川西獐牙菜甙类成分

对川西獐牙菜(Swertia mussotii Franch.)的水溶性成分进行了研究。应用层析方法,分离得到裂环烯醚萜甙,黄酮甙,(口山)酮甙,3类8种单体成分(Ⅰ—Ⅷ)。除先前报道过的芒果甙外(Ⅱ),又分离和鉴定了苦龙甙(Ⅲ),当药黄素(Ⅷ),8-O-β-D-吡喃葡萄糖-1,3,5-三羟基(口山)酮(Ⅶ),8-O-[β-D-吡喃木糖-(1→σ)-β-D-吡喃葡萄糖]-1,7-二羟基-3-甲氧基(口山)酮(Ⅵ)4种已知天然化合物。应用化学和光谱分析方法,测定另外3种新(口山)酮甙的结构为:7-O-[α-L-吡喃鼠李糖-(1→2)-β-D-吡喃木糖]-1,8-二羟基-3-甲氧基咄酮(Ⅰ),7-O-β-D-吡喃木糖-1,8-二羟基-3-甲氧基(口山)酮(Ⅳ),3-O-β-D-吡喃葡萄糖-1,8-二羟基-5-甲氧基(口山)酮(Ⅴ)。芒果甙,苦龙甙和7-O-[α-L-吡喃鼠李糖-(1→2)-β-D-吡喃木糖]-1,8-二羟基-3-甲氧基(口山)酮为川西獐牙菜主要甙类成分。有兴趣的是在已发现的龙胆科植物(口山)酮糖甙中,未见(口山)酮木糖甙,(口山)酮鼠李糖-木糖甙的报道。     

藏药黑边假龙胆的化学研究

龙胆科植物黑边假龙胆（Ｇｅｎｔｉａｎｅｌｌａａｚｕｒｅａ（Ｂｕｎｇｅ）Ｈｏｌｕｂ）是治疗肝胆湿热的１种藏药,从西藏日喀则产的全草中分离得到９个化合物,其中１个Ｃ一甙黄酮,异荭草素（ｉｓｏｏｒｉｅｎｔｉｎ）;１个山酮甙,獐牙菜酚甙（ｓｗｅｒｔｉａｎｏｌｉｎ）;１个木质体甙,橄榄脂素４一Ｏ一β－Ｄ－葡萄糖甙（ｏｌｉｖｉｌ４一Ｏ一β一ｇｌｕｃｏｓｉｄｅ）;３个环烯醚萜甙,龙胆苦甙（ｇｅｎｔｉｏｐｉｃｒｏｓｉｄｅ）,獐牙菜甙（ｓｗｅｒｔｉａｍａｒｉｎ）和马钱子酸（ｌｏｇａｎｉｃａｃｉｄ）;１个三萜,齐敦果酸（ｌｏｅａｎｏｌｉｃａｃｉｄ）１个甾醇,β谷甾醇葡萄糖甙（ｄａｕｃｏｓｔｅｒｏｌ）以及１个长链脂肪醇,三十烷醇（ｎ一１一ｔｒｉａｃｏｎｔａｎｏｌ）。它们的结构通过光谱和化学的方法得到了鉴定。上述结果,从次生代谢产物的角度为假龙胆属在系统分类上与龙胆属和獐牙菜属有着密切的联系提供了旁证。     

枸骨的化学成分

从枸骨(Ilex cornuta Lindl,ex paxt.)中分离得到20个化合物,鉴定了16个,分别为：枸骨甙1(Gougusidel,V)即坡摸酸3-β-O-O-L吡喃阿拉伯糖甙;枸骨甙2(Gouguside 2,Ⅵ)即3-β-O-D-吡喃葡萄糖基坡摸酸-β-28-O-D吡喃葡萄糖酯;枸骨甙3(Gouguside 3,Ⅶ)即3-β-O-(β-D-吡喃葡萄糖基)-α-L吡喃葡萄糖基坡摸酸-β-28-O-D-吡喃葡萄糖酯的类似物;枸骨甙4(Gouguside 5,ⅩⅦ)即3-β-O-(β-D-吡喃葡萄糖基)-α-L-吡喃葡萄糖基坡摸酸-β-28-O-D-吡喃葡萄糖酯;枸骨甙5(Gouguside 5,ⅩⅧ)即坡摸酸3-β-O-α-L-2’-乙酰氧基吡喃阿拉伯糖基-28-O-β-D-吡喃葡萄糖酯,枸骨甙6(Gouguside 6,ⅩⅪ)即3-β-O-(β-D-吡喃葡萄糖基)-α-L-4-乙酰氧基吡喃阿拉伯糖基坡摸酸-β-28-O-D-吡喃葡萄糖酯;枸骨甙7(Gouguside 7,ⅩⅩ)即3-β-O-α-L-吡喃阿拉伯糖基-28-O-β-D-吡喃葡萄糖酯;胡萝卜甙(daucostorol Ⅷ);2,4-二羟基苯甲酸(2,4-dihydroxybenzoicacid I);3,4-二羟基桂皮酸(3,4-dihyroxycinnamunic acid Ⅱ,Ⅳ);长链脂肪酸或醇5个(Longchain fatty acid Ⅲ,Ⅵ,Ⅶ,Ⅷ,ⅩⅣ)。在鉴定的16个化合物中,枸骨甙1、枸骨甙2、枸骨甙6和枸骨甙7为首次从枸骨中分离得到,枸骨甙3和枸骨甙4为新化合物。     

抱茎獐牙菜的(口山)酮甙和黄酮甙

从龙胆科植物抱茎獐牙菜(Swertia franchtiana H.smith)中分得3种(口山)酮甙,1种黄酮甙和1种三萜酸,通过光谱和化学等方法,分别鉴定为当药醇甙,当药黄素,芒果甙,1-O-[β-D-吡喃木糖-(1-6)-β-D-吡喃葡萄糖]-3,5-二甲氧基咄酮(XI)和齐墩果酸,其中晶 XI 为一新化合物。     

药囊花茎叶的化学成分研究

药囊花（Cyphotheca montana Diels）茎叶的乙醇提取物具有抗菌活性,进一步的活性追踪表明活性成分在乙酸乙酯和正丁醇萃取物中,我们从这两种萃取物中共分离鉴定出9个化合物,其结构通过波谱分析等分别鉴定为：槲皮素-3-O-α-L-鼠李糖甙（1）,杨梅素-3-O-α-L鼠李糖甙（2）,山萘酚-3-（2″,6″-二-O-（E）-p-羟基苯丙烯酰-β-D-吡喃葡萄糖甙（3）,3,3′-O-二甲基逆没食子酸-4-O-β-D-吡喃葡萄糖甙（4）,槲皮素（5）,2α,3β,19α,23-四羧基乌苏-12-烯-28-酸-28-0-β-D吡喃葡萄糖甙（6）,乌苏酸（7）,β-谷甾醇（8）和胡萝卜甙（9）,这些化合物均为首次从我国该特有单种属植物中分离得到。     

天山棱子芹化学成分的研究

从天山棱子芹中首次分离得到15个已知化合物,通过NMR、MS及IR等波谱数据,分别鉴定为6,7-二羟基香豆素(1),( )-marmesin(2),marmesinin(3),5,7,4'-三羟基黄酮(4),莰非醇3-O-α-L-吡喃鼠李糖甙(5),藤黄菌素3'-O-β-D-吡喃葡萄糖甙(6),(R)-6-hydroxy-3-(2-hydroxypropan-2-yl)-6-methylcyclohex-2-enone(7),4-羟基苯甲酸(8),3-甲氧基4羟基苯甲酸(9),3-甲氧基-4,5-亚甲二氧基苯甲酸(10),丁香酸甲酯(11),丁香酸甲酯4-O-β-D-吡喃葡萄糖甙(12),姜油酮4’-O-β-D-吡喃葡萄糖甙(13),2-(4-羟基苯基)-乙醇(14)和正二十八醇(15)。其中化合物7为一新的天然产物。     

茉莉花的化学成分

从药用植物茉莉花(Jasminum sambac(L．)Ait．)花蕾中分离到9个化合物,通过波谱分析并与已知化合物数据对照,分别鉴定为：苄基-O-β-D-葡萄吡喃糖甙(1),苄基-O-β-D-木吡喃糖基(1→6)-β-D-葡萄吡喃糖甙(2),tetraol(3),molihuaoside D(4),sarnhcoside A(5),sambacoside E(6),芦丁(rutin)(7),山奈酚-3-O-α-L鼠李吡喃糖基(1-2)[α-L鼠李吡喃糖基(1-6)]-β-D半乳吡喃糖甙(8),斛皮素-3-O-α-L鼠李吡喃糖基(1→2)[α-L鼠李吡喃糖基(1→6)]-β-D-半乳吡喃糖甙(9)。     

丝瓜中一个新脑甙脂类化合物

在继续研究鲜丝瓜果实成分时,又分离出3个化台物,其一(L-I)为一新脑甙脂,mp、140°～142℃,分子式:C_(39)H_(73)O_9N,它的结构经理化及光谱(IR、MS、HNMR、~(13)CNMR)数据确定为N-[D-2~1羟基十四烷酰基]-1-O-[β-D-吡喃葡萄糖)-D-赤式-十九鞘氨-4(E),8(E)-二烯醇,命名为Lucyobroside。另2个化合物(L-Ⅱ与L-Ⅲ)为已知五环三萜皂甙,即3-O-[β-D-吡喃葡萄糖)-常春藤甙元-28-O-β-D-吡喃葡萄糖甙(Lucyoside E);3-O-β-D-吡喃葡萄糖]-丝石竹甙元-28-O-β-D-吡喃葡萄糖甙(Lucyoside F),此两皂甙均系首次从我国产丝瓜中分得。     

15种獐牙菜属植物中主要药用成分的高效液相色谱测定

对青藏高原和云贵高原的15种獐牙菜属植物进行了3种苦味苷,即獐牙菜苦苷(swertiamarin)、龙胆苦苷(gentiopicroside)、苦龙苷(amarogentin)、一种黄酮苷-当药黄素(swertisin)、及5种口山酮苷-芒果苷(mangiferin)、当药醇苷(swertianolin)、7-O-[a-L-吡喃鼠李糖-(1→2)-β-D-吡喃木糖]-1,8-二羟基-3-甲氧基口山酮(7-O-[a-L-rhamnopyranosyl-(1-2)-β-D-xylopyranosyl]-1,8-dihydroxy-3-methoxyxanthone)、7-O-β-D-吡喃木糖-1,8-二羟基-3-甲氧基口山酮(7-O-β-D-xylopyranosyl-1,8-dihydroxy-3-methoxyxanthone)、3-O-β-D-吡喃葡萄糖-1,8-二羟基-5-甲氧基口山酮(3-O-β-D-glucopyranosyl-1,8-dihydroxy-5-methoxyxanth-one)等9种主要药效成分同时进行了高效液相色谱的含量测定(Kromasil C18柱,甲醇一水梯度洗脱,二级管阵列检测);并对其主要药效成分的分布进行了比较。     

珍珠菜中黄酮及Megastigmane类化学成分研究北大核心CSCD

对珍珠菜Lysimachia clethroides的化学成分进行研究。应用硅胶、Sephadex LH-20和制备型HPLC等柱色谱技术进行分离纯化,并运用现代波谱技术（ESI-MS,^1H-NMR,^13C NMR）进行结构鉴定。从珍珠菜中分离得到12个化合物,分别鉴定为山柰酚（1）、槲皮素（2）、cinchonain Ib（3）、芹菜素-6-C-β-D-吡喃木糖基-8-C-α-L-吡喃阿拉伯糖苷（4）、芹菜素-6,8-二-C-α-L-吡喃阿拉伯糖苷（5）、芹菜素-6-C-α-L-吡喃阿拉伯糖基-8-C-β-D-吡喃木糖苷（6）、芹菜素-6,8-二-C-β-D-吡喃木糖苷（7）、芹菜素-6-C-β-L-吡喃阿拉伯糖基-8-C-β-D-吡喃葡萄糖苷（8）、芹菜素-6-C-α-L-吡喃阿拉伯糖基-8-C-β-D-吡喃葡萄糖苷（9）、芹菜素-6-C-β-D-吡喃葡萄糖基-8-C-α-L-吡喃阿拉伯糖苷（10）、blumenol A（11）、（3S,5R,6R,7E,9S）-大柱香波龙-7-烯-3,5,6,9-四醇-3-O-β-D-吡喃葡萄糖苷（12）。化合物3-8和11-12为首次从该属植物中分离得到。体外活性测试结果显示化合物1-12均没有明显的细胞毒活性（IC50〉10 μM）。     

A New Secoiridoidal Glucosides from Gentiana rigescens (Gentianaceae)

A new acylated secoiridoidal glucoside, named gentiorigenoside A (1 ), was isolated from the root of Gentiana rigescens (Gentianaceae) , together with eight known compounds, gentiopicroside ( 2) , 6′- O-β- D-glucopyranosyl gentiopicroside (3) , loganic acid (4 ) , 6′- O-β- D-glucopyranosyl loganic acid ( 5), sweroside ( 6 ), 2′-( o, m-dihydoxybenzyl) -sweroside (7), swertiamarin (8) and secologanoside (9) . heir structures were elucidated on the basis of detailed spectroscopic analysis. Iridoidal glucosides 3, 5 and 9 were isolated for the first time from the title plant.     

锐尖山香圆叶中三萜类成分的研究

从锐尖山香圆(Turpinia arguta (Lindl.) Seem.)叶中分离得到了11个三萜类化合物。通过光谱分析,分别鉴定其结构为熊果酸(1), 3β,6β,23-trihydroxy-12-oleanen-28-oic acid (2), 3β,6β,23-trihydroxyurs-12-en-28-oic acid (3), 3β,6β,19α,23-tetrahydroxyurs-12-en-28-oic acid (4), 1 α, 3β,23-trihydroxy-12-oleanen-28-oic acid (5), arjunglucoside II (6), rosamultin (7), 3β-O-β-D-glucopyranoylcincholic acid (8), cinchonaglycoside C (9), mussaendoside S (10) 和3β-O-β-D-glucopyranosyl quinovic acid 28-O-β-D-glucopyranosyl ester (11)。除化合物1和6,其它化合物均为首次从山香圆叶中分离得到。     

肋果茶中的萜类化学成分研究

从肋果茶(Sladenia celastrifolia)95％乙醇提取物的乙酸乙酯部位中分离得到15个萜类化合物,经波谱学方法分别鉴定为sladeniafolin A(1),grasshopper ketone (2),(3S,5R,6S,7E,9R) -7-megastigmene-3,6,9-triol (3),hedytriol (4),(3S,5R,6R,7E,9R) -3,5,6,9-tetrahydroxy-7-megastigmene(5),1′S*,4′R*-8-(4′-hydroxy-2′,6′,6′-trimethylcyclohex-2-enyl)-6-methyloct-3E,5E,7E-trien -2-one (6),2α,3α,19α,23-tetrahydroxyurs-12-en-28-oic acid (7),2α,3β,19α,23-tetrahydroxyurs-12-en-28-oic acid(8),pomolic acid(9),3-O-acetyl pomolic acid(10),ursaldehyde (11),camarolide (12),3β-hydroxyurs-11-en-13β(28) -olide (13),3β-hydroxy -11α,12α-epoxy-urs-13β,28-olide (14)和28-0-β-D-glucopyranosyl euscaphic acid (15).以上化合物均首次从该植物中分离得到,其中1为新的C9裂环烯醚萜.     

无柄新乌檀中的配糖体

从无柄新乌檀乙醇浸膏的正丁醇部位分离得到7个已知配糖体化合物,经波谱分析为：喹诺酸-3-O-β-D-葡萄吡喃糖基(28→1)-β-D-葡萄吡喃糖酯(1),齐墩果酸-(28→1)-β-D-葡萄吡喃糖酯(2),熊果酸-(28→1)-β-D-葡萄吡喃糖酯(3),喹诺酸-3-O-β-D-葡萄吡喃糖基-(1→3)-6-去氧-β-葡萄吡喃糖苷(4),齐墩果酸-3-O-β-D-吡喃木糖基-(1→2)-β-D-葡萄吡喃糖基-28-O-β-D-葡萄吡喃糖酯(5),番木鳖甙(6),7-甲氧基-龙胆苦甙(7)。这些化合物均为首次从该属中分离得到。     

Acylated protopanaxadiol-type ginsenosides from the root of Panax ginseng

Six new protopanaxadiol-type ginsenosides, named ginsenosides Ra(4) -Ra(9) (1-6, resp.), along with 14 known dammarane-type triterpene saponins, were isolated from the root of Panax ginseng, one of the most important Chinese medicinal herbs. The structures of the new compounds were determined by spectroscopic methods, including 1D- and 2D-NMR, HR-MS, and chemical transformation as (20S)- 3-O-{β-D-6-O-[(E)-but-2-enoyl]glucopyranosyl-(1→2)-β-D-glucopyranosyl}-20-O-[β-D-xylopyranosyl-(1→4)-α-L-arabinopyranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (1), (20S)-3-O-[β-D-6-O-acetylglucopyranosyl-(1→2)-β-D-glucopyranosyl]-20-O-[β-D-xylopyranosyl-(1→4)-α-L-arabinopyranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (2), (20S)-3-O-{β-D-6-O-[(E)-but-2-enoyl]glucopyranosyl-(1→2)-β-D-glucopyranosyl}-20-O-[β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (3), (20S)-3-O-{β-D-6-O-[(E)-but-2-enoyl]glucopyranosyl-(1→2)-β-D-glucopyranosyl}-20-O-[α-L-arabinopyranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (4), (20S)-3-O-{β-D-4-O-[(E)-but-2-enoyl]glucopyranosyl-(1→2)-β-D-glucopyranosyl}-20-O-[α-L-arabinofuranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (5), (20S)-3-O-{β-D-6-O-[(E)-but-2-enoyl]glucopyranosyl-(1→2)-β-D-glucopyranosyl}-20-O-[α-L-arabinofuranosyl-(1→6)-β-D-glucopyranosyl]protopanaxadiol (6). The sugar moiety at C(3) of the aglycone of each new ginsenoside is butenoylated or acetylated.     

Oleanane-type triterpenoids from Panax stipuleanatus and their anticancer activities

One newly (1) and 10 known oleanane-type triterpenoids (2-11) were isolated from the methanol extract of Panax stipuleanatus rhizomes. Based on their spectroscopic data, these compounds were identified as spinasaponin A methyl ester (1), pesudoginsenoside RP(1) methyl ester (2), spinasaponin A 28-O-glucoside (3), pseudoginsenoside RT(1) methyl ester (4), pseudoginsenoside RT(1) (5), stipuleanoside R(2) methyl ester (6), stipuleanoside R(2) (7), araloside A methyl ester (8), 3-O-β-D-glucopyranosyl (1→3)-β-D-glucuronopyranoside-28-O-β-D-glucopyranosyl oleanolic acid methyl ester (9), 3-O-β-D-xylopyranosyl (1→2)-β-D-glucopyranosyl-28-O-β-D-glucopyranosyl oleanolic acid (10), and chikusetsusaponin IVa (11). When the cytotoxic activities of the isolated compounds were evaluated, compound 1 exhibited significant cytotoxic activity with IC(50) values of 4.44 and 0.63 μM against HL-60 (leukemia) and HCT-116 (colon cancer) cell lines, respectively. Compound 2 showed potent cytotoxicity with an IC(50) of 6.50 μM against HCT-116, whereas it was less cytotoxic against HL-60 (IC(50)=41.45 μM). After HL-60 and HCT-116 were treated with compounds 1 and 2, increased production of apoptotic bodies was observed. Furthermore, compounds 1 and 2 in HCT-116 cells activated intrinsic and extrinsic apoptosis pathways by upregulating DR-5 and Bax, downregulating Bcl-2, activating caspase-9, and cleaving poly-ADP-ribose polymerase (PARP). We also observed the activation of ERK1/2 MAPK by both compounds in the HCT-116 cells. Together, compounds 1 and 2 might induce intrinsic and extrinsic apoptosis pathways through the activation of the ERK1/2 MAPK pathway in HCT-116 colon cancer cells. Structure-activity relationship analysis indicated that a carboxyl group at position-28 is potentially responsible for the cytotoxic effects.     

鬼针草中一个新的查耳酮甙

从鬼针草BidenspilosaL .地上部分的丙酮提取物中 ,分离鉴定了 1 8个化合物 ,其中包括一个新的查耳酮甙类化合物 (α,3,2′,4′ tetrahydroxy 2′ O β D glucopyranosylchalcone,2 )。其它化合物分别鉴定为butein (1 ) ,okanin 4 methylether 3′ O β glucoside (3) ,sulfuretin (4) ,6 ,7,3′,4′ tetrahydroxyaurone (5) ,海生菊苷 (maritimein ,6) ,(Z ) 6 O (6″ acetyl β D glucopyr anosyl) 6 ,7,3′,4′ tetrahydroxy aurone (7) ,(Z ) 6 O (4″,6″ diacetyl β D glucopyranosyl) 6 ,7,3′,4′ tetrahydroxy aurone (8) ,(Z ) 6 O (3″,4″,6″ triacetyl β D glucopyranosyl) 6 ,7,3′,4′ tetrahydroxy aurone (9) ,木犀草素 (luteolin ,1 0 ) ,槲皮素 (quercetin,1 1 ) ,异槲皮苷 (iso quercitrin,1 2 ) ,芦丁 (rutin,1 3) ,黄芪苷 (astragalin,1 4 ) ,quercetin 3,4′ dimethylether 7 O rutinoside (1 5) ,反式丁烯二酸 (1 6) ,2 β D glucopyranosyloxy 1 hydroxy trideca 3 ,5,7,9,1 1 pentayne (1 7)和 3 β D glucopyranosyloxy 1 hydroxy 6 (E ) tetradecene 8,1 0 ,1 2 triyne (1 8)。     

白粉藤的木脂素和三萜成分

从白粉藤（Cissus repens Lank）地上部分分离得到5个木脂素和8个三萜,其中一个木脂素是新化合物,它的结构通过波谱分析和碱水解的方法鉴定为：（＋）-异落叶松树脂醇-9′-（2-对-香豆酰）-O-β-D-吡喃木糖苷（1）。其余化合物分别是：（＋）-异落叶松树脂醇-9′-O-β-D-吡喃木糖苷（2）,（＋）-Lyoniside（3）,（—）-开环异落叶松树脂醇-9-O-β-D-吡喃木糖苷（4）,（7′R,8′S）-4′-hydroxy-3′,5-dimethoxy-7,8′-dihydrobenzofuran-1-propanolneolignan-9′-O-β-D-xylopyranoside（5）,木栓酮（6）,表木栓醇（7）,蒲公英赛醇乙酸酯（8）,熊果酸（9）,2α-羟基乌索酸（10）,积雪草酸（11）,Niga-ichigoside F1（12）,羽扇豆醇（13）。这些化合物都是首次从该植物中分离得到。     

珍珠荚蒾的化学成分研究

从珍珠荚蒾(Viburnum foetidum var.ceanothoides)的枝叶中分离得到14个化合物,经鉴定分别为:白桦醇(1),熊果醇(2),β-谷甾醇(3),白桦脂酸(4),熊果酸(5),对羟基苯甲酸(6),4,4′-二羟基-a-古柯间二酸(7),反式对香豆酸(8),顺式对香豆酸(9),红花菜豆酸(10),原儿茶酸(11),胡萝卜苷(12),1-O-(6-O-α-L-rhamnopyranosyl-β-D-glucopyranosyl) -4-allylbenzene(13)和apigenin 7-O-α-L-rhamnopyranosyl(1″ ′→2″)-β-D-glucopyranoside (14).其中,化合物1、7、9、10和13为首次从荚蓬属中分离得到;所有化合物均首次从珍珠荚蓬中分离得到.