展毛野牡丹酚类化学成分的研究

为了明确展毛野牡丹的化学成分,该研究采用Diaion HP20SS、MCI gel、Sephadex LH-20柱层析和反相高效液相色谱等方法,对展毛野牡丹根和茎的醇提物分别进行了分离纯化。结果表明:从展毛野牡丹中分离得到11个化合物,它们的结构经波谱数据分析和鉴定。它们分别是4-羟基-3-甲氧基苯酚1-O-β-D-(6'-O-没食子酰)-吡喃葡萄糖苷(1)、3,4-二羟基苯乙醇4-O-β-D-(6'-O-没食子酰基)-吡喃葡萄糖苷(2)、龙胆酸5-O-β-D-(6'-O-没食子酰基)-吡喃葡萄糖苷(3)、2,4,6-三甲氧基苯酚1-O-β-D-(6'-O-没食子酰)-吡喃葡萄糖苷(4)、甲基6-O-没食子酰基-β-D-吡喃葡萄糖苷(5)、乙基6-O-没食子酰基-β-D-吡喃葡萄糖苷(6)、6'-O-没食子酰基黑樱苷(7)、没食子酸甲酯(8)、没食子酸乙酯(9)、2,6-二甲氧基对苯二酚4-O-β-D-吡喃葡萄糖苷(10)、2-甲氧基对苯二酚4-O-β-D-吡喃葡萄糖苷(11)。所有化合物均为首次从展毛野牡丹中分离得到,化合物2-7、10和11为首次从该属植物中分离得到。     

珍珠菜的化学成分研究(英文)

从珍珠菜(Lysimachia clethroides)根部分离得到8个化合物,通过波谱数据结合理化性质分别鉴定为山奈素-3-O-β-D-吡喃葡萄糖苷(1),山奈素-3-O-β-D-(2-O-β-D-吡喃葡萄糖)吡喃葡萄糖苷(2),(+)-儿茶素(3),(-)-表儿茶素(4),(+)-没食子儿茶素(5),(-)-表没食子儿茶素(6),(E)-2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucopyranoside(7)和2,3,5,4′-tetrahydroxystilbene-3-O-β-D-glucopyranoside(8)。所有化合物均为首次从该植物中分离得到。     

紫娟茶化学成分及其抗炎抗氧化活性研究

本文对紫娟茶的化学成分进行分离纯化及其抗炎、抗氧化活性进行考察。实验采用现代分离纯化方法,从紫娟茶中共分离得到12个化合物,根据波谱学数据进行结构鉴定为:山柰酚(1)、(+)-表儿茶素(2)、(-)-表儿茶素-3-O-没食子酸酯(3)、杨梅素(4)、表没食子儿茶素-3-O-(3″-O-甲基)没食子酸酯(5)、槲皮素(6)、没食子酸(7)、表没食子儿茶素没食子酸酯(8)、3-O-没食子酰基奎宁(9)、小木麻黄素(10)、1,4,6-三-O-没食子酰基-β-D-葡萄糖(11)、咖啡因(12),其中化合物9为首次从紫娟茶中分离得到。化合物11表现出良好的抗氧化活性,化合物1、3、4、6、8～11均具有显著的抗炎活性。     

鹿蹄草化学成分研究

从鹿蹄草(Pyrola callianthaH.Andres)中分离得到11个化合物,经光谱分析确定其结构为(4R)-1-四氢萘酮(1),(4S)-1-四氢萘酮(2),夹竹桃麻素(3),没食子酸(4),3,4-二羟基苯甲酸(5),鹿蹄草素(6),5-羟甲基糠醛(7),金丝桃苷(8),2″-O-没食子酰基金丝桃苷(9),鹿蹄草苷B(10),4-羟基-2,7-二甲基萘基-1-O-β-D-吡喃葡萄糖苷(11)。其中化合物1,2,3,5,6,11为首次从该植物中分离得到,化合物7为首次从该属中分离得到。     

中华山蓼化学成分研究

bk中华山蓼(Dxyria sinensis Hemsl.)全草中首次分离得到十四个化合物,通过波谱学方法分别鉴定为(-)-表没食子儿茶素(1)、(-)-儿茶素没食子酸酯(2)、(-)-表儿茶素(3)、(-)-表儿茶素-3-没食子酸酯(4)、槲皮素-3-O-α-L-鼠李糖苷(5)、tiliroside(6)、山萘酚-3-O-β-D-吡喃葡萄糖苷(7)、桷皮素(8)、2,4-二羟基-6-甲氧基-乙酰基苯-4-O-β-D-吡喃葡萄糖苷(9)、反-N-(4-羟基苯乙基)阿魏酸酰胺(10)、顺-N-(4-羟基苯乙基)阿魏酸酰胺(11)、icariol A2(12)、(+)-松脂素(13)、Helonioside B(14).     

米碎花的酚苷类化合物及其鼻咽癌细胞毒活性

为了解米碎花(Eurya chinensis)的化学成分及其生物活性,运用多种色谱技术从其乙醇提取物分离得到11个化合物,并对化合物进行体外抗鼻咽癌细胞增殖活性评价。经波谱数据分析,分别为异落新妇苷(1)、3,5,7-三羟基色原酮-3-O-α-L-鼠李糖苷(2)、1-O-反式-桂皮酰基-β-D-葡萄糖(3)、1-O-(4-羟基苯乙基)-6-O-反式-桂皮酰基-β-D-葡萄糖(4)、eutigoside D (5)、1-O-(3,4-二羟基苯乙基)-6-O-反式-香豆酰基-β-D-葡萄糖(6)、eutigoside A (7)、1-O-(4-羟基苯乙基)-6-O-反式-咖啡酰基-β-D-葡萄糖(8)、grayanoside A (9)、1-O-(4-羟基苯乙基)-6-O-(4-羟基苯甲酰基)-β-D-葡萄糖(10)、3-O-β-D-葡萄糖基-4-羟基-苄基苯甲酸酯(11)。其中,化合物4为首次从天然来源获得,化合物2～4和8～11均为首次从该属植物中分离得到。MTT法表明,化合物10具有中等抑制5-8F细胞增殖活性。     

南岭栲叶多酚类化学成分的分离与鉴定

采用Sephadex LH-20、MCI gel CHP 20P和Toyopearl Butyl-650C等柱色谱及半制备液相色谱技术,从壳斗科(Fagaceae)锥属(Castanopsis)植物南岭栲(Castanopsis fordii Hance)叶子乙醇提取物中分离得到18个多酚类化合物,运用波谱学方法解析鉴定为:没食子酸(1)、没食子酸甲酯(2)、没食子酸乙酯(3)、莽草酸-3-O-没食子酸酯(4)、3,3'-二甲基鞣花酸(5)、3,3'-二甲氧基鞣花酸-4'-O-α-D-吡喃木糖苷(6)、3-甲氧基-4'-鼠李糖鞣花酸(7)、6-O-没食子酰基熊果苷(8)、龙胆酸5-O-β-D-(6'-O-没食子酰基)-吡喃葡萄糖(9)、4-hydroxy-3-methoxyphenol 1-O-β-D-(6'-O-galloyl)glucoside(10)、1,6-二-O-没食子酰基-β-D-葡萄糖苷(11)、gallic acid 3-O-β-D-(6'-Ogalloyl)-glucopyranoside(12)、benzyl 6-O-galloyl-β-D-glucopyranoside(13)、2,3-di-O-galloyl-D-glucose(14)、gemin D(15)、特里马素(16)、丁香素(17)、viburnolide A(18)。所有化合物均为首次从该植物中分离得到。     

抱茎苦荬菜水溶性成分的分离与结构鉴定

从抱茎苦荬菜的水溶性成分分离得到5个化合物,根据理化性质和光谱数据分别鉴定为木犀草素-7-O-β-D-葡萄糖醛酸苷(1),木犀草素-7-O-β-D-葡萄糖1→2葡萄糖苷(2),芹菜素-7-O-β-D-葡萄糖醛酸苷(3),菊苣酸(4),(-)3,4-二羟基咖啡酰基酒石酸(5)。化合物3～5为首次从该植物中分离得到。     

来江藤的苯丙素类配糖体成分

从云南民间药用植物来江藤(Brandisia hancei Hook．f．)的全草中分离到10个化合物,分别鉴定为3,4-二羟基苯乙醇基-O-α-L-鼠李吡喃糖基(1→3)-β-D-(4-O-咖啡酰基)-半乳吡喃糖苷(1),3,4-二羟基苯乙醇基-O-α-L-鼠李吡喃糖基(1→3)-β-D-(2-O-乙酰基-4-O-咖啡酰基)-半乳吡喃糖苷(2),acteoside(3),2’-acetylacteoside(4),poliumoside(5),2’-acetylpohumoside(6),mussaenoside(7),luteolin-7-O-β-D-glucoside(8),luteolin(9)和mannitol(10)。化合物1-6为苯丙素配糖体成分,其中化合物2为新化合物,化合物1为首次从该属植物中获得。关键词：来江藤;苯丙素类配糖体;3,4-二羟基苯乙醇基-O-α-L-鼠李吡喃糖基(1→3)-β-D-(2-O-乙酰基-4-O-咖啡酰基)-半乳吡喃糖苷。     

地胆草的化学成分研究

对地胆草全草的化学成分进行研究。采用硅胶柱色谱、Sephadex LH-20以及半制备型高效液相色谱技术进行分离纯化,通过1D、2D NMR,MS等方法鉴定化合物的结构。结果从地胆草95%乙醇提取物中分离并鉴定了9个化合物,分别为1-[(2R*,3S*)-3-ethoxy-2,3-dihydro-6-hydroxy-2-(1-methylethenyl)-1-benzofuran-5-yl]ethanone(1)、7-hydroxy-6-acetyl-2-methylchromone(2)、matriisobenzofuran(3)、桦木酸(betulinic acid,4)、木犀草素(luteolin,5)、木犀草素-7-O-β-D-葡萄糖苷(luteolin-7-O-β-D-glucoside,6)、对香豆酸(p-coumaric acid,7)、3,4-二羟基苯甲醛(3,4-dihydroxy benzaldehyde,8)、豆甾醇-3-O-β-D-葡萄糖苷(stigmasterol-3-O-β-D-glucoside,9)。其中化合物1~3均为首次从该属植物中分离得到,化合物3在国内未见从其它植物中分离报道。     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection

Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions.     

Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen’s Encephalitis

Rasmussen’s encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and 16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis, intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8⁺ T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8⁺ T cells are involved in the pathogenesis of RE.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).