通过“现代微生物学技术”课程教学培养研究生创新能力的实践

创新能力提升是当前研究生培养的核心目标,为了探索提高硕士研究生创新能力的课程教学新模式,笔者以微生物学相关专业学术型硕士研究生的选修课程——“现代微生物学技术”课程为例,对课程的教学内容、方法、考核方式等开展了探索与实践。授课教师通过案例教学、研讨式课堂和评价方式的改革,不仅让研究生掌握了现代微生物学技术的相关专业知识和学科前沿,更加提升了学生发现问题、分析问题和解决问题的能力,进而提高了研究生的创新能力。     

基于创新能力培养的“微生物学”研究型教学模式探索

微生物学是生命科学的重要组成部分。随着科学技术的飞速发展,微生物学教学内容和教学方法都需要不断革新,以满足高素质人才培养的需求。华中农业大学在微生物学教学过程中积极开展基于创新能力培养的教学改革实践,课堂上通过推崇学科名人,弘扬科学精神;关注社会焦点,激发学习兴趣;引入学科前沿,培养创新意识等积极引导学生学习微生物学相关知识。通过翻转课堂、对分课堂,借助"微助教"微信公众平台等改变传统的教学模式与师生互动的方式,突破学生学习的时空局限,加强师生交流。依托农业微生物学国家重点实验室等高水平的科研平台,鼓励学生进入实验室运用微生物学知识开展科研实践,引导学生参加各类竞赛,实现理论知识学习向实践创新能力的转化。总之,学生不仅能够在"微生物学"课堂上牢固地掌握微生物学知识,创新思维也得到锻炼,创新能力显著增强。     

现代微生物学研究生课程教学改革与实践——专题教学与seminar教学模式相结合

现代微生物学是面向基础医学专业硕士研究生开设的一门专业基础课程,旨在培养学生的基本科研素养和科研创造力,而传统的讲授型授课方式已无法满足新时代医学研究生教育要求。因此,本文通过专题教学与seminar教学模式(“讨论课”教学模式)相结合,设置六大专题板块辅以学生seminar汇报讨论,应用于现代微生物学研究生课程教学,探讨该教学实践的应用效果,全面提升医学研究生的知识理论水平,培养具有创新思维和具备基本科研素质的高层次医学人才,也为医学类专业其他研究生课程的教学改革提供有益参考。     

研究生高级微生物学课程教学改革与实践初探?

从研究生"高级微生物学"课程的定位、研究生学习该课程的目的、知识背景差异及教学过程中的问题等方面分析了目前研究生"高级微生物学"教学中存在的问题。通过教学内容的优化、教材建设、教学方法改革、师资队伍建设以及建立师生交流平台等方面进行教学改革实践,取得了较好的教学效果。     

非专业研究生生物信息学课程教学中存在的问题及对策

生物信息学是一门多学科交叉的核心学科,对生物医学研究和发展有着巨大的推动作用。对非专业研究生生物信息学课程教学进行剖析,阐述了生物信息学课程的适用对象,总结了教学中存在的供需矛盾及课程教学中存在的问题,并提出了相应对策,为进一步提高非专业研究生生物信息学课程教学质量提供参考。     

基于“互联网+”探究型实验教学模式在“微生物学实验”课程中的探索与应用

从"微生物学实验"课程教学内容和方法改革入手,针对中医药高等院校"微生物学实验"教学的新挑战和新要求,以互联网为基础,构建三大网络教学平台,运用多种教学资源和方法,整合实验教学内容,采用模块化实验教学模式,开展以研究型实验为主的实验项目进行教学改革的探索和应用,旨在不断提高"微生物学实验"课程的教学质量,培养医学生的科研思维能力、创新能力和团队协作精神,并提高学生的综合素质。     

中药学专业微生物学教学课程设计探索

对中药学专业微生物学课程教学内容及模式进行优化改革，以期提升课程的教学质量，进一步激发学生的学习兴趣，夯实基础知识和专业技能。首先分析了目前中药学专业开设的微生物学课程中出现的实际问题，其次在总课时不变的前提下，基于中药材及其相关微生物，并结合地方及中医药特色，进行教学内容的调整、教学手段的改革和考核方式的完善等，使该门课程更符合专业发展需求。在理论教学中调整课时比例，突出微生物学基础知识及微生物在药学中的应用两部分内容。在实践教学中进行分层教学，加深学生对课程知识的理解，同时突出目标导向，培养学生综合实践能力。在教学手段方面，采用模块化与其他教学模式相结合的方式，帮助学生构建微生物学相关知识体系，理解并掌握所学知识。此外，增加平时学习情况及实践方面的考核比例，促使学生重视课程学习及实践能力培养。课程优化后，学生的学习难度有所下降，学习目标更加明确，所学的微生物学知识与中药学专业联系更为密切，更加符合人才培养目标。     

基于SPOC+Problem orientation的环境微生物学混合式教学设计与初探

环境微生物学是高校本科环境类专业的重要专业课程,针对该课程在传统教学中存在的问题,以智慧教学工具雨课堂为平台,将小规模限制性在线课程(Small private online course,SPOC)和问题导向(Problemorientation)引入到环境微生物学课程教学,构建线上和线下相结合的混合式教学模式。该教学模式主要由线上和线下教学内容制订、线上和线下教学实施和教学效果考核、评价及反馈3部分组成。该教学模式以学生为主体,以问题为导向,充分激发学生的学习兴趣,赋予学生更多的个性化体验,增强理论课程教学的互动性。初步教学实践表明,SPOC和Problem orientation的有机结合行之有效,能有效提高教学质量和教学效果。该混合式教学模式有助于培养具备环境微生物专业知识和技能的环保领域创新型人才,也可为其它课程教学质量的提高提供参考。     

医学院校研究生分子生物学课程的改革初探*

分子生物学是当前微生物学领域发展最快的学科,其理论与技术已广泛应用于医学等各个领域。为了适应医学院校研究生的培养,我们在紧密联系微生物等相关课程的基础上,不断改进分子生物学理论和实验教学。组织编写了配套教材,并不断地优化教学内容、规范课程管理、更新教学手段等,不仅系统地培训研究生的各项分子生物学技术,更注重培养其独立思考和科研创新能力,提高研究生的分析综合能力。     

应用型大学微生物学课程教学改革与实践

本文探讨在应用型大学背景下对微生物学课程进行改革,突显应用型大学应有特色,培养高等应用型人才。以荆楚理工学院生物工程专业本科学生为研究对象,通过对教学内容的重新编排、微课制作、案例教学、翻转课堂等多种教学手段的综合应用,对微生物学教学模式进行创新与改革。通过实验组与对照组教学成果和问卷调查的结果显示,新的教学模式较传统方法更利于学生对知识的掌握与应用。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Synthesis and antimicrobial studies of hydrazone derivatives of 4-[3-(2,4-difluorophenyl)-4-formyl-1H-pyrazol-1-yl]benzoic acid and 4-[3-(3,4-difluorophenyl)-4-formyl-1H-pyrazol-1-yl]benzoic acid

Microbial resistance to antibiotics is an unresolved global concern, which needs urgent and coordinated action. One of the guidelines of the Centers for Disease Control and Preventions (CDC) to combat antibiotic resistance is the development of new antibiotics to treat drug-resistant bacteria. In our effort to find new antibiotics, we report the synthesis and antimicrobial studies of 30 new pyrazole derivatives. These novel molecules have been synthesized by using readily available starting materials and benign reaction conditions. Some of these molecules have shown activity with MIC values as low as 0.78?µg/mL against four bacterial strains; Staphylococcus aureus, methicillin-resistant S. aureus, Bacillus subtilis, and Acinetobacter baumannii. Furthermore, active molecules are non-toxic to mammalian cell line.

     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Novel compounds with potent CDK9 inhibitory activity for the treatment of myeloma

Cyclin-dependent kinases (CDKs) and Polo-like kinases (PLKs) play key role in the regulation of the cell cycle. The aim of our study was originally the further development of our recently discovered polo-like kinase 1 (PLK1) inhibitors. A series of new 2,4-disubstituted pyrimidine derivatives were synthesized around the original hit, but their PLK1 inhibitory activity was very poor. However the novel compounds showed nanomolar CDK9 inhibitory activity and very good antiproliferative effect on multiple myeloma cell lines (RPMI-8226).