尤瑞克林联合依达拉奉治疗急性脑梗死疗效及血清hs-CRP和VEGF水平变化

目的:观察尤瑞克林联合依达拉奉对急性脑梗死的临床疗效及对血清超敏C反应蛋白(hs-CRP)、血管内皮生长因子(VEGF)的影响。法:121例病人,随机分为观察组(58例)和对照组(63例),对照组给予阿斯匹林、依达拉奉静滴;观察组在对照组基础上给予尤瑞克林静滴。疗程14天。并在确诊后24 h和治疗14天测定两组血清hs-CRP、VEGF水平。结果:观察组治疗14天后, hs-CRP下降幅度、VEGF上升幅度均高于对照组(P0.05);观察组临床疗效亦优于对照组(P0.05)。结论:尤瑞克林联合依达拉奉可能通过提高VEGF及降低hs-CRP水平治疗急性脑梗死。     

依达拉奉联合马来酸桂哌齐特治疗急性脑梗死的疗效观察

目的：观察依达拉奉联合马来酸桂哌齐特治疗急性脑梗死(ACI)的临床疗效和安全性。方法采用随机对照试验,将发病72h内的ACI患者随机分为两组。治疗组(28例)应用依达拉奉30mg静脉滴注,2次/d;马来酸桂哌齐特320mg静脉滴注,1次/d,两药均使用14d。对照组(25例)应用丹红40ml代替依达拉奉及马来酸桂哌齐特,基础治疗两组相同。治疗前后进行神经功能缺损(ESS)评分。结果14d后治疗组神经功能缺损评分明显低于对照组,治疗总有效率明显高于对照组(<0.05),且无明显不良反应。结论依达拉奉联合马来酸桂哌齐特治疗急性脑梗死疗效确切,安全可靠。     

依达拉奉联合高压氧降低脑梗死大鼠脑组织环氧合酶2及β淀粉样肽的表达

目的探讨依达拉奉联合高压氧对脑梗死(MCAQ)模型大鼠脑组织环氧合酶2(COX-2)及β淀粉样肽(Aβ)表达的影响。方法成年健康SD大鼠60只,建立MCAQ模型。随机分为4组,每组15只。脑梗死组(尾静脉注射0.1 mol/L PBS)、依达拉奉组(静脉滴注依达拉奉30 mg,bid)、高压氧组(0.22 MPa,80 min高压氧治疗,qd)、联合组(依达拉奉治疗同时,联合高压氧治疗),共处理21 d。治疗前及治疗后对各组大鼠进行神经功能学评分,观察各组疗效;应用反转录PCR、Western blot法检测脑梗死模型大鼠脑组织内COX-2、Aβ的mRNA及蛋白水平;免疫组织化学染色观察脑组织胶质纤维酸性蛋白(GFAP)的表达;原位末端转移酶标记技术(TUNEL)测定细胞凋亡的情况。结果依达拉奉组、高压氧组大鼠的神经功能缺损评分明显低于对照组,联合组明显低于依达拉奉组及高压氧组;依达拉奉组、高压氧组COX-2、Aβ的mRNA及蛋白水平明显低于脑梗死组,联合组明显低于依达拉奉组及高压氧组;依达拉奉组、高压氧组GFAP阳性细胞数目明显少于脑梗死组,联合组阳性细胞数又明显少于依达拉奉组及高压氧组;联合组的凋亡细胞数明显少于依达拉奉组、高压氧组及脑梗死组。结论依达拉奉联合高压氧能降低脑梗死大鼠脑组织中COX-2及Aβ的表达。     

早期应用依达拉奉对大面积脑梗死患者血清神经元特异性烯醇酶和C反应蛋白水平及病死率的影响

目的 观察大面积脑梗死患者早期应用依达拉奉对病死率以及血清NSE和CRP水平的影响.方法 90例(发病48 h内入院)急性大面积脑梗死患者,随机分为治疗组(依达拉奉 常规治疗)和对照组(常规治疗),治疗前、后进行NIHSS评分,统计病死率和检测血清NSE和CRP水平,并对两组间和治疗前、后的数据比较.结果 治疗组的病死率显著低于对照组,治疗后第7、14、21 d的NIHSS评分无显著差异;与对照组相比,治疗后第7、14 d血清NSE水平差异显著,第21 d无显著差异;第7 d血清CRP水平相差显著,第14、21 d则无显著差异;与治疗前相比,治疗组治疗后第7、14、21 d血清NSE和CRP水平均有显著下降,对照组第14、21 d有显著下降.血清NSE和CRP水平呈正相关变化.结论 早期应用依达拉奉可以显著降低患者血清NSE和CRP水平,减轻脑组织损伤,并显著降低大面积脑梗死患者病死率.     

依达拉奉对外伤性脑内出血患者血浆ET-1和CGRP影响的临床探讨

目的：探讨外伤性脑内出血患者血浆ET-1和CGRP变化以及依达拉奉治疗对血浆ET-1和CGRP的影响。方法：80例外伤性脑内出血患者随机分为依达拉奉组与常规治疗组,另取30例健康人作为对照组。用放射免疫分析测定外伤性脑内出血患者依达拉奉治疗前后血浆ET-1、CGRP含量。结果：依达拉奉组和常规治疗组治疗前血浆ET-1显著增高,治疗后明显下降,治疗后血浆ET-1降低常规治疗组明显少于依达拉奉组（P〈0.01）;依达拉奉组和常规治疗组治疗前血浆CGRP显著降低,治疗后明显升高,治疗后血浆CGRP增加,依达拉奉组明显高于常规治疗组（P〈0.01）。结论：外伤性脑内出血患者血浆ET-1水平显著升高,CGRP水平显著降低;早期应用依达拉奉可显著降低ET-1和提高CGRP水平,初步研究显示,依达拉奉具有脑保护作用。伤后早期应用依达拉奉可改善病人预后。     

依达拉奉治疗急性脑梗死临床疗效评价

目的观察依达拉奉治疗急性脑梗死的疗效。方法急性脑梗死120例随机分为治疗组(依达拉奉加常规治疗),对照组(常规治疗)两组,治疗组60例,对照组60例。观察治疗后神经功能改善情况并进行疗效评定。结果治疗组治疗后各时期ESS及ADL评分均显著高于对照组(<0.05),14d、21d及30d的ESS及ADL评分显著高于对照组(<0.05)。结论依达拉奉能显著提高急性脑梗死的疗效。     

奥扎格雷联合依达拉奉治疗急性脑梗死的效果

目的 观察奥扎格雷联合依达拉奉治疗急性脑梗死的效果。方法 选择2018年5月~2019年5月在我院诊治的急性脑梗死患者106例,采用随机数字表法分为对照组和观察组,各53例。对照组采用奥扎格雷治疗,观察组在对照组基础上联合依达拉奉治疗,比较两组临床治疗总有效率、治疗前后神经功能缺损评分（NIHSS）和日常生活能力评分（Barthel）、临床不良反应。结果 观察组治疗总有效率为94.33%,高于对照组的81.13%,差异有统计学意义（P＜0.05）;治疗后两组NIHSS评分均低于治疗前,且观察组低于对照组,差异有统计学意义（P＜0.05）;治疗后两组Barthel评分均高于治疗前,且观察组高于对照组,差异有统计学意义（P＜0.05）;观察组临床不良反应率为5.66%,与对照组的7.54%比较,差异无统计学意义（P＞0.05）。结论 奥扎格雷联合依达拉奉治疗急性脑梗死效果确切,有助于改善神经功能,提高患者日常生活能力,同时临床不良反应小,联合用药方案安全可行,值得临床应用。     

依达拉奉联用高氧液治疗急性脑梗死疗效观察

目的 观察依达拉奉联用高氧液治疗急性脑梗死的疗效并进行分析和评价。方法 对30例脑梗死患者在常规治疗的基础上加用依达拉奉和高氧液治疗，并与对照组40例比较。结果 应用依达拉奉与高氧液治疗组临床疗效较对照组有显著提高。结论 依达拉奉联用高氧液治疗急性脑梗死能明显提高显效率及改善神经功能。     

依达拉奉联合奥扎格雷钠治疗急性脑梗死的疗效观察

目的观察依达拉奉联合奥扎格雷钠治疗急性脑梗死的疗效。方法将88例急性脑梗死患者随机分为治疗组44例,采用依达拉奉与奥扎格雷钠联合治疗;对照组44例使用奥扎格雷钠治疗。比较两组治疗效果。结果治疗级显交往率63.6%,总有效率95.5%,显著高于对照组的显效率40.9%,总有效率75.0%,P<0.05,其差异具有统计学意义。结论依达拉奉联合奥扎格雷钠治疗急性脑梗死效果好且安全。     

依达拉奉、吡拉西坦联合治疗对大面积脑梗塞神经功能恢复作用及安全性观察

目的:观察大面积脑梗塞患者经依达拉奉、吡拉西坦联合治疗后神经功能的恢复效果及安全性.方法:选取2019年3月至2021年9月期间于我院就诊的97例大面积脑梗塞患者为此次研究对象,根据治疗药物的不同分为研究组(n=50)和对照组(n=47),其中对照组患者采用依达拉奉(依达拉奉30 mg与0.9％氯化钠溶液100 mL混合后静脉滴入,Bid)治疗,而研究组患者采用依达拉奉、吡拉西坦(依达拉奉治疗方法与对照组相似,在将吡拉西坦2 g与0.9氯化钠溶液100 mL混合后静脉滴入,Bid)联合治疗.治疗2 w后比较两组临床疗效,并采用美国国卫院卒中量表神经缺损功能评分量表(National institute of Health Stroke Scale,NIHSS)评价神经功能,同时观察不良反应发生情况.结果:研究组临床总有效率明显高于对照组(P<0.05);与治疗前相比,两组NIHSS评分均明显降低(P<0.05),其中研究组更为显著(P<0.05);但两组患者治疗后不良反应未见统计学差异(P>0.05).结论:依达拉奉、吡拉西坦联合治疗大面积脑梗塞临床疗效显著,可有效促进神经功能恢复,且安全性较好.     

局部注射辛伐他汀对骨质疏松大鼠股骨髁骨小梁的改建效应

背景：局部注射具有成骨作用的辛伐他汀,可显著增加骨质疏松大鼠股骨颈及股骨髁部的骨密度及力学强度,分析局部注射辛伐他汀对股骨髁骨小梁的影响。 目的：进一步研究骨质疏松大鼠股骨内局部注射辛伐他汀对股骨髁骨小梁的影响。为将辛伐他汀应用于临床骨质疏松局部治疗提供实验基础。 方法：18只雌性SD大鼠双侧卵巢切除后3个月,制备大鼠骨质疏松模型。实验大鼠随机数字表法均分为3组,分别在实验大鼠的右侧股骨髓腔内单次注射辛伐他汀溶液5 mg、10 mg,对照组单纯注射空白载体。分别在注射后1个月处死大鼠并取材。Micro-CT扫描并定量分析骨组织形态变化。 结果与结论：给药后1个月,Micro-CT扫描结果显示,辛伐他汀治疗组的骨微结构参数如骨皮质厚度、骨小梁密度及连接率明显优于对照组。说明疏松骨骼单次注射小剂量辛伐他汀可显著促进股骨髁部骨小梁改建,改善骨骼微结构,可为强化局部、防治骨质疏松骨折的新选择进一步提供实验基础。     

Assessment of the efficacy of treatment of hemangiomas

Assessment of the aftereffects of cryoexposure and ultrahigh-frequency cryoexposure on hemangioma tissue of various types, cavernous and squamous, showed a higher cryogenic effect in hemangiomatous tissue preexposed to ultrahigh-frequency waves. A quantitative criterion is proposed for assessing the efficacy of the studied methods of exposure. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 6, pp. 669–672, June, 1995     

Level of functioning of siblings and parents of probands of varying degrees of retardation

A further analysis of already published data supports the position that retardates of low ability level less frequently have retarded siblings, retarded parents, and parents low in occupational level than do retardates higher in ability level. The analysis supports the position that there are two types of retarded individuals, persons retarded as a result of gene or chromosomal anomalies, brain injury, etc., who more frequently occur in the lower-level retardate group, and persons whose retardation represents polygenic segregation, who more frequently occur in the higher-level group.     

侧脑室注射链脲佐菌素对大鼠海马神经元突触的影响

为观察链脲佐菌素(streptozotocin,STZ)双侧侧脑室注射对大鼠海马神经元突触的影响,本研究将Wistar大鼠随机分为对照组和模型组,模型组大鼠分别于第1、3d双侧侧脑室重复注射STZ3mg/kg,对照组以人工脑脊液代替STZ。21d后,取大鼠海马,免疫组织化学染色及Western blotting方法观察突触素、活性调节细胞骨架相关蛋白(activity-regulated cytoskeletal-associatedprotein,Arc)的表达;电镜观察海马CA1区神经元突触超微结构的改变。结果显示:与对照组相比,模型组大鼠海马内突触素蛋白表达显著减少,而Arc蛋白表达显著增多;模型组海马CA1区神经毡内突触结构异常,突触小泡聚集增多。以上结果提示:脑室注射STZ可影响大鼠海马突触相关蛋白的表达,引起突触超微结构异常,干扰了神经元突触信号的传导。     

Effect of conditions of culture on quantitative assessment of viability of living tissues

The viability of tissue explants of mouse kidneys, estimated quantitatively by growth in plasma-free culture, was shown to depend on several factors connected with the conditions of culture. The effect of methods of treatment of the serum, pH of the medium, and the character of distribution of the tissue explant during culture, the size of the explants, and the firmness of attachment of the tissue fragments to the substrate on this index was demonstrated.Problem Laboratory, Arkhangel'sk Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR A. P. Avtsyn.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 86, No. 11, pp. 629–631, November, 1978.     

社区精神分裂症病人照料者的生活质量研究

通过对９３名精神分裂症患者的家庭照料者与１００名对照者比较研究表明，照料者的躯体功能、心理功能、社会功能与经济状况都显著差于对照者。照料者的生活质量与被照料者的病期、病情严重度、整体功能、药物副作用有关；同时，照料者身份为患者母亲者其生活质量受影响最大。     

Evaluation of fungicidal activity of extracellular filtrates of cyanobacteria--possible role of hydrolytic enzymes

A set of seventy axenised and unicyanobacterial isolates belonging to the genus Anabaena were evaluated for biocidal activity against a set of phytopathogenic fungi. Among them, 35 Anabaena strains showed zone of inhibition against one or more fungi. The extracellular filtrates from 4 and 8 weeks old cultures of these Anabaena strains were further evaluated in terms of hydrolytic enzymes, proteins and IAA employing standard methods. Significant differences were also observed among the strains in terms of their FPase, chitosanase and xylanase activity, while low and relatively similar values of CMCase, cellobiase and protease activity were recorded in the strains analyzed. IAA production was also observed in all the strains. Comparative evaluation of activity of hydrolytic enzymes and antifungal activity revealed that such enzymes may contribute to the fungicidal activity of the cyanobacterial strains, besides other bioactive compounds, including IAA, which are established promising traits for biocontrol agents. This study is a first time report on the production of hydrolytic enzymes by these oxygenic photosynthetic prokaryotes, which can be potential candidates for the development of biocontrol agent(s) against selected phytopathogenic fungi.     

Effect of ultralow doses of thyroliberin on microviscosity of lipid components of biological membranes

Effects of the regulatory peptide thyroliberin on microviscosity of lipid components of endoplasmic reticulum biological membranes in mouse hepatocytes were studied by electron paramagnetic resonance. Thyroliberin in a concentration of 10⁻³–10⁻¹⁸ M decreased microviscosity of surface layers of membrane lipids. This decrease was the most pronounced (30%) under effects of 10⁻¹⁰ and 10⁻¹⁶ M thyroliberin. Physiological effects of thyroliberin corresponded to its influence on the membrane structure. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 129, No. 1, pp. 38–40, January, 2000     

Effect of route of antitoxin administration on efficacy of treatment of experimental tetanus

The efficacy of treatment with Diaform-3 antitoxin, given by either intracisternal or intralumbar injection in a dose of 400 i.u./kg, was compared in experiments on 100 rabbits with ascending, hematogenous, and cerebral tetanus caused by injection of 1 c.l.d. of tetanus toxin. Intracisternal injection of the antitoxin was found to be three times more effective than intralumbar (the proportion of animals cured was 31.4 and 10.2% respectively). The latter was effective only in animals with ascending tetanus, evidently because the portal of entry of the toxin into the CNS was along the peripheral nerves of the hind limbs.Central Research Laboratory, L'vov Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR A. M. Chernukh.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 86, No. 8, pp. 212–213, August, 1978.