共查询到20条相似文献,搜索用时 343 毫秒
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Deborah L. Petrik Steven D. Karlen Cynthia L. Cass Dharshana Padmakshan Fachuang Lu Sarah Liu Philippe Le Bris Sébastien Antelme Nicholas Santoro Curtis G. Wilkerson Richard Sibout Catherine Lapierre John Ralph John C. Sedbrook 《The Plant journal : for cell and molecular biology》2014,77(5):713-726
Grass lignins contain substantial amounts of p‐coumarate (pCA) that acylate the side‐chains of the phenylpropanoid polymer backbone. An acyltransferase, named p‐coumaroyl‐CoA:monolignol transferase (OsPMT), that could acylate monolignols with pCA in vitro was recently identified from rice. In planta, such monolignol‐pCA conjugates become incorporated into lignin via oxidative radical coupling, thereby generating the observed pCA appendages; however p‐coumarates also acylate arabinoxylans in grasses. To test the authenticity of PMT as a lignin biosynthetic pathway enzyme, we examined Brachypodium distachyon plants with altered BdPMT gene function. Using newly developed cell wall analytical methods, we determined that the transferase was involved specifically in monolignol acylation. A sodium azide‐generated Bdpmt‐1 missense mutant had no (<0.5%) residual pCA on lignin, and BdPMT RNAi plants had levels as low as 10% of wild‐type, whereas the amounts of pCA acylating arabinosyl units on arabinoxylans in these PMT mutant plants remained unchanged. pCA acylation of lignin from BdPMT‐overexpressing plants was found to be more than three‐fold higher than that of wild‐type, but again the level on arabinosyl units remained unchanged. Taken together, these data are consistent with a defined role for grass PMT genes in encoding BAHD (BEAT, AHCT, HCBT, and DAT) acyltransferases that specifically acylate monolignols with pCA and produce monolignol p‐coumarate conjugates that are used for lignification in planta. 相似文献
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Changing a conserved amino acid in R2R3‐MYB transcription repressors results in cytoplasmic accumulation and abolishes their repressive activity in Arabidopsis 下载免费PDF全文
Meiliang Zhou Zhanmin Sun Chenglong Wang Xinquan Zhang Yixiong Tang Xuemei Zhu Jirong Shao Yanmin Wu 《The Plant journal : for cell and molecular biology》2015,84(2):395-403
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Yuri Takeda Shiro Suzuki Yuki Tobimatsu Keishi Osakabe Yuriko Osakabe Safendrri K. Ragamustari Masahiro Sakamoto Toshiaki Umezawa 《The Plant journal : for cell and molecular biology》2019,97(3):543-554
The aromatic composition of lignin is an important trait that greatly affects the usability of lignocellulosic biomass. We previously identified a rice (Oryza sativa) gene encoding coniferaldehyde 5‐hydroxylase (OsCAld5H1), which was effective in modulating syringyl (S)/guaiacyl (G) lignin composition ratio in rice, a model grass species. Previously characterized OsCAld5H1‐knockdown rice lines, which were produced via an RNA‐interference approach, showed augmented G lignin units yet contained considerable amounts of residual S lignin units. In this study, to further investigate the effect of suppression of OsCAld5H1 on rice lignin structure, we generated loss‐of‐function mutants of OsCAld5H1 using the CRISPR/Cas9‐mediated genome editing system. Homozygous OsCAld5H1‐knockout lines harboring anticipated frame‐shift mutations in OsCAld5H1 were successfully obtained. A series of wet‐chemical and two‐dimensional NMR analyses on cell walls demonstrated that although lignins in the mutant were predictably enriched in G units all the tested mutant lines produced considerable numbers of S units. Intriguingly, lignin γ‐p‐coumaroylation analysis by the derivatization followed by reductive cleavage method revealed that enrichment of G units in lignins of the mutants was limited to the non‐γ‐p‐coumaroylated units, whereas grass‐specific γ‐p‐coumaroylated lignin units were almost unaffected. Gene expression analysis indicated that no homologous genes of OsCAld5H1 were overexpressed in the mutants. These data suggested that CAld5H is mainly involved in the production of non‐γ‐p‐coumaroylated S lignin units, common in both eudicots and grasses, but not in the production of grass‐specific γ‐p‐coumaroylated S units in rice. 相似文献
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Jin Zhang Meng Xie Mi Li Jinhua Ding Yunqiao Pu Anthony C. Bryan William Rottmann Kimberly A. Winkeler Cassandra M. Collins Vasanth Singan Erika A. Lindquist Sara S. Jawdy Lee E. Gunter Nancy L. Engle Xiaohan Yang Kerrie Barry Timothy J. Tschaplinski Jeremy Schmutz Gerald A. Tuskan Wellington Muchero Jin‐Gui Chen 《Plant biotechnology journal》2020,18(3):859-871
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Expression of the MYB transcription factor gene BplMYB46 affects abiotic stress tolerance and secondary cell wall deposition in Betula platyphylla 总被引:1,自引:0,他引:1 下载免费PDF全文
Huiyan Guo Yucheng Wang Liuqiang Wang Ping Hu Yanmin Wang Yuanyuan Jia Chunrui Zhang Yu Zhang Yiming Zhang Chao Wang Chuanping Yang 《Plant biotechnology journal》2017,15(1):107-121