四川地区宫颈癌HPV59型感染及其E6基因突变

宫颈癌是妇科常见的恶性肿瘤,特定型别的人 乳头瘤病毒(HPV)感染是引发宫颈癌的主要因 素。根据HPV致癌性的不同可分为高危型,低 危型。Munoz N[2]等对来自9个国家的1918例宫 颈癌组织进行各型HPV筛查,最终确认HPV-16、 18、26、31、33、35、39、45、51、52、53、56、58、59、66、 68、73及82型为高危型。李洁等[3]用核酸印迹技 术对1986至1994年间来自我国14个省市自治区 的1008宫颈癌组织进行HPV型别检测,发现 HPV-16、18、31、35、51、58等型的存在,并发现各 地区主要流行型别随地理位置有所差异。     

新疆维族妇女感染的HPV型别分布的研究

目的:探讨人乳头瘤状病毒(Human papillomavirus,HPV)在新疆南部维族妇女人群中的型别分布情况.方法:以年龄在30-59岁的新疆伽师县夏普吐勒乡维吾尔族妇女人群为基础进行筛查,签署知情同意书后,采集受试者宫颈脱落细胞,利用PCR和基因芯片技术检测HPVDNA并分型.结果:共2473名妇女入选.HPV总的感染率为9.1%,高危型中HPV-16的感染率最高为6.9%,其他高危型的感染率从高到低依次为HPV-59、HPV-56、HPV-18、HPV-33、HPV-58、HPV-51、HPV-31、HPV-45、HPV-52、HPV-68、HPV-35和HPV-39.低危型中HPV11感染率最高,其他低危型的感染率从高到低依次为HPV-42、HPV-43、HPV-6、HPV-53、HPV-66和HPV-73.HPV-44、HPV-83、HPV-MM4没检测到.多重感染率为34.2%.结论:新疆维吾尔族妇女人群中以HPV16感染为主,其次为HPV59、56、18、33等.HPV59可能是新疆维吾尔族妇女较易感染的类型.体现了新疆维吾尔族妇女感染HPV的特殊性.     

新疆维族妇女感染的HPV 型别分布的研究

目的:探讨人乳头瘤状病毒(Human papillomavirus,HPV)在新疆南部维族妇女人群中的型别分布情况。方法:以年龄在30-59岁的新疆伽师县夏普吐勒乡维吾尔族妇女人群为基础进行筛查,签署知情同意书后,采集受试者宫颈脱落细胞,利用PCR和基因芯片技术检测HPV DNA并分型。结果:共2473名妇女入选。HPV总的感染率为9.1%,高危型中HPV-16的感染率最高为6.9%,其他高危型的感染率从高到低依次为:HPV-59、HPV-56、HPV-18、HPV-33、HPV-58、HPV-51、HPV-31、HPV-45、HPV-52、HPV-68、HPV-35、HPV-39。低危型中HPV11感染率最高,其他低危型的感染率从高到低依次为HPV-42、HPV-43、HPV-6、HPV-53、HPV-66、HPV-73。HPV-44、-83、-MM4没检测到。多重感染率为34.2%。结论:新疆维吾尔族妇女人群中以HPV16感染为主,其次为HPV59、56、18、33等。HPV59可能是新疆维吾尔族妇女较易感染的类型。体现了新疆维吾尔族妇女感染HPV的特殊性。     

MALDI-TOF-MS技术检测宫颈癌中HPV感染状态

目的:本研究拟评MALDI-TOF-MS技术在HPV分型中的实际应用效果.方法:采用MALDI-TOF-MS技术检测河南安阳地区94例宫颈癌样本中HPV感染状态,并与普通PCR方法得到的结果相比较.结果:94例宫颈癌组织中HPV感染率为92.6%,共检测到10种常见的高危型别,以16为主,其次是58和18型.样本单一HPV型别感染为主,其中16型单一型别感染占6I.7%,见多种型别和组合的多重感染.与普通PCR检测结果比较发现两种方法样本HPV(不分型)检测一致率为95.7,样本HPV16型检测一致率为93.6%.结论:MALDI-TOF-MS技术能够精确、快速、高通量的对宫颈癌中HPV进行检测和分型.     

人乳头状瘤病毒感染与肺癌关系的研究进展

近年越来越多的研究发现肺癌组织中有人乳头状瘤病毒（human papillomavirus,HPV）DNA和癌蛋白的检出,其检出率具有地域差异性,但不同地方HPV检出亚型具有高度的一致性,主要是高危型HPV-16和HPV-18。HPV可能主要通过血液循环从宫颈癌病灶等原发部位转移到肺,从而可能诱发肺癌的发生。HPV诱发肺癌的机制可能与影响p53的失活及端粒酶的激活等有关。     

13933例妇科门诊患者人乳头瘤病毒感染状况分析

目的探讨河北医科大学第四医院妇科门诊患者人乳头瘤病毒(HPV)感染情况及型别分布情况,为预防HPV感染和宫颈癌防治提供依据。方法采用核酸分子快速导流杂交技术对13 933例妇科门诊患者的宫颈细胞标本进行21种HPV亚型的基因检测,不同年龄组HPV感染率比较采用卡方检验。结果 13933例受检者中,HPV感染5 979例,感染率为42.9%。共计检出21种亚型,9 023例次HPV感染,其中HPV高危型7 823例次占86.7%,感染前三位分别为HPV-16、HPV-52、HPV-58;HPV低危型1 200例次占13.3%,感染前三位分别为HPV-CP8304、HPV-11、HPV-6;17~25岁和≥56岁年龄组HPV感染率相对较高,与其他年龄组比较差异有统计学意义。结论妇科门诊患者HPV感染率较高,且以高危型HPV感染为主,其中最常见的感染亚型是HPV-16、HPV-52、HPV-58。不同年龄组HPV感染率差异有统计学意义,17~25岁和≥56岁年龄组HPV感染率相对较高。     

宫颈癌组织人乳头瘤病毒的荧光偏振基因分型

采用荧光偏振人乳头瘤病毒(human papillomavirus,HPV)分型新方法探讨了8种常见型别HPV在陕西宫颈癌患者中的流行情况。首先,用HPV GP5 ／GP6 通用引物PCR扩增65例早期宫颈癌(Ⅱa期内)和72例慢性宫颈炎病变组织DNA粗提物,继之将模板指导的末端延伸反应与荧光偏振检测技术结合(TDI-FP),用GP5 ／GP6 扩增区内的HPV6、11、16、18、31、33、35和58型特异性探针与PCR产物杂交后,荧光素标记的特异碱基(TAMRA-ddTTP或R110-ddGTP)在GP5 ／GP6 产物中相应的模板指导下,掺入延伸至相应探针末端,致使对应的TAMRA或R110 FP值升高,从而对扩增的HPV阳性产物进行HPV分型。65例宫颈癌患者中检出HPV57例,阳性率87．69％,72例慢性宫颈炎患者中检出HPV28例,阳性率38．89％,两组间HPV阳性率有显著性差异。宫颈癌与慢性宫颈炎患者中4种最常见的HPV型别分别是HPV 16(45．6％)、HPV 18(22．8％)、HPV 58(17．5％)、HPV 31(7．02％)和HPV 16(35．7％)、HPV 11(32．1％)、HPV 6(21．4％)、HPV 18(10．7％)。慢性宫颈炎患者中检出的HPV型别57．14％属高危型。HPV 16在两组中均最为多见。中国陕西宫颈疾病患者中HPV感染有其特点,世界范围内少见的HPV 58在陕西宫颈癌与慢性宫颈炎患者中均较为多见,在进行HPV新诊断方法及疫苗研制时应考虑到这种特点。     

宫颈癌患者人乳头瘤病毒(HPV)主要型别及其感染研究

本文探讨了江西省和广东省宫颈癌患者人乳头瘤病毒(Human papillomavirus,HPV)感染及其型别分布,分析了高危型HPV对各种宫颈病变的感染情况,为宫颈癌的早期发现和临床诊治提供科学依据。首先采用细胞学、HPV DNA检测(第二代杂交捕获法,HC2)、电子阴道镜和宫颈化学着色方法筛查宫颈癌患者,经病理镜检确诊,然后用GP PCR-SBT法对宫颈癌患者进行HPV基因分型。江西省溪口镇、古市镇及修水县城宫颈癌癌前病变发生率为5．7‰。HC2方法发现宫颈癌患者13种高危型HPV DNA阳性率为89．9％,宫颈上皮内瘤样病变的为84．8％,对照组为24．5％。采用GP PCR-SBT方法进行基因分型发现,江西省宫颈癌患者存在HPV16、58、31、33、18、66、6、11、56和81十种型别,其中HPV81型在国内外鲜有报道。据此提出生殖道高危型HPV感染是妇女宫颈癌发病的重要因素。并发现江西省宫颈癌高发区妇女高危型HPV感染率为24．5％。建立了HPV基因分型的方法,对HPV致宫颈病变的分子机制进行了分析。     

南京部分地区女性宫颈HPV感染状况与分析

目的:探讨南京部分地区女性人乳头瘤病毒(HPV)感染的型别和年龄特征,为宫颈癌的防治提供参考资料.方法:采用人乳头状瘤病毒的基因扩增技术(HPV PCR)对2010年9月至2011年7月3382例就诊于南京妇幼保健院宫颈疾病科的女性进行HPV基因亚型检测,21种型别包括15种高危型和6种低危型,分析比较上述样品的感染程度、感染型别、感染年龄等.结果:HPV总阳性率为33.47％(1132/3382),21种型别均被检出,该地区女性阳性率最高的型别是HPV16,其他常见为58、52、CP8304、53、18、33等,多重感染率为9.58％.该地区女性HPV阳性率与各年龄组无显著差异(P＞0.1).结论:该地区女性HPV阳性率为33.47％,常见型别为HPV16、58、52、CP8304、53、18、33、68、31、39、11、6和66型;人群感染存在在＜20岁和＞70岁两个高峰.     

高危型人乳头瘤病毒基因多态性研究进展

高危型人乳头瘤病毒已被证实是宫颈癌发生发展的必要因素,但不同型别的流行特征存在广泛的区域差异性,且即使是同一型别,其基因组同样存在基因多态性.这些基因多态性可能导致蛋白质功能的差异,进而造成病毒生存能力,致病能力以及与宿主细胞相互作用的差异.现已有大量研究表明HPV16和HPV18型基因多态性在病毒的生物学功能和导致临床疾病进展具有重要意义,因此更多的相关研究围绕着HPV高危型中不同型别的基因多态性相继开展,本文对高危型人乳头瘤病毒HPV16,HPV18,HPV58,HPV52,HPV31和HPV33型的基因多态性研究现状加以综述.     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Synthesis and antimicrobial studies of hydrazone derivatives of 4-[3-(2,4-difluorophenyl)-4-formyl-1H-pyrazol-1-yl]benzoic acid and 4-[3-(3,4-difluorophenyl)-4-formyl-1H-pyrazol-1-yl]benzoic acid

Microbial resistance to antibiotics is an unresolved global concern, which needs urgent and coordinated action. One of the guidelines of the Centers for Disease Control and Preventions (CDC) to combat antibiotic resistance is the development of new antibiotics to treat drug-resistant bacteria. In our effort to find new antibiotics, we report the synthesis and antimicrobial studies of 30 new pyrazole derivatives. These novel molecules have been synthesized by using readily available starting materials and benign reaction conditions. Some of these molecules have shown activity with MIC values as low as 0.78?µg/mL against four bacterial strains; Staphylococcus aureus, methicillin-resistant S. aureus, Bacillus subtilis, and Acinetobacter baumannii. Furthermore, active molecules are non-toxic to mammalian cell line.

     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Novel compounds with potent CDK9 inhibitory activity for the treatment of myeloma

Cyclin-dependent kinases (CDKs) and Polo-like kinases (PLKs) play key role in the regulation of the cell cycle. The aim of our study was originally the further development of our recently discovered polo-like kinase 1 (PLK1) inhibitors. A series of new 2,4-disubstituted pyrimidine derivatives were synthesized around the original hit, but their PLK1 inhibitory activity was very poor. However the novel compounds showed nanomolar CDK9 inhibitory activity and very good antiproliferative effect on multiple myeloma cell lines (RPMI-8226).