纳米载银室温固化PMMA材料对雄性小鼠生殖系统的影响

目的:研究添加2%浓度纳米载银无机抗菌剂的室温固化型聚甲基丙烯酸甲酯(PMMA)材料对雄性小鼠生殖系统的影响。方法:将雄性昆明小鼠随机分组,动物实验模型建立完成后,处死。计算小鼠体重增长率、睾丸脏器系数,观察睾丸组织的病理学改变,进行精子活动率检测、计数和畸形率计算。采用初级精母细胞染色体畸变实验和精原细胞姊妹染色单体互换(SCE)实验检测实验材料对小鼠初级精母细胞及精原细胞染色体的影响。结果:添加2%浓度纳米载银室温固化PMMA材料对小鼠体重增长率、睾丸脏器系数以及精子计数、活动率和畸形率均无显著性影响,各实验组睾丸组织病理切片与阴性对照组无明显差异。添加2%浓度纳米载银室温固化PMMA材料对小鼠的初级精母细胞染色体无致畸变作用;各实验组精原细胞SCE率与阴性组无显著性差异。结论:添加2%浓度纳米载银室温固化PMMA材料对雄性小鼠生殖系统无显著影响。     

口腔义齿打磨钛合金粉尘致鼠肺纤维化研究

目的研究钛合金义齿打磨粉尘致鼠肺纤维化作用。方法采用大鼠非暴露式气管内注入染尘,染尘后1个月处死动物,取肺组织作病理切片观察,血清铜蓝蛋白测定以及羟脯氨酸含量测定。结果染钛合金粉尘大鼠(20 g/L和10 g/L钛合金组)的血清铜蓝蛋白和羟脯氨酸测定结果与对照组相比差异均有显著性,病理切片显示各实验组大鼠肺组织内均有轻度纤维化改变,其程度与染尘剂量一致。结论钛合金义齿打磨粉尘可致大鼠肺轻度纤维化。     

小鼠切牙干槽症模型建立的实验研究

目的 建立干槽症的小鼠模型.方法 取10只小鼠,随机分为实验组和对照组.每只小鼠腹腔注射1.5％戊巴比妥麻醉后,拔除上颌中切牙.实验组拔牙窝行肾上腺素止血,植入金黄色葡萄球菌标准菌种,对照组拔除上颌中切牙后无特殊处理.4天后观察两组拔牙窝情况并处死小鼠,取上颌骨进行组织切片、HE染色,观察两组拔牙窝的结构变化.结果 拔牙4天后,实验组拔牙窝见大量脓性分泌物,周围黏膜红肿,对照组拔牙窝粘膜呈粉红色,创口较拔牙后明显缩小,无明显分泌物.观察组织学HE染色切片发现,实验组拔牙窝可见大量炎性细胞及少量破骨细胞,对照组的拔牙窝内有新生结缔组织形成.结论 使用肾上腺素和金黄色葡萄球菌标准菌种可以成功地建立小鼠切牙拔牙窝的干槽症模型.     

聚合方式对义齿基托树脂中残余甲基丙烯酸甲酯单体含量的影响

目的研究聚合方式对义齿基托树脂中残余甲基丙烯酸甲酯含量的影响。方法按照不同的聚合方式,将实验分为3组,每组制备3块试验样块.按照YY0270-2003规定的气相色谱法实验方法测试各组样品的甲基丙烯酸甲酯单体（MMA）含量。结果不同聚合方式得到的材料中MMA的百分含量明显不同,以微波聚合方式得到的基托材料残余单体含量最低。结论聚合方式对义齿基托材料残余单体含量具有显著影响。     

一次性18Gy放射对大鼠颌下腺损伤的实验研究

目的:观察一次性18 Gy放射对大鼠颌下腺组织学形态和唾液流率的改变。方法:40只大鼠随机分为实验组和对照组,每组20只。实验组一次性18 Gy局部照射大鼠颌下腺区域,对照组只麻醉不放射。8周后处死所有大鼠,处死前插管法提取大鼠颌下腺唾液,称取其质量,计算唾液流率,比较两组唾液流率的改变。处死后取颌下腺组织,经4%多聚甲醛固定,切片,HE染色,镜下观察颌下腺的组织学形态。结果:放射后实验组进食进水量下降、活动减少。实验组饮水频率高于对照组。对照组的唾液流率为(18.64±8.23)μL/min,实验组的唾液流率是对照组的57.42%,为(10.70±2.22)μL/min。HE染色显示,放射后实验组颌下腺细胞变性,间质血管充血,腺泡细胞内的空泡数量明显增多。结论:放射后大鼠颌下腺唾液流率明显降低,放射后8周,组织学形态出现显著变化。放疗对大鼠颌下腺组织学形态和唾液分泌功能的远期影响,尚需进一步观察和研究。     

颞下颌关节盘摘除术关节区组织病理学变化的实验研究

目的 建立兔颞下颌关节盘摘除术实验动物模型,研究关节盘摘除术早期,关节区组织形态学变化.方法 用10只新西兰大白兔,实验组8只行双侧关节盘摘除术;2只为正常对照组.术后1周、2周、4周、10周各处死2只,切取关节组织,进行组织病理学观察.结果 髁突及关节结节关节软骨连续性破坏,功能区关节软骨下骨组织直接暴露于关节腔内,非功能区则软骨细胞各层增生明显,表面纤维层增生变厚,呈现出纤维性粘连样改变.暴露在关节腔部分的骨组织表面致密,髓腔内的骨小梁吸收,伴微小囊肿形成.髁突关节软骨、骨组织及滑膜出现早期骨关节炎样改变.结论 兔关节盘摘除术后早期,关节区组织表现为骨关节炎样改变,不是适应性改变.     

骨皮质切开术对大鼠牙移动影响的实验研究

目的建立骨皮质切开术正畸牙移动实验动物模型,观察移动速率和对牙周组织改建的影响。方法 75只SD大鼠,实验组35只行上颌双侧中切牙骨皮质切开术,1周后与对照组35只同时进行正畸加力,空白对照组5只不进行加力。按不同加力时间处死,测量牙齿移动的距离,观察组织学改变。结果实验组2周时牙齿移动距离(3.471±0.359)mm,对照组为(1.247±0.198)mm,具有显著性差异;HE染色结果表明,实验组牙周膜内玻璃样变组织的形成相对于对照组来说,范围缩小,时间缩短。结论骨皮质切开术能够加速正畸牙移动。     

肝素抗涎腺腺样囊性癌细胞裸鼠肺血管粘附研究

目的　通过观察肝素对人涎腺腺样囊性癌肺高转移细胞株 (ACC -M )细胞在裸鼠肺、肝组织的粘附影响 ,证实其对腺样囊性癌肺高转移细胞肺粘附的抑制作用。方法　裸鼠腹腔注射肝素后 ,经尾静脉注入氚标胸腺嘧啶核苷 (3 HTdR)标记的ACC -M细胞 (3 H -ACC -M ) ;分别检测细胞注射后 2h、6h、18h肺、肝组织单位重量的每分钟同位素脉冲数 (CPM )。结果　单位重量肺组织每分钟放射性计数CPM值 :空白对照组 2h、6h、18h分别为 1875 .75± 11.5 3、164 4.5 0± 2 7.2 0和 14 66.5 0± 12 5 .95 ;2 0 0单位肝素组 2h、6h、18h分别为 912 .0 0± 118.5 5、918.0 0± 44 .0 6和 766.60± 77.66。同一时间肝素组肺组织CPM值明显低于对照组 ,有显著性差异 (P <0 .0 0 1)。单位重量肝组织CPM值在空白对照组和肝素组的相同时间点无显著性差异 (P >0 .0 5 )。结论　肝素腹腔注射后可明显降低肺组织内ACC -M细胞对血管内皮细胞粘附数量 ,但对相同时间肝组织内ACC -M细胞粘附数量无显著影响 ,提示肝素具有抑制腺样囊性癌肺转移的作用     

软骨膜中细胞初级纤毛消融对生长板发育的作用研究

目的:观察软骨膜中细胞初级纤毛消融对长骨生长板发育的影响.方法:雄性4周龄野生型小鼠20只,随机分为对照组和实验组,每组10只.实验组按照0.01 mL/g体质量于小鼠股骨生长板外局部注射10 mg/mL水合氯醛(CH),对照组于相同部位注射等量生理盐水,每2 d注射一次,连续注射4周后处死小鼠.Micro-CT扫描分...     

Er:YAG激光备洞对牙髓P物质表达的影响

目的探讨Er:YAG激光备洞对牙髓的生物学效应。方法雄性SD大鼠66只随机分为实验组与对照组,实验组大鼠左上第一磨牙用高速牙钻备洞,右上第一磨牙用Er:YAG激光备洞,对照组大鼠磨牙不作任何处理。于不同时段分批处死大鼠,取标本做HE染色进行牙髓组织病理学观察,免疫组化法观察P物质(substance P,SP)的表达情况。结果 Er:YAG激光备洞后牙髓组织病理学改变及P物质的表达改变恢复正常要快于高速牙钻备洞。结论 Er:YAG激光备洞对牙髓的刺激小。     

口腔扁平苔藓与肝病关系的病例对照研究

目的：探讨口腔扁平苔藓与慢性肝病的关系。方法：回顾性分析４７６例口腔扁平苔藓的肝病、肝功能异常发生情况，并与年龄、性别配对的４７６例其它口腔粘膜病及１７１１名健康人比较。结果：口腔扁平苔藓患者慢性肝病发生率６５％，１项肝功能异常发生率为２３．９％，二项或二项以上为５．３％。上述指标均较疾病对照组和正常对照组为高（Ｐ＜０．０１），其中尤以反映间质反应的指标如血清蛋白、ＴＴＴ、ＺｎＴＴ的异常改变较为明显。结论：口腔扁平苔藓与肝病有一定的关系，其所伴发的肝病以慢性肝病疾患为主。     

Relationship between periodontal disease and cirrhosis of the liver in humans

Abstract The periodontal condition of a well-described group of cirrhotic patients was compared with that of a control group, matched forage, sex ratio and socio-economic background, expressed as years of education. The test group comprised 30 cirrhotic patients, 35–64 years of age. The diagnosis cirrhosis of the liver was confirmed histologically, and no other systemic diseases were present. The material was grouped according to age, 35–44, 45–54 and 55–64 years of age. The examination included determination of tooth loss, a plaque index, a gingival index, retentive calculus, retentive decay and fillings, and loss of attachment. The amount of plaque was equal in test and control groups, whereas the cirrhotics had a higher degree of severity of gingival inflammation as well as a greater amount of subgingival calculus than the controls. Test and control groups exhibited no significant difference as regards loss of attachment and tooth loss, and similar correlations between loss of-attachment and age were demonstrated in the two groups. Patients suffering from cirrhosis for more than 3 years showed significantly greater loss, of attachment, as well as more plaque and calculus compared with those with a disease duration of less than 3 years. It is suggested that this aggravation of the periodontal condition is related to increasing neglect of the teeth, as the cirrhotic condition aggravates.     

Assessment and management of oral health in liver transplant candidates

Liver transplantation has become a standard treatment for end-stage liver disease and the number of recipients has grown rapidly in the last few years. Dental care during pre-transplant workup is important to reduce potential sources of infection in the drug-induced immunosuppression phase of liver transplantation.

Objectives

The objectives of this study were to document the prevalence of oral abnormalities in patients on a liver transplant waiting list presenting to an urban dental school clinic, discuss the appropriate dental treatment according their systemic conditions and compare their oral manifestations with those of healthy individuals.

Material and Methods

A pilot study was conducted involving 16 end-stage liver disease individuals (study group- SG) attending the Special Care Dentistry Center of the University of São Paulo and 16 control individuals (control group- CG) with no liver diseases, receiving dental care at the Dental School of the University of São Paulo. These individuals were assessed for their dental status (presence of oral disease or abnormalities), coagulation status, and dental treatment indications.

Results

The patients from SG exhibited a greater incidence of oral manifestations compared with CG (p=0.0327) and were diagnosed with at least one oral disease or condition that required treatment. Coagulation abnormalities reflecting an increased risk of bleeding were found in 93.75% of the patients. However, no bleeding complications occurred after dental treatment.

Conclusions

The patients with chronic liver diseases evaluated in this study exhibited a higher incidence of oral manifestations compared with the control group and had at least one oral disease or abnormality which required dental treatment prior to liver transplantation. Careful oral examination and evaluation of the patient, including laboratory tests, will ensure correct oral preparation and control of oral disease prior to liver transplantation.     

槟榔碱对口腔癌患者及小鼠肝脏功能影响的初步研究

目的:通过比较有、无槟榔咀嚼史的口腔癌患者肝功能指标变化,并与槟榔碱诱导小鼠口腔黏膜下纤维化模型的肝脏损伤比较,探讨槟榔碱对肝脏功能的影响。方法:比较有、无咀嚼槟榔史各50名口腔癌男性患者谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、总胆红素(TBIL)肝功能指标差异;将45只小鼠,随机分成对照组、停药组、不停药组,每组15只。配制1000mg/L槟榔碱溶液分别持续自由喂养停药组、不停药组2周后,停药组改用实验室灭菌水自由喂养2周,不停药组继续槟榔碱溶液喂养2周;对照组用实验室灭菌水自由喂养4周。检测各组小鼠不同时间段上述肝功能指标变化情况,并采用苏木素-伊红(HE)染色观察小鼠肝脏组织学改变。结果:与无咀嚼槟榔史患者相比,咀嚼槟榔的肝功能指标AST、TBIL显著升高(P<0.05),ALT、ALP无明显变化;与对照组小鼠相比,停药组和不停组随用药时间增长,ALT、AST、ALP显著升高(P<0.05),而停药组停药2周后ALT、AST、ALP显著下降(P<0.05),TBIL无统计学差异;HE染色显示肝脏均出现损伤,但不停药组小鼠损伤更为明显。结论:槟榔的成分对肝脏有损伤,其作用的有效成分可能是槟榔碱。     

MicroRNA expression profiling of the developing murine molar tooth germ and the developing murine submandibular salivary gland

Using microarrays, miRNA expression profiles have been established at selected times during development (E15.5, P0 and P5) of the murine first molar mandibular tooth germ and the right submandibular salivary gland (E15.5, P0, P5 and P25). Microarray data was validated using real-time PCR, also facilitating RT-PCR profiling of nine selected miRNAs. In general, good agreement between microarray data and real-time PCR data was found. Further, miRNA expression profiles of foetal and adult liver were also investigated, and found to agree with published data. In tooth germ and salivary gland up to 88 different miRNAs were detected. In all tissues examined miRNA expression was highly dynamic; miRNA profiles changing extensively with time of development. Additionally, the expression of some miRNAs was tissue-specific. Bioinformatic analysis of clusters of miRNAs was attempted using the miRGate software, the results suggesting miRNAs to be involved in the regulation of essential developmental processes, e.g., epithelical cell proliferation, mesodermal cell fate determination and salivary gland morphogenesis.     

肝移植术后口腔粘膜病变的诊治

目的：探讨肝移植术后口腔粘膜病变的诊治。方法：通过所经治的１０例临床活体肝部分移植术患儿，报告了口腔粘膜病变的特点。结果：有４例患儿出现了口腔粘膜病变，分别为单纯疱疹１例，口腔真菌感染１例，原因不清的复合性病变２例，全部病例都经治而愈。结论：肝移植术后口腔粘膜病变主要由病毒、真菌、药物或营养失衡所致，其诊治各有特点，需区别对待