期产前检查正常孕妇40例为对照组，qRT-PCR检测血清miR-155-5p、LncRNA MALAT1表达，检测血清糖脂代谢指标水平，Pearson法分析血清miR-155-5p与LncRNA MALAT1表达相关性，及与血糖(FBG)、糖化血红蛋白(HbA1c)、胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)水平的相关性。结果：与对照组比较，妊娠期高血压组、轻度子痫前期组、重度子痫前期组患者血清miR-155-5p、FBG、HbA1c、TG、TC、LDL-C水平升高，且随疾病程度增加而升高，LncRNA MALAT1、HDL-C水平降低，且随疾病程度增加而降低(均P<0.05);相关性分析显...     

微小RNA-140-5p靶向EGR-2基因调控胃癌细胞增殖凋亡的作用研究

目的 探讨microRNA-140-5p(miRNA-140-5p)在胃癌患者组织中的表达及其对癌细胞增殖、凋亡的影响。 方法 收集2017年5月到2018年5月杭州市肿瘤医院住院的100例胃癌患者的组织样本,利用荧光定量PCR检测miRNA-140-5p在胃癌组织和癌旁组织中的表达并分析其临床意义。采用CCK-8法、Annexin V-FITC/PI双染法分析miRNA-140-mimics、miRNA-140-inhibitor对人胃癌细胞系BGC-823增殖和凋亡的影响,以及检测其对胃癌细胞TGF-β、ERK1、Smad2、IGF-1R、PI3K、Ras、AKT、MAPK、Ki67基因表达的影响。采用生物信息学、Western Blotting、双荧光素酶报告实验等方法预测并验证miRNA-140-5p的下游靶基因。 结果 胃癌患者癌组织中miRNA-140-5p的表达水平显著低于癌旁组织(P<0.001);ROC曲线示miRNA-140-5p诊断胃癌的曲线下面积为0.803(95%CI:0.723～0.851);与对照组相比,miRNA-140-mimics组中的细胞增殖率明显降低而凋亡率升高(均P<0.05);TGF-β,ERK1,Smad2,IGF-1R,PI3K,Ras,AKT,MAPK mRNA表达,以及Ki67蛋白表达明显下调(P<0.05);Targetscan数据库预测和双萤光素酶检验结果发现早期生长反应蛋白2(EGR2)是miRNA-140-5p的下游靶基因之一,较于空白对照组,miRNA-140-mimics组中EGR2含量降低(P<0.001)。 结论 miRNA-140-5p在胃癌组织中表达下调,可能通过靶向EGR2来调控胃癌细胞的增殖与凋亡, miRNA-140-5p有可能成为胃癌诊断和新型生物治疗的靶点。     

miRNA-127-5p及sRAGE和IL-10预测重症肺炎临床转归不良的临床价值

目的 分析重症肺炎感染病原菌分布及微小核糖核酸(miRNA)-127-5p、可溶性晚期糖基化终产物受体(sRAGE)、白细胞介素-10(IL-10)预测重症肺炎临床转归不良的临床价值。方法 选取2019年6月-2022年9月连云港市东方医院收治的299例重症肺炎患者，根据患者临床转归分为临床转归良好组235例，临床转归不良组64例。分析重症肺炎感染病原菌分布，采用受试者工作特征(ROC)曲线分析支气管肺泡灌洗液miRNA-127-5p相对表达量和外周血IL-10、sRAGE对重症肺炎临床转归不良的预测价值。结果 299例重症肺炎患者临床转归不良64例，临床转归不良率为21.40%。共分离出病原菌342株，其中革兰阴性菌161株，占比为47.08%;临床转归不良组支气管肺泡灌洗液miRNA-127-5p相对表达量，血清IL-10、sRAGE水平均高于临床转归良好组(P<0.05); ROC曲线分析结果显示，miRNA-127-5p, IL-10、sRAGE预测重症肺炎患者临床转归不良的曲线下面积(AUC)分别为0.821、0.812、0.801。结论 重症肺炎患者临床转归不良发生率...     

外周血MicroRNA-6767-5p在多囊卵巢综合征患者中的表达及临床意义

目的通过检测多囊卵巢综合征(PCOS)外周血miRNAs的表达量,探讨其在PCOS患者中的表达情况及临床意义。方法选取2015年12月-2016年12月在东莞市石排医院妇科收治的PCOS确诊患者21例为PCOS组,另选取同期在该院进行体检的21名健康女性作为对照组。采用Sure Print G3生物芯片检测miRNAs的表达,选取PCOS患者中上调超过1. 5倍或下调少于0. 67倍的miRNA,然后采用定量实时聚合酶链反应(RT-q PCR)验证miRNA的相对表达。结果与健康对照相比,PCOS患者血清中miRNA-4522,miRNA-324-3p和miRNA-6767-5p表达量下调;经RT-q PCR验证,PCOS/对照组miRNA-6767-5p表达量比例为0. 39 (P0. 05);经调整年龄、体重指数后,miRNA-6767-5p与空腹血糖呈负相关(β=-0. 371,P0. 05),与每年月经次数呈正相关(β=0. 383,P0. 05); miRNA-6767-5p基因靶向基因参与细胞周期和免疫系统。结论血清miRNA-6767-5p可作为PCOS诊断的分子生物学标志物,可能参与PCOS患者的新陈代谢和生殖功能。     

miRNA-34a和癌胚抗原在良恶性胸腔积液鉴别诊断中的应用价值

目的 探讨miRNA-34a和癌胚抗原(CEA)的检测在良恶性胸腔积液鉴别诊断中的应用价值。方法 收集良恶性胸腔积液患者各35例，采用实时荧光定量PCR测定miRNA-34a的相对表达量，全自动免疫分析仪测定血清CEA水平，应用ROC曲线评估miRNA-34a和CEA的诊断性能。结果 恶性胸腔积液组患者miRNA-34a相对表达量低于良性胸腔积液组，差异有统计学意义(P<0.01);恶性胸腔积液组患者CEA浓度高于良性胸腔积液组，差异有统计学意义(P<0.01)。ROC曲线分析，miRNA-34a和CEA诊断恶性胸腔积液曲线下面积(AUC)分别为0.704和0.764。结论 miRNA-34a在恶性胸腔积液中呈低表达，可能与相关肿瘤的胸膜转移有关。胸腔积液中miRNA-34a和CEA检测对恶性胸腔积液的诊断具有一定的辅助性价值。     

前列腺增生症术后尿路感染患者血清炎症因子、miRNA-15a、miRNA-34b和miRNA-103及CYP1A2基因多态性研究

目的探讨前列腺增生症术后尿路感染患者血清炎症因子、微小RNA(microRNA,miRNA)-15a、miRNA-34b和miRNA-103及CYP1A2基因多态性相关性。方法选择浙江省医疗健康集团杭州医院于2017年7月-2019年7月收治的前列腺增生症患者112例,均行经尿道前列腺等离子双极电切术(Transurethral plasmakinetics resection of prostate,TUPKRP),根据是否发生术后尿路感染分为感染组(n=27)与非感染组(n=85)。分析两组患者血清炎症因子,外周血miRNA-15a、miRNA-34b和miRNA-103表达,及CYP1A2基因多态性变化。结果感染组血清CRP、TNF-α、IL-6和PCT水平高于非感染组(P<0.05)。感染组外周血miRNA-15a表达低于未感染组,而miRNA34b和miRNA103表达高于未感染组(P<0.05)。感染组CYP1A2基因位点rs2069514-3859等位基因A分布高于非感染组,而G分布低于非感染组(P<0.05)。感染组CYP1A2基因位点rs20695143859基因型AA分布高于非感染组(P<0.05)。结论前列腺增生症术后尿路感染患者血清炎症因子CRP、TNF-α、IL-6和PCT水平升高,血miRNA-15a低表达及miRNA34b和miRNA103高表达参与了术后尿路感染发生、发展,且CYP1A2基因位点rs20695143859等位基因A可能为术后尿路感染易感基因。     

妊娠期高血压患者HLA-Ⅰ、miR-95-5p表达及与母婴不良结局相关性

目的:分析妊娠期高血压患者血清白细胞抗原Ⅰ类分子(HLA-Ⅰ)、微小RNA-95-5p(miR-95-5p)表达及与母婴结局相关性.方法:选取2019年1月-2020年1月本院收治的妊娠期高血压患者95例为高血压组,产前检查正常妊娠孕妇80例为健康组,酶联免疫吸附测定法检测血清白细胞抗原-Ⅰ (sHLA-Ⅰ)水平,We...     

孕妇人体质量指数、C反应蛋白和糖化血红蛋白与妊娠期糖尿病的关系及预测意义

目的探讨孕妇人体质量指数(BMI)、C反应蛋白(CRP)及糖化血红蛋白(HbA1c)水平，与其发生妊娠期糖尿病(GDM)的关系，以及上述3项指标对预测GDM的价值。 方法选择2016年12月至2018年12月，于南京市中西医结合医院进行干预治疗和分娩的100例GDM孕妇为研究对象，将其纳入GDM组；选择同期与GDM组在同一家医院进行产前检查和分娩的60例健康孕妇作为对照，纳入对照组。采用成组t检验和χ²检验，对2组孕妇空腹血糖(FPG)、餐后2 h血糖(2 h PPG)、BMI、CRP及HbA1c水平，以及2组孕妇围生结局指标的发生率，进行统计学比较；采用Pearson相关性分析，对GDM组孕妇的BMI、CRP、HbA1c分别与其FPG及2 h PPG的相关性进行分析。本研究遵循的程序，符合2013年修订的《世界医学协会赫尔辛基宣言》要求，与所有受试者均签署临床研究知情同意书。 结果① GDM组孕妇的FPG、2 h PPG、BMI、CRP及HbA1c水平分别为(7.8±1.0) mmol/L、(11.0±1.6) mmol/L、(25.8±1.2) kg/m²、(12.8±2.9) mg/L及(8.5±1.7)%，均显著高于对照组孕妇的(5.6±0.6) mmol/L、(7.6±1.1) mmol/L、(22.2±1.1) kg/m²、(7.7±2.4) mg/L及(4.0±1.0)%，并且差异均有统计学意义(t＝14.139、13.394、17.455、10.726、16.705，均为P<0.001)。② Pearson相关性分析结果显示，GDM孕妇的BMI、CRP及HbA1c水平分别与FPG、2 h PPG，均呈正相关关系(r＝0.438、0.408，P<0.001；r＝0.389、0.410，P<0.001；r＝0.413、0.442，P<0.001)。③GDM组孕妇早产、胎膜早破、羊水过多、分娩巨大儿、胎儿窘迫及产后出血发生率，分别为21.1%、23.3%、22.2%、17.8%、16.7%、12.2%，均显著高于对照组孕妇的6.0%、8.0%、6.0%、4.0%、4.0%、2.0%，并且差异均有统计学意义(χ²＝5.542、P＝0.019，χ²＝5.152、P＝0.023，χ²＝6.161、P＝0.013，χ²＝5.446、P＝0.020，χ²＝4.834、P＝0.028，χ²＝4.286、P＝0.038)。GDM组孕妇分娩新生儿的1 min Apgar评分为(7.1±0.7)分，低于对照组健康孕妇分娩新生儿的(8.3±1.0)分，并且差异有统计学意义(t＝8.469，P<0.001)。 结论孕妇BMI、CRP及HbA1c水平与GDM相关，应加强对孕妇这3项指标的早期监测，以早期预测GDM的发生。     

Abundance of ADAM-8, -9, -10, -12, -15 and -17 and ADAMTS-1 in mouse uterus during the oestrous cycle

The aim of the present study was to determine whether a disintegrin and metalloproteinase (ADAM)-8, -9, -10, -12, -15 and -17 and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-1 are involved in the remodelling process of the mouse uterus during the oestrous cycle. The mRNA expression of ADAM was observed in all uterine tissues throughout the entire cycle. The levels of ADAM-8 mRNA were maximal at pro-oestrus, whereas the expression of ADAM-9 and ADAMTS-1 mRNA was maximal at oestrus. The minimum mRNA level of all ADAM genes always occurred at dioestrus. The mRNA levels of ADAM-10, -12, -15 and -17 did not vary significantly, regardless of the stage of the oestrous cycle. Immunoblot analyses demonstrated the presence of all ADAM proteins throughout the cycle. In terms of protein intensities, ADAM-8, -12 and -17 were maximal at pro-oestrus, whereas ADAM-10 and ADAMTS-1 were maximal at metoestrus and ADAM-9 was maximal at oestrus. Regardless of the ADAM species, minimal protein expression always occurred at dioestrus. Immunohistochemical studies showed ADAM protein expression in luminal and glandular epithelial layers, but not in the stromal layer. Moreover, ADAM proteins were found to be heterogeneously localised and their individual localisations depended on the stage of the oestrous cycle. From these observations, we suggest that the ADAM genes play an important role in mouse uterine tissue remodelling during the oestrous cycle.     

Surveillance of influenza in Apulia, Italy, 1999-2000, 2000-2001, 2001-2002, and 2002-2003 seasons

BACKGROUND: The objective of this study was to evaluate, within the Italian National Influenza Epidemiological and Virological Surveillance, the rate of vaccination coverage, the incidence of Influenza Like-Illness (ILI), the incidence of Acute Respiratory Illness (ARI), and to identify the virus strains circulating in Apulia from 1999 to 2003. METHODS: Vaccination coverage rates were calculated based on the number of doses administered to individuals > 65 years of age. Every week, sentinel physicians reported ILI and ARI cases having occurred among their patients. Voluntary general practitioners (GPs) and paediatricians (Ps) collected oropharyngeal swab samples from patients suspected with ILI. Influenza viruses were isolated and identified by cell culture (MDCK cells) and RT-PCR. Virological surveillance was carried out by the ISS, in collaboration with a network of peripheral laboratories. RESULTS: In Apulia, vaccination coverage progressively increased to 68.6% during the 2002-2003 season. The analysis of ILI cases showed higher incidence rates during the 1999-2000 and 2002-2003 seasons. ARI rates appeared to have a more constant trend. ILI and ARI incidence rates were higher in the 0-14 year age group. CONCLUSION: The increase in vaccination coverage rates and implementation of the network of clinical, and epidemiological and virological surveillance are fundamental for the control and prevention of influenza.     

MicroRNA-21的生物学功能及其与肿瘤关系的研究进展

microRNA-21(miR-21)编码基因定位于TMEM49基因编码区域内(17q23.2),通过其自身的启动区域完成转录及polyA尾的聚合,最终变成成熟的miRNA。miR-21与多种疾病相关。miR-21可作为一种肿瘤RNA,其基因簇对多种肿瘤具有促进作用,在肿瘤的发生和发展中有类似癌基因作用,参与肿瘤血管形成,与肿瘤细胞的增殖、侵袭及抗药性密切相关。miR-21也在心脏疾病及肺纤维化疾病中起重要作用。此外,miR-21还可以调节免疫过程,对不同信号传导的靶蛋白起关键作用。随着对miR-21功能研究的不断深入,miR-21可能成为早期诊断和基因治疗新的分子靶标。     

Heat-related mortality--Arizona, 1993-2002, and United States, 1979-2002

Hyperthermia is the elevation of body temperature resulting from the body's inability to dissipate heat. Continued exposure to ambient heat close to body temperature (98.6 degrees F [37.0 degrees C]) contributes to a substantial number of deaths from hyperthermia, especially among elderly persons. To assess the health risk from hyperthermia, Arizona health practitioners and CDC researched cases of heat-related death and illness in Arizona, used U.S. death certificate data to summarize trends in heat-related deaths, and compared age-specific, heat-related death rates in Arizona with those in the United States overall. Findings indicated that, during 1979-2002, a total of 4,780 heat-related deaths in the United States were attributable to weather conditions and that, during 1993-2002, the incidence of such deaths was three to seven times greater in Arizona than in the United States overall. Public health agencies in communities affected by periods of extreme heat should educate populations at risk (e.g., persons aged > or = 65 years) and consider designing and implementing location-specific heat response plans (HRPs).     

Hypothermia-related deaths--Utah, 2000, and United States, 1979-1-998

Hypothermia is a medical emergency that is completely preventable. Hypothermia occurs when persons are exposed to ambient cold temperatures without appropriate protection for extended periods of time. The clinical definition of hypothermia is a core body temperature < or = 95 F (35 C). This report describes cases of hypothermia-related deaths in Utah during 2000 and describes unintentional hypothermia-related deaths in the United States during 1979-1998. The Utah cases illustrate risk factors and environmental conditions associated with hypothermia.     

Surveillance for influenza--United States, 1994-95, 1995-96, and 1996-97 seasons.

PROBLEM/CONDITION: Influenza epidemics occur nearly every year during the winter months and are responsible for substantial morbidity and mortality in the United States, including an average of approximately 114,000 hospitalizations and 20,000 deaths per year. REPORTING PERIOD: This report summarizes U.S. influenza surveillance data from October 1994 through May 1997, from both active and passive surveillance systems. DESCRIPTION OF SYSTEM: During the period covered, CDC received weekly reports from October through May from a) state and territorial epidemiologists on estimates of local influenza activity, b) approximately 140 sentinel physicians on their total number of patient visits and the number of cases of influenza-like illness (ILI), and c) approximately 70 World Health Organization (WHO) collaborating laboratories in the United States on weekly influenza virus isolations. WHO collaborating laboratories also submitted influenza isolates to CDC for antigenic analysis. Throughout the year, vital statistics offices in 121 cities reported deaths related to pneumonia and influenza (P&I) weekly, providing a measure of the impact of influenza on mortality. RESULTS: During the 1994-95 influenza season, 25 state epidemiologists reported regional or widespread activity at the peak of the season. Cases of ILI reported by sentinel physicians exceeded baseline levels for 4 weeks, peaking at 5%. Influenza A(H3N2) was the most frequently isolated influenza virus type/subtype. The longest period of sustained excess mortality was 5 consecutive weeks, when the percentage of deaths attributed to P&I exceeded the epidemic threshold, peaking at 7.6%. During the 1995-96 season, 33 state epidemiologists reported regional or widespread activity at the peak of the season. ILI cases exceeded baseline levels for 5 weeks, peaking at 7%. Influenza A(H1N1) viruses predominated, although influenza A(H3N2) and influenza B viruses also were identified throughout the United States. P&I mortality exceeded the epidemic threshold for 6 consecutive weeks, peaking at 8.2%. The 1996-97 season was the most severe of the three seasons summarized in this report. Thirty-nine state epidemiologists reported regional or widespread activity at the peak of the season. ILI reports exceeded baseline levels for 5 consecutive weeks, peaking at 7%. The proportion of respiratory specimens positive for influenza peaked at 34%, with influenza A(H3N2) viruses predominating. Influenza B viruses were identified throughout the United States, but only one influenza A(H1N1) virus isolate was reported overall. The proportion of deaths attributed to P&I exceeded the epidemic threshold for 10 consecutive weeks, peaking at 9.1%. INTERPRETATION: Influenza A(H1N1), A(H3N2), and B viruses circulated during 1994-1997. Local surveillance data are important because of geographic and temporal differences in the circulation of influenza types/subtypes. PUBLIC HEALTH ACTIONS: CDC conducts active national surveillance annually from October through May for influenza to detect the emergence and spread of influenza virus variants and monitor the impact of influenza-related morbidity and mortality. Surveillance data are provided weekly throughout the influenza season to public health officials, WHO, and health-care providers and can be used to guide prevention and control activities, vaccine strain selection, and patient care.     

“望闻问切”与内部审计

中医的望闻问切辨证论治理论与审计理论有相通之处,如何将此理论应用于内部审计工作,可借鉴之处很多。     

Heat-related deaths--Chicago,Illinois, 1996-2001, and United States, 1979-1999

Heat waves (i.e., >/=3 consecutive days of air temperatures >/=90 degrees F [>/=32.2 degrees C]) are meteorologic events that contribute significantly to heat-related deaths. Exposure to excessive heat can cause illness, injury, and death. This report describes four cases of heat-related deaths, as reported by the Office of the Medical Examiner, Cook County, Chicago, that occurred during 1996-2001; summarizes total heat-related deaths in Chicago during 1996-2001; and compares the number of heat-related deaths during the 1995 and 1999 Chicago heat waves. This report also summarizes trends in the United States during 1979-1999, describes risk factors associated with heat-related deaths and symptoms, and outlines preventive measures for heat-related illness, injury, and death. Persons at risk for heat-related death should reduce strenuous outdoor activities, drink water or nonalcoholic beverages frequently, and seek air conditioning.     

轮状病毒肠炎IL-1β、IL-6、IL-12、TNF-α的变化及意义

目的探讨婴幼儿轮状病毒肠炎白介素1β(IL-1β)、白介素6(IL-6)、白介素12(IL-12)以及肿瘤坏死因子α(TNF-α)的水平变化及意义。方法采用ELISA双抗体夹心法检测轮状病毒肠炎患儿及正常对照组血清、大便上清液IL-1β、IL-6、IL-12以及TNF-α水平。结果急性期轮状病毒性肠炎患儿的血清和大便IL-1β、IL-6、IL-12以及TNF-α均高于对照组(P<0.05)。中、重症组急性期血清和大便IL-1β、IL-6、IL-12以及TNF-α显著高于轻症组(P<0.05)。恢复期各组之间差异无统计学意义(P>0.05)。结论轮状病毒感染后周围血循环和肠粘膜局部产生IL-1β、IL-6、IL-12以及TNF-α。IL-1β、IL-6、IL-12以及TNF-α在RV感染的免疫防御保护和免疫损伤机理中可能起重要作用。